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The map label for this gene is pck

Identifier: 68535216

GI number: 68535216

Start: 183533

End: 185353

Strand: Direct

Name: pck

Synonym: jk0151

Alternate gene names: 68535216

Gene position: 183533-185353 (Clockwise)

Preceding gene: 68535215

Following gene: 68535218

Centisome position: 7.45

GC content: 63.54

Gene sequence:

>1821_bases
ATGACCATTCGAGGGCTTGTCGGCGAGGCACCCACCAAGAACCAGGAGATGCTGAACTGGATCGAGGAGGCCGTGGAGCT
CTTCCAGCCAGAATCAGTCGTGTTCTGCGATGGCTCCGAGGAGGAGTGGAACGAGCTGGCAGAGCAGCTGGTCGAGCACG
GCACCCTGATCAAGCTGGACGAAGAGGCGCAGCCGAACTCCTTCCTGGCGCGCTCCAACCCGTCTGACGTTGCGCGCGTG
GAATCCCGCACCTTCATCTGCTCTAAGACTGAGGAAGACGCCGGCCCCACCAACAACTGGATGGACCCGGAGCAGATGCG
TGCCGAGATGCGCGAGCACTTCTCCGGCTCCATGAAGGGCCGCCGCATGTACATCGTGCCTTTCTGCATGGGCCCGATTA
CCGACCCGTCCCCGAAGCTGGGTATCGAAATCACCGACTCCCCGTACGTTGTTATGTCCATGCGCATCATGACCCGTATG
GGTAAGGAAGCTCTGGACAAGATCGGCGAGAACGGCGAGTTCGTGAAGGGTCTGCACTCCGTGGGCGCCCCGCTGGAGCC
GGGTCAGGAAGACTCGACCTGGCCGTGCAATGACACGAAGTACATCACCCACTTCCCGGAGGACCGCGAGATCTGGTCCT
ACGGCTCCGGCTACGGTGGCAACGCCATCCTGGCTAAGAAGTGCTACGCGCTGCGCATCGCTTCCGCCATGGCGCGCGAC
GAGGGCTGGATGGCAGAGCACATGCTGATCCTGAAGCTGATCAGCCCGGAGGACAAGGCCTACTACATCTGTGCTGCCTT
CCCATCCGCTTGTGGCAAGACCAACCTGGCCATGATCCAGCCGACCATCCCGGGCTGGCGCGCAGAGGTCGTCGGCGACG
ATATCGCATGGCTGCACTTCGGCGAGGACGGCCGCCTGTACGCAGTGAACCCGGAGAATGGTTTCTTCGGCGTGGCACCA
GGCACCAACTACGCTTCCAACCCGATGGCCATGAAGTCCATGGAGCCGGGCAACACCCTGTACACCAACGTGGCCCTGAC
TGACGACAACAACGTCTGGTGGGAGTCCAAGGAAGGCGAGCCGCAGCACCTCATCGACTGGCTGGGCAACGAGTGGACCC
CGGATTCCGGCAACAAGGCCGCGCACCCGAACTCCCGCTACTGCGTGCCGATCGAGCAGTGCCCGGTTGCTGCACCGGAG
TTCAACGACCCGAAGGGCGTGCCGGTTTCCGCCATCCTGTTCGGCGGTCGCCGTGCGGACACTGTGCCGCTGGTGACCCA
GGCTCGCGACTGGAACCACGCCACCTTCATCGGCGCCACCCTGGCTTCCGGCCAGACCGCCGCTGCTGCGGAGGCCGCCG
TTGGCTCCCTGCGTCACGACCCGATGGCCATGCTGCCGTTCATCGGCTACAACGCCGGTGACTACCTGCAGCACTGGATC
GACATGGGCAATAAGGGCGGCGACAAGATGCCAGAGGTCTTCCTGGTCAACTGGTTCCGCCGTGGTGAGGACGGCCGTTT
CCTGTGGCCGGGCTTCGGCGAGAACTCCCGCGTGCTGAAGTGGATCATCGACCGCATCGAGGGCCGCGTAGAGGCCGACG
AGACCGTCGTTGGCTACACCGCCCGCTACGAGGACATCTACACCGATGGCCTGAAGGAGACCGAGGAGGACATCCGCGAG
GCACTGTCCGTGAACCCGGCTGACTGGGAGCGCGACCTGGCCGACAATGAGGAGTGGCTGAAGTTCCTGGGCCCGAAGGT
TCCGTCCGAGGTATGGGACGAGTTCCAGGGTCTGAAGGATCGCGTGGAGGCTGCGAAGTAG

Upstream 100 bases:

>100_bases
GGTTTGAACTGGACGTTTAAACCGACGGTCGGCTGGTGCATGTTTGTGCGCCTTTTGGTCGGGCCGGGTTGTGGGACACA
AAAACGTAGGGAGATAATCG

Downstream 100 bases:

>100_bases
CTGCTAGTTGCGCTAGACGCTGCGCCTGATGGCGCTGCGTAGCGCTAGTAGCTCGCGTGCAGTGCTGGTAGCTCGCGTCC
GCGAGCGTGCTGGTGCTAGG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTIRGLVGEAPTKNQEMLNWIEEAVELFQPESVVFCDGSEEEWNELAEQLVEHGTLIKLDEEAQPNSFLARSNPSDVARV
ESRTFICSKTEEDAGPTNNWMDPEQMRAEMREHFSGSMKGRRMYIVPFCMGPITDPSPKLGIEITDSPYVVMSMRIMTRM
GKEALDKIGENGEFVKGLHSVGAPLEPGQEDSTWPCNDTKYITHFPEDREIWSYGSGYGGNAILAKKCYALRIASAMARD
EGWMAEHMLILKLISPEDKAYYICAAFPSACGKTNLAMIQPTIPGWRAEVVGDDIAWLHFGEDGRLYAVNPENGFFGVAP
GTNYASNPMAMKSMEPGNTLYTNVALTDDNNVWWESKEGEPQHLIDWLGNEWTPDSGNKAAHPNSRYCVPIEQCPVAAPE
FNDPKGVPVSAILFGGRRADTVPLVTQARDWNHATFIGATLASGQTAAAAEAAVGSLRHDPMAMLPFIGYNAGDYLQHWI
DMGNKGGDKMPEVFLVNWFRRGEDGRFLWPGFGENSRVLKWIIDRIEGRVEADETVVGYTARYEDIYTDGLKETEEDIRE
ALSVNPADWERDLADNEEWLKFLGPKVPSEVWDEFQGLKDRVEAAK

Sequences:

>Translated_606_residues
MTIRGLVGEAPTKNQEMLNWIEEAVELFQPESVVFCDGSEEEWNELAEQLVEHGTLIKLDEEAQPNSFLARSNPSDVARV
ESRTFICSKTEEDAGPTNNWMDPEQMRAEMREHFSGSMKGRRMYIVPFCMGPITDPSPKLGIEITDSPYVVMSMRIMTRM
GKEALDKIGENGEFVKGLHSVGAPLEPGQEDSTWPCNDTKYITHFPEDREIWSYGSGYGGNAILAKKCYALRIASAMARD
EGWMAEHMLILKLISPEDKAYYICAAFPSACGKTNLAMIQPTIPGWRAEVVGDDIAWLHFGEDGRLYAVNPENGFFGVAP
GTNYASNPMAMKSMEPGNTLYTNVALTDDNNVWWESKEGEPQHLIDWLGNEWTPDSGNKAAHPNSRYCVPIEQCPVAAPE
FNDPKGVPVSAILFGGRRADTVPLVTQARDWNHATFIGATLASGQTAAAAEAAVGSLRHDPMAMLPFIGYNAGDYLQHWI
DMGNKGGDKMPEVFLVNWFRRGEDGRFLWPGFGENSRVLKWIIDRIEGRVEADETVVGYTARYEDIYTDGLKETEEDIRE
ALSVNPADWERDLADNEEWLKFLGPKVPSEVWDEFQGLKDRVEAAK
>Mature_605_residues
TIRGLVGEAPTKNQEMLNWIEEAVELFQPESVVFCDGSEEEWNELAEQLVEHGTLIKLDEEAQPNSFLARSNPSDVARVE
SRTFICSKTEEDAGPTNNWMDPEQMRAEMREHFSGSMKGRRMYIVPFCMGPITDPSPKLGIEITDSPYVVMSMRIMTRMG
KEALDKIGENGEFVKGLHSVGAPLEPGQEDSTWPCNDTKYITHFPEDREIWSYGSGYGGNAILAKKCYALRIASAMARDE
GWMAEHMLILKLISPEDKAYYICAAFPSACGKTNLAMIQPTIPGWRAEVVGDDIAWLHFGEDGRLYAVNPENGFFGVAPG
TNYASNPMAMKSMEPGNTLYTNVALTDDNNVWWESKEGEPQHLIDWLGNEWTPDSGNKAAHPNSRYCVPIEQCPVAAPEF
NDPKGVPVSAILFGGRRADTVPLVTQARDWNHATFIGATLASGQTAAAAEAAVGSLRHDPMAMLPFIGYNAGDYLQHWID
MGNKGGDKMPEVFLVNWFRRGEDGRFLWPGFGENSRVLKWIIDRIEGRVEADETVVGYTARYEDIYTDGLKETEEDIREA
LSVNPADWERDLADNEEWLKFLGPKVPSEVWDEFQGLKDRVEAAK

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=618, Percent_Identity=47.411003236246, Blast_Score=572, Evalue=1e-163,
Organism=Homo sapiens, GI187281517, Length=618, Percent_Identity=46.6019417475728, Blast_Score=570, Evalue=1e-162,
Organism=Homo sapiens, GI66346723, Length=409, Percent_Identity=48.1662591687042, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17555492, Length=624, Percent_Identity=47.275641025641, Blast_Score=566, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989645, Length=620, Percent_Identity=47.0967741935484, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989658, Length=620, Percent_Identity=47.0967741935484, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989653, Length=595, Percent_Identity=47.7310924369748, Blast_Score=546, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564411, Length=545, Percent_Identity=49.9082568807339, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI32564407, Length=524, Percent_Identity=50.381679389313, Blast_Score=511, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71989649, Length=519, Percent_Identity=47.7842003853565, Blast_Score=492, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=370, Percent_Identity=47.2972972972973, Blast_Score=335, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17540956, Length=577, Percent_Identity=23.2235701906412, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24655085, Length=598, Percent_Identity=48.494983277592, Blast_Score=590, Evalue=1e-169,
Organism=Drosophila melanogaster, GI24655082, Length=610, Percent_Identity=48.8524590163934, Blast_Score=581, Evalue=1e-166,
Organism=Drosophila melanogaster, GI161077226, Length=568, Percent_Identity=49.6478873239437, Blast_Score=553, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24655088, Length=503, Percent_Identity=49.3041749502982, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CORJK (Q4JY04)

Other databases:

- EMBL:   CR931997
- RefSeq:   YP_249921.1
- ProteinModelPortal:   Q4JY04
- SMR:   Q4JY04
- STRING:   Q4JY04
- GeneID:   3432866
- GenomeReviews:   CR931997_GR
- KEGG:   cjk:jk0151
- NMPDR:   fig|306537.3.peg.225
- eggNOG:   COG1274
- HOGENOM:   HBG484437
- OMA:   WILERVE
- ProtClustDB:   PRK04210
- BioCyc:   CJEI306537:JK0151-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 67734; Mature: 67602

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 271-271 BINDING 79-79 BINDING 220-220 BINDING 227-227 BINDING 269-269 BINDING 386-386 BINDING 417-417

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRGLVGEAPTKNQEMLNWIEEAVELFQPESVVFCDGSEEEWNELAEQLVEHGTLIKLD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEC
EEAQPNSFLARSNPSDVARVESRTFICSKTEEDAGPTNNWMDPEQMRAEMREHFSGSMKG
CCCCCCCEEECCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
RRMYIVPFCMGPITDPSPKLGIEITDSPYVVMSMRIMTRMGKEALDKIGENGEFVKGLHS
CEEEEEEEECCCCCCCCCCCEEEECCCCEEEHHHHHHHHHCHHHHHHHCCCCHHHHHHHH
VGAPLEPGQEDSTWPCNDTKYITHFPEDREIWSYGSGYGGNAILAKKCYALRIASAMARD
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWMAEHMLILKLISPEDKAYYICAAFPSACGKTNLAMIQPTIPGWRAEVVGDDIAWLHF
CCHHHHHEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEE
GEDGRLYAVNPENGFFGVAPGTNYASNPMAMKSMEPGNTLYTNVALTDDNNVWWESKEGE
CCCCEEEEECCCCCEEECCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCEEEECCCCC
PQHLIDWLGNEWTPDSGNKAAHPNSRYCVPIEQCPVAAPEFNDPKGVPVSAILFGGRRAD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
TVPLVTQARDWNHATFIGATLASGQTAAAAEAAVGSLRHDPMAMLPFIGYNAGDYLQHWI
CCCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHEHHHCCCCHHHHHHHHH
DMGNKGGDKMPEVFLVNWFRRGEDGRFLWPGFGENSRVLKWIIDRIEGRVEADETVVGYT
HCCCCCCCCCCHHHEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
ARYEDIYTDGLKETEEDIREALSVNPADWERDLADNEEWLKFLGPKVPSEVWDEFQGLKD
EHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
RVEAAK
HHHHCC
>Mature Secondary Structure 
TIRGLVGEAPTKNQEMLNWIEEAVELFQPESVVFCDGSEEEWNELAEQLVEHGTLIKLD
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEC
EEAQPNSFLARSNPSDVARVESRTFICSKTEEDAGPTNNWMDPEQMRAEMREHFSGSMKG
CCCCCCCEEECCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
RRMYIVPFCMGPITDPSPKLGIEITDSPYVVMSMRIMTRMGKEALDKIGENGEFVKGLHS
CEEEEEEEECCCCCCCCCCCEEEECCCCEEEHHHHHHHHHCHHHHHHHCCCCHHHHHHHH
VGAPLEPGQEDSTWPCNDTKYITHFPEDREIWSYGSGYGGNAILAKKCYALRIASAMARD
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWMAEHMLILKLISPEDKAYYICAAFPSACGKTNLAMIQPTIPGWRAEVVGDDIAWLHF
CCHHHHHEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCEEEEEE
GEDGRLYAVNPENGFFGVAPGTNYASNPMAMKSMEPGNTLYTNVALTDDNNVWWESKEGE
CCCCEEEEECCCCCEEECCCCCCCCCCCCEECCCCCCCEEEEEEEEECCCCEEEECCCCC
PQHLIDWLGNEWTPDSGNKAAHPNSRYCVPIEQCPVAAPEFNDPKGVPVSAILFGGRRAD
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCCEEEEEECCCCCC
TVPLVTQARDWNHATFIGATLASGQTAAAAEAAVGSLRHDPMAMLPFIGYNAGDYLQHWI
CCCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHEHHHCCCCHHHHHHHHH
DMGNKGGDKMPEVFLVNWFRRGEDGRFLWPGFGENSRVLKWIIDRIEGRVEADETVVGYT
HCCCCCCCCCCHHHEEHHHHCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEE
ARYEDIYTDGLKETEEDIREALSVNPADWERDLADNEEWLKFLGPKVPSEVWDEFQGLKD
EHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHH
RVEAAK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA