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The map label for this gene is cysS

Identifier: 68535378

GI number: 68535378

Start: 381341

End: 382777

Strand: Direct

Name: cysS

Synonym: jk0313

Alternate gene names: 68535378

Gene position: 381341-382777 (Clockwise)

Preceding gene: 68535376

Following gene: 68535380

Centisome position: 15.49

GC content: 64.93

Gene sequence:

>1437_bases
GTGACTTTGCGAATTTACGACACTGCCGCCCGCCAGCTCCGCGACTTTGAGCCCATCCGTGAAGGCCATGCCTCGATCTA
CCTGTGTGGAGCGACCGTGCAGTCGATCCCGCACATCGGGCATGTGCGCAGCGGAGTTGCTTTCGACATCCTGCGCAACT
GGCTAGAGGCCAAAGGGTTGGACGTCGCTTTCGTAAGGAACGTCACCGATATTGACGACAAGATCCTGACCAAGGCCGCG
GAGAACGACCGCCCGTGGTGGGAGTGGGCCGCTACGCACGAGCGTGCGTTTAACTGGGCTTATGACCAGTTGGGCGTCAC
CCCACCTTCCATCGAGCCCCGCGCCACAGGGCACGTACCGCAGATGATCGAGTACATGCAGCGCATCATCGACAACGGCC
ACGGCTACGCCGCAGACGGCAACGTGTACGCGCAACCCGCGACGATCGATAACTACGGCTTCCTGTCCGGCCAGAAGCTG
GACGAGATGGATCAGGGTGAGTCGGCGGGTACCGGCAAGCGCGACCCACGCGACTTCACCATGTGGAAGGCAGCCAAGCC
GGGCGAGCCAGCCTGGGATACCCCGTGGGGTCGTGGTCGCCCGGGCTGGCACATCGAGTGCTCCGCGATGGCGACGACCT
ACCTGGGAAGTGAGTTCGACATCCACTGCGGTGGCCTGGACCTGCAGTTCCCGCACCACGAGAACGAGGCCGCGCAGGCG
ACCGCAGCGGGCGATGGGTTCGCGCGCTACTGGATGCACAACGGCTGGGTGACGATGTCCGGCGAGAAGATGTCGAAGTC
CCTGGGCAACGTGCTTTCTATCCCGAATGTGCTGAAGCTGGTGCGTCCGGTTGAGCTGCGCTACTACCTGGGCAGCGCGC
ACTACCGCTCGATGCTGGAGTACTCCGAGGCTGCGCTAGGTGAGGCCGCTGCGGGTTACCGCCGCATCGAGAAGTTCCTG
GTGCGCGCTATAGAGTACGTCACCGGTGAGACCGCTGTGGACCCGCAGGCGCTGCCGGTCGGCGAGATGCCGGCGCAGTT
CGCGGAGAAGATGGATGACGACCTGGCCGTGCCGCAGTCGCTGGCCGTGATCCACGACGTGGTCCGCGAGGGCAACAAGC
TACTGGACGCAAAGCCCACGGACGAGGCAAAGCAGCAGGTTAAGGAGATCGCCGGACAGGTTCGTGCGATGGCTGCCGTG
CTGGGCGTGGATCCGCTGAGCGAGGCCTGGCTGGAATCTACCAAGGCTGCTGCCGGTGGTTCCGACGTGGCGATGGGCGC
CCTGGACGTGCTGGTGAAGGCCGAGCTGGAGCGCCGTGCTGAGGCCCGCGCAGCCAAGGACTGGGCCACCGCCGACGAGG
TGCGCGACCGCCTGGCCGAGGCCGGCATTGAGGTTACGGATACAGCCGATGGCGCGAAGTGGTCGCTGAAGGGGTAG

Upstream 100 bases:

>100_bases
GGATGGTGGCGAATCTCGGGGTGTTTTGAGTGCTCATGTCCTCCACGATACTGACACGCATGGGGAAAGAGGTCGACCGT
GCATGCCTATACTGTGTGGT

Downstream 100 bases:

>100_bases
TTCCATAGACGTACTCGTCGGGTTGTAACTCTGATCCCGGAAGCTCGACCTCGTCGTCATACATTTCTTCGGGTACGAAA
TATTCTTCGTCGTTCGACCA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTLRIYDTAARQLRDFEPIREGHASIYLCGATVQSIPHIGHVRSGVAFDILRNWLEAKGLDVAFVRNVTDIDDKILTKAA
ENDRPWWEWAATHERAFNWAYDQLGVTPPSIEPRATGHVPQMIEYMQRIIDNGHGYAADGNVYAQPATIDNYGFLSGQKL
DEMDQGESAGTGKRDPRDFTMWKAAKPGEPAWDTPWGRGRPGWHIECSAMATTYLGSEFDIHCGGLDLQFPHHENEAAQA
TAAGDGFARYWMHNGWVTMSGEKMSKSLGNVLSIPNVLKLVRPVELRYYLGSAHYRSMLEYSEAALGEAAAGYRRIEKFL
VRAIEYVTGETAVDPQALPVGEMPAQFAEKMDDDLAVPQSLAVIHDVVREGNKLLDAKPTDEAKQQVKEIAGQVRAMAAV
LGVDPLSEAWLESTKAAAGGSDVAMGALDVLVKAELERRAEARAAKDWATADEVRDRLAEAGIEVTDTADGAKWSLKG

Sequences:

>Translated_478_residues
MTLRIYDTAARQLRDFEPIREGHASIYLCGATVQSIPHIGHVRSGVAFDILRNWLEAKGLDVAFVRNVTDIDDKILTKAA
ENDRPWWEWAATHERAFNWAYDQLGVTPPSIEPRATGHVPQMIEYMQRIIDNGHGYAADGNVYAQPATIDNYGFLSGQKL
DEMDQGESAGTGKRDPRDFTMWKAAKPGEPAWDTPWGRGRPGWHIECSAMATTYLGSEFDIHCGGLDLQFPHHENEAAQA
TAAGDGFARYWMHNGWVTMSGEKMSKSLGNVLSIPNVLKLVRPVELRYYLGSAHYRSMLEYSEAALGEAAAGYRRIEKFL
VRAIEYVTGETAVDPQALPVGEMPAQFAEKMDDDLAVPQSLAVIHDVVREGNKLLDAKPTDEAKQQVKEIAGQVRAMAAV
LGVDPLSEAWLESTKAAAGGSDVAMGALDVLVKAELERRAEARAAKDWATADEVRDRLAEAGIEVTDTADGAKWSLKG
>Mature_477_residues
TLRIYDTAARQLRDFEPIREGHASIYLCGATVQSIPHIGHVRSGVAFDILRNWLEAKGLDVAFVRNVTDIDDKILTKAAE
NDRPWWEWAATHERAFNWAYDQLGVTPPSIEPRATGHVPQMIEYMQRIIDNGHGYAADGNVYAQPATIDNYGFLSGQKLD
EMDQGESAGTGKRDPRDFTMWKAAKPGEPAWDTPWGRGRPGWHIECSAMATTYLGSEFDIHCGGLDLQFPHHENEAAQAT
AAGDGFARYWMHNGWVTMSGEKMSKSLGNVLSIPNVLKLVRPVELRYYLGSAHYRSMLEYSEAALGEAAAGYRRIEKFLV
RAIEYVTGETAVDPQALPVGEMPAQFAEKMDDDLAVPQSLAVIHDVVREGNKLLDAKPTDEAKQQVKEIAGQVRAMAAVL
GVDPLSEAWLESTKAAAGGSDVAMGALDVLVKAELERRAEARAAKDWATADEVRDRLAEAGIEVTDTADGAKWSLKG

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=474, Percent_Identity=34.3881856540084, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI10835051, Length=335, Percent_Identity=35.5223880597015, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI62240992, Length=292, Percent_Identity=40.0684931506849, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI21269877, Length=335, Percent_Identity=35.5223880597015, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI303304987, Length=325, Percent_Identity=36, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1786737, Length=482, Percent_Identity=43.1535269709544, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17509797, Length=473, Percent_Identity=28.3298097251586, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17509795, Length=473, Percent_Identity=28.3298097251586, Blast_Score=181, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=401, Percent_Identity=33.4164588528678, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI19922218, Length=473, Percent_Identity=32.76955602537, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI19922364, Length=244, Percent_Identity=41.8032786885246, Blast_Score=188, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CORJK (Q4JXJ2)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_250083.1
- ProteinModelPortal:   Q4JXJ2
- SMR:   Q4JXJ2
- STRING:   Q4JXJ2
- GeneID:   3433755
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0313
- NMPDR:   fig|306537.3.peg.1127
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- ProtClustDB:   PRK00260
- BioCyc:   CJEI306537:JK0313-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52582; Mature: 52450

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRIYDTAARQLRDFEPIREGHASIYLCGATVQSIPHIGHVRSGVAFDILRNWLEAKGL
CEEEEEHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHCCHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKAAENDRPWWEWAATHERAFNWAYDQLGVTPPSIEPRATGHVP
CEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
QMIEYMQRIIDNGHGYAADGNVYAQPATIDNYGFLSGQKLDEMDQGESAGTGKRDPRDFT
HHHHHHHHHHHCCCCEEECCCEEECCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
MWKAAKPGEPAWDTPWGRGRPGWHIECSAMATTYLGSEFDIHCGGLDLQFPHHENEAAQA
EEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHH
TAAGDGFARYWMHNGWVTMSGEKMSKSLGNVLSIPNVLKLVRPVELRYYLGSAHYRSMLE
HHCCCCHHHHEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
YSEAALGEAAAGYRRIEKFLVRAIEYVTGETAVDPQALPVGEMPAQFAEKMDDDLAVPQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHCCHHH
LAVIHDVVREGNKLLDAKPTDEAKQQVKEIAGQVRAMAAVLGVDPLSEAWLESTKAAAGG
HHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SDVAMGALDVLVKAELERRAEARAAKDWATADEVRDRLAEAGIEVTDTADGAKWSLKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEECCCC
>Mature Secondary Structure 
TLRIYDTAARQLRDFEPIREGHASIYLCGATVQSIPHIGHVRSGVAFDILRNWLEAKGL
EEEEEHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCHHHHHCCHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKAAENDRPWWEWAATHERAFNWAYDQLGVTPPSIEPRATGHVP
CEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
QMIEYMQRIIDNGHGYAADGNVYAQPATIDNYGFLSGQKLDEMDQGESAGTGKRDPRDFT
HHHHHHHHHHHCCCCEEECCCEEECCCEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
MWKAAKPGEPAWDTPWGRGRPGWHIECSAMATTYLGSEFDIHCGGLDLQFPHHENEAAQA
EEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHH
TAAGDGFARYWMHNGWVTMSGEKMSKSLGNVLSIPNVLKLVRPVELRYYLGSAHYRSMLE
HHCCCCHHHHEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
YSEAALGEAAAGYRRIEKFLVRAIEYVTGETAVDPQALPVGEMPAQFAEKMDDDLAVPQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHCCHHH
LAVIHDVVREGNKLLDAKPTDEAKQQVKEIAGQVRAMAAVLGVDPLSEAWLESTKAAAGG
HHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
SDVAMGALDVLVKAELERRAEARAAKDWATADEVRDRLAEAGIEVTDTADGAKWSLKG
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA