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The map label for this gene is dnaB [H]

Identifier: 71891819

GI number: 71891819

Start: 32173

End: 33588

Strand: Direct

Name: dnaB [H]

Synonym: BPEN_027

Alternate gene names: 71891819

Gene position: 32173-33588 (Clockwise)

Preceding gene: 71891817

Following gene: 71891820

Centisome position: 4.06

GC content: 33.69

Gene sequence:

>1416_bases
TTGTTAAGGAAAATAGAAATTTATGCGGGGAAAATATTAAAAATGCATGTTTCTCATGATCAGCAAGTAAATAATATGAA
AATACCACCGTATTCATTAGAAGCTGAACAATCAATGTTAGGTGGTTTAATGTTAGATAATACTCAATGGGAATATATAT
CAGTACAAATAAATTCTGATGATTTTTTTAATTATGCTCATAGAATTATTTTTACTGAAATGAAACGTTTACTGGAAACA
AATAAACCTATTGACTTAATAACACTGTCAGAATCTTTAGAGATTCAAGGAAAATTGGAATTAGTAGGAGGATTTGCGTA
TTTAGCAGAATTATCAAAGAATGTTCCCAGTGCTTCTAATGTAGTTGCATATGCAGATATAGTACGCGAACGATCAGTAA
TACGCGCAATGATTTCTATAGCAAATAAAATTGCTGATGCCGGATATAATCCTCAAGGCCGAAGCAGTGATGAGCTATTA
GATTTAGCTGAATCTCTCATTTTTCAAATTTCACATAGTAAAAGCAATCATAATATTAATCCAAAAGGAATAGACCATAT
TTTAGAAAAGACAGTCGCTCATATTGAGCAGGTATGTAATAGACCAAAATATGGTGTTACTGGTGTTTCTAGCGGATATA
GAGACTTGGATAAAAAAACTGATGGTTTACAAAAATCTGATCTTATAATTATTGCCGCTCGCCCTTCTATGGGAAAAACT
GCTTTTGCTATGAATTTGTGTGAACATGCCGCAATGACTGAAAAAAAACCAGTATTGATATTTAGTTTAGAAATGCCTGG
CGATCAAATTATGATACGTATGTTAGCTTCATTATCTCGAGTTGATCAAGTACGTATTCGCACGGGACGACTGAATAATG
AAGACCGAGAAAGAATTACTAGTGCTATGGAACTTCTTTTGGAAAAGCGTAATATATATATTGATGATTCCTCGTGTTTA
ACACCTGCTGAAGTGCGTGGACGGGCTCGTAGGTTGTTCCGTGAACATGATGGTTTGAGTTTAATTATGATTGATTATTT
ACAATTAATGAGAGTACCGTCTTTATCTAATAATCGCACATTAGAAATTTCAGAAATTTCTCGATCGTTAAAAGCTTTAG
CTAAGGAATTGAAAGTACCAGTCATAGCGATTTCTCAGTTAAACAGAGGTTTAGAACAACGCACTGACAAGCATCCCGTT
AACTCAGATTTGCGTGAATCCGGAGCAATTGAACAAGATGCTGATCTTATTTTATTTATTTATCGTGATGAAGTGTATCA
TGAAAACAGTGAAATGAAAGGGATTGCTGAAATTATTCTAGGGAAACAACGGAATGGTCCCATTGGCACTATACGATTGA
TGTTTAACGGAAAATGTTCTCGATTTGATAACTATGTTAAATCTGGTCAATATTAA

Upstream 100 bases:

>100_bases
TATGGTTTTATGATTTATATCAGTGTAATTATTTTAGGTGTTTTATATGGTATTGTAATAGTGGAAATTAAATTTTTCGT
GTTTAAAATTTAGATATTTC

Downstream 100 bases:

>100_bases
TATTCTATTAGTGATATGGATTGGTTATAGATATTATTATATGAAATAATTTATGTATTTATTCTATGATAAAGTTTTGG
TATCTGTTTTATAAAAAATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLRKIEIYAGKILKMHVSHDQQVNNMKIPPYSLEAEQSMLGGLMLDNTQWEYISVQINSDDFFNYAHRIIFTEMKRLLET
NKPIDLITLSESLEIQGKLELVGGFAYLAELSKNVPSASNVVAYADIVRERSVIRAMISIANKIADAGYNPQGRSSDELL
DLAESLIFQISHSKSNHNINPKGIDHILEKTVAHIEQVCNRPKYGVTGVSSGYRDLDKKTDGLQKSDLIIIAARPSMGKT
AFAMNLCEHAAMTEKKPVLIFSLEMPGDQIMIRMLASLSRVDQVRIRTGRLNNEDRERITSAMELLLEKRNIYIDDSSCL
TPAEVRGRARRLFREHDGLSLIMIDYLQLMRVPSLSNNRTLEISEISRSLKALAKELKVPVIAISQLNRGLEQRTDKHPV
NSDLRESGAIEQDADLILFIYRDEVYHENSEMKGIAEIILGKQRNGPIGTIRLMFNGKCSRFDNYVKSGQY

Sequences:

>Translated_471_residues
MLRKIEIYAGKILKMHVSHDQQVNNMKIPPYSLEAEQSMLGGLMLDNTQWEYISVQINSDDFFNYAHRIIFTEMKRLLET
NKPIDLITLSESLEIQGKLELVGGFAYLAELSKNVPSASNVVAYADIVRERSVIRAMISIANKIADAGYNPQGRSSDELL
DLAESLIFQISHSKSNHNINPKGIDHILEKTVAHIEQVCNRPKYGVTGVSSGYRDLDKKTDGLQKSDLIIIAARPSMGKT
AFAMNLCEHAAMTEKKPVLIFSLEMPGDQIMIRMLASLSRVDQVRIRTGRLNNEDRERITSAMELLLEKRNIYIDDSSCL
TPAEVRGRARRLFREHDGLSLIMIDYLQLMRVPSLSNNRTLEISEISRSLKALAKELKVPVIAISQLNRGLEQRTDKHPV
NSDLRESGAIEQDADLILFIYRDEVYHENSEMKGIAEIILGKQRNGPIGTIRLMFNGKCSRFDNYVKSGQY
>Mature_471_residues
MLRKIEIYAGKILKMHVSHDQQVNNMKIPPYSLEAEQSMLGGLMLDNTQWEYISVQINSDDFFNYAHRIIFTEMKRLLET
NKPIDLITLSESLEIQGKLELVGGFAYLAELSKNVPSASNVVAYADIVRERSVIRAMISIANKIADAGYNPQGRSSDELL
DLAESLIFQISHSKSNHNINPKGIDHILEKTVAHIEQVCNRPKYGVTGVSSGYRDLDKKTDGLQKSDLIIIAARPSMGKT
AFAMNLCEHAAMTEKKPVLIFSLEMPGDQIMIRMLASLSRVDQVRIRTGRLNNEDRERITSAMELLLEKRNIYIDDSSCL
TPAEVRGRARRLFREHDGLSLIMIDYLQLMRVPSLSNNRTLEISEISRSLKALAKELKVPVIAISQLNRGLEQRTDKHPV
NSDLRESGAIEQDADLILFIYRDEVYHENSEMKGIAEIILGKQRNGPIGTIRLMFNGKCSRFDNYVKSGQY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=448, Percent_Identity=70.9821428571429, Blast_Score=669, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53283; Mature: 53283

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKIEIYAGKILKMHVSHDQQVNNMKIPPYSLEAEQSMLGGLMLDNTQWEYISVQINSD
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCEEEEEEEECCC
DFFNYAHRIIFTEMKRLLETNKPIDLITLSESLEIQGKLELVGGFAYLAELSKNVPSASN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCCCC
VVAYADIVRERSVIRAMISIANKIADAGYNPQGRSSDELLDLAESLIFQISHSKSNHNIN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PKGIDHILEKTVAHIEQVCNRPKYGVTGVSSGYRDLDKKTDGLQKSDLIIIAARPSMGKT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCH
AFAMNLCEHAAMTEKKPVLIFSLEMPGDQIMIRMLASLSRVDQVRIRTGRLNNEDRERIT
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
SAMELLLEKRNIYIDDSSCLTPAEVRGRARRLFREHDGLSLIMIDYLQLMRVPSLSNNRT
HHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCE
LEISEISRSLKALAKELKVPVIAISQLNRGLEQRTDKHPVNSDLRESGAIEQDADLILFI
EEHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEE
YRDEVYHENSEMKGIAEIILGKQRNGPIGTIRLMFNGKCSRFDNYVKSGQY
EECHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLRKIEIYAGKILKMHVSHDQQVNNMKIPPYSLEAEQSMLGGLMLDNTQWEYISVQINSD
CCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEECCCCEEEEEEEECCC
DFFNYAHRIIFTEMKRLLETNKPIDLITLSESLEIQGKLELVGGFAYLAELSKNVPSASN
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCCCC
VVAYADIVRERSVIRAMISIANKIADAGYNPQGRSSDELLDLAESLIFQISHSKSNHNIN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PKGIDHILEKTVAHIEQVCNRPKYGVTGVSSGYRDLDKKTDGLQKSDLIIIAARPSMGKT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCH
AFAMNLCEHAAMTEKKPVLIFSLEMPGDQIMIRMLASLSRVDQVRIRTGRLNNEDRERIT
HHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
SAMELLLEKRNIYIDDSSCLTPAEVRGRARRLFREHDGLSLIMIDYLQLMRVPSLSNNRT
HHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCE
LEISEISRSLKALAKELKVPVIAISQLNRGLEQRTDKHPVNSDLRESGAIEQDADLILFI
EEHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEE
YRDEVYHENSEMKGIAEIILGKQRNGPIGTIRLMFNGKCSRFDNYVKSGQY
EECHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]