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The map label for this gene is purH [H]

Identifier: 71909841

GI number: 71909841

Start: 44106

End: 45653

Strand: Direct

Name: purH [H]

Synonym: M5005_Spy_0027

Alternate gene names: 71909841

Gene position: 44106-45653 (Clockwise)

Preceding gene: 71909840

Following gene: 71909843

Centisome position: 2.4

GC content: 47.16

Gene sequence:

>1548_bases
ATGACTAAACGTGCTTTAATCTCAGTGTCTGACAAGTCAGGAATTGTTGACTTTGCAAAAGAATTGAAAAACTTGGGTTG
GGATATTATCTCAACTGGTGGGACTAAGGTTACCCTTGACGATGCTGGTGTTGAGACCATTGCCATCGACGATGTGACTA
GATTCCCAGAGATGATGGACGGTCGTGTTAAGACCCTCCACCCAAACATTCACGGTGGGCTTTTGGCTCGTCGCGACGCT
GACAGCCACCTTCAGGCTGCTAAGGACAACAATATCGAGCTGATTGACCTCGTGGTTGTCAACCTCTATCCCTTCAAGGA
GACTATCCTTCGTCCAGACATAACCTATGATTTGGCGGTGGAAAACATCGACATCGGCGGTCCATCAATGCTTCGCTCAG
CCGCTAAAAATCACGCTAGCGTGACCGTTGTGGTTGACCCAGCGGATTATGCGACTGTTTTGGGAGAATTGGCTGACGCT
GGTCAGACGACATTTGAAACGCGTCAACGCTTGGCAGCTAAGGTCTTTCGTCACACAGCAGCCTACGACGCTTTGATTGC
TGAGTACTTCACAACTCAAGTGGGAGAGGCTAAGCCTGAAAAATTAACCATCACTTACGACCTTAAACAGGCTATGCGTT
ACGGAGAAAATCCACAACAAGACGCTGATTTCTATCAAAAAGCCTTGCCAACAGACTACTCAATTGCTTCTGCTAAACAG
CTCAACGGAAAAGAATTGTCTTTCAATAATATCCGTGATGCCGATGCGGCAATCCGTATTATCCGCGATTTCAAAGACCG
TCCAACTGTTGTTGCTCTCAAACACATGAACCCATGTGGTATCGGACAGGCTGATGACATTGAGACTGCTTGGGATTACA
CTTATAAAGCTGACCCAGTTTCAATCTTTGGCGGAATTATTGTCCTTAACCGTGAAGTTGACGCAGCGACAGCCAAGAAG
ATGCACCCTATCTTCTTGGAAATCATCATCGCACCATCATACTCAGAAGAAGCGCTAGCTATTCTCACAAATAAAAAGAA
AAACTTGCGAATCCTTGAGTTGCCGTTTGATGCCCAAGCTGCCAGCGAAGTGGAAGCTGAGTACACTGGCGTAGTTGGTG
GACTTTTGGTGCAAAACCAAGACGTTGTGGCTGAAAATCCATCTGACTGGCAAGTGGTGACAGACCGCCAACCAACAGAA
CAAGAGGCGACTGCCCTTGAGTTTGCCTGGAAGGCTATCAAGTATGTTAAGTCTAACGGGATTATTATTACTAACGATCA
CATGACGCTTGGACTCGGTGCAGGTCAAACCAACCGTGTCGGCTCAGTCAAGATTGCTATCGAGCAGGCTAAAGACCACC
TTGACGGTGCCGTTCTAGCATCAGATGCCTTCTTCCCATTTGCGGACAACATTGAAGAAATCGCTGCCGCAGGGATCAAA
GCAATCATCCAACCAGGTGGTTCAGTTCGTGACCAAGACTCTATTGACGCCGCAAACAAACATGGCTTGACCATGATCTT
CACAGGTGTGAGACATTTTAGGCATTAA

Upstream 100 bases:

>100_bases
TGGAAGGCACAGAAAAAAGATAAACATCTCCTAGGCGCAAGCGTCTTCGTCGAGTTTCCTATTTTTCCTTGGCCTTCTAG
TCGGACTTGGTATCTTAAAT

Downstream 100 bases:

>100_bases
TAAAAAAATGTGGTCTAATTAAAACCACATTTTTTATTAAAAGAAAATATAAGTTAACTATAATTTGTTAGCTTTATCAG
CATTTACCCATGTTCCACGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGVETIAIDDVTRFPEMMDGRVKTLHPNIHGGLLARRDA
DSHLQAAKDNNIELIDLVVVNLYPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYTYKADPVSIFGGIIVLNREVDAATAKK
MHPIFLEIIIAPSYSEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQVVTDRQPTE
QEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQAKDHLDGAVLASDAFFPFADNIEEIAAAGIK
AIIQPGGSVRDQDSIDAANKHGLTMIFTGVRHFRH

Sequences:

>Translated_515_residues
MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGVETIAIDDVTRFPEMMDGRVKTLHPNIHGGLLARRDA
DSHLQAAKDNNIELIDLVVVNLYPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYTYKADPVSIFGGIIVLNREVDAATAKK
MHPIFLEIIIAPSYSEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQVVTDRQPTE
QEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQAKDHLDGAVLASDAFFPFADNIEEIAAAGIK
AIIQPGGSVRDQDSIDAANKHGLTMIFTGVRHFRH
>Mature_514_residues
TKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGVETIAIDDVTRFPEMMDGRVKTLHPNIHGGLLARRDAD
SHLQAAKDNNIELIDLVVVNLYPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADAG
QTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQL
NGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYTYKADPVSIFGGIIVLNREVDAATAKKM
HPIFLEIIIAPSYSEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQVVTDRQPTEQ
EATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQAKDHLDGAVLASDAFFPFADNIEEIAAAGIKA
IIQPGGSVRDQDSIDAANKHGLTMIFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=476, Percent_Identity=36.7647058823529, Blast_Score=253, Evalue=3e-67,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=48.9603024574669, Blast_Score=480, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71985564, Length=477, Percent_Identity=34.1719077568134, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71985556, Length=318, Percent_Identity=23.8993710691824, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71985574, Length=310, Percent_Identity=25.1612903225806, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323768, Length=477, Percent_Identity=38.3647798742138, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=34.8837209302326, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24649832, Length=476, Percent_Identity=37.1848739495798, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56350; Mature: 56219

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGVETIAIDDVTRFPEMMD
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCHHHHHHHHC
GRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNLYPFKETILRPDITYDLAV
CCEEEECCCCCCCEEEECCCHHHHHHCCCCCCEEEEEEEEEEECCHHHHCCCCCEEEEEE
ENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADAGQTTFETRQRLAAKVFRHTA
EEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYTYKADPV
CCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHCCCEECCCCC
SIFGGIIVLNREVDAATAKKMHPIFLEIIIAPSYSEEALAILTNKKKNLRILELPFDAQA
EEECCEEEEECCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCH
ASEVEAEYTGVVGGLLVQNQDVVAENPSDWQVVTDRQPTEQEATALEFAWKAIKYVKSNG
HHHHHHHHHHHEEHEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
IIITNDHMTLGLGAGQTNRVGSVKIAIEQAKDHLDGAVLASDAFFPFADNIEEIAAAGIK
EEEECCCEEEEECCCCCCCCCEEEEEEEHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
AIIQPGGSVRDQDSIDAANKHGLTMIFTGVRHFRH
HHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
TKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVTLDDAGVETIAIDDVTRFPEMMD
CCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEECCHHHHHHHHC
GRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNLYPFKETILRPDITYDLAV
CCEEEECCCCCCCEEEECCCHHHHHHCCCCCCEEEEEEEEEEECCHHHHCCCCCEEEEEE
ENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADAGQTTFETRQRLAAKVFRHTA
EEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQ
HHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
LNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYTYKADPV
CCCCEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHCCCEECCCCC
SIFGGIIVLNREVDAATAKKMHPIFLEIIIAPSYSEEALAILTNKKKNLRILELPFDAQA
EEECCEEEEECCCCHHHHHCCCCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCH
ASEVEAEYTGVVGGLLVQNQDVVAENPSDWQVVTDRQPTEQEATALEFAWKAIKYVKSNG
HHHHHHHHHHHEEHEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
IIITNDHMTLGLGAGQTNRVGSVKIAIEQAKDHLDGAVLASDAFFPFADNIEEIAAAGIK
EEEECCCEEEEECCCCCCCCCEEEEEEEHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
AIIQPGGSVRDQDSIDAANKHGLTMIFTGVRHFRH
HHCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA