Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 71891962

GI number: 71891962

Start: 227992

End: 228759

Strand: Direct

Name: trmD

Synonym: BPEN_181

Alternate gene names: 71891962

Gene position: 227992-228759 (Clockwise)

Preceding gene: 71891961

Following gene: 71891963

Centisome position: 28.8

GC content: 33.98

Gene sequence:

>768_bases
ATGTTGTTAGGTGTGATTACTTTATTCCCAGATATGTTTCAATCTATTGTTCGTTATGGAATAGTAGGTCGAGCAATTCG
AAGGGGTATTCTTTCCATAAAATTGTGGAATCCACGTCTGTTTACATATGATCGACACCACAGCGTAGATGCCCGTCCCT
ATGGAGGAGGACCAGGCATGTTAATGATGATTGAACCCCTGAGAAATGCAATCAATCAAGCTAAAGATGAATTAGGAAAT
AATATTAAAGTGATTTATCTTTCTCCTCAAGGAAGAAAGCTAAAGCAGAAATATGTATATAAGTTAGCATATGATCATCA
GAAATTAATTTTAGTTTGTGGTAGATATCAAGGCATTGATGAAAGATTAATTCAAACGGAAATTGACGAAGAGTGGTCTA
TAGGAGATTATATACTTAGCGGAGGAGAGTTAGCAGCAATGGTATTAATAGATACGATATCTAGAGTTCTGCCTGGCGTA
TTAGGAAATCAAGATTCAAAAGAATCAGATTCCTTTTCCAAAGAAAGATTAGATTGCCCACATTATACACGACCAGAAAC
TTTTGACGGCATGAAAGTACCGTCGGTATTATTATCAGGAAATCATGATGAAATTCATCGTTGGAAACAAAAACAAGCTT
TGGGTCGTACTTGGATTAAAAGGCCGGATTTGCTTAATTATATACAATTAACTAATGAGGAAAAAAATTTACTTTCTGAA
TTTAAAAATGAATACTTGCTATCATTAAATAAAAAAACTAGGAAATAA

Upstream 100 bases:

>100_bases
TTATCAAGAGAGTTATTAAAAATATCAATCTTGTCACACGTACTGTTACCGTAGATTGGGATCCTAATTTTTAGTTTTTT
AAAATAAAGTATAAAAAAAT

Downstream 100 bases:

>100_bases
TATATATATGTACGAATTAATAAATAATTTCGAAAAAAAACAAATGAAAAAAAATATACCGCACTTACATCCAGGTGATA
CAGTGGAAGTGAAAACATGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLLGVITLFPDMFQSIVRYGIVGRAIRRGILSIKLWNPRLFTYDRHHSVDARPYGGGPGMLMMIEPLRNAINQAKDELGN
NIKVIYLSPQGRKLKQKYVYKLAYDHQKLILVCGRYQGIDERLIQTEIDEEWSIGDYILSGGELAAMVLIDTISRVLPGV
LGNQDSKESDSFSKERLDCPHYTRPETFDGMKVPSVLLSGNHDEIHRWKQKQALGRTWIKRPDLLNYIQLTNEEKNLLSE
FKNEYLLSLNKKTRK

Sequences:

>Translated_255_residues
MLLGVITLFPDMFQSIVRYGIVGRAIRRGILSIKLWNPRLFTYDRHHSVDARPYGGGPGMLMMIEPLRNAINQAKDELGN
NIKVIYLSPQGRKLKQKYVYKLAYDHQKLILVCGRYQGIDERLIQTEIDEEWSIGDYILSGGELAAMVLIDTISRVLPGV
LGNQDSKESDSFSKERLDCPHYTRPETFDGMKVPSVLLSGNHDEIHRWKQKQALGRTWIKRPDLLNYIQLTNEEKNLLSE
FKNEYLLSLNKKTRK
>Mature_255_residues
MLLGVITLFPDMFQSIVRYGIVGRAIRRGILSIKLWNPRLFTYDRHHSVDARPYGGGPGMLMMIEPLRNAINQAKDELGN
NIKVIYLSPQGRKLKQKYVYKLAYDHQKLILVCGRYQGIDERLIQTEIDEEWSIGDYILSGGELAAMVLIDTISRVLPGV
LGNQDSKESDSFSKERLDCPHYTRPETFDGMKVPSVLLSGNHDEIHRWKQKQALGRTWIKRPDLLNYIQLTNEEKNLLSE
FKNEYLLSLNKKTRK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=245, Percent_Identity=65.3061224489796, Blast_Score=333, Evalue=5e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BLOPB (Q493M1)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277692.1
- ProteinModelPortal:   Q493M1
- SMR:   Q493M1
- STRING:   Q493M1
- GeneID:   3562676
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_181
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   CBLO291272:BPEN_181-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29422; Mature: 29422

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGVITLFPDMFQSIVRYGIVGRAIRRGILSIKLWNPRLFTYDRHHSVDARPYGGGPGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCC
LMMIEPLRNAINQAKDELGNNIKVIYLSPQGRKLKQKYVYKLAYDHQKLILVCGRYQGID
EEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH
ERLIQTEIDEEWSIGDYILSGGELAAMVLIDTISRVLPGVLGNQDSKESDSFSKERLDCP
HHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC
HYTRPETFDGMKVPSVLLSGNHDEIHRWKQKQALGRTWIKRPDLLNYIQLTNEEKNLLSE
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHH
FKNEYLLSLNKKTRK
HHHHHEEECCHHCCC
>Mature Secondary Structure
MLLGVITLFPDMFQSIVRYGIVGRAIRRGILSIKLWNPRLFTYDRHHSVDARPYGGGPGM
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEEEECCCCCCCCCCCCCCCCC
LMMIEPLRNAINQAKDELGNNIKVIYLSPQGRKLKQKYVYKLAYDHQKLILVCGRYQGID
EEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCHH
ERLIQTEIDEEWSIGDYILSGGELAAMVLIDTISRVLPGVLGNQDSKESDSFSKERLDCP
HHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC
HYTRPETFDGMKVPSVLLSGNHDEIHRWKQKQALGRTWIKRPDLLNYIQLTNEEKNLLSE
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHH
FKNEYLLSLNKKTRK
HHHHHEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA