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The map label for this gene is echA2 [H]

Identifier: 68535501

GI number: 68535501

Start: 516129

End: 516923

Strand: Direct

Name: echA2 [H]

Synonym: jk0431

Alternate gene names: 68535501

Gene position: 516129-516923 (Clockwise)

Preceding gene: 68535500

Following gene: 68535504

Centisome position: 20.96

GC content: 65.41

Gene sequence:

>795_bases
ATGAGCGCAGGGCACGTCATCACTCATCTAGAGACCGTCGAGGCAGGCAAAGCGAGCAATGCCGACGGTTTCGGCCATTC
CGCCGCCCCACAGGTCGGCGTGATCACCCTCAACCGCCCAGAGAAGCGCAATGCCCTCAATGCCGCCATCGCCACAGTCG
TCGCAGAGGCCGTCCGCGCCCACACTGCCTCCGGGGTGCGTGCCATTTTGATTCGTGGCGAGGGACCTGTTTTCTGCGCC
GGAGCAGACCTTTCCGGCGGTGTGTACGCCGATGAATTTTGGTCCGCCATGGAGGACATGCTCTCGGCCATCCAGACCAC
CCCGGTGCCGGTCATCGCGGATGTTCACGGTCCGGCGGTCGGCGCGGGCTGCCAAATCGCCTTGGCCTGCGACCTGCGCG
CTTTTGCCGACGAGGGTGCCTGCTGGGTGCCTGTAGCCGAACACGGTTTCGCGCTGGACCTTTGGACTCACGCCCGCGCC
CGCGAGTTGATGGGCGGCGCACTGGCTCGCAACGTGTTCTTGGCAGGCGCCCGGGTAGGTGCCGAACAAGCTCGGGCGAC
GGGCTTTTCTGTGGTTCTGGATGACGCCACCCCCAACGCCAGCGCACTAGCGCATCGGGTGGCGCGCCAGGCTCCGCTTT
CTATGGAGCATTCGAAACGCGTGTTCAACAGCCCAGACATGCACGCGGATGCGGAGCTGCAGCAGATGATGGTGGACATC
TGGGCCAGCGAGGACGTACAGGAGGCGCGCCGCGCCCGCGCCGAGAAACGCGCGCCTCAGTTCAGAGGCCGTTAA

Upstream 100 bases:

>100_bases
CCGCTGACTTTCCACGCCGGCCTAGAGGGCTAGCGCAACTCCGCGCGGGTTACCGCGAAACACCCGCGTATGGCACCGTC
CCTGCGGTACATTGCTAGCC

Downstream 100 bases:

>100_bases
GGGACCATTCGCCGCCTAGTCGCGGCCTTCCACCGACCAGGTGTGCACTGGCTGGCCGCTGGACTGGTGGGCGATATAAC
GCTGCATCATGGTGGCCAGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSAGHVITHLETVEAGKASNADGFGHSAAPQVGVITLNRPEKRNALNAAIATVVAEAVRAHTASGVRAILIRGEGPVFCA
GADLSGGVYADEFWSAMEDMLSAIQTTPVPVIADVHGPAVGAGCQIALACDLRAFADEGACWVPVAEHGFALDLWTHARA
RELMGGALARNVFLAGARVGAEQARATGFSVVLDDATPNASALAHRVARQAPLSMEHSKRVFNSPDMHADAELQQMMVDI
WASEDVQEARRARAEKRAPQFRGR

Sequences:

>Translated_264_residues
MSAGHVITHLETVEAGKASNADGFGHSAAPQVGVITLNRPEKRNALNAAIATVVAEAVRAHTASGVRAILIRGEGPVFCA
GADLSGGVYADEFWSAMEDMLSAIQTTPVPVIADVHGPAVGAGCQIALACDLRAFADEGACWVPVAEHGFALDLWTHARA
RELMGGALARNVFLAGARVGAEQARATGFSVVLDDATPNASALAHRVARQAPLSMEHSKRVFNSPDMHADAELQQMMVDI
WASEDVQEARRARAEKRAPQFRGR
>Mature_263_residues
SAGHVITHLETVEAGKASNADGFGHSAAPQVGVITLNRPEKRNALNAAIATVVAEAVRAHTASGVRAILIRGEGPVFCAG
ADLSGGVYADEFWSAMEDMLSAIQTTPVPVIADVHGPAVGAGCQIALACDLRAFADEGACWVPVAEHGFALDLWTHARAR
ELMGGALARNVFLAGARVGAEQARATGFSVVLDDATPNASALAHRVARQAPLSMEHSKRVFNSPDMHADAELQQMMVDIW
ASEDVQEARRARAEKRAPQFRGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=28.5123966942149, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI70995211, Length=181, Percent_Identity=28.1767955801105, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=24.7933884297521, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=31.3253012048193, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI221142681, Length=247, Percent_Identity=29.1497975708502, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=26.0162601626016, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=25.3012048192771, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=31.2, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=225, Percent_Identity=26.2222222222222, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=28.6290322580645, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=26.3779527559055, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=26.4, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27834; Mature: 27703

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGHVITHLETVEAGKASNADGFGHSAAPQVGVITLNRPEKRNALNAAIATVVAEAVRA
CCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
HTASGVRAILIRGEGPVFCAGADLSGGVYADEFWSAMEDMLSAIQTTPVPVIADVHGPAV
HHCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GAGCQIALACDLRAFADEGACWVPVAEHGFALDLWTHARARELMGGALARNVFLAGARVG
CCCCEEEEEECHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQARATGFSVVLDDATPNASALAHRVARQAPLSMEHSKRVFNSPDMHADAELQQMMVDI
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
WASEDVQEARRARAEKRAPQFRGR
HCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAGHVITHLETVEAGKASNADGFGHSAAPQVGVITLNRPEKRNALNAAIATVVAEAVRA
CCCCEEHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
HTASGVRAILIRGEGPVFCAGADLSGGVYADEFWSAMEDMLSAIQTTPVPVIADVHGPAV
HHCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
GAGCQIALACDLRAFADEGACWVPVAEHGFALDLWTHARARELMGGALARNVFLAGARVG
CCCCEEEEEECHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
AEQARATGFSVVLDDATPNASALAHRVARQAPLSMEHSKRVFNSPDMHADAELQQMMVDI
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
WASEDVQEARRARAEKRAPQFRGR
HCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]