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The map label for this gene is folK [H]

Identifier: 68535347

GI number: 68535347

Start: 345624

End: 346226

Strand: Direct

Name: folK [H]

Synonym: jk0282

Alternate gene names: 68535347

Gene position: 345624-346226 (Clockwise)

Preceding gene: 68535346

Following gene: 68535348

Centisome position: 14.04

GC content: 64.51

Gene sequence:

>603_bases
ATGATCACAGCCTTCCTGGGAATTGGCTCGAACATGCCCGGCGACGTGGGCAGCCCCACCGCACAGATCGAACGCGCCTA
CGAGCTGCTGGCGATGCACCCGGCCATCGAGATCGTGGAGAAGTCGCGGATCTTCGTCACCCCGCCGTGGGGTGGGGTGG
AGCAGCAGGAGTTCCGCAATTCCGTGGTGGCCGTACGCACGTCGCTGGATCCGCTCGCGCTGCTGCACCACTGCCAATTT
GCCGAGGCCGTCGCGGGGCGGGAGCGCGAGGTCCGGTGGGGCCCGCGCACCGTCGATGTGGACATTCTTTTTTGTTTTGA
CGCCCAGTCCCAGCCCCTGCAGTCCCAGGGAAAGTGGGGGTTTGAGCTGGTTATTCCGCACCCCTACGCCCACGAGCGGG
CGTTTGTGCTGGTGCCGCTGAGCGACCTGCAGCTGGCCCGGGAACCCTGGTGTACCTTGGGCGGGCGAGGCATCCTGGAG
TGGCTAGAAGAGGTTGATCCGGCCGAGCGGAAGGCTATCGTCCCGGCGGATCGGTCCGAACTATCGGTCCCTGGCGAGGG
CCAAAGCCTAGGAACAAATACTAGGACCAAAGACGAGGGATAA

Upstream 100 bases:

>100_bases
TGGGCGGGGTGTACGCCGTGGAGGTCACGGTGCATAAGCCACAGGCGCCGATCGCCCACCCGTTCGGCGACGTGCGGGTG
GTGGCTCGGAGGTCCAAGCG

Downstream 100 bases:

>100_bases
AGAGGCAGCACATGAGCCCGCTGAAGCGCACGAAGATCTCCACGCTGGCTGTCGTGGCGATCGTGTGCGCCCTCATCGCC
TGGGTCAGCACGCTGAGTTT

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MITAFLGIGSNMPGDVGSPTAQIERAYELLAMHPAIEIVEKSRIFVTPPWGGVEQQEFRNSVVAVRTSLDPLALLHHCQF
AEAVAGREREVRWGPRTVDVDILFCFDAQSQPLQSQGKWGFELVIPHPYAHERAFVLVPLSDLQLAREPWCTLGGRGILE
WLEEVDPAERKAIVPADRSELSVPGEGQSLGTNTRTKDEG

Sequences:

>Translated_200_residues
MITAFLGIGSNMPGDVGSPTAQIERAYELLAMHPAIEIVEKSRIFVTPPWGGVEQQEFRNSVVAVRTSLDPLALLHHCQF
AEAVAGREREVRWGPRTVDVDILFCFDAQSQPLQSQGKWGFELVIPHPYAHERAFVLVPLSDLQLAREPWCTLGGRGILE
WLEEVDPAERKAIVPADRSELSVPGEGQSLGTNTRTKDEG
>Mature_200_residues
MITAFLGIGSNMPGDVGSPTAQIERAYELLAMHPAIEIVEKSRIFVTPPWGGVEQQEFRNSVVAVRTSLDPLALLHHCQF
AEAVAGREREVRWGPRTVDVDILFCFDAQSQPLQSQGKWGFELVIPHPYAHERAFVLVPLSDLQLAREPWCTLGGRGILE
WLEEVDPAERKAIVPADRSELSVPGEGQSLGTNTRTKDEG

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=144, Percent_Identity=35.4166666666667, Blast_Score=81, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324073, Length=141, Percent_Identity=34.0425531914894, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 22123; Mature: 22123

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAFLGIGSNMPGDVGSPTAQIERAYELLAMHPAIEIVEKSRIFVTPPWGGVEQQEFRN
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHC
SVVAVRTSLDPLALLHHCQFAEAVAGREREVRWGPRTVDVDILFCFDAQSQPLQSQGKWG
CEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCCCCC
FELVIPHPYAHERAFVLVPLSDLQLAREPWCTLGGRGILEWLEEVDPAERKAIVPADRSE
EEEEECCCCCCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCC
LSVPGEGQSLGTNTRTKDEG
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITAFLGIGSNMPGDVGSPTAQIERAYELLAMHPAIEIVEKSRIFVTPPWGGVEQQEFRN
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHC
SVVAVRTSLDPLALLHHCQFAEAVAGREREVRWGPRTVDVDILFCFDAQSQPLQSQGKWG
CEEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEEEEEECCCCCCCCCCCCCC
FELVIPHPYAHERAFVLVPLSDLQLAREPWCTLGGRGILEWLEEVDPAERKAIVPADRSE
EEEEECCCCCCCCEEEEEECHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEECCCCCCC
LSVPGEGQSLGTNTRTKDEG
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]