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The map label for this gene is leuS [H]

Identifier: 71893995

GI number: 71893995

Start: 847459

End: 849831

Strand: Reverse

Name: leuS [H]

Synonym: MHJ_0647

Alternate gene names: 71893995

Gene position: 849831-847459 (Counterclockwise)

Preceding gene: 71893998

Following gene: 71893994

Centisome position: 94.7

GC content: 30.85

Gene sequence:

>2373_bases
ATGTTAGATCACCGAGCTATTGAAAAAAAGTGACAAGATTACTGGTATAAAAATAAGGTTTTCAAAACAACCGAGACATC
TAGAAAAAAAGCCTATATTCTCGATATGTTTCCCTATCCTTCGGCTTCTGGTCTGCATCTAGGCCATCCGATTGGCTATA
CGGCTTCTGATATTGTTGCTAGATTTAAACGTTTAAATGGTTTTGACGTGCTTCATCCGATGGGTTGGGATGCTTTTGGT
TTGCCTGCAGAGCAATATGCGATAAAAACAGGTAATCATCCGGGTGAATTTACAAAAAGAAATATTGAAAATTTTAAAAA
ACAAATAATTTCTTTTGGATTTTCTTATGATTGGGATAAGGAAATTAACACCTCTGATCCAGAATTTTATGTCCAAACTC
AGTGGATTTTTAAACTATTATATAAGGCAAATTTAGCTGAGATTCGCGAAGTTGAAGTCAATTGATGCCCGGAATTAGGC
ACTGTTTTAGCAAATGAGGAATTAAAAAGGGAAAAAAATGGTGCGCTTGTCAGTGAAAGAGGAAATTTTCCTGTAATTTT
AAAAAAAATGAAACAGTGAGTTTTAAAAATCACTGAATATGCACAGAAATTACTTGATGGACTTGATCAAGTTGATTTTC
CTGATTCTTTAAAAACTTTACAAAAAAAATGAATCGGTAAATCAAAAGGGTGAAAAATTAAATTTTTAATTGAAAAAACC
GGTGAATTTCTTGAAATTTTTACAACAAAAATCGAAACTTTTTATGGAGTAAGTTTCCTAGCCCTTGCACCTTTACATAA
TTTTGCTCAAAAATTGGCTAAAAATAAGGAAAAAATCAGCCAATTTATTGAGCAAAACAGCTTTTCAAGTCCAAAATTAC
AAAGTAAAATGACAGGAATTTTCACTGGATTATATGCGGTTCATCCTTTAAATTCAAGTAAAATTCCGATTTATATTACA
AATTATGTTCTAAATAACTATGCAACTTCTGCAATTATGGGGGTTCCTGCCCATAATTTAGCTGATTTAGAATTTGCTCA
AATTTTCCAAATTGATTATGTTAAAGTAATAAATCAAGAAAATTTACTTATAAATTCTGCAGAATTTTCTGGTTTGACTA
TTAAAGAGGGAGCAAATTCTATTTTCAGTAAGCTAAATTCAATGAATTTAGTTCAAAAAACTACAATTTTTCGACTAAAA
GATTGAGTTTTTTCCCGTCAGAGATACTGAGGAGAACCTTTTCCGGTTTATTTTGATGAAAAAGGAAAGATTTATCTTGA
AGAGAAAATAGTTGAGCTCCCACATACTGATAAAATTATGCCTTCTGGTGATGGCCAGTCCCCACTTGCATCGATAAAAG
ACTGAGTTTTCTTTGAAAAAAATGGGAAATACTACCGCCGGGAAACAAATACAATGCCACAATGAAGCGGAAGCTCATGG
TATTATCTAGCTTTTATTTTAAAAGAAAAAGATAAATATTTGCCGCTTGATTCAAAAATTGCCTATGATAAATTCAAAAA
ATGGCTGCCAGTTGATCTTTATATAGGGGGTCAGGAGCATGCGGTTGGCCATTTAATTTATTCCCGTTTTTGACATAAGG
TTTTATTTGATGCGGGGATAGTTCCAAATTCTGAACCTTTTCAAAAAATAATTCATCAAGGAATGTTACTAGGTTCTGAT
GGTCAAAAAATGTCGAAATCTAAAGGAAATACGATAAATCCTACGGAAATCATAGACAAATTTGGCGCTGATACAATTAG
AATTTTTTTAATGTTTATGGGTCCGATTGGGGATCTTCGACTTTGAGATGAAAATGGAGTAGTTTCAATTTTTAACTGAA
TTCAACGGGTACAAAGAACAATTTTAGCTGACTATAAAATTGACCCCGAACTTGAAAAAGACTATGAATTTACGTATTTT
TTCAATAATTTTGTTTTCGAAATCACAAATTTAATCGAAACTTTTAAGTTCAATGTGGCAATTTCAAAATTAATGGTTTA
CATTAATTTTCTAAATAAAAAGCAAAAAATTCCTTCAAAAAATTATCTGACAGATTTTTTAATTATTTTCTCAGTTTTTG
CACCGCATACAGCAGAAGAATTACTTGAAAAATTAGGACAAAAACCCTTAAATTTCCAGATTTGACCTCAATTTGATCGG
TCAAAAATTTTTAAAAATACTTTTCATTTACCTGTTTCAATTAATGGGAAAAACCGCGGAATTCTCGAATTAACAGATGA
AAAATCACAAGAAGAAATCATCGAGATTGCCAAAAAAGACTATAAAATACGTAACTATTTGCAAAATAAGCAAATAATAA
AAGTCATTTATATCCCGAGAAAAATTCTAAATTTTATTCTCAAAAATAATTAA

Upstream 100 bases:

>100_bases
GGAATTAGTACTTTTTTAAATATTTCTAATTTTTCCTTTTTAGAAAAGTCACTTTTGGCTTTTAATTTTAAAAATTTATG
CTATAATTTAGGTAACTATC

Downstream 100 bases:

>100_bases
AATATGAAAAAATTTTTATTTCCCTTTTCAGTTTCATTTATAAGCTTTTTAGCAGAAATTTTGACCTTGTTATTAAAGGT
TATTTTTTATGATAGTTTTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 790; Mature: 790

Protein sequence:

>790_residues
MLDHRAIEKKWQDYWYKNKVFKTTETSRKKAYILDMFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFG
LPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFSYDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVNWCPELG
TVLANEELKREKNGALVSERGNFPVILKKMKQWVLKITEYAQKLLDGLDQVDFPDSLKTLQKKWIGKSKGWKIKFLIEKT
GEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKNKEKISQFIEQNSFSSPKLQSKMTGIFTGLYAVHPLNSSKIPIYIT
NYVLNNYATSAIMGVPAHNLADLEFAQIFQIDYVKVINQENLLINSAEFSGLTIKEGANSIFSKLNSMNLVQKTTIFRLK
DWVFSRQRYWGEPFPVYFDEKGKIYLEEKIVELPHTDKIMPSGDGQSPLASIKDWVFFEKNGKYYRRETNTMPQWSGSSW
YYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVGHLIYSRFWHKVLFDAGIVPNSEPFQKIIHQGMLLGSD
GQKMSKSKGNTINPTEIIDKFGADTIRIFLMFMGPIGDLRLWDENGVVSIFNWIQRVQRTILADYKIDPELEKDYEFTYF
FNNFVFEITNLIETFKFNVAISKLMVYINFLNKKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQIWPQFDR
SKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIEIAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN

Sequences:

>Translated_790_residues
MLDHRAIEKK*QDYWYKNKVFKTTETSRKKAYILDMFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFG
LPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFSYDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVN*CPELG
TVLANEELKREKNGALVSERGNFPVILKKMKQ*VLKITEYAQKLLDGLDQVDFPDSLKTLQKK*IGKSKG*KIKFLIEKT
GEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKNKEKISQFIEQNSFSSPKLQSKMTGIFTGLYAVHPLNSSKIPIYIT
NYVLNNYATSAIMGVPAHNLADLEFAQIFQIDYVKVINQENLLINSAEFSGLTIKEGANSIFSKLNSMNLVQKTTIFRLK
D*VFSRQRY*GEPFPVYFDEKGKIYLEEKIVELPHTDKIMPSGDGQSPLASIKD*VFFEKNGKYYRRETNTMPQ*SGSSW
YYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVGHLIYSRF*HKVLFDAGIVPNSEPFQKIIHQGMLLGSD
GQKMSKSKGNTINPTEIIDKFGADTIRIFLMFMGPIGDLRL*DENGVVSIFN*IQRVQRTILADYKIDPELEKDYEFTYF
FNNFVFEITNLIETFKFNVAISKLMVYINFLNKKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQI*PQFDR
SKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIEIAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN
>Mature_790_residues
MLDHRAIEKK*QDYWYKNKVFKTTETSRKKAYILDMFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFG
LPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFSYDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVN*CPELG
TVLANEELKREKNGALVSERGNFPVILKKMKQ*VLKITEYAQKLLDGLDQVDFPDSLKTLQKK*IGKSKG*KIKFLIEKT
GEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKNKEKISQFIEQNSFSSPKLQSKMTGIFTGLYAVHPLNSSKIPIYIT
NYVLNNYATSAIMGVPAHNLADLEFAQIFQIDYVKVINQENLLINSAEFSGLTIKEGANSIFSKLNSMNLVQKTTIFRLK
D*VFSRQRY*GEPFPVYFDEKGKIYLEEKIVELPHTDKIMPSGDGQSPLASIKD*VFFEKNGKYYRRETNTMPQ*SGSSW
YYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVGHLIYSRF*HKVLFDAGIVPNSEPFQKIIHQGMLLGSD
GQKMSKSKGNTINPTEIIDKFGADTIRIFLMFMGPIGDLRL*DENGVVSIFN*IQRVQRTILADYKIDPELEKDYEFTYF
FNNFVFEITNLIETFKFNVAISKLMVYINFLNKKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQI*PQFDR
SKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIEIAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=875, Percent_Identity=29.1428571428571, Blast_Score=308, Evalue=2e-83,
Organism=Escherichia coli, GI1786861, Length=877, Percent_Identity=32.9532497149373, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71997510, Length=730, Percent_Identity=28.0821917808219, Blast_Score=208, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI71997517, Length=730, Percent_Identity=28.0821917808219, Blast_Score=208, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI212645227, Length=278, Percent_Identity=25.8992805755396, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323414, Length=858, Percent_Identity=32.983682983683, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI21355409, Length=841, Percent_Identity=27.705112960761, Blast_Score=254, Evalue=2e-67,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 89527; Mature: 89527

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDHRAIEKKQDYWYKNKVFKTTETSRKKAYILDMFPYPSASGLHLGHPIGYTASDIVAR
CCCCHHHHHHHCCHHHCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
FKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFSYDWDKE
HHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
INTSDPEFYVQTQWIFKLLYKANLAEIREVEVNCPELGTVLANEELKREKNGALVSERGN
CCCCCCCEEEEHHHHHHHHHHCCHHHHHEEECCCCHHHHHHHCHHHHHHHCCCEEECCCC
FPVILKKMKQVLKITEYAQKLLDGLDQVDFPDSLKTLQKKIGKSKGKIKFLIEKTGEFLE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCHHHH
IFTTKIETFYGVSFLALAPLHNFAQKLAKNKEKISQFIEQNSFSSPKLQSKMTGIFTGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVHPLNSSKIPIYITNYVLNNYATSAIMGVPAHNLADLEFAQIFQIDYVKVINQENLLIN
HEECCCCCCCCEEEEHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHEEECCCCEEEE
SAEFSGLTIKEGANSIFSKLNSMNLVQKTTIFRLKDVFSRQRYGEPFPVYFDEKGKIYLE
CCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEE
EKIVELPHTDKIMPSGDGQSPLASIKDVFFEKNGKYYRRETNTMPQSGSSWYYLAFILKE
EHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEC
KDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVGHLIYSRFHKVLFDAGIVPNSEPFQKI
CCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IHQGMLLGSDGQKMSKSKGNTINPTEIIDKFGADTIRIFLMFMGPIGDLRLDENGVVSIF
HHCCEEECCCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEE
NIQRVQRTILADYKIDPELEKDYEFTYFFNNFVFEITNLIETFKFNVAISKLMVYINFLN
EHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQIPQFDRSKIFKNTFHLPVS
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEEE
INGKNRGILELTDEKSQEEIIEIAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN
ECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCC
>Mature Secondary Structure
MLDHRAIEKKQDYWYKNKVFKTTETSRKKAYILDMFPYPSASGLHLGHPIGYTASDIVAR
CCCCHHHHHHHCCHHHCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHH
FKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFSYDWDKE
HHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
INTSDPEFYVQTQWIFKLLYKANLAEIREVEVNCPELGTVLANEELKREKNGALVSERGN
CCCCCCCEEEEHHHHHHHHHHCCHHHHHEEECCCCHHHHHHHCHHHHHHHCCCEEECCCC
FPVILKKMKQVLKITEYAQKLLDGLDQVDFPDSLKTLQKKIGKSKGKIKFLIEKTGEFLE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCHHHH
IFTTKIETFYGVSFLALAPLHNFAQKLAKNKEKISQFIEQNSFSSPKLQSKMTGIFTGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVHPLNSSKIPIYITNYVLNNYATSAIMGVPAHNLADLEFAQIFQIDYVKVINQENLLIN
HEECCCCCCCCEEEEHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHEEECCCCEEEE
SAEFSGLTIKEGANSIFSKLNSMNLVQKTTIFRLKDVFSRQRYGEPFPVYFDEKGKIYLE
CCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEE
EKIVELPHTDKIMPSGDGQSPLASIKDVFFEKNGKYYRRETNTMPQSGSSWYYLAFILKE
EHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEC
KDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVGHLIYSRFHKVLFDAGIVPNSEPFQKI
CCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IHQGMLLGSDGQKMSKSKGNTINPTEIIDKFGADTIRIFLMFMGPIGDLRLDENGVVSIF
HHCCEEECCCCCHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEECCCCCEEEE
NIQRVQRTILADYKIDPELEKDYEFTYFFNNFVFEITNLIETFKFNVAISKLMVYINFLN
EHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQIPQFDRSKIFKNTFHLPVS
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEEE
INGKNRGILELTDEKSQEEIIEIAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN
ECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]