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The map label for this gene is pthB

Identifier: 68536582

GI number: 68536582

Start: 1762650

End: 1763372

Strand: Direct

Name: pthB

Synonym: jk1496

Alternate gene names: 68536582

Gene position: 1762650-1763372 (Clockwise)

Preceding gene: 68536581

Following gene: 68536583

Centisome position: 71.58

GC content: 58.92

Gene sequence:

>723_bases
GTGGCTGTGTCTTTCCTCCAATCTCTGTTTTCCGTTTTCCGCAAGAAGTCTTCGCCACAGGAGCCTGCAACTACAACTGC
AGGTGGCCCTGCAAAGCGAACCAAGCTCACCGTCGGGGAGCTGCAAGAGTTCGCCCCTGAATGGATTGTGATCGGCCTGG
GCAATCCCGGCGCCAAGTACGCGGACACCCGGCACAATATCGGCTATTGGCCGATTGACCGCCTAGTTGAGCGGTATGAG
GCCCAGTGGCTGCCGGTGGAGGGCCAGAAGGCTCATGCGGCGCTGATCACGGTGGAAGAAACCCCCGTGCTGCTGCTCCG
CTCCACGACCTACATGAATAACTCTGGTGAGGCTGTGGGGCCGCTGGCTTCCGCTTTGAGTCTGCCGGCGGAGCGGATCA
TTGTCTGCCACGACGAGCTGGATATCGCCGCTGGGCAGGTCCGCATCAAGGATAAAGGTGGCGAGGGCGGCCACAATGGG
CTGCGTTCAATGACGGCCGAACTGGGCACTCAACACTACGTGCGCGTCCGCATGGGCATCGGTCGCCCGCCTAAGGGAAC
GTCAGTGATTGATTTTGTTCTGAGCCCGTTCGAAGAGGCAGACATCGACGCAGAGAACGGGTGGATGGAAAACACTCTTC
GGGATTCGGTGGACTCAGTGACGCTCATCGTGAACAATGGCACAGACATTGCGCGAAACGATATCCACACACGCAAGCAC
TAA

Upstream 100 bases:

>100_bases
ATGTGTTGAAGCCCTTCCCCAAGTCAGAGGCAGCGGAAGTGGCGATCATGTGCGCCGACGCGGCCGACGAGGTTGAGCGC
ACCCTCGGTGTGGGAAACTA

Downstream 100 bases:

>100_bases
TAAATGCTGCGGTGCAGGTAGCGCGCAGTAACGTCAATACTCGCGGGTTTTCGAGCTACCTAGCGTTAGTATTGATGAAT
CAATGCACGCGATCTTGCCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH 2

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAVSFLQSLFSVFRKKSSPQEPATTTAGGPAKRTKLTVGELQEFAPEWIVIGLGNPGAKYADTRHNIGYWPIDRLVERYE
AQWLPVEGQKAHAALITVEETPVLLLRSTTYMNNSGEAVGPLASALSLPAERIIVCHDELDIAAGQVRIKDKGGEGGHNG
LRSMTAELGTQHYVRVRMGIGRPPKGTSVIDFVLSPFEEADIDAENGWMENTLRDSVDSVTLIVNNGTDIARNDIHTRKH

Sequences:

>Translated_240_residues
MAVSFLQSLFSVFRKKSSPQEPATTTAGGPAKRTKLTVGELQEFAPEWIVIGLGNPGAKYADTRHNIGYWPIDRLVERYE
AQWLPVEGQKAHAALITVEETPVLLLRSTTYMNNSGEAVGPLASALSLPAERIIVCHDELDIAAGQVRIKDKGGEGGHNG
LRSMTAELGTQHYVRVRMGIGRPPKGTSVIDFVLSPFEEADIDAENGWMENTLRDSVDSVTLIVNNGTDIARNDIHTRKH
>Mature_239_residues
AVSFLQSLFSVFRKKSSPQEPATTTAGGPAKRTKLTVGELQEFAPEWIVIGLGNPGAKYADTRHNIGYWPIDRLVERYEA
QWLPVEGQKAHAALITVEETPVLLLRSTTYMNNSGEAVGPLASALSLPAERIIVCHDELDIAAGQVRIKDKGGEGGHNGL
RSMTAELGTQHYVRVRMGIGRPPKGTSVIDFVLSPFEEADIDAENGWMENTLRDSVDSVTLIVNNGTDIARNDIHTRKH

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=157, Percent_Identity=35.6687898089172, Blast_Score=88, Evalue=5e-18,
Organism=Escherichia coli, GI1787455, Length=194, Percent_Identity=35.5670103092784, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321983, Length=148, Percent_Identity=35.1351351351351, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH2_CORJK (Q4JU38)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_251287.1
- ProteinModelPortal:   Q4JU38
- SMR:   Q4JU38
- STRING:   Q4JU38
- GeneID:   3432995
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk1496
- NMPDR:   fig|306537.3.peg.749
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   IARNDIH
- ProtClustDB:   PRK05426
- BioCyc:   CJEI306537:JK1496-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 26242; Mature: 26111

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSFLQSLFSVFRKKSSPQEPATTTAGGPAKRTKLTVGELQEFAPEWIVIGLGNPGAKY
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEHHHHHHCCCCEEEEECCCCCCCC
ADTRHNIGYWPIDRLVERYEAQWLPVEGQKAHAALITVEETPVLLLRSTTYMNNSGEAVG
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCHHH
PLASALSLPAERIIVCHDELDIAAGQVRIKDKGGEGGHNGLRSMTAELGTQHYVRVRMGI
HHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECC
GRPPKGTSVIDFVLSPFEEADIDAENGWMENTLRDSVDSVTLIVNNGTDIARNDIHTRKH
CCCCCCCHHHHHHHCCHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCC
>Mature Secondary Structure 
AVSFLQSLFSVFRKKSSPQEPATTTAGGPAKRTKLTVGELQEFAPEWIVIGLGNPGAKY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEEHHHHHHCCCCEEEEECCCCCCCC
ADTRHNIGYWPIDRLVERYEAQWLPVEGQKAHAALITVEETPVLLLRSTTYMNNSGEAVG
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCHHH
PLASALSLPAERIIVCHDELDIAAGQVRIKDKGGEGGHNGLRSMTAELGTQHYVRVRMGI
HHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECC
GRPPKGTSVIDFVLSPFEEADIDAENGWMENTLRDSVDSVTLIVNNGTDIARNDIHTRKH
CCCCCCCHHHHHHHCCHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA