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The map label for this gene is purF [H]

Identifier: 68535423

GI number: 68535423

Start: 429847

End: 431334

Strand: Direct

Name: purF [H]

Synonym: jk0358

Alternate gene names: 68535423

Gene position: 429847-431334 (Clockwise)

Preceding gene: 68535422

Following gene: 68535424

Centisome position: 17.46

GC content: 63.1

Gene sequence:

>1488_bases
TTGCTGAGTCCATTCGACGACCGGGGCGAAACTGAGCCCCGGGAAGAATGTGGCGTATTCGGAGTATGGGCACCGGGGGA
GGACGTTTCTAAGCTGACCTACTACGGCCTCTACGCCTTGCAGCATCGTGGCCAGGAGGCCGCAGGTATTGCGGTGGGCG
ATGGCGACCAGATCGTTGTATTCAAGGATCTGGGGCTTGTCAGCCAGGTCTTCGACGAACAGTCGCTGGAGGCCCTGAAG
GGGCACATCGGCTTGGGACATAACCGCTACACCACCGCCGGTGGAAATAGCTGGGAAAATGCGCAGCCCATGTTCCGAAT
GGCCCCCGATGGCACGGACGTAGCGCTGGGGCACAATGGCAACCTGGTGGATCACCGGGAGCTGACCCAGGAGGCCGCCC
GTCTGGGACTGGTGGATCCAAAGGAAAACCCCTCCGATTCCCTGGTGATGTGTGCCCTGCTGGCTGCCGACACACGCGAA
GGTCACGGGGTAGAAGAAGCAGCGATGGAGCTGTTCCCCAAGGTCAAGGGTGCATTCTGTGTGCTGTTCACCGACGGTGA
CGCGCTGTATGCGGCGCGCGACCCGCAGGGCGTGCGGCCGCTGTCCATCGGCCAGCTGGCAGGTGGCGGCTGGGTTGTGG
CTTCTGAAACCGCGGCGCTGGACATCGTGGGTGCGCGTTTCGTCCGCGATGTGGAGCCGGGCGAGCTGGTGATCGTCGAC
GGCGATGGCGTGCGCTCCCGCCGCTTCGCCGAGACCGCCCACAAGGGCTGCGTGTTTGAGTACGTATACCTGGCGCGCCC
CGATTCCGTGATCCGCGGCCGCTCGGTCAATGGCGCGCGTCTGCAGATCGGCCGGAAACTGGCGGAGGAAGCCCCGGCGG
ACGGCGACCTGGTCATCCCCGTTCCGGAATCCGGCACCCCGGCGGCGGTGGGATATGCCCAGGCTTCGCGCATCCCCTTC
GGCCAGGGGCTGATGAAGAACGCCTATGTGGGGCGCACCTTTATCCAGCCTTCGCAGACGATCCGTCAGCTGGGCATTCG
CTTGAAGCTCAACCCGCTGCGCGAGGTTATCGAGGGCAAGCGCCTGGTCGTTGTCGACGATTCGATCGTGCGTGGCAACA
CCCAGCGCGCTCTCATCAAGATGCTGCGTGAAGCGGGAGCGAAGGAAGTTCACGTCCGCATCGCTTCGCCGCCGGTGAAG
TGGCCGTGCTTCTACGGCATCGACTTTGCCTCGCCCACCGAGCTCATTGCGAATAACGTTGACGCCGCAGACCCGGTGGA
AGGGATCCGCAAGGAAATCGATGCGGATTCCCTAGCCTTCGTCTCGACCGAGGCGATGATAGAGGCGTCACAACAAAGCA
GCGACGAGCTGTGCGCGGCGTGCTTCGATGGCGTGTTCCCCCTAGGCATGCCGAGCGACAACCCGAATTCAGCGCTGGTC
GAAACAATGCTGAAGCAACAGGCTAAGAAAGCGAGTACTAAGCCATGA

Upstream 100 bases:

>100_bases
AAGATGAAACCCCATCCCTTTGGGTTAGCGCCCATGGGCTAGTAATGTCGGGATAGTGGCAAACATTCACGGCCAAGAAT
TTCAATCAGAGGCAGAAGCG

Downstream 100 bases:

>100_bases
GTGAGCAACCAGAGAACACCCCTGCACAGGCGGCAGGTGCGTCCTACGCCGCCGCAGGTGTGGATATTGAGGCCGGCGAT
CGTGCGGTAGAGCTCTTTGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MLSPFDDRGETEPREECGVFGVWAPGEDVSKLTYYGLYALQHRGQEAAGIAVGDGDQIVVFKDLGLVSQVFDEQSLEALK
GHIGLGHNRYTTAGGNSWENAQPMFRMAPDGTDVALGHNGNLVDHRELTQEAARLGLVDPKENPSDSLVMCALLAADTRE
GHGVEEAAMELFPKVKGAFCVLFTDGDALYAARDPQGVRPLSIGQLAGGGWVVASETAALDIVGARFVRDVEPGELVIVD
GDGVRSRRFAETAHKGCVFEYVYLARPDSVIRGRSVNGARLQIGRKLAEEAPADGDLVIPVPESGTPAAVGYAQASRIPF
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIEGKRLVVVDDSIVRGNTQRALIKMLREAGAKEVHVRIASPPVK
WPCFYGIDFASPTELIANNVDAADPVEGIRKEIDADSLAFVSTEAMIEASQQSSDELCAACFDGVFPLGMPSDNPNSALV
ETMLKQQAKKASTKP

Sequences:

>Translated_495_residues
MLSPFDDRGETEPREECGVFGVWAPGEDVSKLTYYGLYALQHRGQEAAGIAVGDGDQIVVFKDLGLVSQVFDEQSLEALK
GHIGLGHNRYTTAGGNSWENAQPMFRMAPDGTDVALGHNGNLVDHRELTQEAARLGLVDPKENPSDSLVMCALLAADTRE
GHGVEEAAMELFPKVKGAFCVLFTDGDALYAARDPQGVRPLSIGQLAGGGWVVASETAALDIVGARFVRDVEPGELVIVD
GDGVRSRRFAETAHKGCVFEYVYLARPDSVIRGRSVNGARLQIGRKLAEEAPADGDLVIPVPESGTPAAVGYAQASRIPF
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIEGKRLVVVDDSIVRGNTQRALIKMLREAGAKEVHVRIASPPVK
WPCFYGIDFASPTELIANNVDAADPVEGIRKEIDADSLAFVSTEAMIEASQQSSDELCAACFDGVFPLGMPSDNPNSALV
ETMLKQQAKKASTKP
>Mature_495_residues
MLSPFDDRGETEPREECGVFGVWAPGEDVSKLTYYGLYALQHRGQEAAGIAVGDGDQIVVFKDLGLVSQVFDEQSLEALK
GHIGLGHNRYTTAGGNSWENAQPMFRMAPDGTDVALGHNGNLVDHRELTQEAARLGLVDPKENPSDSLVMCALLAADTRE
GHGVEEAAMELFPKVKGAFCVLFTDGDALYAARDPQGVRPLSIGQLAGGGWVVASETAALDIVGARFVRDVEPGELVIVD
GDGVRSRRFAETAHKGCVFEYVYLARPDSVIRGRSVNGARLQIGRKLAEEAPADGDLVIPVPESGTPAAVGYAQASRIPF
GQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIEGKRLVVVDDSIVRGNTQRALIKMLREAGAKEVHVRIASPPVK
WPCFYGIDFASPTELIANNVDAADPVEGIRKEIDADSLAFVSTEAMIEASQQSSDELCAACFDGVFPLGMPSDNPNSALV
ETMLKQQAKKASTKP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=473, Percent_Identity=41.4376321353066, Blast_Score=335, Evalue=6e-92,
Organism=Escherichia coli, GI1788651, Length=477, Percent_Identity=38.3647798742138, Blast_Score=285, Evalue=6e-78,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=483, Percent_Identity=37.4741200828157, Blast_Score=291, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6323958, Length=479, Percent_Identity=34.2379958246347, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=41.4486921529175, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=41.7165668662675, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24659604, Length=472, Percent_Identity=41.9491525423729, Blast_Score=348, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53198; Mature: 53198

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPFDDRGETEPREECGVFGVWAPGEDVSKLTYYGLYALQHRGQEAAGIAVGDGDQIVV
CCCCCCCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEE
FKDLGLVSQVFDEQSLEALKGHIGLGHNRYTTAGGNSWENAQPMFRMAPDGTDVALGHNG
EECCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEECCCCCEEEECCCC
NLVDHRELTQEAARLGLVDPKENPSDSLVMCALLAADTREGHGVEEAAMELFPKVKGAFC
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
VLFTDGDALYAARDPQGVRPLSIGQLAGGGWVVASETAALDIVGARFVRDVEPGELVIVD
EEEECCCEEEEECCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEC
GDGVRSRRFAETAHKGCVFEYVYLARPDSVIRGRSVNGARLQIGRKLAEEAPADGDLVIP
CCCCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VPESGTPAAVGYAQASRIPFGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIEGK
CCCCCCCCEEEHHHHCCCCCCCCHHHCCCCCCEECCHHHHHHHHCCEEEEHHHHHHHCCC
RLVVVDDSIVRGNTQRALIKMLREAGAKEVHVRIASPPVKWPCFYGIDFASPTELIANNV
EEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHCCC
DAADPVEGIRKEIDADSLAFVSTEAMIEASQQSSDELCAACFDGVFPLGMPSDNPNSALV
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
ETMLKQQAKKASTKP
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSPFDDRGETEPREECGVFGVWAPGEDVSKLTYYGLYALQHRGQEAAGIAVGDGDQIVV
CCCCCCCCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEE
FKDLGLVSQVFDEQSLEALKGHIGLGHNRYTTAGGNSWENAQPMFRMAPDGTDVALGHNG
EECCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCEEECCCCCEEEECCCC
NLVDHRELTQEAARLGLVDPKENPSDSLVMCALLAADTREGHGVEEAAMELFPKVKGAFC
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
VLFTDGDALYAARDPQGVRPLSIGQLAGGGWVVASETAALDIVGARFVRDVEPGELVIVD
EEEECCCEEEEECCCCCCCCCCCCEECCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEC
GDGVRSRRFAETAHKGCVFEYVYLARPDSVIRGRSVNGARLQIGRKLAEEAPADGDLVIP
CCCCCHHHHHHHHHCCCEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
VPESGTPAAVGYAQASRIPFGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLREVIEGK
CCCCCCCCEEEHHHHCCCCCCCCHHHCCCCCCEECCHHHHHHHHCCEEEEHHHHHHHCCC
RLVVVDDSIVRGNTQRALIKMLREAGAKEVHVRIASPPVKWPCFYGIDFASPTELIANNV
EEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHCCC
DAADPVEGIRKEIDADSLAFVSTEAMIEASQQSSDELCAACFDGVFPLGMPSDNPNSALV
CCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
ETMLKQQAKKASTKP
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]