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The map label for this gene is infB [H]

Identifier: 71893934

GI number: 71893934

Start: 753844

End: 755643

Strand: Reverse

Name: infB [H]

Synonym: MHJ_0585

Alternate gene names: 71893934

Gene position: 755643-753844 (Counterclockwise)

Preceding gene: 71893935

Following gene: 71893933

Centisome position: 84.2

GC content: 35.0

Gene sequence:

>1800_bases
ATGAAAAAAAGTCAAAAACGAATCTCAAATGTAAGCGAAATTAAAGCACAATTAAAAACTGTTGAAACTAAAGTTCATAA
CGGCGTCTTCCTTTTTTCAGGAATAATGACAATTGCTGAATTAGCTCAAAAAATTAATGTCTCAGTGAATCAAATTATTA
CTTATTTTTTCCATCAGGCAAAAATGTATAATTTAAATCATAGTCTTTCTGAAGACGAAATTGCCGAAATATGCCTTGAA
TTTGGGCTTGATTTTAAAAAAGAAGTCCAAATTGATGCCTCTAATTTTATGGAGGAAGTTTCAATTCTTGATCAGGACAA
AGATCTTAGCCCCCGTCCGCCAATCATCACAGTTATGGGTCATGTTGACCATGGAAAGACAACCTTACTTGATTATATCC
GGAAAACTAATATTGCAAAAAATGAAAAAGGTGGAATTACCCAGCATACTGGTGCTTATCAAGTTGTTTTTCAAGGTCAT
ATTATTAATTTTATTGATACTCCTGGTCATGAAGCATTTACACAAATGCGCGCCCGCGGGGCAAAAGTGACCGATATTAT
CGTCTTAGTTGTTGCTGCTGATGATGGAGTGATGCCTCAGACAAAAGAAGCAATTAACCATGCTGCAGCCGCAAATGTGC
CAATAATCGTTTTTGTTAATAAAATGGATAAGCCAAACAAGGATGTTGATAGGATCAAAAATGAACTATCTGCGCTTAAT
ATTGTCACCGAAGAATGAGGTGGAAGTAACATTTTTGTCTATGGATCGGCACTAACGGGTCAAGGAATAGATACCCTTTT
TAGTTCAATTTTACTACTTGCAGAAATTTTAGAATTAAAAGCAAATAAAAATCGCTATCCAATCGGGACAGTTATTGAGG
CAAAATTGCACCATAATAAAGGGACAATTGCAACTTTAATGGTTCAAAATGGGACTTTAATGGTCAGAGATTTTATCGTT
GCTGGTTATCAATATGGTCGGATTCGTTCACTGGAAAATACCAATGGTCAGCCAATCAAATTTGCTCCCCCGGGAACTCC
GGTAATTGTCACTGGGCTTAATTATGTCCCGGAAGCCGGGGATAAATTTTTCGGATTTCACGAAGAAAAATTTGCAAAAC
AATTAGCTCTTGAAAGAAAACAGTCAGAGAAAATTTCAAAGACAAAAGTGCAAACAAAACAGCAAACAAAAGAGAAAACA
CTAAATATAATTATCAAGGCCGATGTTGCCGGAATTGCCCAGGCTTTGCATTCAACAATTGAAAAACTTGCATCAAAACA
AGTGCATATTCACATTTTGCACTCTGGTGTCGGCATTGTAAATAAGGCAGATATTTTACTTGCCCAAACTTCAAATTCAA
TAATTTATGCCTTTAATTTACAAATTCCAGCTGCAATTAAAGCCCAAGCAAAACAAGCCCAAGTCGAAATTCGCGAACAT
ACAATTATTTATAAAATTGTTGATGAAATTAAAAAACAAGTCCGTGGAATGCGTGAGATTAGATATGAACTTCAGCAGAT
CGGGACAGCAAAAATTATTGCTAAATTCTGATTTTCAAAAGTCGGATCAATTGCAGGATGTAGTGTACTTTCGGGAAAAT
TTGTCGAAAATTGTAAAATTGAGCTTTGAAGAAATTCAAAACTGATCCATTCAGGAAAAATTGAATCCCTACAAAGAGAT
AAAAATCCGGTAAAAGAAGTTCAGGTCGGCAACGAGTTTGGAACTCATATCTATAAATTTAATGATATTGAAATAGGTGA
TGAGCTAAAAGCATTTTTAGATGTTGAAATTGAGGAATAA

Upstream 100 bases:

>100_bases
AAATTCAACAAGCAATTCAAAAAAAACTTTTTAATCGTGCATTTAAGACAAATATTTCCACTCTTGCCTATACAAATTTA
GCAAATGAGGTAGAAATTCT

Downstream 100 bases:

>100_bases
AAAGGAAGAGAATGTCTGTTAGTCACGAGAAAAAACAAAATTATTACTTTCAAATTATTTCAAAAGTAATCGAGACATAT
TTTCTAGAAAGGATGCCGGT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKKSQKRISNVSEIKAQLKTVETKVHNGVFLFSGIMTIAELAQKINVSVNQIITYFFHQAKMYNLNHSLSEDEIAEICLE
FGLDFKKEVQIDASNFMEEVSILDQDKDLSPRPPIITVMGHVDHGKTTLLDYIRKTNIAKNEKGGITQHTGAYQVVFQGH
IINFIDTPGHEAFTQMRARGAKVTDIIVLVVAADDGVMPQTKEAINHAAAANVPIIVFVNKMDKPNKDVDRIKNELSALN
IVTEEWGGSNIFVYGSALTGQGIDTLFSSILLLAEILELKANKNRYPIGTVIEAKLHHNKGTIATLMVQNGTLMVRDFIV
AGYQYGRIRSLENTNGQPIKFAPPGTPVIVTGLNYVPEAGDKFFGFHEEKFAKQLALERKQSEKISKTKVQTKQQTKEKT
LNIIIKADVAGIAQALHSTIEKLASKQVHIHILHSGVGIVNKADILLAQTSNSIIYAFNLQIPAAIKAQAKQAQVEIREH
TIIYKIVDEIKKQVRGMREIRYELQQIGTAKIIAKFWFSKVGSIAGCSVLSGKFVENCKIELWRNSKLIHSGKIESLQRD
KNPVKEVQVGNEFGTHIYKFNDIEIGDELKAFLDVEIEE

Sequences:

>Translated_599_residues
MKKSQKRISNVSEIKAQLKTVETKVHNGVFLFSGIMTIAELAQKINVSVNQIITYFFHQAKMYNLNHSLSEDEIAEICLE
FGLDFKKEVQIDASNFMEEVSILDQDKDLSPRPPIITVMGHVDHGKTTLLDYIRKTNIAKNEKGGITQHTGAYQVVFQGH
IINFIDTPGHEAFTQMRARGAKVTDIIVLVVAADDGVMPQTKEAINHAAAANVPIIVFVNKMDKPNKDVDRIKNELSALN
IVTEE*GGSNIFVYGSALTGQGIDTLFSSILLLAEILELKANKNRYPIGTVIEAKLHHNKGTIATLMVQNGTLMVRDFIV
AGYQYGRIRSLENTNGQPIKFAPPGTPVIVTGLNYVPEAGDKFFGFHEEKFAKQLALERKQSEKISKTKVQTKQQTKEKT
LNIIIKADVAGIAQALHSTIEKLASKQVHIHILHSGVGIVNKADILLAQTSNSIIYAFNLQIPAAIKAQAKQAQVEIREH
TIIYKIVDEIKKQVRGMREIRYELQQIGTAKIIAKF*FSKVGSIAGCSVLSGKFVENCKIEL*RNSKLIHSGKIESLQRD
KNPVKEVQVGNEFGTHIYKFNDIEIGDELKAFLDVEIEE
>Mature_599_residues
MKKSQKRISNVSEIKAQLKTVETKVHNGVFLFSGIMTIAELAQKINVSVNQIITYFFHQAKMYNLNHSLSEDEIAEICLE
FGLDFKKEVQIDASNFMEEVSILDQDKDLSPRPPIITVMGHVDHGKTTLLDYIRKTNIAKNEKGGITQHTGAYQVVFQGH
IINFIDTPGHEAFTQMRARGAKVTDIIVLVVAADDGVMPQTKEAINHAAAANVPIIVFVNKMDKPNKDVDRIKNELSALN
IVTEE*GGSNIFVYGSALTGQGIDTLFSSILLLAEILELKANKNRYPIGTVIEAKLHHNKGTIATLMVQNGTLMVRDFIV
AGYQYGRIRSLENTNGQPIKFAPPGTPVIVTGLNYVPEAGDKFFGFHEEKFAKQLALERKQSEKISKTKVQTKQQTKEKT
LNIIIKADVAGIAQALHSTIEKLASKQVHIHILHSGVGIVNKADILLAQTSNSIIYAFNLQIPAAIKAQAKQAQVEIREH
TIIYKIVDEIKKQVRGMREIRYELQQIGTAKIIAKF*FSKVGSIAGCSVLSGKFVENCKIEL*RNSKLIHSGKIESLQRD
KNPVKEVQVGNEFGTHIYKFNDIEIGDELKAFLDVEIEE

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=543, Percent_Identity=36.8324125230203, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI53729337, Length=543, Percent_Identity=36.8324125230203, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI84043963, Length=263, Percent_Identity=32.6996197718631, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI94966754, Length=156, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=35.5555555555556, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI19923640, Length=135, Percent_Identity=35.5555555555556, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI25306283, Length=135, Percent_Identity=35.5555555555556, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI157426893, Length=246, Percent_Identity=26.4227642276423, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1789559, Length=577, Percent_Identity=42.1143847487002, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI2367247, Length=239, Percent_Identity=32.2175732217573, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994988, Length=169, Percent_Identity=33.1360946745562, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1790412, Length=126, Percent_Identity=38.0952380952381, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789737, Length=126, Percent_Identity=38.0952380952381, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=607, Percent_Identity=30.6425041186161, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI212656558, Length=269, Percent_Identity=32.3420074349442, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=218, Percent_Identity=29.3577981651376, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556745, Length=111, Percent_Identity=39.6396396396396, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=35.3741496598639, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324550, Length=641, Percent_Identity=30.7332293291732, Blast_Score=282, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319282, Length=136, Percent_Identity=38.9705882352941, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324761, Length=162, Percent_Identity=33.9506172839506, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI28572034, Length=620, Percent_Identity=33.2258064516129, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI160714833, Length=73, Percent_Identity=63.013698630137, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656849, Length=131, Percent_Identity=38.9312977099237, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 66206; Mature: 66206

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSQKRISNVSEIKAQLKTVETKVHNGVFLFSGIMTIAELAQKINVSVNQIITYFFHQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KMYNLNHSLSEDEIAEICLEFGLDFKKEVQIDASNFMEEVSILDQDKDLSPRPPIITVMG
HEECCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEE
HVDHGKTTLLDYIRKTNIAKNEKGGITQHTGAYQVVFQGHIINFIDTPGHEAFTQMRARG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHCC
AKVTDIIVLVVAADDGVMPQTKEAINHAAAANVPIIVFVNKMDKPNKDVDRIKNELSALN
CEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHEE
IVTEEGGSNIFVYGSALTGQGIDTLFSSILLLAEILELKANKNRYPIGTVIEAKLHHNKG
EEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
TIATLMVQNGTLMVRDFIVAGYQYGRIRSLENTNGQPIKFAPPGTPVIVTGLNYVPEAGD
CEEEEEEECCEEEEEHHHHHCCCCCCEEECCCCCCCEEEECCCCCCEEEECCCCCCCCCC
KFFGFHEEKFAKQLALERKQSEKISKTKVQTKQQTKEKTLNIIIKADVAGIAQALHSTIE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
KLASKQVHIHILHSGVGIVNKADILLAQTSNSIIYAFNLQIPAAIKAQAKQAQVEIREHT
HHCCCCEEEEEEECCCCCEECCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
IIYKIVDEIKKQVRGMREIRYELQQIGTAKIIAKFFSKVGSIAGCSVLSGKFVENCKIEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCHHHCCEEEE
RNSKLIHSGKIESLQRDKNPVKEVQVGNEFGTHIYKFNDIEIGDELKAFLDVEIEE
CCCEEEECCCHHHHHHCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHEEEEEEECC
>Mature Secondary Structure
MKKSQKRISNVSEIKAQLKTVETKVHNGVFLFSGIMTIAELAQKINVSVNQIITYFFHQA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KMYNLNHSLSEDEIAEICLEFGLDFKKEVQIDASNFMEEVSILDQDKDLSPRPPIITVMG
HEECCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEE
HVDHGKTTLLDYIRKTNIAKNEKGGITQHTGAYQVVFQGHIINFIDTPGHEAFTQMRARG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHCC
AKVTDIIVLVVAADDGVMPQTKEAINHAAAANVPIIVFVNKMDKPNKDVDRIKNELSALN
CEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHEE
IVTEEGGSNIFVYGSALTGQGIDTLFSSILLLAEILELKANKNRYPIGTVIEAKLHHNKG
EEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
TIATLMVQNGTLMVRDFIVAGYQYGRIRSLENTNGQPIKFAPPGTPVIVTGLNYVPEAGD
CEEEEEEECCEEEEEHHHHHCCCCCCEEECCCCCCCEEEECCCCCCEEEECCCCCCCCCC
KFFGFHEEKFAKQLALERKQSEKISKTKVQTKQQTKEKTLNIIIKADVAGIAQALHSTIE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
KLASKQVHIHILHSGVGIVNKADILLAQTSNSIIYAFNLQIPAAIKAQAKQAQVEIREHT
HHCCCCEEEEEEECCCCCEECCEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
IIYKIVDEIKKQVRGMREIRYELQQIGTAKIIAKFFSKVGSIAGCSVLSGKFVENCKIEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCHHHCCEEEE
RNSKLIHSGKIESLQRDKNPVKEVQVGNEFGTHIYKFNDIEIGDELKAFLDVEIEE
CCCEEEECCCHHHHHHCCCCHHHHCCCCCCCCEEEEECCCCCCHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA