Click here to switch to the map view.

The map label for this gene is sigA [H]

Identifier: 68536164

GI number: 68536164

Start: 1279337

End: 1280971

Strand: Direct

Name: sigA [H]

Synonym: jk1087

Alternate gene names: 68536164

Gene position: 1279337-1280971 (Clockwise)

Preceding gene: 68536163

Following gene: 68536168

Centisome position: 51.95

GC content: 61.28

Gene sequence:

>1635_bases
GTGGCAGCAAACGACTCGACCACCAGCAGCGAAGACAGCACCGAGGGTGTTGTACGCAAGGTTGCAAAGAAGACGGCAGC
GAAGAAGACTGCCCGAAAGGTGGCGAAGAAGTCGACGGTTGCCAAGAAGACCACCGTCCGAAGCACTGCAGCGAAGGCTG
CTGAAACTGCTGCCGAGCCGGAAGAGCAGGCGTCCACCCCTGTGAAGAAGGCGGCGAAGAAGACCGCGCGCAAGGCGGCG
AAGAAAACTGCCAAAAAGGCAGCTAAGAAAACTGCGAAGAAGACCACACGCAAGGTCGCCAAGAAGACGGCAAAGAAGAC
CACTGCGAAGCGTGGCACCAAGGCAGCAAAGGCAGCCGAGGACGTCCTCGAGGAAGACGAAGCTGCAACCAGCACCGATG
CACTATCGGCTAAGCAGGATGCGCCGGATAATCCGACTGAGCGCGACGAGTTCGACGATGATGCGGACGCTCCGGATGAC
CTCGATGCACTCGAGGATATGGACGAGGATGACGATCTCGATGGGTTGGATGACCTCGATGACCTCGAAGACGATGACGA
CGACATTGAAGACGAAGACCTCGAGGACGATGAGGACGAGGACGACGACGCAGAGGACGAGGACGAGGAGACCGATAAAA
ACGACGGCAGCTTTGTCTGGGACGAAGATGAATCCGCTGCACTGCGCCAGGCGCGTAAGGATGCAGAGCTCACCGCATCC
GCAGACTCCGTACGCGCCTATCTAAAGCAGATCGGTAAGGTCGCCCTGCTGAATGCTGAGCAGGAGGTCTCGCTGGCCAA
GCGCATCGAGGCCGGCTTGTACGCCGCTTATCGCCTGCAGGAGATCAAGGAATCTGGCGAGAAGCTCTCCCCGATGCACC
GCCGCGACCTCCGCGAAATTGACCGCGACGGGCGCCGTGCGAAGAACCACCTGTTGGAGGCCAACCTCCGCCTCGTTGTG
TCGCTAGCCAAGCGCTACACCGGTCGCGGCATGGCGTTCCTGGATCTGATCCAGGAAGGTAACCTCGGGCTGATCCGCGC
AGTCGAAAAGTTCGATTATGTGAAGGGCTACAAGTTCTCCACCTACGCCACCTGGTGGATCCGCCAGGCTATCACCCGCG
CCATGGCGGACCAGGCCCGCACCATCCGCATCCCGGTGCACATGGTCGAGGTCATCAACAAGCTGGGCCGCATTCAGCGC
GAGCTGCTGCAGGATCTGGGCCGCGAGCCCACTCCGGAGGAGCTGGCGCGCGAGATGGACATCACCGTGGACAAGGTGCT
GGAGATCCAGCAGTACGCCCGCGAGCCGATTTCTTTGGATCAGACCATCGGCGACGAGGGCGACTCCCAGCTGGGCGACT
TCATCGAGGACTCGGAGGCCGTCGTGGCCGTCGACGCGGTCTCGTTCACCCTGCTGCAGGATCAGCTGCAGGATGTGCTG
CACACCCTGTCCGAGCGAGAGGCCGGTGTGGTGAAGCTGCGCTTCGGCCTCACCGACGGAATGCCGCGCACTTTAGACGA
GATCGGCCAGGTGTACGGGGTCACCCGCGAGCGCATTCGCCAGATCGAGTCGAAGACGATGTCCAAGCTGCGCCACCCGT
CGCGCTCTCAGGTGCTGCGAGACTACCTTGACTAG

Upstream 100 bases:

>100_bases
TGGGTAAACCGCAACCCGGTGCGCCCTAATTCCCCCAAGGAATGTGGCCCGATAAGGCGGCCATCCTAGCGCACCATGTA
ATACGAGTGAAAGGGCTTCC

Downstream 100 bases:

>100_bases
CCCAGAACCAGTTCCGGCCTAGTTCTGGTTGTTCTTCACACAGTCGGAGAACTCCGTGCCACGTAGGTCTTCGCAACCTT
CCATGCTCTTCATAGCCACC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MAANDSTTSSEDSTEGVVRKVAKKTAAKKTARKVAKKSTVAKKTTVRSTAAKAAETAAEPEEQASTPVKKAAKKTARKAA
KKTAKKAAKKTAKKTTRKVAKKTAKKTTAKRGTKAAKAAEDVLEEDEAATSTDALSAKQDAPDNPTERDEFDDDADAPDD
LDALEDMDEDDDLDGLDDLDDLEDDDDDIEDEDLEDDEDEDDDAEDEDEETDKNDGSFVWDEDESAALRQARKDAELTAS
ADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYAAYRLQEIKESGEKLSPMHRRDLREIDRDGRRAKNHLLEANLRLVV
SLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYVKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQR
ELLQDLGREPTPEELAREMDITVDKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQDVL
HTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD

Sequences:

>Translated_544_residues
MAANDSTTSSEDSTEGVVRKVAKKTAAKKTARKVAKKSTVAKKTTVRSTAAKAAETAAEPEEQASTPVKKAAKKTARKAA
KKTAKKAAKKTAKKTTRKVAKKTAKKTTAKRGTKAAKAAEDVLEEDEAATSTDALSAKQDAPDNPTERDEFDDDADAPDD
LDALEDMDEDDDLDGLDDLDDLEDDDDDIEDEDLEDDEDEDDDAEDEDEETDKNDGSFVWDEDESAALRQARKDAELTAS
ADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYAAYRLQEIKESGEKLSPMHRRDLREIDRDGRRAKNHLLEANLRLVV
SLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYVKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQR
ELLQDLGREPTPEELAREMDITVDKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQDVL
HTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD
>Mature_543_residues
AANDSTTSSEDSTEGVVRKVAKKTAAKKTARKVAKKSTVAKKTTVRSTAAKAAETAAEPEEQASTPVKKAAKKTARKAAK
KTAKKAAKKTAKKTTRKVAKKTAKKTTAKRGTKAAKAAEDVLEEDEAATSTDALSAKQDAPDNPTERDEFDDDADAPDDL
DALEDMDEDDDLDGLDDLDDLEDDDDDIEDEDLEDDEDEDDDAEDEDEETDKNDGSFVWDEDESAALRQARKDAELTASA
DSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYAAYRLQEIKESGEKLSPMHRRDLREIDRDGRRAKNHLLEANLRLVVS
LAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYVKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRE
LLQDLGREPTPEELAREMDITVDKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQDVLH
TLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=274, Percent_Identity=56.2043795620438, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1789098, Length=345, Percent_Identity=36.8115942028986, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=31.1688311688312, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 60610; Mature: 60479

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANDSTTSSEDSTEGVVRKVAKKTAAKKTARKVAKKSTVAKKTTVRSTAAKAAETAAEP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEQASTPVKKAAKKTARKAAKKTAKKAAKKTAKKTTRKVAKKTAKKTTAKRGTKAAKAAE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DVLEEDEAATSTDALSAKQDAPDNPTERDEFDDDADAPDDLDALEDMDEDDDLDGLDDLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
DLEDDDDDIEDEDLEDDEDEDDDAEDEDEETDKNDGSFVWDEDESAALRQARKDAELTAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCC
ADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYAAYRLQEIKESGEKLSPMHRRDLREI
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DRDGRRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYVKGYKFS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
TYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAREMD
HHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
ITVDKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQDVL
CHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
HTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLR
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYLD
HHCC
>Mature Secondary Structure 
AANDSTTSSEDSTEGVVRKVAKKTAAKKTARKVAKKSTVAKKTTVRSTAAKAAETAAEP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEQASTPVKKAAKKTARKAAKKTAKKAAKKTAKKTTRKVAKKTAKKTTAKRGTKAAKAAE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DVLEEDEAATSTDALSAKQDAPDNPTERDEFDDDADAPDDLDALEDMDEDDDLDGLDDLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCC
DLEDDDDDIEDEDLEDDEDEDDDAEDEDEETDKNDGSFVWDEDESAALRQARKDAELTAS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHCC
ADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYAAYRLQEIKESGEKLSPMHRRDLREI
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DRDGRRAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYVKGYKFS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCH
TYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAREMD
HHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
ITVDKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQDQLQDVL
CHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
HTLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKLRHPSRSQVLR
HHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DYLD
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]