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The map label for this gene is insI1 [H]

Identifier: 68536667

GI number: 68536667

Start: 1854635

End: 1855459

Strand: Direct

Name: insI1 [H]

Synonym: jk1581

Alternate gene names: 68536667

Gene position: 1854635-1855459 (Clockwise)

Preceding gene: 68536664

Following gene: 68536670

Centisome position: 75.32

GC content: 54.42

Gene sequence:

>825_bases
ATGCCAGCACCGATGAAAACTCAAGCCGTTGATGGATTTCCTAGCGGCGTATTCGACAAACACGCCTTAGTAGGGCGTGG
TCGCAGGCTTACTGTTGAAGACCGTGTTGCTATTGAAGCGGGATGTCGTGTCGGTGATTCGGCACGAGCCATCGCGCAGA
AAATAAATAGGCACCACAGTGTCGTCGCTCGTGAAATCACCCGTAATGGTTGGAAGATCGTCGATGAGGACGGCACAGAG
CAGCTGCGGTATAACGCCCACAACGCTGCTGTTTCTACTGCTGGTCGGATGGTGCGGCCAAAACTACGCAAGCTCGATGA
AAGCCCAACGCGTCGGGGTGTAGTAGTCGATTGCCTTGCCCGCAGGTGGTCACCTGGGCGCATTAGTGCATGGCTTGAGC
ATGCTTTCAGCGATGATGAAAGCATGCGTATTTCCCACGAAGCGATCTACAGTGCCTTATACATTCAAGGCAAAGGCAGC
CTGCGAGCCGAGCTTGAAGAAGTCATGAAGACTAAAGACGTACTTATCCGCGGCGGCTCAACACGCAAGCGTCGTGCCCG
AAACGCTGGCGTGTTAACCGGTCGCCCTTGGATCAAAGGTGCTGAGATCACCCACCGCAGCCCGGAAGCTGATGACAGGG
CTATTCCCGGGCACTGGGAGGGCGACCTTGTTATTGGTAATGAAGTGCCCCGGGTTTTGTTCCTAGGCATTTGCCTTGAT
TTCTATTATTGCTTGCGGCGGGTGCTGCTCCCAAAATTCGGTTTCCACTTCGACTGGGGTTCGGTATCCCAAGCTTTGGT
GAAGCCTCGTCTCGTTCCACCATGA

Upstream 100 bases:

>100_bases
GCGGTATGCGAAGTTACGCCGGCAGCGACATCGTCGAACGCCTGATGAGGCGGTGGTGTCTTTGCCAGATACAGGCAAGA
AGCTGGGTAAAGATTCTGTT

Downstream 100 bases:

>100_bases
CACCCACTCGAACGTCGCGATTTCTACCTCGACAACGTCATTCCACCTGCGAGTATGGATCAGCTCGTTTTTGTAGGAGC
CGTTAACGTTTTCAGCCAGA

Product: transposase for IS1513f

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPAPMKTQAVDGFPSGVFDKHALVGRGRRLTVEDRVAIEAGCRVGDSARAIAQKINRHHSVVAREITRNGWKIVDEDGTE
QLRYNAHNAAVSTAGRMVRPKLRKLDESPTRRGVVVDCLARRWSPGRISAWLEHAFSDDESMRISHEAIYSALYIQGKGS
LRAELEEVMKTKDVLIRGGSTRKRRARNAGVLTGRPWIKGAEITHRSPEADDRAIPGHWEGDLVIGNEVPRVLFLGICLD
FYYCLRRVLLPKFGFHFDWGSVSQALVKPRLVPP

Sequences:

>Translated_274_residues
MPAPMKTQAVDGFPSGVFDKHALVGRGRRLTVEDRVAIEAGCRVGDSARAIAQKINRHHSVVAREITRNGWKIVDEDGTE
QLRYNAHNAAVSTAGRMVRPKLRKLDESPTRRGVVVDCLARRWSPGRISAWLEHAFSDDESMRISHEAIYSALYIQGKGS
LRAELEEVMKTKDVLIRGGSTRKRRARNAGVLTGRPWIKGAEITHRSPEADDRAIPGHWEGDLVIGNEVPRVLFLGICLD
FYYCLRRVLLPKFGFHFDWGSVSQALVKPRLVPP
>Mature_273_residues
PAPMKTQAVDGFPSGVFDKHALVGRGRRLTVEDRVAIEAGCRVGDSARAIAQKINRHHSVVAREITRNGWKIVDEDGTEQ
LRYNAHNAAVSTAGRMVRPKLRKLDESPTRRGVVVDCLARRWSPGRISAWLEHAFSDDESMRISHEAIYSALYIQGKGSL
RAELEEVMKTKDVLIRGGSTRKRRARNAGVLTGRPWIKGAEITHRSPEADDRAIPGHWEGDLVIGNEVPRVLFLGICLDF
YYCLRRVLLPKFGFHFDWGSVSQALVKPRLVPP

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=208, Percent_Identity=33.6538461538462, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790736, Length=208, Percent_Identity=32.6923076923077, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787671, Length=208, Percent_Identity=32.6923076923077, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 30692; Mature: 30560

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPMKTQAVDGFPSGVFDKHALVGRGRRLTVEDRVAIEAGCRVGDSARAIAQKINRHHS
CCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VVAREITRNGWKIVDEDGTEQLRYNAHNAAVSTAGRMVRPKLRKLDESPTRRGVVVDCLA
HHHHHHHCCCCEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RRWSPGRISAWLEHAFSDDESMRISHEAIYSALYIQGKGSLRAELEEVMKTKDVLIRGGS
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHEEECCC
TRKRRARNAGVLTGRPWIKGAEITHRSPEADDRAIPGHWEGDLVIGNEVPRVLFLGICLD
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FYYCLRRVLLPKFGFHFDWGSVSQALVKPRLVPP
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PAPMKTQAVDGFPSGVFDKHALVGRGRRLTVEDRVAIEAGCRVGDSARAIAQKINRHHS
CCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VVAREITRNGWKIVDEDGTEQLRYNAHNAAVSTAGRMVRPKLRKLDESPTRRGVVVDCLA
HHHHHHHCCCCEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RRWSPGRISAWLEHAFSDDESMRISHEAIYSALYIQGKGSLRAELEEVMKTKDVLIRGGS
HCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHEEECCC
TRKRRARNAGVLTGRPWIKGAEITHRSPEADDRAIPGHWEGDLVIGNEVPRVLFLGICLD
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FYYCLRRVLLPKFGFHFDWGSVSQALVKPRLVPP
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]