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The map label for this gene is dacA [H]

Identifier: 71910061

GI number: 71910061

Start: 254605

End: 255786

Strand: Reverse

Name: dacA [H]

Synonym: M5005_Spy_0247

Alternate gene names: 71910061

Gene position: 255786-254605 (Counterclockwise)

Preceding gene: 71910062

Following gene: 71910050

Centisome position: 13.91

GC content: 37.31

Gene sequence:

>1182_bases
TTGACCAAAAGAATTATACTAACTATTTTTACCTTTATTTGCTTTTCGGTGATGCCTCTTGTCCACGCCGAAGATGTCAT
GGATATTACAAGGCAAGCAGGCTACACAGTTAGTGAGGTTAATCGACCAAAATCTTCTATTGTCGTTGATGCAAATAGTT
CAGATATTCTTTGGCAAGATAATATTGATATTCCTAGGGATCCTGCTAGTATGTCTAAGATGTTTACGCTTTACATTTTA
TTTGAAGAGCTCGCTAAGGGAAAAATTACGATGGACACTACTATTACGGCAACTCCAACCGATCAAGCTATCGCTAACAT
TTATGAGATTAGCAATAATAATATCGTCGCTGGAGTAGCTTATCCTATTCGTGATCTTATTACTATGACAGCTGTGCCTT
CATCAAATGCAGCAACTGTTATGATTGCCAATTATTTATCAAACAATGATGCTTCTGCGTTTATCGATCGTGTTAATGCC
ACAGCCAAACAATTAGGCATGACTAACACTCATTTTTCAAACGCTAGTGGTGCAGCAGCACAAGCTTTTCAAGGTTATTA
TAATCCTACTAAATATGATTTATCTGCTTCAAATATTACGACCGCTCGAGATTTGTCCAAGTTACTCTATGCCTTCTTAA
AAAAATACCCTGAAATTATCTCCTTTACAAATAAATCTGTTGTGCACACTATGGTAGGAACGCCATATGAAGAAGAATTT
CACACCTATAACCACTCTCTCCCAGATAATCAATTTGGTATGAAAGGAGTTGATGGATTAAAAACAGGTTCTAGTCCCAG
TGCTGCTTTTAACGCCATGATAACTGCCAAAAGAGGCAAAACTCGACTCATTACTATTGTCATGGGGGTCGGAGATTGGT
CAGATCAAAACGGGGAGTTTTATATACATCCTTTCGTCAATGCCTTGACTGAGAAAGGGTTTAAAGATAGTAAGACCTTA
TCCAAAAAAGCACGCCAAAAACTCGAAAAATTAGTGCCACAAACCAAAAAAGAAACATCATCTAAACAGCAGCACTTCAA
AGCCACTAAAAAGCAATCGTACTTGGAAAGAGTCGAAGATTTTATGAATCACAACCACACTTTCTTGCTTATTTGCTTAG
CTATCTTTATAATTACTATCCTTTTACTAAGCCTCGTTGTCTTTGCAATGGGACGCCAATAA

Upstream 100 bases:

>100_bases
TAAGCAAACTAGGCCAATCCTAACAATTTTTCAAAAAAACTTCACATTGTATTAGCAAAGTGATATACTGAAAATGCATT
ACATTTAAAGGAGTCGGAAA

Downstream 100 bases:

>100_bases
TGAATTATCAAAAACCTCTGTCAAAGCACAGAGGTTTTTGATATGTTATCTTACGTGTTCAAGGCAACTAAAAACTAACC
ACTTGGGGTTAGTACTACAA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPKSSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYIL
FEELAKGKITMDTTITATPTDQAIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNA
TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPEIISFTNKSVVHTMVGTPYEEEF
HTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITAKRGKTRLITIVMGVGDWSDQNGEFYIHPFVNALTEKGFKDSKTL
SKKARQKLEKLVPQTKKETSSKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGRQ

Sequences:

>Translated_393_residues
MTKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPKSSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYIL
FEELAKGKITMDTTITATPTDQAIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNA
TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPEIISFTNKSVVHTMVGTPYEEEF
HTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITAKRGKTRLITIVMGVGDWSDQNGEFYIHPFVNALTEKGFKDSKTL
SKKARQKLEKLVPQTKKETSSKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGRQ
>Mature_392_residues
TKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPKSSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILF
EELAKGKITMDTTITATPTDQAIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNAT
AKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPEIISFTNKSVVHTMVGTPYEEEFH
TYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITAKRGKTRLITIVMGVGDWSDQNGEFYIHPFVNALTEKGFKDSKTLS
KKARQKLEKLVPQTKKETSSKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGRQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=296, Percent_Identity=28.7162162162162, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787062, Length=253, Percent_Identity=28.8537549407115, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082022, Length=252, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082054, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 43748; Mature: 43617

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPKSSIVVDANSSDILWQD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCEEEEC
NIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQAIANIYEISNNNIVAGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCHHHHHHHHEECCCCEEEEEH
YPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNATAKQLGMTNTHFSNASGAAA
HHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPEIISFTNKSVVHTMVGTPYEEEF
HHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHH
HTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITAKRGKTRLITIVMGVGDWSDQNGEF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCEEEEEEEEECCCCCCCCCCE
YIHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQTKKETSSKQQHFKATKKQSYLERVED
EEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMNHNHTFLLICLAIFIITILLLSLVVFAMGRQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRIILTIFTFICFSVMPLVHAEDVMDITRQAGYTVSEVNRPKSSIVVDANSSDILWQD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCEEEEC
NIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQAIANIYEISNNNIVAGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECEEEEECCCHHHHHHHHEECCCCEEEEEH
YPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRVNATAKQLGMTNTHFSNASGAAA
HHHHHHHHHHCCCCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
QAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPEIISFTNKSVVHTMVGTPYEEEF
HHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHH
HTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITAKRGKTRLITIVMGVGDWSDQNGEF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCCCEEEEEEEEECCCCCCCCCCE
YIHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQTKKETSSKQQHFKATKKQSYLERVED
EEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMNHNHTFLLICLAIFIITILLLSLVVFAMGRQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]