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The map label for this gene is tilS

Identifier: 71892070

GI number: 71892070

Start: 344776

End: 346308

Strand: Direct

Name: tilS

Synonym: BPEN_296

Alternate gene names: 71892070

Gene position: 344776-346308 (Clockwise)

Preceding gene: 71892069

Following gene: 71892072

Centisome position: 43.55

GC content: 30.01

Gene sequence:

>1533_bases
ATGATCACAACCAATACATCGTCAGAAAATTGGGATTTATATCGTAAAGTAACACATTGTCTTGTCGGACATACAAGATT
AGTGTTGTCTTATAGTGGAGGACTGGATTCCACAGTACTTTTAGATATTTTAACAAGATTAAAAAATAATTCTGATCATT
TCACATCATCTCCGTTCATGTTGCGCGCAATCTATGTACACCATGGTATTAGTAATTATTCGGATGAATGGGCTAGTCAT
TGTTTTAATCAATGTAAAATACGGGGCATACCTTTTTCAGTAATTCATATCAATTGCTATAATGCAGAAAATAAACAACG
CAATATAGAAGCATTAGCACGAAATCTTCGTTATAAAAAATTATACAATCGTTTAAATTCAAAAGAAATACTTTTGACCG
CGCATCACATGAATGATCAAGTAGAAAGTTTATTCCTTGCACTAAAGAGAGGGAGTGGTCCATCTGGACTATCTGGAATG
AGTAAAAATGCATTATATGCTAATAAATACAGGTTATTAAGACCTTTATTGGATTGCTCTCGTGAACAATTAGAAATGTA
TGCTTATAAAAAAAAACTTGTTTGGATAGAAGACGATACGAATACAGATACACGTTTTGATCGTAATTTTTTACGCATAA
AAATTTTGCCATCAATATATCAACGTTGGCCTTGTTTTAATCAAGTAGTTGCGCGTACTGCGCAACTATGCAGAGATCAA
GAAAATTTATTAAATGAATTGTTGTCAGAATCTTTTCAAAAATTAATTGATGAATCAGATGGTTCTTTACTATTTATCCC
ATTATTTCAATACAGCATACCAAAAAGACAAGCTTTACTACGTCGTTGGCTATTGCATTTTTCTATGAAGATGCCTTCCT
ATCAACTTATAAATCGTATTTGGAAAGAAGTAGTATTAAGTCGGAAAGATGCAACCCCAATACTACAATTAGATAAATAC
CTATGTCGTCGTTTTCGTGAAAAGTTGTATATTTTACCTGTAAATATGAGATGTTCTTTAAATAGAATTGAATTATCATG
GAACATCATACATAATGTATTTATACTACCATATAATTTAGGTAAATTAATATACCAACCATTAAGTATTAACGAACATG
TTCCTAAAAATCCATTTGATCTGCATTTAAACATAAATTCTTCATTAAACACGTCTCATGATGTTTTTGAAAAATATAAA
AAAGTTTTAACGCATTGTTTTGTTCGATCCCCAAAATCTGATGAAAAAATATCAATTATTTTTGGAAATATAGATGGGTT
ATTATATATTTTAGGAAGAAATCATGGTCGACATTTAAAAAAAATTTGGCAGGAATTAGGCGTACCTCCATGGCTAAGAA
GTCGTATTCCTTTGCTTTTTTACAATAAAACATTAATTACTGCCATAGGAGTATTCATTACTCACAATGGCAAAATTATA
AGTAAAGAGAATACGTTATGGAAAATATCTTGGTTACAAGATATTATATCTTATAAAATATTTAAAAATAGCGTTCGTTA
TCATTTAGAATGA

Upstream 100 bases:

>100_bases
AATAATGTAAGTTCATTAATAATATATTAAATATTAATATAATCATTATTATTTAATATTTAAATCGTCATGACTTTATA
TTTACTACATTAGTAAAAAT

Downstream 100 bases:

>100_bases
TTTTTCATATCTATTAACTAATTAAATAAAATAATTAGCCCTAAAACTATAAGTTTTACAAATTGGTTTATTTATTTAAT
AAATAATAGCTATTTGATAT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MITTNTSSENWDLYRKVTHCLVGHTRLVLSYSGGLDSTVLLDILTRLKNNSDHFTSSPFMLRAIYVHHGISNYSDEWASH
CFNQCKIRGIPFSVIHINCYNAENKQRNIEALARNLRYKKLYNRLNSKEILLTAHHMNDQVESLFLALKRGSGPSGLSGM
SKNALYANKYRLLRPLLDCSREQLEMYAYKKKLVWIEDDTNTDTRFDRNFLRIKILPSIYQRWPCFNQVVARTAQLCRDQ
ENLLNELLSESFQKLIDESDGSLLFIPLFQYSIPKRQALLRRWLLHFSMKMPSYQLINRIWKEVVLSRKDATPILQLDKY
LCRRFREKLYILPVNMRCSLNRIELSWNIIHNVFILPYNLGKLIYQPLSINEHVPKNPFDLHLNINSSLNTSHDVFEKYK
KVLTHCFVRSPKSDEKISIIFGNIDGLLYILGRNHGRHLKKIWQELGVPPWLRSRIPLLFYNKTLITAIGVFITHNGKII
SKENTLWKISWLQDIISYKIFKNSVRYHLE

Sequences:

>Translated_510_residues
MITTNTSSENWDLYRKVTHCLVGHTRLVLSYSGGLDSTVLLDILTRLKNNSDHFTSSPFMLRAIYVHHGISNYSDEWASH
CFNQCKIRGIPFSVIHINCYNAENKQRNIEALARNLRYKKLYNRLNSKEILLTAHHMNDQVESLFLALKRGSGPSGLSGM
SKNALYANKYRLLRPLLDCSREQLEMYAYKKKLVWIEDDTNTDTRFDRNFLRIKILPSIYQRWPCFNQVVARTAQLCRDQ
ENLLNELLSESFQKLIDESDGSLLFIPLFQYSIPKRQALLRRWLLHFSMKMPSYQLINRIWKEVVLSRKDATPILQLDKY
LCRRFREKLYILPVNMRCSLNRIELSWNIIHNVFILPYNLGKLIYQPLSINEHVPKNPFDLHLNINSSLNTSHDVFEKYK
KVLTHCFVRSPKSDEKISIIFGNIDGLLYILGRNHGRHLKKIWQELGVPPWLRSRIPLLFYNKTLITAIGVFITHNGKII
SKENTLWKISWLQDIISYKIFKNSVRYHLE
>Mature_510_residues
MITTNTSSENWDLYRKVTHCLVGHTRLVLSYSGGLDSTVLLDILTRLKNNSDHFTSSPFMLRAIYVHHGISNYSDEWASH
CFNQCKIRGIPFSVIHINCYNAENKQRNIEALARNLRYKKLYNRLNSKEILLTAHHMNDQVESLFLALKRGSGPSGLSGM
SKNALYANKYRLLRPLLDCSREQLEMYAYKKKLVWIEDDTNTDTRFDRNFLRIKILPSIYQRWPCFNQVVARTAQLCRDQ
ENLLNELLSESFQKLIDESDGSLLFIPLFQYSIPKRQALLRRWLLHFSMKMPSYQLINRIWKEVVLSRKDATPILQLDKY
LCRRFREKLYILPVNMRCSLNRIELSWNIIHNVFILPYNLGKLIYQPLSINEHVPKNPFDLHLNINSSLNTSHDVFEKYK
KVLTHCFVRSPKSDEKISIIFGNIDGLLYILGRNHGRHLKKIWQELGVPPWLRSRIPLLFYNKTLITAIGVFITHNGKII
SKENTLWKISWLQDIISYKIFKNSVRYHLE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=466, Percent_Identity=39.2703862660944, Blast_Score=322, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_BLOPB (Q493B5)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277800.1
- ProteinModelPortal:   Q493B5
- SMR:   Q493B5
- STRING:   Q493B5
- GeneID:   3562841
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_296
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   PLLFYND
- ProtClustDB:   CLSK435157
- BioCyc:   CBLO291272:BPEN_296-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 60035; Mature: 60035

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITTNTSSENWDLYRKVTHCLVGHTRLVLSYSGGLDSTVLLDILTRLKNNSDHFTSSPFM
CCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHH
LRAIYVHHGISNYSDEWASHCFNQCKIRGIPFSVIHINCYNAENKQRNIEALARNLRYKK
HHHHHHHCCCCCCHHHHHHHHHHHCEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
LYNRLNSKEILLTAHHMNDQVESLFLALKRGSGPSGLSGMSKNALYANKYRLLRPLLDCS
HHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
REQLEMYAYKKKLVWIEDDTNTDTRFDRNFLRIKILPSIYQRWPCFNQVVARTAQLCRDQ
HHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHCCH
ENLLNELLSESFQKLIDESDGSLLFIPLFQYSIPKRQALLRRWLLHFSMKMPSYQLINRI
HHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
WKEVVLSRKDATPILQLDKYLCRRFREKLYILPVNMRCSLNRIELSWNIIHNVFILPYNL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEECCEEEEEEEEEEEEEEEEEECH
GKLIYQPLSINEHVPKNPFDLHLNINSSLNTSHDVFEKYKKVLTHCFVRSPKSDEKISII
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FGNIDGLLYILGRNHGRHLKKIWQELGVPPWLRSRIPLLFYNKTLITAIGVFITHNGKII
EECCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCEEEECHHHHHHHHHHEECCCEEE
SKENTLWKISWLQDIISYKIFKNSVRYHLE
ECCCCEEEHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MITTNTSSENWDLYRKVTHCLVGHTRLVLSYSGGLDSTVLLDILTRLKNNSDHFTSSPFM
CCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHH
LRAIYVHHGISNYSDEWASHCFNQCKIRGIPFSVIHINCYNAENKQRNIEALARNLRYKK
HHHHHHHCCCCCCHHHHHHHHHHHCEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHH
LYNRLNSKEILLTAHHMNDQVESLFLALKRGSGPSGLSGMSKNALYANKYRLLRPLLDCS
HHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
REQLEMYAYKKKLVWIEDDTNTDTRFDRNFLRIKILPSIYQRWPCFNQVVARTAQLCRDQ
HHHHHHHHHHEEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHCCH
ENLLNELLSESFQKLIDESDGSLLFIPLFQYSIPKRQALLRRWLLHFSMKMPSYQLINRI
HHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
WKEVVLSRKDATPILQLDKYLCRRFREKLYILPVNMRCSLNRIELSWNIIHNVFILPYNL
HHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEEECCEECCEEEEEEEEEEEEEEEEEECH
GKLIYQPLSINEHVPKNPFDLHLNINSSLNTSHDVFEKYKKVLTHCFVRSPKSDEKISII
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FGNIDGLLYILGRNHGRHLKKIWQELGVPPWLRSRIPLLFYNKTLITAIGVFITHNGKII
EECCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCEEEECHHHHHHHHHHEECCCEEE
SKENTLWKISWLQDIISYKIFKNSVRYHLE
ECCCCEEEHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA