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The map label for this gene is pnp

Identifier: 71891895

GI number: 71891895

Start: 130316

End: 132415

Strand: Direct

Name: pnp

Synonym: BPEN_112

Alternate gene names: 71891895

Gene position: 130316-132415 (Clockwise)

Preceding gene: 71891894

Following gene: 71891896

Centisome position: 16.46

GC content: 33.81

Gene sequence:

>2100_bases
TTGTTAGATCCGATTATTCATAAATTTAAATATGGACAGCATACTGTAATTTTAGATATTGGAACCATAGCTCGTCAAGC
AAATGCTGCAGTCATGGTGAGTATGAGTGATACTACAGTATTAGTTACAGTAGTTAGTTCTAATCAAGTCAGATTAGAAC
AAGATTTTTTTCCTCTTGTTGTAAATTATCAAGAACGCACTTATGCTGCTGGAAAATTTCCAGGAGGTTTTTTTCGTCGA
GAAGGTCGACCAAGTGAAAACGAAACATTAACGTCTCGCTTGATTGATCGCCCTATTCGGCCGTTATTCCCTAAGGGATT
TATTAATGATGTACAAGTAGTTGCTACGGTAGTATCAGTAAATCCGCAAGTAAATCCAGATATTGTAGCAATAATAGGAG
TATCTGCTGCATTGAGTTTGTCTGATATTCCATTTTTTGGACCCATTGGAGCAGCTAGAGTTGGTTATATTAAAAATCAA
TATGTATTAAATCCAACAACAACAGAGTTATCTAATAGTAAGTTAGATTTGGTGATTTCAGGGACTGAATCTAATATCTT
GATGGTGGAATCAGAATCGTGTTTACTAAGTGAAAAAGAAATTTTAGACGCTATTATATTTGGTCACGAACAACAACAAA
TAGTGATTCATAATATTAATAAATTAGTTAAAAAAGTTGGAAAAACTAAGTATTCTTGGAACGAAAAGGAAGTGAACATA
TCTTTAAAAACGTATTTATCTGAATTGTATGAGTCTCGTGCGCAAGATATATATTGTTTTTTTGATAAAAAAGAGCGTCA
TGCTCAAGTTGATAGTATTAAGAAAGATATAGTAAAAACCGTTCTTAATAGCTACCAGCATGCTGCTGTAGATGAAAAAG
AAATAATGTATCTTTTAAATTATTTAGAAGCGCAATCAATTCGTTATCGTATTTTAACGAACAAATTACGTATTGATGGA
CGTTCACAAGATACAATTCGGAAAATAGATATAAAAACAGGCATATTACCACGTACTCATGGATCAGCATTATTTACTAG
AGGAGATACTCAAGCGCTGGTTGCTGTTACGCTTGGCACAGAACGTGATGCACAGAATATTGATGGATTAACTGGTATTC
GTGTGGATAGATTTTTATTACATTATAATTTTCCACCATATTGTGTAGGTGAAATAGGTGTCATAGGAGCTCCAAAAAGG
AGAGAAATAGGACATGGACGTTTAGCCAAACGGGGTATGTTAGCAGTTATGCCTAATGCTAATGAGTTTCCGTATACAAT
ACGTGTAGTATCAGAAATTACTGAATCTAATGGATCGTCCTCCATGGCATCTATTTGTGGTACATCATTAGCGTTGATGG
ATGCTGGAGTACCGATTAAAGCAGCAGTTGCTGGTGTTGCTATGGGTCTGATTAAGGAAGGAAACGATTTCGTGGTATTA
TCTGATATTGTTAGCGATGAAGATCATATTGGAGATATGGATTTTAAAGTATCTGGTAGTCGTCAAGGCATAACAGCATT
GCAAATGGATATTAAAACGGACGGGATTACGTATGATATAATAAATATTGCGTTAAAAAAAGCAAAAAATGCTCGACTGC
AAATATTAGATGTTATGGAACAAATTATTAAGTTTCCTAATCAGGAAATCTCTAAATTTGCTCCCAGGATATATAGCATT
AAAGTTAATCCAGATAAAATTAAAGATGTTATAGGAAAGGGAGGGTCAGTTATTCGCTCATTGACTGAAGAAACTAATAC
TATTATTGATATTGAAGATAATGGTATCATAAAAATAGTTGCTTTAGATTATGATAAAGCAAAACAAGCTATTCGTAGAA
TTAATGATATCACTGCAAACGTTGAAATAGGAGCCGTTTATACTGGAAAAGTTAGTCACATTGTGGAATTTGGGGCATTC
GTTACTATCCTTTCTGGAAAAGAAGGGTTAGTTCATATTTCCCAGATTTCTGAAAGAAGAATAAATAAAGTAACTGATTA
TTTGAAATTAGGGCAAAAAGTATTAGTAAAAGTGTTAGAAATAGATCGTCAGGGACGAATTCGTTTGAGTATGAAAGGAG
TACATCAAGTGAGTGCATAG

Upstream 100 bases:

>100_bases
AAATGAAATTTTTTTAATTTTATATTTAAGATCATGATGTTCTTATGATTGTTTAGACATATTAAAAAATTATATATTAA
ATTATAAAAAGGATATTATT

Downstream 100 bases:

>100_bases
CACATAGTATTTTACTGTGATAAAATTTTATATGTTTGCGTATGTGAGATAATTGTAGTTGCACGGAGGATTACATAAAA
AAGATTGGTGACAATTCAAG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MLDPIIHKFKYGQHTVILDIGTIARQANAAVMVSMSDTTVLVTVVSSNQVRLEQDFFPLVVNYQERTYAAGKFPGGFFRR
EGRPSENETLTSRLIDRPIRPLFPKGFINDVQVVATVVSVNPQVNPDIVAIIGVSAALSLSDIPFFGPIGAARVGYIKNQ
YVLNPTTTELSNSKLDLVISGTESNILMVESESCLLSEKEILDAIIFGHEQQQIVIHNINKLVKKVGKTKYSWNEKEVNI
SLKTYLSELYESRAQDIYCFFDKKERHAQVDSIKKDIVKTVLNSYQHAAVDEKEIMYLLNYLEAQSIRYRILTNKLRIDG
RSQDTIRKIDIKTGILPRTHGSALFTRGDTQALVAVTLGTERDAQNIDGLTGIRVDRFLLHYNFPPYCVGEIGVIGAPKR
REIGHGRLAKRGMLAVMPNANEFPYTIRVVSEITESNGSSSMASICGTSLALMDAGVPIKAAVAGVAMGLIKEGNDFVVL
SDIVSDEDHIGDMDFKVSGSRQGITALQMDIKTDGITYDIINIALKKAKNARLQILDVMEQIIKFPNQEISKFAPRIYSI
KVNPDKIKDVIGKGGSVIRSLTEETNTIIDIEDNGIIKIVALDYDKAKQAIRRINDITANVEIGAVYTGKVSHIVEFGAF
VTILSGKEGLVHISQISERRINKVTDYLKLGQKVLVKVLEIDRQGRIRLSMKGVHQVSA

Sequences:

>Translated_699_residues
MLDPIIHKFKYGQHTVILDIGTIARQANAAVMVSMSDTTVLVTVVSSNQVRLEQDFFPLVVNYQERTYAAGKFPGGFFRR
EGRPSENETLTSRLIDRPIRPLFPKGFINDVQVVATVVSVNPQVNPDIVAIIGVSAALSLSDIPFFGPIGAARVGYIKNQ
YVLNPTTTELSNSKLDLVISGTESNILMVESESCLLSEKEILDAIIFGHEQQQIVIHNINKLVKKVGKTKYSWNEKEVNI
SLKTYLSELYESRAQDIYCFFDKKERHAQVDSIKKDIVKTVLNSYQHAAVDEKEIMYLLNYLEAQSIRYRILTNKLRIDG
RSQDTIRKIDIKTGILPRTHGSALFTRGDTQALVAVTLGTERDAQNIDGLTGIRVDRFLLHYNFPPYCVGEIGVIGAPKR
REIGHGRLAKRGMLAVMPNANEFPYTIRVVSEITESNGSSSMASICGTSLALMDAGVPIKAAVAGVAMGLIKEGNDFVVL
SDIVSDEDHIGDMDFKVSGSRQGITALQMDIKTDGITYDIINIALKKAKNARLQILDVMEQIIKFPNQEISKFAPRIYSI
KVNPDKIKDVIGKGGSVIRSLTEETNTIIDIEDNGIIKIVALDYDKAKQAIRRINDITANVEIGAVYTGKVSHIVEFGAF
VTILSGKEGLVHISQISERRINKVTDYLKLGQKVLVKVLEIDRQGRIRLSMKGVHQVSA
>Mature_699_residues
MLDPIIHKFKYGQHTVILDIGTIARQANAAVMVSMSDTTVLVTVVSSNQVRLEQDFFPLVVNYQERTYAAGKFPGGFFRR
EGRPSENETLTSRLIDRPIRPLFPKGFINDVQVVATVVSVNPQVNPDIVAIIGVSAALSLSDIPFFGPIGAARVGYIKNQ
YVLNPTTTELSNSKLDLVISGTESNILMVESESCLLSEKEILDAIIFGHEQQQIVIHNINKLVKKVGKTKYSWNEKEVNI
SLKTYLSELYESRAQDIYCFFDKKERHAQVDSIKKDIVKTVLNSYQHAAVDEKEIMYLLNYLEAQSIRYRILTNKLRIDG
RSQDTIRKIDIKTGILPRTHGSALFTRGDTQALVAVTLGTERDAQNIDGLTGIRVDRFLLHYNFPPYCVGEIGVIGAPKR
REIGHGRLAKRGMLAVMPNANEFPYTIRVVSEITESNGSSSMASICGTSLALMDAGVPIKAAVAGVAMGLIKEGNDFVVL
SDIVSDEDHIGDMDFKVSGSRQGITALQMDIKTDGITYDIINIALKKAKNARLQILDVMEQIIKFPNQEISKFAPRIYSI
KVNPDKIKDVIGKGGSVIRSLTEETNTIIDIEDNGIIKIVALDYDKAKQAIRRINDITANVEIGAVYTGKVSHIVEFGAF
VTILSGKEGLVHISQISERRINKVTDYLKLGQKVLVKVLEIDRQGRIRLSMKGVHQVSA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=705, Percent_Identity=38.4397163120567, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=68.2080924855491, Blast_Score=997, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=707, Percent_Identity=31.1173974540311, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17535281, Length=82, Percent_Identity=50, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=106, Percent_Identity=35.8490566037736, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=37.7331420373027, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=37.7331420373027, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=37.7331420373027, Blast_Score=458, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=656, Percent_Identity=37.3475609756098, Blast_Score=425, Evalue=1e-119,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BLOPB (Q493T3)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277625.1
- HSSP:   P05198
- ProteinModelPortal:   Q493T3
- SMR:   Q493T3
- STRING:   Q493T3
- GeneID:   3563170
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_112
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   CBLO291272:BPEN_112-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77478; Mature: 77478

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPIIHKFKYGQHTVILDIGTIARQANAAVMVSMSDTTVLVTVVSSNQVRLEQDFFPLV
CCCHHHHHHCCCCEEEEEEEHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEECCCEEEE
VNYQERTYAAGKFPGGFFRREGRPSENETLTSRLIDRPIRPLFPKGFINDVQVVATVVSV
EEECHHEEECCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEEC
NPQVNPDIVAIIGVSAALSLSDIPFFGPIGAARVGYIKNQYVLNPTTTELSNSKLDLVIS
CCCCCCCEEEEEECHHEEEECCCCCCCCCCCHHHCEECCCEEECCCCCCCCCCEEEEEEE
GTESNILMVESESCLLSEKEILDAIIFGHEQQQIVIHNINKLVKKVGKTKYSWNEKEVNI
CCCCCEEEEECCCHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEE
SLKTYLSELYESRAQDIYCFFDKKERHAQVDSIKKDIVKTVLNSYQHAAVDEKEIMYLLN
EHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YLEAQSIRYRILTNKLRIDGRSQDTIRKIDIKTGILPRTHGSALFTRGDTQALVAVTLGT
HHHHHCEEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEEEECC
ERDAQNIDGLTGIRVDRFLLHYNFPPYCVGEIGVIGAPKRREIGHGRLAKRGMLAVMPNA
CCCCCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHCCEEEECCCC
NEFPYTIRVVSEITESNGSSSMASICGTSLALMDAGVPIKAAVAGVAMGLIKEGNDFVVL
CCCCEEEEEEHHHHHCCCCCHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEE
SDIVSDEDHIGDMDFKVSGSRQGITALQMDIKTDGITYDIINIALKKAKNARLQILDVME
EECCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCHHHHHHHHH
QIIKFPNQEISKFAPRIYSIKVNPDKIKDVIGKGGSVIRSLTEETNTIIDIEDNGIIKIV
HHHHCCCHHHHHHCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCEEEEE
ALDYDKAKQAIRRINDITANVEIGAVYTGKVSHIVEFGAFVTILSGKEGLVHISQISERR
EECCHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHCCEEEEEECCCCEEEHHHHHHHH
INKVTDYLKLGQKVLVKVLEIDRQGRIRLSMKGVHQVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCC
>Mature Secondary Structure
MLDPIIHKFKYGQHTVILDIGTIARQANAAVMVSMSDTTVLVTVVSSNQVRLEQDFFPLV
CCCHHHHHHCCCCEEEEEEEHHHHCCCCCEEEEEECCCEEEEEEECCCCEEEECCCEEEE
VNYQERTYAAGKFPGGFFRREGRPSENETLTSRLIDRPIRPLFPKGFINDVQVVATVVSV
EEECHHEEECCCCCCCHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHEEEEC
NPQVNPDIVAIIGVSAALSLSDIPFFGPIGAARVGYIKNQYVLNPTTTELSNSKLDLVIS
CCCCCCCEEEEEECHHEEEECCCCCCCCCCCHHHCEECCCEEECCCCCCCCCCEEEEEEE
GTESNILMVESESCLLSEKEILDAIIFGHEQQQIVIHNINKLVKKVGKTKYSWNEKEVNI
CCCCCEEEEECCCHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEEE
SLKTYLSELYESRAQDIYCFFDKKERHAQVDSIKKDIVKTVLNSYQHAAVDEKEIMYLLN
EHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YLEAQSIRYRILTNKLRIDGRSQDTIRKIDIKTGILPRTHGSALFTRGDTQALVAVTLGT
HHHHHCEEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCEEEEEEECC
ERDAQNIDGLTGIRVDRFLLHYNFPPYCVGEIGVIGAPKRREIGHGRLAKRGMLAVMPNA
CCCCCCCCCCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHCCEEEECCCC
NEFPYTIRVVSEITESNGSSSMASICGTSLALMDAGVPIKAAVAGVAMGLIKEGNDFVVL
CCCCEEEEEEHHHHHCCCCCHHHHHHCCHHEEHCCCCCHHHHHHHHHHHHHCCCCCEEEE
SDIVSDEDHIGDMDFKVSGSRQGITALQMDIKTDGITYDIINIALKKAKNARLQILDVME
EECCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCCCHHHHHHHHH
QIIKFPNQEISKFAPRIYSIKVNPDKIKDVIGKGGSVIRSLTEETNTIIDIEDNGIIKIV
HHHHCCCHHHHHHCCEEEEEEECHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCEEEEE
ALDYDKAKQAIRRINDITANVEIGAVYTGKVSHIVEFGAFVTILSGKEGLVHISQISERR
EECCHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHCCEEEEEECCCCEEEHHHHHHHH
INKVTDYLKLGQKVLVKVLEIDRQGRIRLSMKGVHQVSA
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA