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The map label for this gene is nusA [H]

Identifier: 71893935

GI number: 71893935

Start: 755910

End: 757763

Strand: Reverse

Name: nusA [H]

Synonym: MHJ_0586

Alternate gene names: 71893935

Gene position: 757763-755910 (Counterclockwise)

Preceding gene: 71893937

Following gene: 71893934

Centisome position: 84.44

GC content: 30.2

Gene sequence:

>1854_bases
ATTTCGAAAATGAATAGAAAAAAAACCGAAAAATCAAAAATTAGTTCAAAAGATAGTAAAAAATTAATAATTCAAGCTAT
CCAAGATGTGGCAAAAAATAGCGAATTAAATCTGGAAGCAGTTATTGATATTTTTCAGGAAGCAATTGAATTTGTAATAA
CAAAGAAAATTGACCCAGATGCGCAAATAAAAATTGAAGCTGATCTTGAACAATTGAGTTTTAAAGTTTTTAATACAAAC
GGGATTGTTGTTGAAGAAAATTATTTTGATGATCTTACAGATGAGGAAAAAGTTAACGATCTTGTTTCTTTTATTTTACT
ATCAAAGGCAAAAGAAACTGATCCTGAGATTCAGGTTGATGATATTTTTTCAATTGAAATTAATCTTGAAAGTTTTGAAC
ATTGACTTTTTATGGCAATTATGCACGCTTTTAAGCAAAAAATTTCTGAAATTGTCCGAAATAATGTTTATAACAAATAT
TTATCGCTTAAAAATAACGTAGTTTTGGCCACTGTTACTAATAAAATTGCTGCTGGTTATATTTTCGAAATTGACGATGA
TAAAGTTTCTGCCTTTATGCCAAGCCATTATGCAAGTGGACAAAATTTAAAAATAGGCACTAAACATGAAGTTGTAATTG
AAAATGTATCAAAAAATACAAAACAGTCGCAGGTTGTGATATCCTCAAAATCAGTTCAACTTGTCAAGAAAAAAATAATC
GATGCAATCCCTGAACTACAGTCAAAATTTCTTGAAATCACTTCAATTGCACGGATTCCAGGGGAAAGATGTAAAGTCGC
AATTCGCAGAAATGAGGATGCCGAAGCTGATAATATTTCTGAAATCGGCTCAATTGTAGGAGCAACTGGCTCAAGAGTTC
TTGCAATTTCTCAAGAGCTTCAGGGTGAAAAAATTGAGGTGATTAAATATGATGATAATATTGTCAAATTTATTGTTAAT
GCGATGTCGCCTTCAAAAGTTATTTGCGTAAAAGAGTTCAAAATAGGTCATAAATTACGTCGTTTTATCGTAGTTGTTCC
TGATTTTCAACATAGTTTAGCCATTGGAAAAAACGGTTCAAATGTTAAACTAGTAGCAGATCTAACACGTTGTCAAGTGC
AAATTATCCCGTATTCAAGCGCGCTAAAAGATCAAAATTTTAAAATTGAATGAAATGGAAATATTAAAGACATTCAAGAA
CTAAACAGTCTTAAAAATGAGTATATTCATCGCCAGCAGGGTAGAATTTATCAAAATCATCGGAATTCATATGGTCAAGG
TAATAATAATTTTGACTTAATTTTACAGCAATTCGAATCTGATATTCGCGAGTTAGAAAAACCTTATGGGATTGAAAACG
AGTTTATACCAAAAAATGAGCAAAAACAGGTAAGAAGTCACCAAGAATTTCCAAAAAACAAGAATAATCTAGCAAAAGCA
GCAACTAAATCGCGTAATTTTAACAAAAGCCAGAATATCCGCGAAAATATTTCAAAAGATTTTGATTATGGCTTTGAAAA
CGAGAAAGATTCTAATTCTTTCCAAAATATTAGTCAAAGATCTTTTTTTGATGCAGATTCACTTTTTGATTCGGCTCTAA
ATGAGGCAATTAGTGAAAACGAGTTAATCGATAAAATTCACCAAGAAGAGGAAAAAAAACAAGAATTATTATTACAAGAG
AAGGAAAAATGAGCAAAAAATGAAGCTCAAATCCAAAAAGCTAGTCAAGAACCTAACCAAAATTCAGATTTATATATCCA
AAATGAAAAACAAATAAAAAATTTCAAATTAGATGATGATCTTCTAAAATATACAGGGGTTGACGATATTGACATTGATG
ATTTTGATTTCTAG

Upstream 100 bases:

>100_bases
AAACAAGTTGAGGAAATTTCAAAAGAAATTTCCATTTTTATCGATAAAATTGAGACCGATGAGAAAAATTTTATTTTAGA
AGTACTTTCAAAAGGGCAAG

Downstream 100 bases:

>100_bases
GATAAAAAAGTGAAAAATTATACAAGAAAATGTATTGTTGATCAAAAAATTTATCCAGTAAGTGAATTAATAAGATTTGT
TTATATTGATAAAAAGTTAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MSKMNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTN
GIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEHWLFMAIMHAFKQKISEIVRNNVYNKY
LSLKNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKII
DAIPELQSKFLEITSIARIPGERCKVAIRRNEDAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVN
AMSPSKVICVKEFKIGHKLRRFIVVVPDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIEWNGNIKDIQE
LNSLKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDIRELEKPYGIENEFIPKNEQKQVRSHQEFPKNKNNLAKA
ATKSRNFNKSQNIRENISKDFDYGFENEKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQE
KEKWAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF

Sequences:

>Translated_617_residues
MSKMNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTN
GIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEH*LFMAIMHAFKQKISEIVRNNVYNKY
LSLKNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKII
DAIPELQSKFLEITSIARIPGERCKVAIRRNEDAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVN
AMSPSKVICVKEFKIGHKLRRFIVVVPDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIE*NGNIKDIQE
LNSLKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDIRELEKPYGIENEFIPKNEQKQVRSHQEFPKNKNNLAKA
ATKSRNFNKSQNIRENISKDFDYGFENEKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQE
KEK*AKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF
>Mature_616_residues
SKMNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPDAQIKIEADLEQLSFKVFNTNG
IVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVDDIFSIEINLESFEH*LFMAIMHAFKQKISEIVRNNVYNKYL
SLKNNVVLATVTNKIAAGYIFEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIID
AIPELQSKFLEITSIARIPGERCKVAIRRNEDAEADNISEIGSIVGATGSRVLAISQELQGEKIEVIKYDDNIVKFIVNA
MSPSKVICVKEFKIGHKLRRFIVVVPDFQHSLAIGKNGSNVKLVADLTRCQVQIIPYSSALKDQNFKIE*NGNIKDIQEL
NSLKNEYIHRQQGRIYQNHRNSYGQGNNNFDLILQQFESDIRELEKPYGIENEFIPKNEQKQVRSHQEFPKNKNNLAKAA
TKSRNFNKSQNIRENISKDFDYGFENEKDSNSFQNISQRSFFDADSLFDSALNEAISENELIDKIHQEEEKKQELLLQEK
EK*AKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLKYTGVDDIDIDDFDF

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=358, Percent_Identity=23.1843575418994, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 70258; Mature: 70127

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPD
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQIKIEADLEQLSFKVFNTNGIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVD
CEEEEEECHHHCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEC
DIFSIEINLESFEHLFMAIMHAFKQKISEIVRNNVYNKYLSLKNNVVLATVTNKIAAGYI
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEE
FEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIID
EEECCCCHHHHCCHHCCCCCCEECCCCCHHHEECCCCCCCCCEEEEECHHHHHHHHHHHH
AIPELQSKFLEITSIARIPGERCKVAIRRNEDAEADNISEIGSIVGATGSRVLAISQELQ
HHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEHHCC
GEKIEVIKYDDNIVKFIVNAMSPSKVICVKEFKIGHKLRRFIVVVPDFQHSLAIGKNGSN
CCEEEEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHEEEEEEEECCCCCCEEECCCCCC
VKLVADLTRCQVQIIPYSSALKDQNFKIENGNIKDIQELNSLKNEYIHRQQGRIYQNHRN
EEEEEEECCCEEEEEECHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCC
SYGQGNNNFDLILQQFESDIRELEKPYGIENEFIPKNEQKQVRSHQEFPKNKNNLAKAAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
KSRNFNKSQNIRENISKDFDYGFENEKDSNSFQNISQRSFFDADSLFDSALNEAISENEL
HCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IDKIHQEEEKKQELLLQEKEKAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCHHHHH
YTGVDDIDIDDFDF
HCCCCCCCCCCCCC
>Mature Secondary Structure 
SKMNRKKTEKSKISSKDSKKLIIQAIQDVAKNSELNLEAVIDIFQEAIEFVITKKIDPD
CCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQIKIEADLEQLSFKVFNTNGIVVEENYFDDLTDEEKVNDLVSFILLSKAKETDPEIQVD
CEEEEEECHHHCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEC
DIFSIEINLESFEHLFMAIMHAFKQKISEIVRNNVYNKYLSLKNNVVLATVTNKIAAGYI
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCEE
FEIDDDKVSAFMPSHYASGQNLKIGTKHEVVIENVSKNTKQSQVVISSKSVQLVKKKIID
EEECCCCHHHHCCHHCCCCCCEECCCCCHHHEECCCCCCCCCEEEEECHHHHHHHHHHHH
AIPELQSKFLEITSIARIPGERCKVAIRRNEDAEADNISEIGSIVGATGSRVLAISQELQ
HHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEEHHCC
GEKIEVIKYDDNIVKFIVNAMSPSKVICVKEFKIGHKLRRFIVVVPDFQHSLAIGKNGSN
CCEEEEEEECCHHHHHHHHHCCCCEEEEEHHHHHHHHEEEEEEEECCCCCCEEECCCCCC
VKLVADLTRCQVQIIPYSSALKDQNFKIENGNIKDIQELNSLKNEYIHRQQGRIYQNHRN
EEEEEEECCCEEEEEECHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCC
SYGQGNNNFDLILQQFESDIRELEKPYGIENEFIPKNEQKQVRSHQEFPKNKNNLAKAAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
KSRNFNKSQNIRENISKDFDYGFENEKDSNSFQNISQRSFFDADSLFDSALNEAISENEL
HCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
IDKIHQEEEKKQELLLQEKEKAKNEAQIQKASQEPNQNSDLYIQNEKQIKNFKLDDDLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCHHHHH
YTGVDDIDIDDFDF
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]