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The map label for this gene is mnhD [H]

Identifier: 68537083

GI number: 68537083

Start: 2318690

End: 2320381

Strand: Direct

Name: mnhD [H]

Synonym: jk1988

Alternate gene names: 68537083

Gene position: 2318690-2320381 (Clockwise)

Preceding gene: 68537082

Following gene: 68537084

Centisome position: 94.16

GC content: 64.78

Gene sequence:

>1692_bases
ATGACCGAAGGTTCCCTACTCAGCCTGTTTATTGCCGTCCCGCTGCTTTCCGCGGCCGTCGCCGCGATGGCTCCGTGGAT
GAGCCTCCGGCGTGCGTTGGGTCTACTGGTGCCAATCTTCGGCATTGCCGGCAGCATCTGGATGCTGGTGCAGGTCAACG
AGCCGAACACGGTGCTGGTCGATAACGTCGGCGGGTTCGTCGGCGGTATCTCCATCCCGCTGGTCGCCGACAATCTGGCG
GGCGTCATGATCCTGACGACCTCCGTCGTCGCCCTGATCGCCAACTGGTTCGCCGACGTCGTCGGCGAGACGCGCACCCG
CTTCTACCCCGCCCTCACGCTCATGCTGATCGGCGGCGTGTGGGGTGCGCTGCTGACCGCGGACTTGTTCAACCTGTTCG
TGTTCATCGAGGTCATGCTGATGCCCTCCTTCGGCCTGCTGGCGATGACCGGCACGTGGGCTCGCCTGGCGGCGGGCAGG
ATGTTCATCATCGTGAACCTGGTGACCTCCCTAGTGCTGCTGAGTGGCGTGGTACTGGTCTACGCCGTGGTGGGCACCAC
CAACCTCGCCGCCCTGGCGGGTGCAGCCGGACCCCGTGGCACCGAGTTTGCCGAAGGAGTCTTCGGCAACCAGTGGCAGC
TAGTGGTCCCGCTGGGCGTGGTTCTGCTGGCGCTGGCCGTGAAGGCGGGTGTGGCTCCGGTCCACACCTGGCTGCCGCGT
GCCTACCCGTCTACTTCCCCGGCCGTGATGGCGCTGTTCTCCGGCCTGCACACCAAGGTCGGCATCTACGCGATTTTCCG
CGTGTACCTGACCATCTTCGAAGGCGACCCCACTTGGTCCTGGGCAATCCTGGTGATCGTAGTGCTGGGCATGCTGATCG
GCGGCTTCGCCGGCCTGGGCGAAACCACGATGCGCAGCGTGATCGCCTACCAGATGGTCAACGGCATCCCATTCATGCTG
ATTGGACTGGCTTTCCTCAGCAACGATGCCCGTCTCATGCTCAGCGCAGCACTGTTCTACATGCTGCACCACATGATTGT
CGCCGGATCCCTCATCATGGCCTCCGGCGCGATCGAGGAGACCTACGGTTCGGGCCGCGTGCGCAAGCTCTCCGGGCTGA
TGCGCCGCGACCGCTTCGTCGCTGTCGTCTTCGCGGCGGGTGCGCTGGCGATCGTTGGCCTGCCGCCGTTCTCCGGCCTG
TGGGGCAAGCTGGCGCTAGTCTTCGGCGTGGCCCAGGACGGCACGTGGAAGACCTGGTTGGCCATCGGCGCGATCATCGT
TTCTTCCATCGGCGCTCTGGTCTCCATGATCTACGTGTGGCGCGAGGTGTTCTGGGGCCGTCAGATGAACGCCAACGAGG
CCGACCCGGAGCTGCGCGTACCGCGCCGCTACGTGTGGCCGTCCGCAGTCATGATGCTGATTTCCGTGGGCATGTTCATC
GGCGCCGGGCCGGTCTTCCAGCAGGTCAACAAGGCCGCCGATAACCTGACCGACACGACCGCTTATGTCGAGGCAGTCCT
CGGCGATCCGGATGAGGCCGTCGGCGCGGTCCTGCCGCCCGGCCCGAGCGGATTGGATAATGTTCCTGCCGGTGCGCGTA
GTACTACGAACAATGACGCCCACACCGAGCGTGCTGAGCGTGCCGAACGCGAAAGCAAGGAACAGCAGGCTCAGGACGGG
AAGGATCGCTAA

Upstream 100 bases:

>100_bases
CGGAGGTCCACTCCACCGCCGACGAGGACGGGCCGGGCGAGGCCGCGGCTCACAATACGGGCAAAGCGCGTGCAAACGAT
CAGAAGGGAGCTCAGAAGTA

Downstream 100 bases:

>100_bases
GCCATGGCAAAGTTCAGTATTGTCCAAGCAGTGAAGTCCACCGGCCACGCGCTGAAGTACGCCGCCTGGCTGGTCGGGCA
GATCATCAAGGAGTCGGTCG

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTEGSLLSLFIAVPLLSAAVAAMAPWMSLRRALGLLVPIFGIAGSIWMLVQVNEPNTVLVDNVGGFVGGISIPLVADNLA
GVMILTTSVVALIANWFADVVGETRTRFYPALTLMLIGGVWGALLTADLFNLFVFIEVMLMPSFGLLAMTGTWARLAAGR
MFIIVNLVTSLVLLSGVVLVYAVVGTTNLAALAGAAGPRGTEFAEGVFGNQWQLVVPLGVVLLALAVKAGVAPVHTWLPR
AYPSTSPAVMALFSGLHTKVGIYAIFRVYLTIFEGDPTWSWAILVIVVLGMLIGGFAGLGETTMRSVIAYQMVNGIPFML
IGLAFLSNDARLMLSAALFYMLHHMIVAGSLIMASGAIEETYGSGRVRKLSGLMRRDRFVAVVFAAGALAIVGLPPFSGL
WGKLALVFGVAQDGTWKTWLAIGAIIVSSIGALVSMIYVWREVFWGRQMNANEADPELRVPRRYVWPSAVMMLISVGMFI
GAGPVFQQVNKAADNLTDTTAYVEAVLGDPDEAVGAVLPPGPSGLDNVPAGARSTTNNDAHTERAERAERESKEQQAQDG
KDR

Sequences:

>Translated_563_residues
MTEGSLLSLFIAVPLLSAAVAAMAPWMSLRRALGLLVPIFGIAGSIWMLVQVNEPNTVLVDNVGGFVGGISIPLVADNLA
GVMILTTSVVALIANWFADVVGETRTRFYPALTLMLIGGVWGALLTADLFNLFVFIEVMLMPSFGLLAMTGTWARLAAGR
MFIIVNLVTSLVLLSGVVLVYAVVGTTNLAALAGAAGPRGTEFAEGVFGNQWQLVVPLGVVLLALAVKAGVAPVHTWLPR
AYPSTSPAVMALFSGLHTKVGIYAIFRVYLTIFEGDPTWSWAILVIVVLGMLIGGFAGLGETTMRSVIAYQMVNGIPFML
IGLAFLSNDARLMLSAALFYMLHHMIVAGSLIMASGAIEETYGSGRVRKLSGLMRRDRFVAVVFAAGALAIVGLPPFSGL
WGKLALVFGVAQDGTWKTWLAIGAIIVSSIGALVSMIYVWREVFWGRQMNANEADPELRVPRRYVWPSAVMMLISVGMFI
GAGPVFQQVNKAADNLTDTTAYVEAVLGDPDEAVGAVLPPGPSGLDNVPAGARSTTNNDAHTERAERAERESKEQQAQDG
KDR
>Mature_562_residues
TEGSLLSLFIAVPLLSAAVAAMAPWMSLRRALGLLVPIFGIAGSIWMLVQVNEPNTVLVDNVGGFVGGISIPLVADNLAG
VMILTTSVVALIANWFADVVGETRTRFYPALTLMLIGGVWGALLTADLFNLFVFIEVMLMPSFGLLAMTGTWARLAAGRM
FIIVNLVTSLVLLSGVVLVYAVVGTTNLAALAGAAGPRGTEFAEGVFGNQWQLVVPLGVVLLALAVKAGVAPVHTWLPRA
YPSTSPAVMALFSGLHTKVGIYAIFRVYLTIFEGDPTWSWAILVIVVLGMLIGGFAGLGETTMRSVIAYQMVNGIPFMLI
GLAFLSNDARLMLSAALFYMLHHMIVAGSLIMASGAIEETYGSGRVRKLSGLMRRDRFVAVVFAAGALAIVGLPPFSGLW
GKLALVFGVAQDGTWKTWLAIGAIIVSSIGALVSMIYVWREVFWGRQMNANEADPELRVPRRYVWPSAVMMLISVGMFIG
AGPVFQQVNKAADNLTDTTAYVEAVLGDPDEAVGAVLPPGPSGLDNVPAGARSTTNNDAHTERAERAERESKEQQAQDGK
DR

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=363, Percent_Identity=26.9972451790634, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1788827, Length=293, Percent_Identity=28.3276450511945, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788829, Length=353, Percent_Identity=22.6628895184136, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788831, Length=265, Percent_Identity=25.6603773584906, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI145693160, Length=447, Percent_Identity=24.3847874720358, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788614, Length=389, Percent_Identity=23.6503856041131, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI2367154, Length=183, Percent_Identity=26.775956284153, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 59886; Mature: 59755

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGSLLSLFIAVPLLSAAVAAMAPWMSLRRALGLLVPIFGIAGSIWMLVQVNEPNTVLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEE
DNVGGFVGGISIPLVADNLAGVMILTTSVVALIANWFADVVGETRTRFYPALTLMLIGGV
ECCCCHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGALLTADLFNLFVFIEVMLMPSFGLLAMTGTWARLAAGRMFIIVNLVTSLVLLSGVVLV
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YAVVGTTNLAALAGAAGPRGTEFAEGVFGNQWQLVVPLGVVLLALAVKAGVAPVHTWLPR
HHHHCCCHHHHHHCCCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHCCC
AYPSTSPAVMALFSGLHTKVGIYAIFRVYLTIFEGDPTWSWAILVIVVLGMLIGGFAGLG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
ETTMRSVIAYQMVNGIPFMLIGLAFLSNDARLMLSAALFYMLHHMIVAGSLIMASGAIEE
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
TYGSGRVRKLSGLMRRDRFVAVVFAAGALAIVGLPPFSGLWGKLALVFGVAQDGTWKTWL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHEECCCCCHHHHH
AIGAIIVSSIGALVSMIYVWREVFWGRQMNANEADPELRVPRRYVWPSAVMMLISVGMFI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHH
GAGPVFQQVNKAADNLTDTTAYVEAVLGDPDEAVGAVLPPGPSGLDNVPAGARSTTNNDA
CCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
HTERAERAERESKEQQAQDGKDR
HHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TEGSLLSLFIAVPLLSAAVAAMAPWMSLRRALGLLVPIFGIAGSIWMLVQVNEPNTVLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEE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HHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]