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The map label for this gene is nisX1 [H]

Identifier: 71909980

GI number: 71909980

Start: 178801

End: 179016

Strand: Reverse

Name: nisX1 [H]

Synonym: M5005_Spy_0166

Alternate gene names: 71909980

Gene position: 179016-178801 (Counterclockwise)

Preceding gene: 71909981

Following gene: 71909979

Centisome position: 9.74

GC content: 34.26

Gene sequence:

>216_bases
ATGAGTCGAAAAGGTAATCCATACGATAATGCACTCATGGAATCCTTTTATAAAACATTAAAAAGAGAGCTTGTCAATGA
TGCTCATTTCGCAACTATTGAGCAAGCTCAGCTTGAGATTTTTAAATATAGTGAGACTTACTATAACCCTAAACGTCTGC
ATTCAGCTTTAGGGTATCTCTCACCTGTTGAATTTGAAAAAATAGTTACTCATTAG

Upstream 100 bases:

>100_bases
AAAGCCTAAAGAAGGGGTAATCGTCCATACAGATCAGGGTTCTCAATATACCGGAGCATGATTTCAGGACCTACTTAGAC
AGAAAAAATGTAAGTCCAGG

Downstream 100 bases:

>100_bases
CTTAACTTTATGTCTATTTTTTCTTGACAAGTCCAGGGTATGAAGTCCCTTTATACACATACTTTCAAAACAGCTCTCAA
TCCTCTGTCCGCTACAAAAA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 71; Mature: 70

Protein sequence:

>71_residues
MSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSPVEFEKIVTH

Sequences:

>Translated_71_residues
MSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSPVEFEKIVTH
>Mature_70_residues
SRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYLSPVEFEKIVTH

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=66, Percent_Identity=46.969696969697, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1787263, Length=66, Percent_Identity=46.969696969697, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1786753, Length=66, Percent_Identity=46.969696969697, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1786570, Length=66, Percent_Identity=46.969696969697, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1786490, Length=66, Percent_Identity=46.969696969697, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 8374; Mature: 8242

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYL
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
SPVEFEKIVTH
CCHHHHHHHCC
>Mature Secondary Structure 
SRKGNPYDNALMESFYKTLKRELVNDAHFATIEQAQLEIFKYSETYYNPKRLHSALGYL
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
SPVEFEKIVTH
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]