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The map label for this gene is trmE

Identifier: 71891804

GI number: 71891804

Start: 10913

End: 12325

Strand: Reverse

Name: trmE

Synonym: BPEN_011

Alternate gene names: 71891804

Gene position: 12325-10913 (Counterclockwise)

Preceding gene: 71891805

Following gene: NA

Centisome position: 1.56

GC content: 31.21

Gene sequence:

>1413_bases
ATGAACTATACAATAGATACAGTGGTTGCTATTTCCACACCTCCAGGCCGTGGAGGTATTGGAATTATACGAATATCTGG
AAAATCAGTTCCTGAAATTGCTCCTAAATTGTTAGGAAAAATACCAAATCCCAGAAAAGCAGAATATTTACCTTTTTTGG
ACACGGACGGATCCATATTAGAACGAGTGATAGCCTTGTTTTTTCCTGAACCAAATTCTTTTACCGGGGAAAACATACTA
GAAATCCATGGACATGGAGGTCAAATAATCCTAGATATATTATTGGAACGTATTTTAAAAACATCTTCTGATATTCGAAT
AGCACATCCAGGAGAATTTACCAAACGAGCATTCTTAAATAACAAAATAGATCTTGTACAAGCAGAAGCAATAGCTGATA
TTATCGATGCTACTTCATATCAGGCTGCAAAATCAGCATCAAATTCTTTACAAGGAATTTTCTCTCGTAAAATATATATA
ATATTAGAACAACTTACTAATCTACGCATGTACGCAGAATCATCTATTGACTTTTCAGAAGACGAAATTAGTATCATACC
ATATGAAGATATAAAAAAAAAATTGAGGAACATCATAAGCGATGTACAAAAAATGTATAAATCAACCTATCACGGTGTTT
TACTCCGTGAAGGGATAAAAATAGTAATTGCTGGAAAACCAAATGCTGGAAAATCTAGTTTATTTAATGCATTAGTTGGC
ATTGATAGAGCAATTATCAGTACGATATCCGGAACCACACGAGATACATTGCATGAGTATATCCAATTAAATGGAATAGC
ATTCCATATTACTGACACTGCTGGGCTACAAAAAAAAAGCGATAACGAAATAGAACAAATTGGGATGAAACGTACATGGG
AAGAATTAAGTAACGCAGATCATATATTATGGGTAATTGATCCTAACGATGTAACAAATAAGGAAAATGATATCACATTA
AAACATGTAGAAAAAGTGCTGTTTTGTAAAAATAAAAAAACCCCTATCACAATTATACATAATAAATCTGATTTAACCAA
AAATCAAATCGGAATTAGTATAATAAATAATTATACAATTATCACGTTATCGGCTTTATTTAATGATGGAACAGACTTAT
TACAAGAATATTTAAGTAATAATATCAAGTCTAAAATACAGCAAGATTGCAAAAGCAATCTTTCTGAAAATCAAGGAAAT
TTCATTGCGCGTAGACGTCACTTAAATGCACTTGAAAAATCTTCTAAATACCTTTTTTCTGCCCAAACACAACTATTATC
TACTATGTCAATTAATGAACTTTTTGCTGAAGATCTTGGATTAGCACATAAAGAATTAAGCAAAATTTTTGGAAAATTTA
CTCCTGATGATTTATTAAAAAGAATATTCTCTACATTCTGTATTGGAAAATGA

Upstream 100 bases:

>100_bases
GAGGATTATACAATAAAAATAACTAATAAAAATATTCAAAAAAATTTATAACTCAAGTATATCTTCGCATTATAATATGT
TTTAAAGTATAATAAAAATT

Downstream 100 bases:

>100_bases
TGATTTATGAACTTATAAATAATTTTTATAATATTTTATAAATATAAATTTCAAGAATGAAATCGTTTTTTTTTGCTATA
ATTACATATAGGTTATAGAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNYTIDTVVAISTPPGRGGIGIIRISGKSVPEIAPKLLGKIPNPRKAEYLPFLDTDGSILERVIALFFPEPNSFTGENIL
EIHGHGGQIILDILLERILKTSSDIRIAHPGEFTKRAFLNNKIDLVQAEAIADIIDATSYQAAKSASNSLQGIFSRKIYI
ILEQLTNLRMYAESSIDFSEDEISIIPYEDIKKKLRNIISDVQKMYKSTYHGVLLREGIKIVIAGKPNAGKSSLFNALVG
IDRAIISTISGTTRDTLHEYIQLNGIAFHITDTAGLQKKSDNEIEQIGMKRTWEELSNADHILWVIDPNDVTNKENDITL
KHVEKVLFCKNKKTPITIIHNKSDLTKNQIGISIINNYTIITLSALFNDGTDLLQEYLSNNIKSKIQQDCKSNLSENQGN
FIARRRHLNALEKSSKYLFSAQTQLLSTMSINELFAEDLGLAHKELSKIFGKFTPDDLLKRIFSTFCIGK

Sequences:

>Translated_470_residues
MNYTIDTVVAISTPPGRGGIGIIRISGKSVPEIAPKLLGKIPNPRKAEYLPFLDTDGSILERVIALFFPEPNSFTGENIL
EIHGHGGQIILDILLERILKTSSDIRIAHPGEFTKRAFLNNKIDLVQAEAIADIIDATSYQAAKSASNSLQGIFSRKIYI
ILEQLTNLRMYAESSIDFSEDEISIIPYEDIKKKLRNIISDVQKMYKSTYHGVLLREGIKIVIAGKPNAGKSSLFNALVG
IDRAIISTISGTTRDTLHEYIQLNGIAFHITDTAGLQKKSDNEIEQIGMKRTWEELSNADHILWVIDPNDVTNKENDITL
KHVEKVLFCKNKKTPITIIHNKSDLTKNQIGISIINNYTIITLSALFNDGTDLLQEYLSNNIKSKIQQDCKSNLSENQGN
FIARRRHLNALEKSSKYLFSAQTQLLSTMSINELFAEDLGLAHKELSKIFGKFTPDDLLKRIFSTFCIGK
>Mature_470_residues
MNYTIDTVVAISTPPGRGGIGIIRISGKSVPEIAPKLLGKIPNPRKAEYLPFLDTDGSILERVIALFFPEPNSFTGENIL
EIHGHGGQIILDILLERILKTSSDIRIAHPGEFTKRAFLNNKIDLVQAEAIADIIDATSYQAAKSASNSLQGIFSRKIYI
ILEQLTNLRMYAESSIDFSEDEISIIPYEDIKKKLRNIISDVQKMYKSTYHGVLLREGIKIVIAGKPNAGKSSLFNALVG
IDRAIISTISGTTRDTLHEYIQLNGIAFHITDTAGLQKKSDNEIEQIGMKRTWEELSNADHILWVIDPNDVTNKENDITL
KHVEKVLFCKNKKTPITIIHNKSDLTKNQIGISIINNYTIITLSALFNDGTDLLQEYLSNNIKSKIQQDCKSNLSENQGN
FIARRRHLNALEKSSKYLFSAQTQLLSTMSINELFAEDLGLAHKELSKIFGKFTPDDLLKRIFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=480, Percent_Identity=31.0416666666667, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI193082988, Length=510, Percent_Identity=29.0196078431373, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI193082993, Length=477, Percent_Identity=29.979035639413, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=50, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI87082120, Length=128, Percent_Identity=29.6875, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=475, Percent_Identity=27.1578947368421, Blast_Score=179, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=508, Percent_Identity=32.6771653543307, Blast_Score=207, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=27.6288659793814, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BLOPB (Q494C0)

Other databases:

- EMBL:   CP000016
- RefSeq:   YP_277533.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q494C0
- SMR:   Q494C0
- STRING:   Q494C0
- GeneID:   3562626
- GenomeReviews:   CP000016_GR
- KEGG:   bpn:BPEN_011
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   CBLO291272:BPEN_011-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 52561; Mature: 52561

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 81-81 BINDING 122-122 BINDING 470-470

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTIDTVVAISTPPGRGGIGIIRISGKSVPEIAPKLLGKIPNPRKAEYLPFLDTDGSIL
CCEEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEECCCHHHH
ERVIALFFPEPNSFTGENILEIHGHGGQIILDILLERILKTSSDIRIAHPGEFTKRAFLN
HHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHC
NKIDLVQAEAIADIIDATSYQAAKSASNSLQGIFSRKIYIILEQLTNLRMYAESSIDFSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEISIIPYEDIKKKLRNIISDVQKMYKSTYHGVLLREGIKIVIAGKPNAGKSSLFNALVG
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHH
IDRAIISTISGTTRDTLHEYIQLNGIAFHITDTAGLQKKSDNEIEQIGMKRTWEELSNAD
HHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
HILWVIDPNDVTNKENDITLKHVEKVLFCKNKKTPITIIHNKSDLTKNQIGISIINNYTI
EEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCEEEECCEEE
ITLSALFNDGTDLLQEYLSNNIKSKIQQDCKSNLSENQGNFIARRRHLNALEKSSKYLFS
EEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQTQLLSTMSINELFAEDLGLAHKELSKIFGKFTPDDLLKRIFSTFCIGK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNYTIDTVVAISTPPGRGGIGIIRISGKSVPEIAPKLLGKIPNPRKAEYLPFLDTDGSIL
CCEEEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEECCCHHHH
ERVIALFFPEPNSFTGENILEIHGHGGQIILDILLERILKTSSDIRIAHPGEFTKRAFLN
HHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHC
NKIDLVQAEAIADIIDATSYQAAKSASNSLQGIFSRKIYIILEQLTNLRMYAESSIDFSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEISIIPYEDIKKKLRNIISDVQKMYKSTYHGVLLREGIKIVIAGKPNAGKSSLFNALVG
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCCCHHHHHHHHHH
IDRAIISTISGTTRDTLHEYIQLNGIAFHITDTAGLQKKSDNEIEQIGMKRTWEELSNAD
HHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
HILWVIDPNDVTNKENDITLKHVEKVLFCKNKKTPITIIHNKSDLTKNQIGISIINNYTI
EEEEEECCCCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCEEEECCEEE
ITLSALFNDGTDLLQEYLSNNIKSKIQQDCKSNLSENQGNFIARRRHLNALEKSSKYLFS
EEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AQTQLLSTMSINELFAEDLGLAHKELSKIFGKFTPDDLLKRIFSTFCIGK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA