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The map label for this gene is aceA [H]

Identifier: 68537027

GI number: 68537027

Start: 2252611

End: 2253906

Strand: Direct

Name: aceA [H]

Synonym: jk1933

Alternate gene names: 68537027

Gene position: 2252611-2253906 (Clockwise)

Preceding gene: 68537016

Following gene: 68537028

Centisome position: 91.48

GC content: 62.73

Gene sequence:

>1296_bases
ATGTCGAACGTCGGCAAGGCACGTACCGCTGAAGAAATCCAGAAGGACTGGGACGAGAACCCCCGCTGGGCTCACGTCGA
GCGCGATTACACCGCAGAGCAGGTCGCTGAGCTGCAGGGCACCGTTGTTGAAGAGCACACCCTGGCACGCCGCGGCGCTG
AGATCCTGTGGGAAGCAGTTAACAAGGATGACGACTCCTACATCAACGCCCTGGGCGCTCTGACCGGTAACCAGGCTGTT
CAGCAGGTCCGCGCAGGCCTGAAGGCTGTTTACCTGTCCGGTTGGCAGGTTGCAGGTGACGCTAACCTGTCCGGCCACAC
CTACCCCGACCAGTCCCTGTACCCGGCGAACTCCGTTCCGTCCGTCGTTCAGCGCATCAACAACGCGCTGTCCCGCGCAG
ACGAGATCGCTCGCGTTGAGGGCGACGACTCCGTTGACAACTGGCTGGTTCCGATCGTTGCCGACGGTGAGGCTGGCTTC
GGCGGCGCACTGAACGTTTACGAGCTGCAGAAGGCCATGATCAAGGCCGGCGCTGCAGGTACCCACTGGGAGGACCAGCT
GGCCTCCGAGAAGAAGTGTGGCCACCTGGGCGGCAAGGTCCTGATCCCGACCCAGCAGCACATCCGTACTCTGAACTCCG
CACGTCTGGCAGCTGACGTTGCCAACACCCCGACCCTGGTTGTTGCTCGTACCGACGCTGAGGCCGCTACCCTGCTGACC
TCCGACGTTGACGACCGCGACAAGCCGTTCCTGACCGGCGGCCGCACCGCTGAGGGCTTCTACAACGTGCAGAACGGCAT
CGAGCCCTGCATCGCCCGTGCGAAGGCATACGCTCCGTACGCTGACCTGATCTGGATGGAGACCGGCACCCCGGACCTGG
AGCTGGCTAAGAAGTTCGCTGAGGCTGTTCGCTCCGAGTACCCGGACCAGCTGCTGGCTTACAACTGCTCCCCGTCCTTC
AACTGGTCTGCACACCTGGACGACGACACCATCGCCAAGTTCCAGAACGAGCTGGGCGCAATGGGCTTCAAGTTCCAGTT
CATCACCCTGGCTGGCTTCCACGCTCTGAACCACTCCATGTTCGAACTGGCTTACGGCTACGCCCGCAACCAGATGACCG
CTTTCGTGGAGCTGCAGAACCGCGAGTTCGCTTCCGCCAAGGAGCGCGGCTTCACCGCTGTGAAGCACCAGCGCGAGGTC
GGTGCTGGCTACTTCGACAAGATCGCCACCACCGTCGACCCGAACTCCTCCACCACCGCTCTGAAGGGCTCCACCGAGGA
GGGCCAGTTCCACTAA

Upstream 100 bases:

>100_bases
CGAGGTTCTGGAGCATGCAGATCGTGTCCCCCCGCCAGGAACCCCCGGGCAGGGACCAAGACATAACTCTCCCCTTAATT
AGTAAGGAAGTGACCCCTAC

Downstream 100 bases:

>100_bases
GCCGCTTGTTGCCAAGCGCTAGGAACTAGCTGCGCGGGCGGTACAGCCCGCACACTACTTAAGTCCGGTCAGCACAAAAG
TGCTGGCCGGACTCTTTCTC

Product: hypothetical protein

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSNVGKARTAEEIQKDWDENPRWAHVERDYTAEQVAELQGTVVEEHTLARRGAEILWEAVNKDDDSYINALGALTGNQAV
QQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVQRINNALSRADEIARVEGDDSVDNWLVPIVADGEAGF
GGALNVYELQKAMIKAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTLVVARTDAEAATLLT
SDVDDRDKPFLTGGRTAEGFYNVQNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFAEAVRSEYPDQLLAYNCSPSF
NWSAHLDDDTIAKFQNELGAMGFKFQFITLAGFHALNHSMFELAYGYARNQMTAFVELQNREFASAKERGFTAVKHQREV
GAGYFDKIATTVDPNSSTTALKGSTEEGQFH

Sequences:

>Translated_431_residues
MSNVGKARTAEEIQKDWDENPRWAHVERDYTAEQVAELQGTVVEEHTLARRGAEILWEAVNKDDDSYINALGALTGNQAV
QQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVQRINNALSRADEIARVEGDDSVDNWLVPIVADGEAGF
GGALNVYELQKAMIKAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTLVVARTDAEAATLLT
SDVDDRDKPFLTGGRTAEGFYNVQNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFAEAVRSEYPDQLLAYNCSPSF
NWSAHLDDDTIAKFQNELGAMGFKFQFITLAGFHALNHSMFELAYGYARNQMTAFVELQNREFASAKERGFTAVKHQREV
GAGYFDKIATTVDPNSSTTALKGSTEEGQFH
>Mature_430_residues
SNVGKARTAEEIQKDWDENPRWAHVERDYTAEQVAELQGTVVEEHTLARRGAEILWEAVNKDDDSYINALGALTGNQAVQ
QVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVPSVVQRINNALSRADEIARVEGDDSVDNWLVPIVADGEAGFG
GALNVYELQKAMIKAGAAGTHWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTLVVARTDAEAATLLTS
DVDDRDKPFLTGGRTAEGFYNVQNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFAEAVRSEYPDQLLAYNCSPSFN
WSAHLDDDTIAKFQNELGAMGFKFQFITLAGFHALNHSMFELAYGYARNQMTAFVELQNREFASAKERGFTAVKHQREVG
AGYFDKIATTVDPNSSTTALKGSTEEGQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=60, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1786525, Length=310, Percent_Identity=27.0967741935484, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982926, Length=416, Percent_Identity=60.3365384615385, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17561814, Length=416, Percent_Identity=60.3365384615385, Blast_Score=505, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6320908, Length=271, Percent_Identity=35.0553505535055, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6325263, Length=253, Percent_Identity=35.9683794466403, Blast_Score=140, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47152; Mature: 47021

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVGKARTAEEIQKDWDENPRWAHVERDYTAEQVAELQGTVVEEHTLARRGAEILWEAV
CCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NKDDDSYINALGALTGNQAVQQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVP
CCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHH
SVVQRINNALSRADEIARVEGDDSVDNWLVPIVADGEAGFGGALNVYELQKAMIKAGAAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
THWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTLVVARTDAEAATLLT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHC
SDVDDRDKPFLTGGRTAEGFYNVQNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFA
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHH
EAVRSEYPDQLLAYNCSPSFNWSAHLDDDTIAKFQNELGAMGFKFQFITLAGFHALNHSM
HHHHHHCCCHHEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH
FELAYGYARNQMTAFVELQNREFASAKERGFTAVKHQREVGAGYFDKIATTVDPNSSTTA
HHHHHHHHHCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHEEECCCCCCEE
LKGSTEEGQFH
EECCCCCCCCC
>Mature Secondary Structure 
SNVGKARTAEEIQKDWDENPRWAHVERDYTAEQVAELQGTVVEEHTLARRGAEILWEAV
CCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NKDDDSYINALGALTGNQAVQQVRAGLKAVYLSGWQVAGDANLSGHTYPDQSLYPANSVP
CCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCHH
SVVQRINNALSRADEIARVEGDDSVDNWLVPIVADGEAGFGGALNVYELQKAMIKAGAAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
THWEDQLASEKKCGHLGGKVLIPTQQHIRTLNSARLAADVANTPTLVVARTDAEAATLLT
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHC
SDVDDRDKPFLTGGRTAEGFYNVQNGIEPCIARAKAYAPYADLIWMETGTPDLELAKKFA
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHH
EAVRSEYPDQLLAYNCSPSFNWSAHLDDDTIAKFQNELGAMGFKFQFITLAGFHALNHSM
HHHHHHCCCHHEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHH
FELAYGYARNQMTAFVELQNREFASAKERGFTAVKHQREVGAGYFDKIATTVDPNSSTTA
HHHHHHHHHCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHEEECCCCCCEE
LKGSTEEGQFH
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8206824; 12948626 [H]