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The map label for this gene is dnaB [H]

Identifier: 71893992

GI number: 71893992

Start: 843656

End: 845047

Strand: Reverse

Name: dnaB [H]

Synonym: MHJ_0644

Alternate gene names: 71893992

Gene position: 845047-843656 (Counterclockwise)

Preceding gene: 71893993

Following gene: 71893991

Centisome position: 94.17

GC content: 30.1

Gene sequence:

>1392_bases
ATGAATAATTCTCGTTATAATTCTCCTGAACTTGAAGCATTTATCATCTCTTTTCTTTTAGCAAATCCTGAAAAAGTTGT
TAATTTTATTGACATACTTAATCCGGATGATTTCAGTGTTAGAAATTTAGCTGAAATTGTAAGAGCAATAAAAAATGTTG
CAAATCAAACAAAAAATCCTGAGGTTACCCTAGTTCTTCAGGAACTCGAAAGAACGGGTAAATTAGATCAAATCGGCGGG
CGCAACTACATTGTAAATTTATATAACAGCAATTTTTCGCTACCTTCGGAAATCACTGAACATTTAAGAATAATAAATGA
AAAAAAAACTTTAAGAGATCTAAAAAAAATTGTTGATGATTCAACAAAAGAACTAAATTCCGGAACAAAATTTTCTTATG
AGATAACCACAGAAATTCTTCAAAAAATAAATCTTCTGAATTTTAATACTAAAAATGAAACTTTTATTCCGATTTATGAG
ATTGCAGTTCAAATTTTCGCATATTTAACAGAATTACGTACTAGTCAAAATTTAATTAAAGGAATTTCGACCGGATATGA
GAATTTAGACTTATTAACTTCTGGATTTCAAAAAGGTGATCTAACAATTTTAGCAGCCCGGCCTTCAGTCGGAAAAACAG
CTTTTGCCCTTAATTTAGCTTGAAATATTTGTGAAAATGATAAATCTGTCCTATTTTTTGCACTTGAAATGTCTAATTCT
GATTTAGTTACAAGACTTCTTTCGCTTATTTCTGGGGTTGAATCTGGAAAATTTAAAATCCCAAAAAATTTAAGAGCGGA
AGATTTAAAAGCAATCGAAAAGGCAATTGAAACCCGAATTAAAAACTGTAAATTAATAATTAATGACTCTGGATCGATTA
ATATTGATGATATTTACTGACAAGTTCAAAAAAGATATCGAAATAACCTAAAATATGATTTAATTATTTTTGATTATTTA
CAACTAATTAATTCAGCTACAAGAAACCAAAATTATAACCGTCAAGTCGAGGTATCAATTATTTCAAGAAAATTAAAACA
ACTAGCCCGTGAGGTCGAAACTCCTATTTTAGCACTTTCACAACTTTCAAGAAATGTTGAAAGAAGAGAGGATAAAACCC
CAGTTCTATCGGATTTAAGGGAATCTGGAGCAATTGAACAAGATGCTGATATTGTCGCTTTTCTACATCGTGAGGATTAT
TATCACAAAAAAGAGGAGAAACCCGATCAAGAACAGGGCCCAAATACGAAACTTATAATTGCAAAACATAGAAATGGACC
GATTGGAACTTTATTTTTTGATTTTTTTCCTGAATTTGGCAAGTTTAGTCCTGCAATTCCGGAGTCTTCATATAATAAAA
AAGCAAATATTAATTTAGGATTGGATGAATAA

Upstream 100 bases:

>100_bases
GCAATCCAAACAAGTGGAATTTCCACTTTTGGCACTAGTTTTGTTGATATAAAATTAAGTTCTCAAACTATTGCTAAATT
AAAAATAAATATCACAAAAG

Downstream 100 bases:

>100_bases
TGGCCTTATATTTTGCTATTTTATCCGTTGTTTTAGTGATTTTATTCTTTATTTCGGCAATTTTTTCTGCAAGCGAGACT
GTATTTACTGCAACAAGTCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNNSRYNSPELEAFIISFLLANPEKVVNFIDILNPDDFSVRNLAEIVRAIKNVANQTKNPEVTLVLQELERTGKLDQIGG
RNYIVNLYNSNFSLPSEITEHLRIINEKKTLRDLKKIVDDSTKELNSGTKFSYEITTEILQKINLLNFNTKNETFIPIYE
IAVQIFAYLTELRTSQNLIKGISTGYENLDLLTSGFQKGDLTILAARPSVGKTAFALNLAWNICENDKSVLFFALEMSNS
DLVTRLLSLISGVESGKFKIPKNLRAEDLKAIEKAIETRIKNCKLIINDSGSINIDDIYWQVQKRYRNNLKYDLIIFDYL
QLINSATRNQNYNRQVEVSIISRKLKQLAREVETPILALSQLSRNVERREDKTPVLSDLRESGAIEQDADIVAFLHREDY
YHKKEEKPDQEQGPNTKLIIAKHRNGPIGTLFFDFFPEFGKFSPAIPESSYNKKANINLGLDE

Sequences:

>Translated_463_residues
MNNSRYNSPELEAFIISFLLANPEKVVNFIDILNPDDFSVRNLAEIVRAIKNVANQTKNPEVTLVLQELERTGKLDQIGG
RNYIVNLYNSNFSLPSEITEHLRIINEKKTLRDLKKIVDDSTKELNSGTKFSYEITTEILQKINLLNFNTKNETFIPIYE
IAVQIFAYLTELRTSQNLIKGISTGYENLDLLTSGFQKGDLTILAARPSVGKTAFALNLA*NICENDKSVLFFALEMSNS
DLVTRLLSLISGVESGKFKIPKNLRAEDLKAIEKAIETRIKNCKLIINDSGSINIDDIY*QVQKRYRNNLKYDLIIFDYL
QLINSATRNQNYNRQVEVSIISRKLKQLAREVETPILALSQLSRNVERREDKTPVLSDLRESGAIEQDADIVAFLHREDY
YHKKEEKPDQEQGPNTKLIIAKHRNGPIGTLFFDFFPEFGKFSPAIPESSYNKKANINLGLDE
>Mature_463_residues
MNNSRYNSPELEAFIISFLLANPEKVVNFIDILNPDDFSVRNLAEIVRAIKNVANQTKNPEVTLVLQELERTGKLDQIGG
RNYIVNLYNSNFSLPSEITEHLRIINEKKTLRDLKKIVDDSTKELNSGTKFSYEITTEILQKINLLNFNTKNETFIPIYE
IAVQIFAYLTELRTSQNLIKGISTGYENLDLLTSGFQKGDLTILAARPSVGKTAFALNLA*NICENDKSVLFFALEMSNS
DLVTRLLSLISGVESGKFKIPKNLRAEDLKAIEKAIETRIKNCKLIINDSGSINIDDIY*QVQKRYRNNLKYDLIIFDYL
QLINSATRNQNYNRQVEVSIISRKLKQLAREVETPILALSQLSRNVERREDKTPVLSDLRESGAIEQDADIVAFLHREDY
YHKKEEKPDQEQGPNTKLIIAKHRNGPIGTLFFDFFPEFGKFSPAIPESSYNKKANINLGLDE

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=441, Percent_Identity=30.8390022675737, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52529; Mature: 52529

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSRYNSPELEAFIISFLLANPEKVVNFIDILNPDDFSVRNLAEIVRAIKNVANQTKNP
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
EVTLVLQELERTGKLDQIGGRNYIVNLYNSNFSLPSEITEHLRIINEKKTLRDLKKIVDD
CEEEEEHHHHHCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STKELNSGTKFSYEITTEILQKINLLNFNTKNETFIPIYEIAVQIFAYLTELRTSQNLIK
CHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GISTGYENLDLLTSGFQKGDLTILAARPSVGKTAFALNLANICENDKSVLFFALEMSNSD
HHHCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEHHHHCCCCCCEEEEEEECCCCH
LVTRLLSLISGVESGKFKIPKNLRAEDLKAIEKAIETRIKNCKLIINDSGSINIDDIYQV
HHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
QKRYRNNLKYDLIIFDYLQLINSATRNQNYNRQVEVSIISRKLKQLAREVETPILALSQL
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNVERREDKTPVLSDLRESGAIEQDADIVAFLHREDYYHKKEEKPDQEQGPNTKLIIAK
HHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEE
HRNGPIGTLFFDFFPEFGKFSPAIPESSYNKKANINLGLDE
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure
MNNSRYNSPELEAFIISFLLANPEKVVNFIDILNPDDFSVRNLAEIVRAIKNVANQTKNP
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
EVTLVLQELERTGKLDQIGGRNYIVNLYNSNFSLPSEITEHLRIINEKKTLRDLKKIVDD
CEEEEEHHHHHCCCCHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STKELNSGTKFSYEITTEILQKINLLNFNTKNETFIPIYEIAVQIFAYLTELRTSQNLIK
CHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GISTGYENLDLLTSGFQKGDLTILAARPSVGKTAFALNLANICENDKSVLFFALEMSNSD
HHHCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHEEHHHHCCCCCCEEEEEEECCCCH
LVTRLLSLISGVESGKFKIPKNLRAEDLKAIEKAIETRIKNCKLIINDSGSINIDDIYQV
HHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHH
QKRYRNNLKYDLIIFDYLQLINSATRNQNYNRQVEVSIISRKLKQLAREVETPILALSQL
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
SRNVERREDKTPVLSDLRESGAIEQDADIVAFLHREDYYHKKEEKPDQEQGPNTKLIIAK
HHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCEEEEEE
HRNGPIGTLFFDFFPEFGKFSPAIPESSYNKKANINLGLDE
CCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]