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The map label for this gene is smpB

Identifier: 68535528

GI number: 68535528

Start: 554828

End: 555382

Strand: Direct

Name: smpB

Synonym: jk0458

Alternate gene names: 68535528

Gene position: 554828-555382 (Clockwise)

Preceding gene: 68535527

Following gene: 68535529

Centisome position: 22.53

GC content: 59.64

Gene sequence:

>555_bases
ATGGCAGCAAAAAAGAGCACTCCCACCGATTCGGGGAAATCGAAGGGGAAGAAGAACAAGGCCCAGAAGGGCGCAGGTCA
GAAGGGGGCTGGCCAGATCGTCGTTGCCACCAACCGTAAGGCGCGCCACGATTACCACATCATCGATACCTACGAGTGCG
GTGTGGTTCTGGTGGGCACGGAAGTTAAGTCTTTGCGTGAGGGCAAGGCTTCTCTTGTTGACGCCTACGCCACCATCGAC
GAAGGTGAAGTCTGGCTGCGCGGCCTTCACATCCCGGAGTACTCCATGGGGCACTGGACGAACCACAGTCCCCGCCGTAC
CCGCAAGCTGCTGCTGCACCGCCGGGAGATCGACTCGCTTATGGGCAAGGTTCGCGACGGAAACAACACTTTGGTTCCCT
TGCAGCTGTACTTCGTTGCCGGCAAGTTGAAGGTCGAGCTGGCGCTGGCGCGTGGTAAGCAGGAATACGACAAGCGCCAG
GACATCAAGCGCCGTACGGAAGAGCGAGAGATCACGCGCGAGATGGGCCGGCGGATTAAGGGGATCCAGGGCTAG

Upstream 100 bases:

>100_bases
ATGTGAAGAACTAGCTCCGCGCCCTCGCTAAGCCAGCGCAGCATTAGCGCTTTAAAGCATTTTCGCTCGGGGGAGCGCCT
GGTGGTAGTGTGTGGCTGTC

Downstream 100 bases:

>100_bases
GGTGCGGTAACGCATAGGGGACCTCTATTGAACGGGGTTTGTGTTTTGATACTTTCGGGCGTGTGAAAAATGACGACGTC
GAGCATTGGTCCCTTAAGCA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAAKKSTPTDSGKSKGKKNKAQKGAGQKGAGQIVVATNRKARHDYHIIDTYECGVVLVGTEVKSLREGKASLVDAYATID
EGEVWLRGLHIPEYSMGHWTNHSPRRTRKLLLHRREIDSLMGKVRDGNNTLVPLQLYFVAGKLKVELALARGKQEYDKRQ
DIKRRTEEREITREMGRRIKGIQG

Sequences:

>Translated_184_residues
MAAKKSTPTDSGKSKGKKNKAQKGAGQKGAGQIVVATNRKARHDYHIIDTYECGVVLVGTEVKSLREGKASLVDAYATID
EGEVWLRGLHIPEYSMGHWTNHSPRRTRKLLLHRREIDSLMGKVRDGNNTLVPLQLYFVAGKLKVELALARGKQEYDKRQ
DIKRRTEEREITREMGRRIKGIQG
>Mature_183_residues
AAKKSTPTDSGKSKGKKNKAQKGAGQKGAGQIVVATNRKARHDYHIIDTYECGVVLVGTEVKSLREGKASLVDAYATIDE
GEVWLRGLHIPEYSMGHWTNHSPRRTRKLLLHRREIDSLMGKVRDGNNTLVPLQLYFVAGKLKVELALARGKQEYDKRQD
IKRRTEEREITREMGRRIKGIQG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=148, Percent_Identity=42.5675675675676, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_CORJK (Q4JX42)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250233.1
- HSSP:   Q8RR57
- ProteinModelPortal:   Q4JX42
- SMR:   Q4JX42
- STRING:   Q4JX42
- GeneID:   3433483
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0458
- NMPDR:   fig|306537.3.peg.2006
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   CJEI306537:JK0458-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 20748; Mature: 20617

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKKSTPTDSGKSKGKKNKAQKGAGQKGAGQIVVATNRKARHDYHIIDTYECGVVLVGT
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCEEEECC
EVKSLREGKASLVDAYATIDEGEVWLRGLHIPEYSMGHWTNHSPRRTRKLLLHRREIDSL
CHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MGKVRDGNNTLVPLQLYFVAGKLKVELALARGKQEYDKRQDIKRRTEEREITREMGRRIK
HHHCCCCCCCEEEEEEEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIQG
CCCC
>Mature Secondary Structure 
AAKKSTPTDSGKSKGKKNKAQKGAGQKGAGQIVVATNRKARHDYHIIDTYECGVVLVGT
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCEEEECC
EVKSLREGKASLVDAYATIDEGEVWLRGLHIPEYSMGHWTNHSPRRTRKLLLHRREIDSL
CHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MGKVRDGNNTLVPLQLYFVAGKLKVELALARGKQEYDKRQDIKRRTEEREITREMGRRIK
HHHCCCCCCCEEEEEEEEEECEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIQG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA