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The map label for this gene is purR [H]

Identifier: 68535394

GI number: 68535394

Start: 398624

End: 399733

Strand: Direct

Name: purR [H]

Synonym: jk0329

Alternate gene names: 68535394

Gene position: 398624-399733 (Clockwise)

Preceding gene: 68535389

Following gene: 68535400

Centisome position: 16.19

GC content: 65.86

Gene sequence:

>1110_bases
ATGAATAGACCAGCACGGCGCGGCACATTGGCCTCCATCGCGGCCGAACTGGGCGTTTCGCGCACCACAGTTTCGAACGC
ATACAACCGCCCGGATCAGCTTTCGCCCGAGTTGCGGGACAAGATCCTGCACGTCGCGGCGAAACTTGGCTACCCCGGGC
CAGACCCGATGGCGCGTTCGCTGCGCACGCGCCGAGCGGGGGCGATCGGCGTGCTGCTGACCGAGGAGCTGCCTTACGCG
TTCGAAGATCAGGCGTCGCTGGAGTTCGTCTCTGGCGTGTCAGTGAGCTGTGGGGCGATGGATGCCTCCATGCTGTTGAT
CCCCGCGGGCGTGGACGATAACCCCAGCGCCGATCGCCCCGCACAGCTGGTGAACCAGGCCAGCGTCGACGGCTTTATCG
TCTACTCGGTGGCGGGCGACGACCCGTACCTCGCCGCCGTGCGTAACCGCGGATTGCCGACCGTGGTGTGTGACCAACCC
GCCGGCGCCGGCGATCCTTTCGTGGGTATTGACGACCGCGAGGCCATCAAGCCCGCCATCCGTACCCTCGTCGACGCAGG
TCACACGCGCATCGGCGTGCTGTGCATCCGCCTGGATCGCACGCCGAACAACGGGCCTGTGTCCGCCGAACGCATCGAGG
GCGCGCACATGCACGTGCAGCGCTCGCGCCTGCTGGGAATCCTGGACGTGCTGGAGGAAGCCGGGATCGAGCAAGCCCCC
ATCGTCGAACGGCACATCAACAACCCCCAGACCGCCTTCGACGCCGCCCAGGAGTTACTTTCCACCCACCCGGAGCTCAC
CGCCGTTGCCTGCACCACCGACTCGCAGGCGCTCGGTGTGGTGCGTTACGCAGCGGAGCAGGGAATCGACGTGCCAGGTC
AGCTCTCTGTGACGGGGTTCGACGGTATTCCCGAAGCTATTGACGCCCACATCACCACCGTCCGCCAACCCAGCAAAGAC
AAGGGACTGGAGAGCGGCAACATGCTCGCTGAACTCATCGAGCAGCGGTTGAATGACAACCCGGCCAAGCCCGCGCACCG
GGCGGAGACGCCCACCGTGCTGCTGGAAACTGAGCTAATACGCGGAACGTCCGTCGCGCCGCCGCAGTAG

Upstream 100 bases:

>100_bases
CGTCAACATTAATCTTGAGCCAGATACGGGGATCTGGGCAGTGCCGGGGGAGGGGGCGTCAACCCCCAAGAAAGAAGGGC
TGAGGGCTACCCTTAGAAGA

Downstream 100 bases:

>100_bases
ATCCTGCAGGAAGTCCCGGACATCCTCCGGGGTATCAAAACGGTGGGTAGCGGAGGTCTCCCCGCCGCCGACGCGGATGC
CGATGTCTGGGGTGTTCGCA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNRPARRGTLASIAAELGVSRTTVSNAYNRPDQLSPELRDKILHVAAKLGYPGPDPMARSLRTRRAGAIGVLLTEELPYA
FEDQASLEFVSGVSVSCGAMDASMLLIPAGVDDNPSADRPAQLVNQASVDGFIVYSVAGDDPYLAAVRNRGLPTVVCDQP
AGAGDPFVGIDDREAIKPAIRTLVDAGHTRIGVLCIRLDRTPNNGPVSAERIEGAHMHVQRSRLLGILDVLEEAGIEQAP
IVERHINNPQTAFDAAQELLSTHPELTAVACTTDSQALGVVRYAAEQGIDVPGQLSVTGFDGIPEAIDAHITTVRQPSKD
KGLESGNMLAELIEQRLNDNPAKPAHRAETPTVLLETELIRGTSVAPPQ

Sequences:

>Translated_369_residues
MNRPARRGTLASIAAELGVSRTTVSNAYNRPDQLSPELRDKILHVAAKLGYPGPDPMARSLRTRRAGAIGVLLTEELPYA
FEDQASLEFVSGVSVSCGAMDASMLLIPAGVDDNPSADRPAQLVNQASVDGFIVYSVAGDDPYLAAVRNRGLPTVVCDQP
AGAGDPFVGIDDREAIKPAIRTLVDAGHTRIGVLCIRLDRTPNNGPVSAERIEGAHMHVQRSRLLGILDVLEEAGIEQAP
IVERHINNPQTAFDAAQELLSTHPELTAVACTTDSQALGVVRYAAEQGIDVPGQLSVTGFDGIPEAIDAHITTVRQPSKD
KGLESGNMLAELIEQRLNDNPAKPAHRAETPTVLLETELIRGTSVAPPQ
>Mature_369_residues
MNRPARRGTLASIAAELGVSRTTVSNAYNRPDQLSPELRDKILHVAAKLGYPGPDPMARSLRTRRAGAIGVLLTEELPYA
FEDQASLEFVSGVSVSCGAMDASMLLIPAGVDDNPSADRPAQLVNQASVDGFIVYSVAGDDPYLAAVRNRGLPTVVCDQP
AGAGDPFVGIDDREAIKPAIRTLVDAGHTRIGVLCIRLDRTPNNGPVSAERIEGAHMHVQRSRLLGILDVLEEAGIEQAP
IVERHINNPQTAFDAAQELLSTHPELTAVACTTDSQALGVVRYAAEQGIDVPGQLSVTGFDGIPEAIDAHITTVRQPSKD
KGLESGNMLAELIEQRLNDNPAKPAHRAETPTVLLETELIRGTSVAPPQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=29.7297297297297, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790369, Length=371, Percent_Identity=27.7628032345013, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790194, Length=354, Percent_Identity=28.2485875706215, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787580, Length=336, Percent_Identity=25.297619047619, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789202, Length=313, Percent_Identity=27.7955271565495, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=25.5451713395639, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=24.6710526315789, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=22.0543806646526, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39251; Mature: 39251

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPARRGTLASIAAELGVSRTTVSNAYNRPDQLSPELRDKILHVAAKLGYPGPDPMARS
CCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
LRTRRAGAIGVLLTEELPYAFEDQASLEFVSGVSVSCGAMDASMLLIPAGVDDNPSADRP
HHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCCCEECCCCCCEEEEEECCCCCCCCCCCH
AQLVNQASVDGFIVYSVAGDDPYLAAVRNRGLPTVVCDQPAGAGDPFVGIDDREAIKPAI
HHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHH
RTLVDAGHTRIGVLCIRLDRTPNNGPVSAERIEGAHMHVQRSRLLGILDVLEEAGIEQAP
HHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IVERHINNPQTAFDAAQELLSTHPELTAVACTTDSQALGVVRYAAEQGIDVPGQLSVTGF
HHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECC
DGIPEAIDAHITTVRQPSKDKGLESGNMLAELIEQRLNDNPAKPAHRAETPTVLLETELI
CCCHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHH
RGTSVAPPQ
CCCCCCCCC
>Mature Secondary Structure
MNRPARRGTLASIAAELGVSRTTVSNAYNRPDQLSPELRDKILHVAAKLGYPGPDPMARS
CCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHH
LRTRRAGAIGVLLTEELPYAFEDQASLEFVSGVSVSCGAMDASMLLIPAGVDDNPSADRP
HHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCCCEECCCCCCEEEEEECCCCCCCCCCCH
AQLVNQASVDGFIVYSVAGDDPYLAAVRNRGLPTVVCDQPAGAGDPFVGIDDREAIKPAI
HHHHHHCCCCEEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCCCEECCCCHHHHHHHH
RTLVDAGHTRIGVLCIRLDRTPNNGPVSAERIEGAHMHVQRSRLLGILDVLEEAGIEQAP
HHHHHCCCCEEEEEEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
IVERHINNPQTAFDAAQELLSTHPELTAVACTTDSQALGVVRYAAEQGIDVPGQLSVTGF
HHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEECC
DGIPEAIDAHITTVRQPSKDKGLESGNMLAELIEQRLNDNPAKPAHRAETPTVLLETELI
CCCHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHH
RGTSVAPPQ
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA