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The map label for this gene is ispB [H]

Identifier: 71891880

GI number: 71891880

Start: 113396

End: 114367

Strand: Reverse

Name: ispB [H]

Synonym: BPEN_095

Alternate gene names: 71891880

Gene position: 114367-113396 (Counterclockwise)

Preceding gene: 71891903

Following gene: 71891879

Centisome position: 14.45

GC content: 33.85

Gene sequence:

>972_bases
ATGAATATTGATCAAATCTCCGAACTAACTGAACAAGATATGGAAGATGTGAATTCAGAAATTCGCACGCGATTAGCATC
TGAAATCACTTTAATTAACAAACTTGTTCAATACATTGTAGATAGTGGAGGAAAACGAATTAGACCCATGATTACCTTGC
TGACCGCAAGGGCGTTATGCTATAAAAAAACACAACATGTTACTATTGCTACATTAATAGAGTTTATTCATACTGCTACT
TTATTACATGATGACGTAGTAGATAAATCACACATGAGGCGCGGTAGGAAAACTACTAATATAATTTTTGGTAATGCTGC
TAGTGTATTGGTAGGGGATTTTATATATACACGAGCCTTTCAAATGATGACTGAATTAGAATCCTTGCGAATATTATCTT
TGATGGCAGATGCAGTCAATATAATTGCAGCAGGTGAGATATTGCAATTAACACATTGTAATGACCCCACTATTACTATC
GATAGTTACATGAAAATTATTTATAGTAAAACTGCTCGTTTATTTGAAGTAGCTTCTCAATCATCTGCTATTTTAGCTGA
TGCCAATACATGTCAAGAAAAAGCATTACGTAATTATGGGCGATATATGGGGATTGCCTTTCAACTAGTTGATGATGTAT
TAGATTATTCTGCCTCAGAAACAATATTTGGGAAAAATGTCGGAAATGATTTAAATGAAGGAAAGCTTACTCTTCCTCTG
CTACACGCTATTCATCATAGCACCTCAAAACAAGCATCACTTATAATTCAATCTATTAAAGAAGGTAACCATCGTCACTT
GTTAAATATAATTTTAGATACAATGCATCAACATGGATCATTAGAATACACTCGAAAACGCGCTGAAACAGAAATCAAAA
AAGCTATCTCTTGTCTTGATATTTTACCTCCTTCTCCTTATCGACAAGCATTAGCAAGTTTAGCGACTTATACGGTTCAA
AGAATTTATTAG

Upstream 100 bases:

>100_bases
AATTTACTGCATACTCATAAGTGTAGTTTAACTTAATAAAAAAATTGCGTACGTAAACAATATATAAATACATCAACATT
TTATATTTATAATAATATTA

Downstream 100 bases:

>100_bases
ATTTTCATCTTTAAAATCACAAATAATTTGACATTAAAATTATATATAAAATATAAAATTGTGATGCTTATAAACATGAA
ATACTTTTATTAACTGTAAT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNIDQISELTEQDMEDVNSEIRTRLASEITLINKLVQYIVDSGGKRIRPMITLLTARALCYKKTQHVTIATLIEFIHTAT
LLHDDVVDKSHMRRGRKTTNIIFGNAASVLVGDFIYTRAFQMMTELESLRILSLMADAVNIIAAGEILQLTHCNDPTITI
DSYMKIIYSKTARLFEVASQSSAILADANTCQEKALRNYGRYMGIAFQLVDDVLDYSASETIFGKNVGNDLNEGKLTLPL
LHAIHHSTSKQASLIIQSIKEGNHRHLLNIILDTMHQHGSLEYTRKRAETEIKKAISCLDILPPSPYRQALASLATYTVQ
RIY

Sequences:

>Translated_323_residues
MNIDQISELTEQDMEDVNSEIRTRLASEITLINKLVQYIVDSGGKRIRPMITLLTARALCYKKTQHVTIATLIEFIHTAT
LLHDDVVDKSHMRRGRKTTNIIFGNAASVLVGDFIYTRAFQMMTELESLRILSLMADAVNIIAAGEILQLTHCNDPTITI
DSYMKIIYSKTARLFEVASQSSAILADANTCQEKALRNYGRYMGIAFQLVDDVLDYSASETIFGKNVGNDLNEGKLTLPL
LHAIHHSTSKQASLIIQSIKEGNHRHLLNIILDTMHQHGSLEYTRKRAETEIKKAISCLDILPPSPYRQALASLATYTVQ
RIY
>Mature_323_residues
MNIDQISELTEQDMEDVNSEIRTRLASEITLINKLVQYIVDSGGKRIRPMITLLTARALCYKKTQHVTIATLIEFIHTAT
LLHDDVVDKSHMRRGRKTTNIIFGNAASVLVGDFIYTRAFQMMTELESLRILSLMADAVNIIAAGEILQLTHCNDPTITI
DSYMKIIYSKTARLFEVASQSSAILADANTCQEKALRNYGRYMGIAFQLVDDVLDYSASETIFGKNVGNDLNEGKLTLPL
LHAIHHSTSKQASLIIQSIKEGNHRHLLNIILDTMHQHGSLEYTRKRAETEIKKAISCLDILPPSPYRQALASLATYTVQ
RIY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=321, Percent_Identity=29.595015576324, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4758430, Length=273, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI83700220, Length=273, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=62.3052959501558, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786623, Length=248, Percent_Identity=29.0322580645161, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=303, Percent_Identity=30.6930693069307, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=323, Percent_Identity=30.030959752322, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=230, Percent_Identity=26.0869565217391, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651612, Length=325, Percent_Identity=28.9230769230769, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281365769, Length=275, Percent_Identity=23.6363636363636, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24660002, Length=275, Percent_Identity=23.6363636363636, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36295; Mature: 36295

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDQISELTEQDMEDVNSEIRTRLASEITLINKLVQYIVDSGGKRIRPMITLLTARALC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YKKTQHVTIATLIEFIHTATLLHDDVVDKSHMRRGRKTTNIIFGNAASVLVGDFIYTRAF
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
QMMTELESLRILSLMADAVNIIAAGEILQLTHCNDPTITIDSYMKIIYSKTARLFEVASQ
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCC
SSAILADANTCQEKALRNYGRYMGIAFQLVDDVLDYSASETIFGKNVGNDLNEGKLTLPL
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
LHAIHHSTSKQASLIIQSIKEGNHRHLLNIILDTMHQHGSLEYTRKRAETEIKKAISCLD
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPPSPYRQALASLATYTVQRIY
CCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIDQISELTEQDMEDVNSEIRTRLASEITLINKLVQYIVDSGGKRIRPMITLLTARALC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YKKTQHVTIATLIEFIHTATLLHDDVVDKSHMRRGRKTTNIIFGNAASVLVGDFIYTRAF
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHH
QMMTELESLRILSLMADAVNIIAAGEILQLTHCNDPTITIDSYMKIIYSKTARLFEVASQ
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCC
SSAILADANTCQEKALRNYGRYMGIAFQLVDDVLDYSASETIFGKNVGNDLNEGKLTLPL
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
LHAIHHSTSKQASLIIQSIKEGNHRHLLNIILDTMHQHGSLEYTRKRAETEIKKAISCLD
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPPSPYRQALASLATYTVQRIY
CCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]