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The map label for this gene is rluB [H]

Identifier: 71910124

GI number: 71910124

Start: 317939

End: 318685

Strand: Direct

Name: rluB [H]

Synonym: M5005_Spy_0310

Alternate gene names: 71910124

Gene position: 317939-318685 (Clockwise)

Preceding gene: 71910123

Following gene: 71910125

Centisome position: 17.29

GC content: 39.22

Gene sequence:

>747_bases
TTGCTCAAAAGAAAGAAGAATATAATGCGAATTAACAAATACATTGCCCATGCGGGTATTGCCAGTCGTCGTAAGGCTGA
AGAATTGATTAAGCAAGGCTTAGTAACCTTAAATGGTCAGGTTATTACGGATTTAGCAACCACTGTAAAATCGGGAGACG
TGGTTGAAATAGAAGGAAGTCCTATTTACAACGAGGAAAAGGTTTACTATTTGCTTAATAAACCTCGTGGCGCTATCTCA
AGTGTGTCAGATGACAAAGGCCGTAAAACGGTACTGGATTTACTACCTCAGGTTAAAGAACGCATATACCCTGTTGGACG
ATTGGATTGGGACACATCAGGGGTCCTTATTTTAACGAATGATGGTGATTTTACAGATACTATGATTCATCCTAGAAATG
AAATAGACAAGGTTTATCTGGCGCGTGTCAAAGGTATTGCAACGAAAGAAAATCTTCGTCCCTTAACTCGAGGAATTGTT
ATTGATGGTAAAAAGACCAAACCAGCTCGCTACAATATTGTTCGTGTAGAAGCTGATAAGAGTCGATCGATCGTAGAATT
GACTATCCATGAAGGGCGAAACCATCAAGTGAAAAAAATGTTTGAGTCTGTGGGGCTTCTGGTTGATAAATTATCTCGTA
CACGTTTTGGGACCGTCGACTTAAAGGGACTAAGACCAGGAGAAGCAAGACGCTTAAATAAAAAAGAAATAAGTCAACTG
CATAACTTAGCTAATACGAAAAAATGA

Upstream 100 bases:

>100_bases
GAGTTTTGCTTGTCATCAAATAGCAAATGGCGTTTACAAAAGGATTTGTTTTAAGGCGTTTTAGTTGAAAAAAAAGCCTT
AAACTAGGGACAACAAAAAG

Downstream 100 bases:

>100_bases
TGAAAAAACTATTAATAGTATCTGTTAAAGCTTATCAAAAATACATTTCTCCTTTATCTCCTCCTAGTTGTCGCTACAAG
CCAACCTGTTCAGCTTATAT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLKRKKNIMRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAIS
SVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIV
IDGKKTKPARYNIVRVEADKSRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQL
HNLANTKK

Sequences:

>Translated_248_residues
MLKRKKNIMRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAIS
SVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIV
IDGKKTKPARYNIVRVEADKSRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQL
HNLANTKK
>Mature_248_residues
MLKRKKNIMRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGSPIYNEEKVYYLLNKPRGAIS
SVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIV
IDGKKTKPARYNIVRVEADKSRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQL
HNLANTKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=36.9747899159664, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=33.7552742616034, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=32.3770491803279, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI87081838, Length=173, Percent_Identity=32.3699421965318, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28020; Mature: 28020

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRKKNIMRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEECCC
PIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTN
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
DGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKPARYNIVRVEADK
CCCCCCCEECCCHHHHHHHHHHHHCCCCHHHCCHHHCCEEEECCCCCCCEEEEEEEECCC
SRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHH
HNLANTKK
HHHHCCCC
>Mature Secondary Structure
MLKRKKNIMRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSGDVVEIEGS
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEECCC
PIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDWDTSGVLILTN
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
DGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKPARYNIVRVEADK
CCCCCCCEECCCHHHHHHHHHHHHCCCCHHHCCHHHCCEEEECCCCCCCEEEEEEEECCC
SRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRPGEARRLNKKEISQL
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHCCCHHHHHHH
HNLANTKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]