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The map label for this gene is prfB [H]

Identifier: 71892043

GI number: 71892043

Start: 306663

End: 307709

Strand: Direct

Name: prfB [H]

Synonym: BPEN_269

Alternate gene names: 71892043

Gene position: 306663-307709 (Clockwise)

Preceding gene: 71892041

Following gene: 71892044

Centisome position: 38.74

GC content: 31.52

Gene sequence:

>1047_bases
ATGATTTTTGATTACGATCTCAAAATCAAACATTTTAAAATAATTAGCAACGAACTAAAATCACCCGGTATTTGGAAAAA
TCCTAAAACAGCAAAAATTCTTAGACAAAAATCATCTTCTTTAAAAATTTTTATCAATATTGTTGATCAGCTGTATAAAA
ATTTAGTGGATTTAAATGATTTGTTAGAATTGACGGAAGAATACGACAACATCTTACTTTCCGAAATTCATGATCAATCA
ATTGATTTAGAACGTACATTATCTCAATTAGAGATTAGTCGTATGTTTATAGGAAAGTATGATAATTCTAATTGTTATAT
CGATATTCAATCAGGATCCGGCGGAATAGAAGCACAGGATTGGGCCAGTATGTTGTTACGCATGTATTTACGTTGGATTG
ATCACAAAGGTTTTATGGCTGACGTTATTGAAGAATCGACAGGAGAGGTCACGGGGATAAAATCTGCTACTATTCAGGTT
ATTGGGCCTTATGCATATGGTTGGTTACGTACTGAATCTGGTGTACACAGACTAGTGCGCAAAAGTCCTTTTGACTCTGC
TAAGAAACGCCATACTTCATTTGCTTCAGTATTTGTATATCCAGAATTAGATGATAGTATAAATATTCATATTCGTCCAG
AAGATCTACGTTGTGATGTTTACCGAGCTTCTGGAGCAGGAGGTCAACATGTTAACCGTACAGAATCTGCAGTACGCATC
ACTCATATACCAACAAATATTGTTACCCAATGTCAAAGTGATCGTTCACAACATAAAAATAAAAATCAAGCTATGAAGCA
ACTAAGAGCTAAATTATATGAGCTTGAATTACAAAAAAAAAATTATGAAAAAAAAATGAGAGAAAGTAATAAAGTAAATA
TTACTTGGGGTAATCAAATACGATCTTATATTTTAGATAATTCTAGAGTTAAAGATTTACGTACTGGTTTTGAAAAACGT
AATGTTAAAGCTGTTTTAAATGGAGATCTAGATGATTTTATAAGAATTAGCATTAAAAACACTTTAAGTGAGATTCATAA
TGACTAA

Upstream 100 bases:

>100_bases
AATAAAAATATATATCTTAAGGAATAAAATTTTATGATAGATATCGATTTTACTAAACAACATATTCAAGTTATGTTAAA
TAGAATATCGAAGTTTAAGG

Downstream 100 bases:

>100_bases
ATACGTATATTCAAAGGATCAGTCATATCAAAAATTTAATGTCGATGATGAATTAAATATTCGTCGAAAAAAACTATCAA
AACTCCGTGAAAAAGGCGTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIFDYDLKIKHFKIISNELKSPGIWKNPKTAKILRQKSSSLKIFINIVDQLYKNLVDLNDLLELTEEYDNILLSEIHDQS
IDLERTLSQLEISRMFIGKYDNSNCYIDIQSGSGGIEAQDWASMLLRMYLRWIDHKGFMADVIEESTGEVTGIKSATIQV
IGPYAYGWLRTESGVHRLVRKSPFDSAKKRHTSFASVFVYPELDDSINIHIRPEDLRCDVYRASGAGGQHVNRTESAVRI
THIPTNIVTQCQSDRSQHKNKNQAMKQLRAKLYELELQKKNYEKKMRESNKVNITWGNQIRSYILDNSRVKDLRTGFEKR
NVKAVLNGDLDDFIRISIKNTLSEIHND

Sequences:

>Translated_348_residues
MIFDYDLKIKHFKIISNELKSPGIWKNPKTAKILRQKSSSLKIFINIVDQLYKNLVDLNDLLELTEEYDNILLSEIHDQS
IDLERTLSQLEISRMFIGKYDNSNCYIDIQSGSGGIEAQDWASMLLRMYLRWIDHKGFMADVIEESTGEVTGIKSATIQV
IGPYAYGWLRTESGVHRLVRKSPFDSAKKRHTSFASVFVYPELDDSINIHIRPEDLRCDVYRASGAGGQHVNRTESAVRI
THIPTNIVTQCQSDRSQHKNKNQAMKQLRAKLYELELQKKNYEKKMRESNKVNITWGNQIRSYILDNSRVKDLRTGFEKR
NVKAVLNGDLDDFIRISIKNTLSEIHND
>Mature_348_residues
MIFDYDLKIKHFKIISNELKSPGIWKNPKTAKILRQKSSSLKIFINIVDQLYKNLVDLNDLLELTEEYDNILLSEIHDQS
IDLERTLSQLEISRMFIGKYDNSNCYIDIQSGSGGIEAQDWASMLLRMYLRWIDHKGFMADVIEESTGEVTGIKSATIQV
IGPYAYGWLRTESGVHRLVRKSPFDSAKKRHTSFASVFVYPELDDSINIHIRPEDLRCDVYRASGAGGQHVNRTESAVRI
THIPTNIVTQCQSDRSQHKNKNQAMKQLRAKLYELELQKKNYEKKMRESNKVNITWGNQIRSYILDNSRVKDLRTGFEKR
NVKAVLNGDLDDFIRISIKNTLSEIHND

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=278, Percent_Identity=34.5323741007194, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI34577120, Length=245, Percent_Identity=35.1020408163265, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=35.4838709677419, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=61.6279069767442, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=38.2716049382716, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=264, Percent_Identity=33.7121212121212, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=293, Percent_Identity=35.4948805460751, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19921226, Length=222, Percent_Identity=37.3873873873874, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40243; Mature: 40243

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDYDLKIKHFKIISNELKSPGIWKNPKTAKILRQKSSSLKIFINIVDQLYKNLVDLND
CEEEECCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHCHHH
LLELTEEYDNILLSEIHDQSIDLERTLSQLEISRMFIGKYDNSNCYIDIQSGSGGIEAQD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCHHH
WASMLLRMYLRWIDHKGFMADVIEESTGEVTGIKSATIQVIGPYAYGWLRTESGVHRLVR
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCEEEEECCHHHEEEECHHHHHHHHH
KSPFDSAKKRHTSFASVFVYPELDDSINIHIRPEDLRCDVYRASGAGGQHVNRTESAVRI
CCCCHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEE
THIPTNIVTQCQSDRSQHKNKNQAMKQLRAKLYELELQKKNYEKKMRESNKVNITWGNQI
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
RSYILDNSRVKDLRTGFEKRNVKAVLNGDLDDFIRISIKNTLSEIHND
HHHHHCCCHHHHHHCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFDYDLKIKHFKIISNELKSPGIWKNPKTAKILRQKSSSLKIFINIVDQLYKNLVDLND
CEEEECCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHCHHH
LLELTEEYDNILLSEIHDQSIDLERTLSQLEISRMFIGKYDNSNCYIDIQSGSGGIEAQD
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCCHHH
WASMLLRMYLRWIDHKGFMADVIEESTGEVTGIKSATIQVIGPYAYGWLRTESGVHRLVR
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCCEEEEECCHHHEEEECHHHHHHHHH
KSPFDSAKKRHTSFASVFVYPELDDSINIHIRPEDLRCDVYRASGAGGQHVNRTESAVRI
CCCCHHHHHHCCCEEEEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCEEEE
THIPTNIVTQCQSDRSQHKNKNQAMKQLRAKLYELELQKKNYEKKMRESNKVNITWGNQI
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
RSYILDNSRVKDLRTGFEKRNVKAVLNGDLDDFIRISIKNTLSEIHND
HHHHHCCCHHHHHHCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA