Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 71892044

GI number: 71892044

Start: 307702

End: 309231

Strand: Direct

Name: lysS

Synonym: BPEN_270

Alternate gene names: 71892044

Gene position: 307702-309231 (Clockwise)

Preceding gene: 71892043

Following gene: 71892045

Centisome position: 38.87

GC content: 30.33

Gene sequence:

>1530_bases
ATGACTAAATACGTATATTCAAAGGATCAGTCATATCAAAAATTTAATGTCGATGATGAATTAAATATTCGTCGAAAAAA
ACTATCAAAACTCCGTGAAAAAGGCGTAGCATTTCCAAATAATTTTCGTCGTAATTCTATTTCTAATCAATTACATAAAA
AATACGCTCATACATCCAATGTAGAATTGATACAATTAAATATTGAAGTAACTATAGCAGGCCGTATAATAAGTCAACGT
ATCATGGGGAAAGCATCTTTTATAACTATACGCGATGCTGAAGGTTGCATACAATTGTATATTACTTCTAATTCATTAGC
AACAAATTTATATGATGAAAATATAAAACAATGGGATTTAGGAGATATCTTAGGAGCACGCGGTATATTGTTTAGAACGC
GTACCGGGGAACTATCAATATACTGTAAAGAAATTCGATTATTAACTAAATCATTGCGCCCATTACCAGATAAGTTTCAT
GGTTTAAATAATCAAGAAACAAAATATCGTCAAAGATATTTAGATTTAATTATTAATGAAAATACAAGAAAAACATTTAA
AATACGTTCTCTAGTGATCTCTGAAATACGTCAATTCATGAAAAAAAATAATTTCATGGAAGTAGAGACACCAATGATGC
ACACAATTGCAGGAGGAGCTATAGCGCATCCTTTCATTACACATCATAATAAATTAGGAATAGATATGTATCTACGAATC
GCTCCAGAATTATATCTAAAAAAATTAGTGATAGGTGGTTTTGAGCGAATATTCGAAATAAATCGTAGTTTTCGTAATGA
AGGTATATCTTCTTATCATAACCCTGAGTTTACAATGATGGAAATATATATGGCTTATGCTGATTATCGCGATATAATCA
TCTTAGTGCAAAATTTATTAAGATCGGTAACACAGAAAATACTAGGTAGTAATATAATAAATTATGGAGATTATGAATTA
GATTTTAAGCATCCTTTTACGCAAATCAGCATAAAAGAAGCTATATTGTATTATCTACCAGAAACTAGATCCCAAAATAT
AGACGATATATGTACTGCTGTGTCTATCGCAAAATCACTTGGCATTAAAGTTAAGAGTTGTTGGACGTTACACAGAATAC
ATATGGTTATTTTTGAAGAAGTAATAGAAAAAAAAATAATTCAACCGACTTGTGTTACTTCTTATCCAATAGAAATATCT
CCATTAGCACGTCGTAATGATAACAATCCAGAGTTTGCTGATCGTTTTGAACTTTTCATTGCCGGAAGAGAAATAGGAAA
TGGCTTTTCAGAATTAAACGATCCAGAAGATCAAAAGGAACGTTTTTTAAAACAAGCATATGGGAAAAAAAATAAAATAA
ATAATAATAATGTTCACATACATTATGACGAAGATTATTTAATTGCATTAGAATATGGATTACCTCCTACAGCAGGTATT
GGGATAGGAATTGATCGTTTGATTATGTTGCTCACCGACAGCCATACTATTCGTGATGTAATTTTATTTCCTACACTTCG
TCCAAAATAA

Upstream 100 bases:

>100_bases
CGTACTGGTTTTGAAAAACGTAATGTTAAAGCTGTTTTAAATGGAGATCTAGATGATTTTATAAGAATTAGCATTAAAAA
CACTTTAAGTGAGATTCATA

Downstream 100 bases:

>100_bases
AATTTGTTTATGTTTTTTTAGTGTACTTAAAAGTAGTTATATATTTATGATTTTATCTGATTAATTTTTTTAATAATACA
AACCTATTTTTTACGATTTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTKYVYSKDQSYQKFNVDDELNIRRKKLSKLREKGVAFPNNFRRNSISNQLHKKYAHTSNVELIQLNIEVTIAGRIISQR
IMGKASFITIRDAEGCIQLYITSNSLATNLYDENIKQWDLGDILGARGILFRTRTGELSIYCKEIRLLTKSLRPLPDKFH
GLNNQETKYRQRYLDLIINENTRKTFKIRSLVISEIRQFMKKNNFMEVETPMMHTIAGGAIAHPFITHHNKLGIDMYLRI
APELYLKKLVIGGFERIFEINRSFRNEGISSYHNPEFTMMEIYMAYADYRDIIILVQNLLRSVTQKILGSNIINYGDYEL
DFKHPFTQISIKEAILYYLPETRSQNIDDICTAVSIAKSLGIKVKSCWTLHRIHMVIFEEVIEKKIIQPTCVTSYPIEIS
PLARRNDNNPEFADRFELFIAGREIGNGFSELNDPEDQKERFLKQAYGKKNKINNNNVHIHYDEDYLIALEYGLPPTAGI
GIGIDRLIMLLTDSHTIRDVILFPTLRPK

Sequences:

>Translated_509_residues
MTKYVYSKDQSYQKFNVDDELNIRRKKLSKLREKGVAFPNNFRRNSISNQLHKKYAHTSNVELIQLNIEVTIAGRIISQR
IMGKASFITIRDAEGCIQLYITSNSLATNLYDENIKQWDLGDILGARGILFRTRTGELSIYCKEIRLLTKSLRPLPDKFH
GLNNQETKYRQRYLDLIINENTRKTFKIRSLVISEIRQFMKKNNFMEVETPMMHTIAGGAIAHPFITHHNKLGIDMYLRI
APELYLKKLVIGGFERIFEINRSFRNEGISSYHNPEFTMMEIYMAYADYRDIIILVQNLLRSVTQKILGSNIINYGDYEL
DFKHPFTQISIKEAILYYLPETRSQNIDDICTAVSIAKSLGIKVKSCWTLHRIHMVIFEEVIEKKIIQPTCVTSYPIEIS
PLARRNDNNPEFADRFELFIAGREIGNGFSELNDPEDQKERFLKQAYGKKNKINNNNVHIHYDEDYLIALEYGLPPTAGI
GIGIDRLIMLLTDSHTIRDVILFPTLRPK
>Mature_508_residues
TKYVYSKDQSYQKFNVDDELNIRRKKLSKLREKGVAFPNNFRRNSISNQLHKKYAHTSNVELIQLNIEVTIAGRIISQRI
MGKASFITIRDAEGCIQLYITSNSLATNLYDENIKQWDLGDILGARGILFRTRTGELSIYCKEIRLLTKSLRPLPDKFHG
LNNQETKYRQRYLDLIINENTRKTFKIRSLVISEIRQFMKKNNFMEVETPMMHTIAGGAIAHPFITHHNKLGIDMYLRIA
PELYLKKLVIGGFERIFEINRSFRNEGISSYHNPEFTMMEIYMAYADYRDIIILVQNLLRSVTQKILGSNIINYGDYELD
FKHPFTQISIKEAILYYLPETRSQNIDDICTAVSIAKSLGIKVKSCWTLHRIHMVIFEEVIEKKIIQPTCVTSYPIEISP
LARRNDNNPEFADRFELFIAGREIGNGFSELNDPEDQKERFLKQAYGKKNKINNNNVHIHYDEDYLIALEYGLPPTAGIG
IGIDRLIMLLTDSHTIRDVILFPTLRPK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=510, Percent_Identity=40.1960784313725, Blast_Score=352, Evalue=7e-97,
Organism=Homo sapiens, GI194272210, Length=510, Percent_Identity=40.1960784313725, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI45439306, Length=462, Percent_Identity=20.7792207792208, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI40789249, Length=248, Percent_Identity=25, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789256, Length=493, Percent_Identity=62.2718052738337, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=60.9657947686117, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=28.7878787878788, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788173, Length=273, Percent_Identity=25.6410256410256, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535927, Length=509, Percent_Identity=39.2927308447937, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17535925, Length=509, Percent_Identity=39.2927308447937, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=459, Percent_Identity=41.6122004357298, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI32566633, Length=298, Percent_Identity=24.8322147651007, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=349, Percent_Identity=24.0687679083095, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=39.7260273972603, Blast_Score=345, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6324256, Length=486, Percent_Identity=29.6296296296296, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24640849, Length=521, Percent_Identity=38.5796545105566, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24640851, Length=521, Percent_Identity=38.5796545105566, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24584738, Length=314, Percent_Identity=25.796178343949, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_BLOPB (Q493E1)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277774.1
- ProteinModelPortal:   Q493E1
- SMR:   Q493E1
- STRING:   Q493E1
- GeneID:   3562610
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_270
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- BioCyc:   CBLO291272:BPEN_270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 59162; Mature: 59031

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYVYSKDQSYQKFNVDDELNIRRKKLSKLREKGVAFPNNFRRNSISNQLHKKYAHTSN
CCCEEECCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
VELIQLNIEVTIAGRIISQRIMGKASFITIRDAEGCIQLYITSNSLATNLYDENIKQWDL
CEEEEEEEEEEEHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCH
GDILGARGILFRTRTGELSIYCKEIRLLTKSLRPLPDKFHGLNNQETKYRQRYLDLIINE
HHHHCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCC
NTRKTFKIRSLVISEIRQFMKKNNFMEVETPMMHTIAGGAIAHPFITHHNKLGIDMYLRI
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHCCHHHCCCCCCCEEEEEH
APELYLKKLVIGGFERIFEINRSFRNEGISSYHNPEFTMMEIYMAYADYRDIIILVQNLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSVTQKILGSNIINYGDYELDFKHPFTQISIKEAILYYLPETRSQNIDDICTAVSIAKSL
HHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GIKVKSCWTLHRIHMVIFEEVIEKKIIQPTCVTSYPIEISPLARRNDNNPEFADRFELFI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHCCCCCCCCHHHHEEEEE
AGREIGNGFSELNDPEDQKERFLKQAYGKKNKINNNNVHIHYDEDYLIALEYGLPPTAGI
ECHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCC
GIGIDRLIMLLTDSHTIRDVILFPTLRPK
CCCHHHHHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TKYVYSKDQSYQKFNVDDELNIRRKKLSKLREKGVAFPNNFRRNSISNQLHKKYAHTSN
CCEEECCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
VELIQLNIEVTIAGRIISQRIMGKASFITIRDAEGCIQLYITSNSLATNLYDENIKQWDL
CEEEEEEEEEEEHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCCCH
GDILGARGILFRTRTGELSIYCKEIRLLTKSLRPLPDKFHGLNNQETKYRQRYLDLIINE
HHHHCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCC
NTRKTFKIRSLVISEIRQFMKKNNFMEVETPMMHTIAGGAIAHPFITHHNKLGIDMYLRI
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCHHHCCHHHCCCCCCCEEEEEH
APELYLKKLVIGGFERIFEINRSFRNEGISSYHNPEFTMMEIYMAYADYRDIIILVQNLL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSVTQKILGSNIINYGDYELDFKHPFTQISIKEAILYYLPETRSQNIDDICTAVSIAKSL
HHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
GIKVKSCWTLHRIHMVIFEEVIEKKIIQPTCVTSYPIEISPLARRNDNNPEFADRFELFI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHCCCCCCCCHHHHEEEEE
AGREIGNGFSELNDPEDQKERFLKQAYGKKNKINNNNVHIHYDEDYLIALEYGLPPTAGI
ECHHHCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCC
GIGIDRLIMLLTDSHTIRDVILFPTLRPK
CCCHHHHHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA