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The map label for this gene is cysS2

Identifier: 68535433

GI number: 68535433

Start: 441241

End: 442455

Strand: Direct

Name: cysS2

Synonym: jk0368

Alternate gene names: 68535433

Gene position: 441241-442455 (Clockwise)

Preceding gene: 68535432

Following gene: 68535434

Centisome position: 17.92

GC content: 67.65

Gene sequence:

>1215_bases
ATGCATGCATGGCCCGACCCGTCCGTACCCGTAGTCGCCGGCACCCCGGTGCCGCTGAAGCTCTTCGACACCGCCGATCA
GCGCGTCAAAGAAGTAGACACCACCCCGGATGCGAACGGGGAGGTGGGGATGTACGTCTGCGGCATTACCCCGTACGACT
CCACCCACCTCGGCCACGCCGCGACCTACCTCACCTTCGACCTGGCGCAGCGCCAGCTGCTCGCCAACGGCCACAAGGTC
CACTACGCGCAGAACATCACCGACGTGGACGACCCGCTGTTCGAGCGCGCCGAGCGCGACGGCGTGGACTGGCGCGAACT
CGGCACCAGCCAGATCAACCTGTTCCGCAGCGACATGGAGATCCTGTCCGTGATCCCGCCGCGCGACTACATCGGCGCGA
TGGAGTCAGTCGACGAGGTGATCGCGATGGTGCAGCAGCTTCTCGACGCCGGCGCCGCCTACGAGCTCGACCAGGGCGAC
ATTTACGCCTCCATCGACGCCACCGAGCAGTTCGGCTACGAGTCCAACCTGGACCGCGCCACCATGGAGGAGTACTTCGC
GGAACGTGGCGGCGACCCGGATCGAGAGGGCAAGCGCGATCCGCTGGACGCGCTGATCTGGCGCGGCCACCGCGAGGGCG
AGCCCGCCTGGGACTCACCGTTCGGCCCGGGCCGTCCGGGCTGGCACGTCGAGTGCTCCGCGATTGCCACCAACCGCCTG
GGCAGCCACTTCGCCATCCAGGGCGGCGGGTCTGACCTGGCGTTTCCGCACCACGAGTTCTCCGCGGCCCACGCTGAGGC
CGCGCTAAAGGTCGAGCGCATGGCCGGGCACTACGTCCACGCCGGCATGATCGCCCTCGACGGTGTGAAGATGTCCAAGT
CCCTGGGCAACCTCGTGTTCGTGCACAAGCTCTCGGAGGCAGGCCACGACCCGTCGGCGATCCGCCTGGCTGTCTTTGCG
GGCCACTACCGCGAGGACCGGGACTTCTCCGACGCGATCCTGGCCGAGGCCGAGGAGCGCCTTACGCGTTGGCGCGAGCA
GCTCGCCGGCGAGGTTTCTGAGGCGGAGGCGACGGAAGTCGTCGATAAGCTTCGCGCGATTCTTGCGGACGATCTGAACA
CCCCGGAGGCACTTTCGCTTCTCGACGGCGCAGCCGGCGACTGCAACCAGATCATCGCCACCGCCCTCGACGGGCTGCTC
GGGGTGCGCATTTAG

Upstream 100 bases:

>100_bases
TCGTTCGCGTGGTTCGCCGCCTACCGCATTCCGCTGGGCCTTTTGGTCATGGCGCTGCTCGGCACTGGCGTGATGGCCGC
CTAGTTGTAGGGTTGTCCGC

Downstream 100 bases:

>100_bases
CGCGCCTCACGCACAATGGGCCCATGAGCGCAGCTGCGGACAACTCGATTCGGGCCCAGTTTCAGCCCGAGGTAGACGAA
TTCATCGACGACCTGAACAC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase 1; Mycothiol ligase 1; MSH ligase 1

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MHAWPDPSVPVVAGTPVPLKLFDTADQRVKEVDTTPDANGEVGMYVCGITPYDSTHLGHAATYLTFDLAQRQLLANGHKV
HYAQNITDVDDPLFERAERDGVDWRELGTSQINLFRSDMEILSVIPPRDYIGAMESVDEVIAMVQQLLDAGAAYELDQGD
IYASIDATEQFGYESNLDRATMEEYFAERGGDPDREGKRDPLDALIWRGHREGEPAWDSPFGPGRPGWHVECSAIATNRL
GSHFAIQGGGSDLAFPHHEFSAAHAEAALKVERMAGHYVHAGMIALDGVKMSKSLGNLVFVHKLSEAGHDPSAIRLAVFA
GHYREDRDFSDAILAEAEERLTRWREQLAGEVSEAEATEVVDKLRAILADDLNTPEALSLLDGAAGDCNQIIATALDGLL
GVRI

Sequences:

>Translated_404_residues
MHAWPDPSVPVVAGTPVPLKLFDTADQRVKEVDTTPDANGEVGMYVCGITPYDSTHLGHAATYLTFDLAQRQLLANGHKV
HYAQNITDVDDPLFERAERDGVDWRELGTSQINLFRSDMEILSVIPPRDYIGAMESVDEVIAMVQQLLDAGAAYELDQGD
IYASIDATEQFGYESNLDRATMEEYFAERGGDPDREGKRDPLDALIWRGHREGEPAWDSPFGPGRPGWHVECSAIATNRL
GSHFAIQGGGSDLAFPHHEFSAAHAEAALKVERMAGHYVHAGMIALDGVKMSKSLGNLVFVHKLSEAGHDPSAIRLAVFA
GHYREDRDFSDAILAEAEERLTRWREQLAGEVSEAEATEVVDKLRAILADDLNTPEALSLLDGAAGDCNQIIATALDGLL
GVRI
>Mature_404_residues
MHAWPDPSVPVVAGTPVPLKLFDTADQRVKEVDTTPDANGEVGMYVCGITPYDSTHLGHAATYLTFDLAQRQLLANGHKV
HYAQNITDVDDPLFERAERDGVDWRELGTSQINLFRSDMEILSVIPPRDYIGAMESVDEVIAMVQQLLDAGAAYELDQGD
IYASIDATEQFGYESNLDRATMEEYFAERGGDPDREGKRDPLDALIWRGHREGEPAWDSPFGPGRPGWHVECSAIATNRL
GSHFAIQGGGSDLAFPHHEFSAAHAEAALKVERMAGHYVHAGMIALDGVKMSKSLGNLVFVHKLSEAGHDPSAIRLAVFA
GHYREDRDFSDAILAEAEERLTRWREQLAGEVSEAEATEVVDKLRAILADDLNTPEALSLLDGAAGDCNQIIATALDGLL
GVRI

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=372, Percent_Identity=32.258064516129, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21269877, Length=232, Percent_Identity=34.9137931034483, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI10835051, Length=232, Percent_Identity=34.9137931034483, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI62240992, Length=232, Percent_Identity=34.9137931034483, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI303304987, Length=232, Percent_Identity=34.9137931034483, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1786737, Length=386, Percent_Identity=37.0466321243523, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17509795, Length=253, Percent_Identity=32.4110671936759, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17509797, Length=253, Percent_Identity=32.4110671936759, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324082, Length=351, Percent_Identity=29.6296296296296, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922218, Length=362, Percent_Identity=34.8066298342541, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19922364, Length=234, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC1_CORJK (Q4JXD7)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250138.1
- ProteinModelPortal:   Q4JXD7
- SMR:   Q4JXD7
- STRING:   Q4JXD7
- GeneID:   3433754
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0368
- NMPDR:   fig|306537.3.peg.1581
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   ASIDATE
- ProtClustDB:   PRK12418
- BioCyc:   CJEI306537:JK0368-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.3.1.13

Molecular weight: Translated: 44156; Mature: 44156

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: BINDING 62-62 BINDING 228-228 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAWPDPSVPVVAGTPVPLKLFDTADQRVKEVDTTPDANGEVGMYVCGITPYDSTHLGHA
CCCCCCCCCCEEECCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHH
ATYLTFDLAQRQLLANGHKVHYAQNITDVDDPLFERAERDGVDWRELGTSQINLFRSDME
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHH
ILSVIPPRDYIGAMESVDEVIAMVQQLLDAGAAYELDQGDIYASIDATEQFGYESNLDRA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHCCCCCCCCHH
TMEEYFAERGGDPDREGKRDPLDALIWRGHREGEPAWDSPFGPGRPGWHVECSAIATNRL
HHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCC
GSHFAIQGGGSDLAFPHHEFSAAHAEAALKVERMAGHYVHAGMIALDGVKMSKSLGNLVF
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCHHEECCCHHHHHHCCEEE
VHKLSEAGHDPSAIRLAVFAGHYREDRDFSDAILAEAEERLTRWREQLAGEVSEAEATEV
EEEHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VDKLRAILADDLNTPEALSLLDGAAGDCNQIIATALDGLLGVRI
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHAWPDPSVPVVAGTPVPLKLFDTADQRVKEVDTTPDANGEVGMYVCGITPYDSTHLGHA
CCCCCCCCCCEEECCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHH
ATYLTFDLAQRQLLANGHKVHYAQNITDVDDPLFERAERDGVDWRELGTSQINLFRSDME
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHH
ILSVIPPRDYIGAMESVDEVIAMVQQLLDAGAAYELDQGDIYASIDATEQFGYESNLDRA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHCCCCCCCCHH
TMEEYFAERGGDPDREGKRDPLDALIWRGHREGEPAWDSPFGPGRPGWHVECSAIATNRL
HHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHCCC
GSHFAIQGGGSDLAFPHHEFSAAHAEAALKVERMAGHYVHAGMIALDGVKMSKSLGNLVF
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCHHEECCCHHHHHHCCEEE
VHKLSEAGHDPSAIRLAVFAGHYREDRDFSDAILAEAEERLTRWREQLAGEVSEAEATEV
EEEHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VDKLRAILADDLNTPEALSLLDGAAGDCNQIIATALDGLLGVRI
HHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA