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The map label for this gene is pthA [H]

Identifier: 68536581

GI number: 68536581

Start: 1762021

End: 1762650

Strand: Direct

Name: pthA [H]

Synonym: jk1495

Alternate gene names: 68536581

Gene position: 1762021-1762650 (Clockwise)

Preceding gene: 68536580

Following gene: 68536582

Centisome position: 71.55

GC content: 59.21

Gene sequence:

>630_bases
GTGGACAGTACAATTACCGTCAGTGAGTTAGAGAGAGGGCTGGAGACGGTGGCGTCACCAAACAAGAAACAACACACCAA
CAGCGACACGTGGCTGATCGTCGGCCTGGGCAACCCCGGCGATAAATACGCCAATACCAGGCACAACGTTGGACGAATGG
TGATCGGCGAACTGCTGGATCGACAGGTACCGGCAGCCAGCCTGAATACCCACAAGAAGACGAACACGGACATCGCCGAG
GTGAAGATCGCGGGGCGCAAGGTGGTATTGGCACAGCCGCGAACTTTCATGAATGTATCGGGTGGGCCAGTGCAGCAGCT
GGCGGCGTTTTTCAAGATTCCGGCGGAGAACATCATCGTCGCCTACGACGACCTCGAGGGTGATCCGGGAGCAGTGAAGC
TGCGACAGTCCGGTGGGGACAAGGGGCATAACGGTCTGAAGTCCATTACGAAGTCGCTGGGGACGAAAGACTACTGGCGC
TTGTCGTGTGGCATTGGTCGGCCGCCCGGACGCATGGATCCAGCGGCCTATGTGTTGAAGCCCTTCCCCAAGTCAGAGGC
AGCGGAAGTGGCGATCATGTGCGCCGACGCGGCCGACGAGGTTGAGCGCACCCTCGGTGTGGGAAACTAG

Upstream 100 bases:

>100_bases
CCTTCACACACTGGGCCACACCGTGTGCATTTTCGCACCGGGTGGCCCTTTTTGTTGCCCTTTTTATTGACGCCACCCCA
CGGCCGCGGGGGCTGCCCGG

Downstream 100 bases:

>100_bases
TGGCTGTGTCTTTCCTCCAATCTCTGTTTTCCGTTTTCCGCAAGAAGTCTTCGCCACAGGAGCCTGCAACTACAACTGCA
GGTGGCCCTGCAAAGCGAAC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH 1 [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDSTITVSELERGLETVASPNKKQHTNSDTWLIVGLGNPGDKYANTRHNVGRMVIGELLDRQVPAASLNTHKKTNTDIAE
VKIAGRKVVLAQPRTFMNVSGGPVQQLAAFFKIPAENIIVAYDDLEGDPGAVKLRQSGGDKGHNGLKSITKSLGTKDYWR
LSCGIGRPPGRMDPAAYVLKPFPKSEAAEVAIMCADAADEVERTLGVGN

Sequences:

>Translated_209_residues
MDSTITVSELERGLETVASPNKKQHTNSDTWLIVGLGNPGDKYANTRHNVGRMVIGELLDRQVPAASLNTHKKTNTDIAE
VKIAGRKVVLAQPRTFMNVSGGPVQQLAAFFKIPAENIIVAYDDLEGDPGAVKLRQSGGDKGHNGLKSITKSLGTKDYWR
LSCGIGRPPGRMDPAAYVLKPFPKSEAAEVAIMCADAADEVERTLGVGN
>Mature_209_residues
MDSTITVSELERGLETVASPNKKQHTNSDTWLIVGLGNPGDKYANTRHNVGRMVIGELLDRQVPAASLNTHKKTNTDIAE
VKIAGRKVVLAQPRTFMNVSGGPVQQLAAFFKIPAENIIVAYDDLEGDPGAVKLRQSGGDKGHNGLKSITKSLGTKDYWR
LSCGIGRPPGRMDPAAYVLKPFPKSEAAEVAIMCADAADEVERTLGVGN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=171, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1787455, Length=173, Percent_Identity=40.4624277456647, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321983, Length=156, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 22422; Mature: 22422

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTITVSELERGLETVASPNKKQHTNSDTWLIVGLGNPGDKYANTRHNVGRMVIGELLD
CCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHH
RQVPAASLNTHKKTNTDIAEVKIAGRKVVLAQPRTFMNVSGGPVQQLAAFFKIPAENIIV
CCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEECCCCCHHHHHHHHHCCCCCEEE
AYDDLEGDPGAVKLRQSGGDKGHNGLKSITKSLGTKDYWRLSCGIGRPPGRMDPAAYVLK
EEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHEEC
PFPKSEAAEVAIMCADAADEVERTLGVGN
CCCCCCCCEEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MDSTITVSELERGLETVASPNKKQHTNSDTWLIVGLGNPGDKYANTRHNVGRMVIGELLD
CCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHH
RQVPAASLNTHKKTNTDIAEVKIAGRKVVLAQPRTFMNVSGGPVQQLAAFFKIPAENIIV
CCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEECCCCCHHHHHHHHHCCCCCEEE
AYDDLEGDPGAVKLRQSGGDKGHNGLKSITKSLGTKDYWRLSCGIGRPPGRMDPAAYVLK
EEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHEEC
PFPKSEAAEVAIMCADAADEVERTLGVGN
CCCCCCCCEEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA