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The map label for this gene is trf2 [H]

Identifier: 68537032

GI number: 68537032

Start: 2260033

End: 2261613

Strand: Direct

Name: trf2 [H]

Synonym: jk1938

Alternate gene names: 68537032

Gene position: 2260033-2261613 (Clockwise)

Preceding gene: 68537031

Following gene: 68537033

Centisome position: 91.78

GC content: 64.2

Gene sequence:

>1581_bases
ATGAAGCAATCCCAACAGCAGCCTCGTCCTTTCCGCAGCTCCCCTATCGCCGGAGCTCGGGATGCGTACACGGGCGTGGA
CTTCAACCTCGGGTTCCACGTGCTGCGCTACGATCTGCAGCTGGACTACGACGTAGAGCCCAACCACCTGCGCGGTGTGG
CCCGCCTGGCGGTCGAGAACTACCGTCCGCTGAAGACCATGACGTTGGACCTGGCCAACAATCTCGCTGTCAACCGCGTG
GAGGTCGCCGGCCACGGAGCGGCTGCCAACAACGGAACGATGAAGATCCGCCGCTTCCGGCACAACGGCAACAAGCTATC
GATCACTTTCGCAGAAGAGATTGCCGAGGATCAGTCCTTTGACCTGACCATCAAGTACGGCGGCTACCCGCGCCCGCTGC
GCTCCAAGTGGGGCGAGGTTGGCTGGGAGGAAACCGATGACGGAGCCCTGGTGGCAGGCCAGCCCAATGGCGCCCCCAGC
TGGTTCCCCTGCGACGATACGCCGGACGAAAAGGCAATGTTCCGCGTTGCCATCACCGCCCACAGAGACGTCACTGCCAT
CGCCCACGGCACCCTGGTGGAGCAGTACAGCAAGGGCAACAACACCACCCGGGTGTTTGTAACCAATTACCCGATGGCCA
CCTACCTGGCGGCGGTGTACGTTGGCCCCTTCAGGCGCGTCGACTTCCGCAGCGCGGAACTCGGCCGCAAGACCGTCCCG
GTATTCGGCTGGCTCCCCGAGGGCACCGAACACGCCAAGCGGGTCCGCGAGCTGGTCAACGAGGACTTCGGCCAGCAGAC
GGAAATGGTCGAGGTCTACTCGGAGCTCTTCGGCGCCTACCCCTTCCCGGAGTACTCCGTGGTGATCACGGACGAGGAGA
TGGAGATCCCCCTCGAAGCCCAGGGCATGAGCATGTTCGGCTGCAACCACGCCGACGGCAAGCACACCTGGGAGCGCCTG
ATCGCCCACGAGCTATCCCACCAGTGGTTCGGTAATTCCGTCGGCCTGGTCGAGTGGCGGGATATTTGGCTCAACGAAGG
CTTCGCCTGTTACTCCGAGTGGCTGTGGTTTGAAAAGTCTCAGGGCATCCCCGCCGCCCACCATGCGTGGGCGCACCATC
AGGCGCTCGCGGAGAAGCCGCAGGACATCCTGCTGATCGACCCCGGCGCGGACGACATGTTCGACGACCGGTTGTACAAG
CGCGGCGCTGTGACTGTCCACGCCCTGCGCGTGCTGCTGGGAGACGAGGCGTTCTTCGACATGGTTAAGCGCTGGACCAC
ACAGCACCGCATGGGGTTGGTGGAAACCCGCGACCTGGAGAACCTGGCAAACACCGTCGCCGAGGAAAACGGCGTGGCCC
GAAGTGAAGTCCAGGGGCTGTTCGACCGCTGGTTGCGTCACCAAGAGCTACCCGACTTCCCGGGCGGTGAGGGCATCGAG
CTCAGCGAGGAGCAGCTACGCGATCTTCCCGCGGGCCCCGCTGCGGAAATGGTCGATGCTTCCGGGCCGGCGCGTGCCGT
GGATGCGCTGAAGCGCGTTGGGCAACGGCTCGGCGAGCTCGGTGAGAGGCTACAGGACTAG

Upstream 100 bases:

>100_bases
CAGCGCCAGATAACAACACAACCAGATAAAACAACACTTCCCCCGCCACAGACCACCTCCGTGGCGGGGGTTGTCCTATG
CAGATAGTAGTGTGCCTTAC

Downstream 100 bases:

>100_bases
CCCTCTCTGGGCCGTCGGACGGGTTAACAAATGCGCGCCATATCGCTCGCCACAGTCTTCGTGGCCATCGCCGGTTACCT
GGTGATTTGGGTTGCCAACA

Product: putative metallopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKQSQQQPRPFRSSPIAGARDAYTGVDFNLGFHVLRYDLQLDYDVEPNHLRGVARLAVENYRPLKTMTLDLANNLAVNRV
EVAGHGAAANNGTMKIRRFRHNGNKLSITFAEEIAEDQSFDLTIKYGGYPRPLRSKWGEVGWEETDDGALVAGQPNGAPS
WFPCDDTPDEKAMFRVAITAHRDVTAIAHGTLVEQYSKGNNTTRVFVTNYPMATYLAAVYVGPFRRVDFRSAELGRKTVP
VFGWLPEGTEHAKRVRELVNEDFGQQTEMVEVYSELFGAYPFPEYSVVITDEEMEIPLEAQGMSMFGCNHADGKHTWERL
IAHELSHQWFGNSVGLVEWRDIWLNEGFACYSEWLWFEKSQGIPAAHHAWAHHQALAEKPQDILLIDPGADDMFDDRLYK
RGAVTVHALRVLLGDEAFFDMVKRWTTQHRMGLVETRDLENLANTVAEENGVARSEVQGLFDRWLRHQELPDFPGGEGIE
LSEEQLRDLPAGPAAEMVDASGPARAVDALKRVGQRLGELGERLQD

Sequences:

>Translated_526_residues
MKQSQQQPRPFRSSPIAGARDAYTGVDFNLGFHVLRYDLQLDYDVEPNHLRGVARLAVENYRPLKTMTLDLANNLAVNRV
EVAGHGAAANNGTMKIRRFRHNGNKLSITFAEEIAEDQSFDLTIKYGGYPRPLRSKWGEVGWEETDDGALVAGQPNGAPS
WFPCDDTPDEKAMFRVAITAHRDVTAIAHGTLVEQYSKGNNTTRVFVTNYPMATYLAAVYVGPFRRVDFRSAELGRKTVP
VFGWLPEGTEHAKRVRELVNEDFGQQTEMVEVYSELFGAYPFPEYSVVITDEEMEIPLEAQGMSMFGCNHADGKHTWERL
IAHELSHQWFGNSVGLVEWRDIWLNEGFACYSEWLWFEKSQGIPAAHHAWAHHQALAEKPQDILLIDPGADDMFDDRLYK
RGAVTVHALRVLLGDEAFFDMVKRWTTQHRMGLVETRDLENLANTVAEENGVARSEVQGLFDRWLRHQELPDFPGGEGIE
LSEEQLRDLPAGPAAEMVDASGPARAVDALKRVGQRLGELGERLQD
>Mature_526_residues
MKQSQQQPRPFRSSPIAGARDAYTGVDFNLGFHVLRYDLQLDYDVEPNHLRGVARLAVENYRPLKTMTLDLANNLAVNRV
EVAGHGAAANNGTMKIRRFRHNGNKLSITFAEEIAEDQSFDLTIKYGGYPRPLRSKWGEVGWEETDDGALVAGQPNGAPS
WFPCDDTPDEKAMFRVAITAHRDVTAIAHGTLVEQYSKGNNTTRVFVTNYPMATYLAAVYVGPFRRVDFRSAELGRKTVP
VFGWLPEGTEHAKRVRELVNEDFGQQTEMVEVYSELFGAYPFPEYSVVITDEEMEIPLEAQGMSMFGCNHADGKHTWERL
IAHELSHQWFGNSVGLVEWRDIWLNEGFACYSEWLWFEKSQGIPAAHHAWAHHQALAEKPQDILLIDPGADDMFDDRLYK
RGAVTVHALRVLLGDEAFFDMVKRWTTQHRMGLVETRDLENLANTVAEENGVARSEVQGLFDRWLRHQELPDFPGGEGIE
LSEEQLRDLPAGPAAEMVDASGPARAVDALKRVGQRLGELGERLQD

Specific function: Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome), yielding free amino acids [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI61742775, Length=464, Percent_Identity=22.6293103448276, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI61742777, Length=464, Percent_Identity=22.6293103448276, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI158937236, Length=335, Percent_Identity=25.6716417910448, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94818891, Length=454, Percent_Identity=22.6872246696035, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94818901, Length=454, Percent_Identity=22.6872246696035, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40316915, Length=526, Percent_Identity=25.4752851711027, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI310123622, Length=293, Percent_Identity=26.2798634812287, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310133497, Length=293, Percent_Identity=25.938566552901, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI132814467, Length=342, Percent_Identity=23.3918128654971, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI7019561, Length=348, Percent_Identity=22.7011494252874, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194306629, Length=372, Percent_Identity=25.2688172043011, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI11641261, Length=372, Percent_Identity=25.2688172043011, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4505029, Length=455, Percent_Identity=20.6593406593407, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI194239713, Length=421, Percent_Identity=22.3277909738717, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193206926, Length=479, Percent_Identity=22.5469728601253, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193206928, Length=479, Percent_Identity=22.5469728601253, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17565628, Length=427, Percent_Identity=24.824355971897, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI133903840, Length=416, Percent_Identity=24.7596153846154, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990873, Length=225, Percent_Identity=31.5555555555556, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533278, Length=363, Percent_Identity=23.6914600550964, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533276, Length=363, Percent_Identity=23.6914600550964, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989071, Length=207, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989076, Length=207, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17544504, Length=387, Percent_Identity=22.4806201550388, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71984353, Length=497, Percent_Identity=22.1327967806841, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71984339, Length=498, Percent_Identity=21.8875502008032, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17569225, Length=309, Percent_Identity=24.2718446601942, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI9755335, Length=468, Percent_Identity=23.5042735042735, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321837, Length=467, Percent_Identity=23.7687366167024, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6322054, Length=434, Percent_Identity=21.4285714285714, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324283, Length=449, Percent_Identity=24.0534521158129, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24646516, Length=517, Percent_Identity=23.5976789168279, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646518, Length=498, Percent_Identity=23.2931726907631, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24646512, Length=443, Percent_Identity=24.8306997742664, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24646510, Length=443, Percent_Identity=24.8306997742664, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24646514, Length=457, Percent_Identity=22.7571115973742, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358341, Length=402, Percent_Identity=25.1243781094527, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24655252, Length=472, Percent_Identity=25.2118644067797, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24655257, Length=466, Percent_Identity=25.7510729613734, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24655274, Length=466, Percent_Identity=25.7510729613734, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655260, Length=466, Percent_Identity=25.7510729613734, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655265, Length=466, Percent_Identity=25.7510729613734, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655268, Length=466, Percent_Identity=25.7510729613734, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221379089, Length=308, Percent_Identity=25.974025974026, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24651016, Length=413, Percent_Identity=23.4866828087167, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24651025, Length=475, Percent_Identity=22.3157894736842, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651023, Length=475, Percent_Identity=22.3157894736842, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651021, Length=475, Percent_Identity=22.3157894736842, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550850, Length=502, Percent_Identity=22.7091633466135, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24650973, Length=502, Percent_Identity=22.7091633466135, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221458256, Length=498, Percent_Identity=25.1004016064257, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362224, Length=498, Percent_Identity=25.1004016064257, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585062, Length=299, Percent_Identity=22.0735785953177, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585064, Length=299, Percent_Identity=22.0735785953177, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585066, Length=299, Percent_Identity=22.0735785953177, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550350, Length=383, Percent_Identity=21.4099216710183, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI161078673, Length=355, Percent_Identity=25.9154929577465, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571901, Length=355, Percent_Identity=25.9154929577465, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001930
- InterPro:   IPR014782
- InterPro:   IPR015568 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: NA

Molecular weight: Translated: 59310; Mature: 59310

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00142 ZINC_PROTEASE ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSQQQPRPFRSSPIAGARDAYTGVDFNLGFHVLRYDLQLDYDVEPNHLRGVARLAVEN
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEEEEEECCCCCHHHHHHHHHHHHC
YRPLKTMTLDLANNLAVNRVEVAGHGAAANNGTMKIRRFRHNGNKLSITFAEEIAEDQSF
CCCHHHEEEEHHCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEHHHHCCCCCE
DLTIKYGGYPRPLRSKWGEVGWEETDDGALVAGQPNGAPSWFPCDDTPDEKAMFRVAITA
EEEEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHEEEEEEEC
HRDVTAIAHGTLVEQYSKGNNTTRVFVTNYPMATYLAAVYVGPFRRVDFRSAELGRKTVP
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
VFGWLPEGTEHAKRVRELVNEDFGQQTEMVEVYSELFGAYPFPEYSVVITDEEMEIPLEA
EEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEE
QGMSMFGCNHADGKHTWERLIAHELSHQWFGNSVGLVEWRDIWLNEGFACYSEWLWFEKS
CCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHEECC
QGIPAAHHAWAHHQALAEKPQDILLIDPGADDMFDDRLYKRGAVTVHALRVLLGDEAFFD
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
MVKRWTTQHRMGLVETRDLENLANTVAEENGVARSEVQGLFDRWLRHQELPDFPGGEGIE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSEEQLRDLPAGPAAEMVDASGPARAVDALKRVGQRLGELGERLQD
CCHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSQQQPRPFRSSPIAGARDAYTGVDFNLGFHVLRYDLQLDYDVEPNHLRGVARLAVEN
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEEEEEEEEECCCCCHHHHHHHHHHHHC
YRPLKTMTLDLANNLAVNRVEVAGHGAAANNGTMKIRRFRHNGNKLSITFAEEIAEDQSF
CCCHHHEEEEHHCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEHHHHCCCCCE
DLTIKYGGYPRPLRSKWGEVGWEETDDGALVAGQPNGAPSWFPCDDTPDEKAMFRVAITA
EEEEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHEEEEEEEC
HRDVTAIAHGTLVEQYSKGNNTTRVFVTNYPMATYLAAVYVGPFRRVDFRSAELGRKTVP
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
VFGWLPEGTEHAKRVRELVNEDFGQQTEMVEVYSELFGAYPFPEYSVVITDEEMEIPLEA
EEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEE
QGMSMFGCNHADGKHTWERLIAHELSHQWFGNSVGLVEWRDIWLNEGFACYSEWLWFEKS
CCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHEECC
QGIPAAHHAWAHHQALAEKPQDILLIDPGADDMFDDRLYKRGAVTVHALRVLLGDEAFFD
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHH
MVKRWTTQHRMGLVETRDLENLANTVAEENGVARSEVQGLFDRWLRHQELPDFPGGEGIE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
LSEEQLRDLPAGPAAEMVDASGPARAVDALKRVGQRLGELGERLQD
CCHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11121031 [H]