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The map label for this gene is matE [H]

Identifier: 68537077

GI number: 68537077

Start: 2310046

End: 2311425

Strand: Direct

Name: matE [H]

Synonym: jk1982

Alternate gene names: 68537077

Gene position: 2310046-2311425 (Clockwise)

Preceding gene: 68537076

Following gene: 68537078

Centisome position: 93.81

GC content: 61.74

Gene sequence:

>1380_bases
ATGAGGGTGGCTGCGAGGTTGAACTCCATTCAGAAGTACCGCGAAGTCCTTGGAATTTCCCTTCCCATCGCCGGGGTGCA
GCTCGCCGGGGTGGCGCTGACCACCACGGACGTCCTGATGCTGCAATCCGTGGGGGTGCTGGCGATCGCTGGCGGTGGCT
TGGCCATGCAGCTATACAACCAGATCCGCACGATGTGCGTGGGCATGGTGACGGCCAGCTCAAATCTCGTGGCCGAGGCT
GCGGCAGAGATGGAACGGGAAGAAGCCTCTAGTCAGCCCTCGGAGGAAGGCGCGGAGCGGATCCGGCGAGCCGTGCGATC
CTCCTTCGCCGTGGGCACAGGCACTGCGGTCCTCGGCGCGCTACTGGTCATCACCTTCGGCGCACTGGTTCTTGCTCTGC
CTATCGACGAGCAGGTCGCCCATCTCACCTTCGCCATGACCCTCACATTGGCGCCGGGACTCATCCCCATGTTGTGGCTC
AACGTCTTGCGCCAGTTCGCGGTGGGCATGCGCAAGCCTGGCTCCCTGCTTGCCATCACGCTGGTATCAATCTTCGTCAA
CATCGCCCTGAACTGGTCGTTTATGGCTTGGGCAACGTCGGACACATGGAAGGTGGCGGGCATCGGGCTATCCACGACCA
CCGTCCAACTTTTCACGTTGCTAGCCTTCCGCGTGGTCGTGCTGCGTATACCCGAGCTGCAACCCTACTTTCAGGTTCTG
CCCCGCGCCGGCGACGGGGAGACTATACGCCACCTCGTGCGCCTCGGAGTACCAGTTAGCCTGACCTATGGCTCGGAGGC
AGCGATTACGACAATCGCCGGGTTGGCGATGGGAACCCTCAGCCCACAGATGTTGGCAGCGCACAACGTTGTCAACCAGC
TAGCGTACATCGTCTACCAGGTTTGCATCGGCTTCTCCCATGGCGGGTCGGTGCTCATCACGAGGGCACGAGCCGAAGGC
GCCAACGCGGTGCGCGCGGTAACCCGGCGCGTGCTACTCGTCGTGGTCGTGTACCTGGGGATTATGGGCGCACTGTGGCT
TCTGCTGGGTCGCTGGATACTGCGCCCGTTCCTGGGAAGTGCAGACGCCACCACCTTCCACATCGCCGTCCTGCTTCTGT
GCCTCGCCGTGCTTCAGCAGTTCTCTAAGGGAATTCAGAACGTGACAGTGGGTTTGCTGAGGGGGCTCAAGGACACCACT
TCCGGGCTGCGCTGCACTCTCATTGGATATTGGCTAGTCGGCGTACCCGGAATTCTGGTGATGGGCAAGCTCCTTAGGTG
GGAGGGCTACGGCGTGTGGTGGGGACTGATCGCGGGCTTCGCAACCACTGCCGTGCTGCTATTGCGGACCTATTCTCGAC
GCCTCGCTTCAATTTCCTAA

Upstream 100 bases:

>100_bases
AGATCGCAGAGCGGCACGTGGAGAACATGCTGCGCGCCTACCGCGATTCGCGTTCCTTGTGCGCCGGCTGCCAGGCGCCA
CGATGCAGCGCGGTGCGGAG

Downstream 100 bases:

>100_bases
TGGGGCGTAAGACGGCTTTTACCTCCAAGCATTGCCTTTGATCGAAGGTGTGGCTAACCTAAGTAAGCCAACCCAAAGAA
AGGAACACAGTTGTCCCTCA

Product: multidrug and toxic compound extrusion family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRVAARLNSIQKYREVLGISLPIAGVQLAGVALTTTDVLMLQSVGVLAIAGGGLAMQLYNQIRTMCVGMVTASSNLVAEA
AAEMEREEASSQPSEEGAERIRRAVRSSFAVGTGTAVLGALLVITFGALVLALPIDEQVAHLTFAMTLTLAPGLIPMLWL
NVLRQFAVGMRKPGSLLAITLVSIFVNIALNWSFMAWATSDTWKVAGIGLSTTTVQLFTLLAFRVVVLRIPELQPYFQVL
PRAGDGETIRHLVRLGVPVSLTYGSEAAITTIAGLAMGTLSPQMLAAHNVVNQLAYIVYQVCIGFSHGGSVLITRARAEG
ANAVRAVTRRVLLVVVVYLGIMGALWLLLGRWILRPFLGSADATTFHIAVLLLCLAVLQQFSKGIQNVTVGLLRGLKDTT
SGLRCTLIGYWLVGVPGILVMGKLLRWEGYGVWWGLIAGFATTAVLLLRTYSRRLASIS

Sequences:

>Translated_459_residues
MRVAARLNSIQKYREVLGISLPIAGVQLAGVALTTTDVLMLQSVGVLAIAGGGLAMQLYNQIRTMCVGMVTASSNLVAEA
AAEMEREEASSQPSEEGAERIRRAVRSSFAVGTGTAVLGALLVITFGALVLALPIDEQVAHLTFAMTLTLAPGLIPMLWL
NVLRQFAVGMRKPGSLLAITLVSIFVNIALNWSFMAWATSDTWKVAGIGLSTTTVQLFTLLAFRVVVLRIPELQPYFQVL
PRAGDGETIRHLVRLGVPVSLTYGSEAAITTIAGLAMGTLSPQMLAAHNVVNQLAYIVYQVCIGFSHGGSVLITRARAEG
ANAVRAVTRRVLLVVVVYLGIMGALWLLLGRWILRPFLGSADATTFHIAVLLLCLAVLQQFSKGIQNVTVGLLRGLKDTT
SGLRCTLIGYWLVGVPGILVMGKLLRWEGYGVWWGLIAGFATTAVLLLRTYSRRLASIS
>Mature_459_residues
MRVAARLNSIQKYREVLGISLPIAGVQLAGVALTTTDVLMLQSVGVLAIAGGGLAMQLYNQIRTMCVGMVTASSNLVAEA
AAEMEREEASSQPSEEGAERIRRAVRSSFAVGTGTAVLGALLVITFGALVLALPIDEQVAHLTFAMTLTLAPGLIPMLWL
NVLRQFAVGMRKPGSLLAITLVSIFVNIALNWSFMAWATSDTWKVAGIGLSTTTVQLFTLLAFRVVVLRIPELQPYFQVL
PRAGDGETIRHLVRLGVPVSLTYGSEAAITTIAGLAMGTLSPQMLAAHNVVNQLAYIVYQVCIGFSHGGSVLITRARAEG
ANAVRAVTRRVLLVVVVYLGIMGALWLLLGRWILRPFLGSADATTFHIAVLLLCLAVLQQFSKGIQNVTVGLLRGLKDTT
SGLRCTLIGYWLVGVPGILVMGKLLRWEGYGVWWGLIAGFATTAVLLLRTYSRRLASIS

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=452, Percent_Identity=21.4601769911504, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49154; Mature: 49154

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAARLNSIQKYREVLGISLPIAGVQLAGVALTTTDVLMLQSVGVLAIAGGGLAMQLYN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEHHHHHHHHHHCCCEEEEECCHHHHHHHH
QIRTMCVGMVTASSNLVAEAAAEMEREEASSQPSEEGAERIRRAVRSSFAVGTGTAVLGA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LLVITFGALVLALPIDEQVAHLTFAMTLTLAPGLIPMLWLNVLRQFAVGMRKPGSLLAIT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVSIFVNIALNWSFMAWATSDTWKVAGIGLSTTTVQLFTLLAFRVVVLRIPELQPYFQVL
HHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
PRAGDGETIRHLVRLGVPVSLTYGSEAAITTIAGLAMGTLSPQMLAAHNVVNQLAYIVYQ
CCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VCIGFSHGGSVLITRARAEGANAVRAVTRRVLLVVVVYLGIMGALWLLLGRWILRPFLGS
HHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADATTFHIAVLLLCLAVLQQFSKGIQNVTVGLLRGLKDTTSGLRCTLIGYWLVGVPGILV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCHHHHH
MGKLLRWEGYGVWWGLIAGFATTAVLLLRTYSRRLASIS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAARLNSIQKYREVLGISLPIAGVQLAGVALTTTDVLMLQSVGVLAIAGGGLAMQLYN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEHHHHHHHHHHCCCEEEEECCHHHHHHHH
QIRTMCVGMVTASSNLVAEAAAEMEREEASSQPSEEGAERIRRAVRSSFAVGTGTAVLGA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LLVITFGALVLALPIDEQVAHLTFAMTLTLAPGLIPMLWLNVLRQFAVGMRKPGSLLAIT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LVSIFVNIALNWSFMAWATSDTWKVAGIGLSTTTVQLFTLLAFRVVVLRIPELQPYFQVL
HHHHHHHHHHCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
PRAGDGETIRHLVRLGVPVSLTYGSEAAITTIAGLAMGTLSPQMLAAHNVVNQLAYIVYQ
CCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VCIGFSHGGSVLITRARAEGANAVRAVTRRVLLVVVVYLGIMGALWLLLGRWILRPFLGS
HHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADATTFHIAVLLLCLAVLQQFSKGIQNVTVGLLRGLKDTTSGLRCTLIGYWLVGVPGILV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHCHHHHH
MGKLLRWEGYGVWWGLIAGFATTAVLLLRTYSRRLASIS
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]