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The map label for this gene is nusA [H]

Identifier: 71891890

GI number: 71891890

Start: 124047

End: 125564

Strand: Direct

Name: nusA [H]

Synonym: BPEN_107

Alternate gene names: 71891890

Gene position: 124047-125564 (Clockwise)

Preceding gene: 71891889

Following gene: 71891891

Centisome position: 15.67

GC content: 33.27

Gene sequence:

>1518_bases
ATGAATAAAGAGATTTTGGCTGTTATAGAAGCAGTTTCTAATGAAAAAGCTGTACCTCAAGAAAAAATTTTTGAAGCATT
AGAAACAGCATTGGCTGCCGCTACAAAAAAGAAATATGAACAAGATGTTGAAGTTCGTGTTGTAATTGATCGTAAATCAG
GTCAGATTAATACATTTCGGAGGTGGGTTGTAGTGGAACAAGTAACTCAACCTACTAAAGAAATTACATTAGATGCAGCT
CGATTTGAGAATCCAAAAGTACAAATTTGTGATTACGTTGAAGATGTCATTGAATCTATTAGTTTCGATCGTATTACAAC
TCAAACCGCAAAACAAGTTATTGTTCAAAAGGTGCGTGAAGCTGAACGTGCAATTATTATAGAACAGTTTTTAAATCGAC
AGGGTGATATTGTTACAGGTATAGTAAAAAAAATTAATAGAGATAGTATAAGTATAGATTTAGGAAATAATGCTGAAGGT
ATGATTAATAGAGAAGAAATGTTACCGCGTGAAAATTTTAGATTAGGAGATAGAATTCGTGGTATTCTTTATTTGGTGCG
ACCGGATTTTAAAGGTCCTCAATTACTTATCAGTCGTACTAGGACTGAAATGTTGATTGAATTATTTCGTATTGAGGTAC
CTGAAATTGGAGAAGAATTAATCACAATTAAAGCCGCAGCGCGAGATCCTGGTTCACGGGCAAAAATTGCCGTCAAAACA
AATGATAAGCGTATTGATCCAGTAGGAGCATGTGTAGGAATGAGAGGAGCACGTGTTCAGGCTGTGTCTGGTGAATTAGG
AGGAGAACGAGTAGATGTTATATTATGGGATGATAATCCTGTTCAATTTGTTATCAATGCTATGTCTCCTGCTGATGTTG
CGTCTATTGTGATAGATGAAGATAAACATACAATGGATATTTCAGTAGAGGAAACTAATTTACCTCAGGCTATTGGAAGG
AATGGACAAAACATTCGTTTAGTATCTCAATTAAGCGGCTGGGAACTCAATATTATGACTGTAGATGAGTTGGAAAAGAA
ACGTCAAGTTGAAATATGTACTATCACAAACATTTTTATTCATTCCTTAAATATCAGTGAGCAATTTGCTAAAATTTTAA
TTGATTCAGGGTTTTCTTCTCTTGAGGAGTTAGCTTATGTACCTATGACAGAATTATTATCAATAAAAGAATTTGATAGA
AAAACAGTAGAAATAATTAGGGATCGCGCAAAAAGTGCTTTAAAGAATTTTTCTGTGATTCATAATGAAAAAAATATTAA
GGAAGATAATTATACTGCGGCGGTTATTCATCCCGTTACAGGATTATTGAGATTGCCTAATATTGAATATAAATTAGCAC
TAACATTAGTTGAACGTGGTATTTCTACATTAGAAGAGTTAGCTGAACAAGATATTTCGGATCTTTCCGATATTGAAGGA
TTAACTAAAGAAAAAATTGGAGAATTAATTATGGAAGCCCGCAATATTTGTTGGTTTAATAATGATAACAAACATTAA

Upstream 100 bases:

>100_bases
GTTTTCAATACGATGTAGGGGTGAATTGTTTTTTAAAATTTAATTACACAATGATATACACATAATAGCTATTAAGGAAA
TAGCATTTTGAGAAACTGGG

Downstream 100 bases:

>100_bases
TATTATTATTAGTAATAATTAAGGAAAGCCATGACAGATACCACCATACAATCGTTTGCTGCGGAAATGAAAATGTCAGT
AGATCAATTAATACAATGGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNKEILAVIEAVSNEKAVPQEKIFEALETALAAATKKKYEQDVEVRVVIDRKSGQINTFRRWVVVEQVTQPTKEITLDAA
RFENPKVQICDYVEDVIESISFDRITTQTAKQVIVQKVREAERAIIIEQFLNRQGDIVTGIVKKINRDSISIDLGNNAEG
MINREEMLPRENFRLGDRIRGILYLVRPDFKGPQLLISRTRTEMLIELFRIEVPEIGEELITIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSGELGGERVDVILWDDNPVQFVINAMSPADVASIVIDEDKHTMDISVEETNLPQAIGR
NGQNIRLVSQLSGWELNIMTVDELEKKRQVEICTITNIFIHSLNISEQFAKILIDSGFSSLEELAYVPMTELLSIKEFDR
KTVEIIRDRAKSALKNFSVIHNEKNIKEDNYTAAVIHPVTGLLRLPNIEYKLALTLVERGISTLEELAEQDISDLSDIEG
LTKEKIGELIMEARNICWFNNDNKH

Sequences:

>Translated_505_residues
MNKEILAVIEAVSNEKAVPQEKIFEALETALAAATKKKYEQDVEVRVVIDRKSGQINTFRRWVVVEQVTQPTKEITLDAA
RFENPKVQICDYVEDVIESISFDRITTQTAKQVIVQKVREAERAIIIEQFLNRQGDIVTGIVKKINRDSISIDLGNNAEG
MINREEMLPRENFRLGDRIRGILYLVRPDFKGPQLLISRTRTEMLIELFRIEVPEIGEELITIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSGELGGERVDVILWDDNPVQFVINAMSPADVASIVIDEDKHTMDISVEETNLPQAIGR
NGQNIRLVSQLSGWELNIMTVDELEKKRQVEICTITNIFIHSLNISEQFAKILIDSGFSSLEELAYVPMTELLSIKEFDR
KTVEIIRDRAKSALKNFSVIHNEKNIKEDNYTAAVIHPVTGLLRLPNIEYKLALTLVERGISTLEELAEQDISDLSDIEG
LTKEKIGELIMEARNICWFNNDNKH
>Mature_505_residues
MNKEILAVIEAVSNEKAVPQEKIFEALETALAAATKKKYEQDVEVRVVIDRKSGQINTFRRWVVVEQVTQPTKEITLDAA
RFENPKVQICDYVEDVIESISFDRITTQTAKQVIVQKVREAERAIIIEQFLNRQGDIVTGIVKKINRDSISIDLGNNAEG
MINREEMLPRENFRLGDRIRGILYLVRPDFKGPQLLISRTRTEMLIELFRIEVPEIGEELITIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSGELGGERVDVILWDDNPVQFVINAMSPADVASIVIDEDKHTMDISVEETNLPQAIGR
NGQNIRLVSQLSGWELNIMTVDELEKKRQVEICTITNIFIHSLNISEQFAKILIDSGFSSLEELAYVPMTELLSIKEFDR
KTVEIIRDRAKSALKNFSVIHNEKNIKEDNYTAAVIHPVTGLLRLPNIEYKLALTLVERGISTLEELAEQDISDLSDIEG
LTKEKIGELIMEARNICWFNNDNKH

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=502, Percent_Identity=68.7250996015936, Blast_Score=712, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 57069; Mature: 57069

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILAVIEAVSNEKAVPQEKIFEALETALAAATKKKYEQDVEVRVVIDRKSGQINTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
RWVVVEQVTQPTKEITLDAARFENPKVQICDYVEDVIESISFDRITTQTAKQVIVQKVRE
HHHHHHHHHCCHHHHEEEHHHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AERAIIIEQFLNRQGDIVTGIVKKINRDSISIDLGNNAEGMINREEMLPRENFRLGDRIR
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEHHHCCCCCCCCHHHHHC
GILYLVRPDFKGPQLLISRTRTEMLIELFRIEVPEIGEELITIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSGELGGERVDVILWDDNPVQFVINAMSPADVASIVIDE
CCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEC
DKHTMDISVEETNLPQAIGRNGQNIRLVSQLSGWELNIMTVDELEKKRQVEICTITNIFI
CCCEEEEEEECCCCCHHHCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCEEEEEEEEEEH
HSLNISEQFAKILIDSGFSSLEELAYVPMTELLSIKEFDRKTVEIIRDRAKSALKNFSVI
EECCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNEKNIKEDNYTAAVIHPVTGLLRLPNIEYKLALTLVERGISTLEELAEQDISDLSDIEG
CCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKEKIGELIMEARNICWFNNDNKH
HHHHHHHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure
MNKEILAVIEAVSNEKAVPQEKIFEALETALAAATKKKYEQDVEVRVVIDRKSGQINTFR
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHH
RWVVVEQVTQPTKEITLDAARFENPKVQICDYVEDVIESISFDRITTQTAKQVIVQKVRE
HHHHHHHHHCCHHHHEEEHHHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AERAIIIEQFLNRQGDIVTGIVKKINRDSISIDLGNNAEGMINREEMLPRENFRLGDRIR
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEHHHCCCCCCCCHHHHHC
GILYLVRPDFKGPQLLISRTRTEMLIELFRIEVPEIGEELITIKAAARDPGSRAKIAVKT
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSGELGGERVDVILWDDNPVQFVINAMSPADVASIVIDE
CCCCCCHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEC
DKHTMDISVEETNLPQAIGRNGQNIRLVSQLSGWELNIMTVDELEKKRQVEICTITNIFI
CCCEEEEEEECCCCCHHHCCCCCCEEEEEECCCCEEEEEEHHHHHHCCCEEEEEEEEEEH
HSLNISEQFAKILIDSGFSSLEELAYVPMTELLSIKEFDRKTVEIIRDRAKSALKNFSVI
EECCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HNEKNIKEDNYTAAVIHPVTGLLRLPNIEYKLALTLVERGISTLEELAEQDISDLSDIEG
CCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTKEKIGELIMEARNICWFNNDNKH
HHHHHHHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]