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The map label for this gene is ephD [H]

Identifier: 68536231

GI number: 68536231

Start: 1354995

End: 1355834

Strand: Direct

Name: ephD [H]

Synonym: jk1154

Alternate gene names: 68536231

Gene position: 1354995-1355834 (Clockwise)

Preceding gene: 68536230

Following gene: 68536234

Centisome position: 55.03

GC content: 64.29

Gene sequence:

>840_bases
ATGTTTGCAGTAGTCACCGGAGCAGCCTCAGGTATTGGCCGGGAGGTGGCTCTGCAACTAGCTGAACGCGGTTATGCGGT
GGCCATCAGCGACCGCGACGCGGAAGGCCTGGCCACTACTGCCAGCGACATCACCACCTGCGGAGGCACCGTAGCCGACC
AGCGCACCGTGGACGTCACCGACGTGGAGGCAGTGAAGGCCTGGGCGGACGACGTGGTCGCAACCCACGGTGCCCCGGAC
CAGGTGCACCACGTGGCCGGTATCGCCATCTGGGGTGACGTGCGCCAGATGCCCCACGAAAAGTGGCAGCGCGTGATCGA
TGTGAACCTGATGGGTTCCGTGCACATGGTGGAGGCCTTCGCGCCGAAGCTCATGGAGAGCCAGCCGGTACGTCGGCGCC
GTCGCGACCGCCGGAAGTTGGTGTTCGTCTCCTCCGCCGCGGGCATTATCGGCCTGCCGTGGCACGCGGCCTATTCTGCT
TCCAAAGGTGGCGTGCTGGGCATGTGCGAGGTGCTGCGCTTCGACCTAGCCCCCTACGGCGTGGATGTGCACGCCGTCGC
CCCGGGTGCCGTGGACACTCCCCTGGTTCGCACCATCGACATCCACGGCGTGGATCGCTCCAAGAAGCGCATCCAGAAGG
CAACGGCCCTGTTCCAGGGCCACGCCGTAACCCCCGCCAAGGCCGCTCAAAAAATTTTGAAGGGAGTGGATAAAGGCAAG
TACCTCATCAACACCAGCCCTGACATCCCTGTTGCCCGCTGGGCGCAGGTCAACGCCCCCTGGGCCTACAAGGGCGTAAT
GAAGTCGCTTAACGCTGGGTTCAGGTGGGCGTCAAAGTAA

Upstream 100 bases:

>100_bases
CGCCGAAAAGCAGTATTGGCCCCACCCTGCTCTGACAGAATTAGTCGAGAACGCCCTACTTGGGTTAGATATCGAAAACT
AACAAAGGAGCCATCTCGCC

Downstream 100 bases:

>100_bases
GAAGAGCAACGAGATCGAGGCGAGCAACACGAGCGCAATGAAAGCGAGGAACGCCTTGCCCTGCCAGTGGGCGTAGTTGA
AGTCCATGCCGACCCCGAAG

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MFAVVTGAASGIGREVALQLAERGYAVAISDRDAEGLATTASDITTCGGTVADQRTVDVTDVEAVKAWADDVVATHGAPD
QVHHVAGIAIWGDVRQMPHEKWQRVIDVNLMGSVHMVEAFAPKLMESQPVRRRRRDRRKLVFVSSAAGIIGLPWHAAYSA
SKGGVLGMCEVLRFDLAPYGVDVHAVAPGAVDTPLVRTIDIHGVDRSKKRIQKATALFQGHAVTPAKAAQKILKGVDKGK
YLINTSPDIPVARWAQVNAPWAYKGVMKSLNAGFRWASK

Sequences:

>Translated_279_residues
MFAVVTGAASGIGREVALQLAERGYAVAISDRDAEGLATTASDITTCGGTVADQRTVDVTDVEAVKAWADDVVATHGAPD
QVHHVAGIAIWGDVRQMPHEKWQRVIDVNLMGSVHMVEAFAPKLMESQPVRRRRRDRRKLVFVSSAAGIIGLPWHAAYSA
SKGGVLGMCEVLRFDLAPYGVDVHAVAPGAVDTPLVRTIDIHGVDRSKKRIQKATALFQGHAVTPAKAAQKILKGVDKGK
YLINTSPDIPVARWAQVNAPWAYKGVMKSLNAGFRWASK
>Mature_279_residues
MFAVVTGAASGIGREVALQLAERGYAVAISDRDAEGLATTASDITTCGGTVADQRTVDVTDVEAVKAWADDVVATHGAPD
QVHHVAGIAIWGDVRQMPHEKWQRVIDVNLMGSVHMVEAFAPKLMESQPVRRRRRDRRKLVFVSSAAGIIGLPWHAAYSA
SKGGVLGMCEVLRFDLAPYGVDVHAVAPGAVDTPLVRTIDIHGVDRSKKRIQKATALFQGHAVTPAKAAQKILKGVDKGK
YLINTSPDIPVARWAQVNAPWAYKGVMKSLNAGFRWASK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI223718074, Length=249, Percent_Identity=27.3092369477912, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI20149619, Length=272, Percent_Identity=26.1029411764706, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4503817, Length=253, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI4758504, Length=206, Percent_Identity=32.0388349514563, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=28.7937743190661, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI7706318, Length=269, Percent_Identity=26.0223048327138, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI7657478, Length=193, Percent_Identity=28.4974093264249, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI32483357, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI83715985, Length=188, Percent_Identity=31.3829787234043, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI157739938, Length=258, Percent_Identity=22.4806201550388, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI40807363, Length=193, Percent_Identity=30.0518134715026, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI126723750, Length=221, Percent_Identity=27.1493212669683, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI44680136, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI44680133, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI17738292, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI50726952, Length=197, Percent_Identity=28.4263959390863, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787335, Length=198, Percent_Identity=29.7979797979798, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789057, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787526, Length=197, Percent_Identity=31.9796954314721, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788459, Length=194, Percent_Identity=25.7731958762887, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786701, Length=198, Percent_Identity=23.7373737373737, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082100, Length=198, Percent_Identity=26.7676767676768, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25147288, Length=198, Percent_Identity=31.3131313131313, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=201, Percent_Identity=32.8358208955224, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=205, Percent_Identity=33.1707317073171, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=196, Percent_Identity=30.1020408163265, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507613, Length=202, Percent_Identity=30.6930693069307, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32563809, Length=202, Percent_Identity=30.6930693069307, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=198, Percent_Identity=29.2929292929293, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=199, Percent_Identity=27.6381909547739, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=218, Percent_Identity=27.9816513761468, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=201, Percent_Identity=27.363184079602, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17557780, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=193, Percent_Identity=27.979274611399, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322067, Length=264, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=28.2051282051282, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=219, Percent_Identity=26.9406392694064, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21357041, Length=194, Percent_Identity=31.4432989690722, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28571526, Length=199, Percent_Identity=31.1557788944724, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24639444, Length=198, Percent_Identity=29.7979797979798, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358495, Length=263, Percent_Identity=26.615969581749, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355319, Length=198, Percent_Identity=28.7878787878788, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24644339, Length=196, Percent_Identity=29.0816326530612, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI23397609, Length=199, Percent_Identity=31.1557788944724, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355631, Length=174, Percent_Identity=28.1609195402299, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737361, Length=200, Percent_Identity=30, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649181, Length=174, Percent_Identity=28.735632183908, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364892, Length=174, Percent_Identity=28.735632183908, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584752, Length=194, Percent_Identity=28.3505154639175, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18859837, Length=198, Percent_Identity=28.2828282828283, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21356301, Length=245, Percent_Identity=24.4897959183673, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 30004; Mature: 30004

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVVTGAASGIGREVALQLAERGYAVAISDRDAEGLATTASDITTCGGTVADQRTVDVT
CEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEECHHHHHHCCCCCCCCCEECCH
DVEAVKAWADDVVATHGAPDQVHHVAGIAIWGDVRQMPHEKWQRVIDVNLMGSVHMVEAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEHHHHHCCHHHHHHEEEHEECCCHHHHHHH
APKLMESQPVRRRRRDRRKLVFVSSAAGIIGLPWHAAYSASKGGVLGMCEVLRFDLAPYG
HHHHHCCCHHHHHHHHHHEEEEEECCCCEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCC
VDVHAVAPGAVDTPLVRTIDIHGVDRSKKRIQKATALFQGHAVTPAKAAQKILKGVDKGK
EEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
YLINTSPDIPVARWAQVNAPWAYKGVMKSLNAGFRWASK
EEEECCCCCCHHHHEECCCCHHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MFAVVTGAASGIGREVALQLAERGYAVAISDRDAEGLATTASDITTCGGTVADQRTVDVT
CEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEECHHHHHHCCCCCCCCCEECCH
DVEAVKAWADDVVATHGAPDQVHHVAGIAIWGDVRQMPHEKWQRVIDVNLMGSVHMVEAF
HHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEHHHHHCCHHHHHHEEEHEECCCHHHHHHH
APKLMESQPVRRRRRDRRKLVFVSSAAGIIGLPWHAAYSASKGGVLGMCEVLRFDLAPYG
HHHHHCCCHHHHHHHHHHEEEEEECCCCEEECCCHHHCCCCCCCHHHHHHHHHHCCCCCC
VDVHAVAPGAVDTPLVRTIDIHGVDRSKKRIQKATALFQGHAVTPAKAAQKILKGVDKGK
EEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
YLINTSPDIPVARWAQVNAPWAYKGVMKSLNAGFRWASK
EEEECCCCCCHHHHEECCCCHHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]