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The map label for this gene is fadD1 [H]

Identifier: 68535202

GI number: 68535202

Start: 161506

End: 163395

Strand: Direct

Name: fadD1 [H]

Synonym: jk0137

Alternate gene names: 68535202

Gene position: 161506-163395 (Clockwise)

Preceding gene: 68535201

Following gene: 68535203

Centisome position: 6.56

GC content: 64.39

Gene sequence:

>1890_bases
GTGGACATCACTGCAGCAATGGGCCAGTTTTACGACGAGCAAGGCGACATTAATGTGCCCGAGCAGCTCACCCTTTCCGG
CATGTGCGAGATGCTTTACACCATGGCGCAGATGGAGGGCACGGTGGATAACGTGCTGATCCGCCACTGGGACTTCAGCG
AGTCCCGCGAGGGTACGGTGCACGAGATCACCCGCGCGCAGGTAAACACCCGCATCAAGGCCGTCTGCGTTCGCCTGCAG
CAGGTCGCCAAGCCTGGCGACCGCGTGGCCATCCTGGCCAACAACTCCCCGGAGTACATGTACGCCTTCCTGGGTGCGAT
GTACGCCCGCATGGTGCCCGTCCCACTGTATGACCCGAACGAGCCGGGCCACACTGGCCACCTGACTGCCGTATTGGGTT
CCTCTGAGCCGACGGTGGTGCTGACGAATAAGCGCTCCGCTACGGCCGTACGCAAGCACTTCGCCTCTACGCCCACCACT
GAGCGCCCCCGCGTGCTGACGGTAGACGCGCTGCCGGATGCGCTGGCTGACGAATGGCAGAACCCCATGGCCGCCATCAT
GGCGGACCCGTCGCTGGCGCCGAAGTCCAGCGAGGAAGCGTTCCTGCAGTACACTTCCGGCTCCACCCGCACCCCGGCTG
GCGTGGTGCTTACTCACAAGTCGATCGTCACCAACGTGCTGCAGATCTTCAAGGCGGCGAACCTGCAGTCTCCGCTGCGC
CTGAGCACTTGGTTGCCGCTGCACCACGACATGGGCGTGATCCTTTCCGCCTTTGTGATCATCCTGGGCCTACCTTTCGA
CCTGATGTCCCCGCGTGACTTCATCCAGGATCCGGCTCGCTGGGTTCGCCAGCTAAACCGCAAGAACGAGGAAGAGAACG
TCTACACCGTTGTGCCTAACTTCGCCCTGGAGTTGGCCGTGCGCTACGCGGACCCGGCGAAGGTCGCGGAGCTGGCTGAC
CTGGACCTATCTGCCGTCGACGGGATCATCAACGGTTCTGAGCCGGTCCACCACAGCTCTGTGCAGAAGTTCCTGGAAAC
CTTCGGCCCCTACGGCTTCAAGAAGGAAGCCATGCGCCCGTCCTACGGCCTGGCCGAGGCCACGCTGCTCGTCAGCACCC
CGCAGACCGACGAGCGTCCACTGACGAAGTGGTTCAAGCGCGAGGAGCTCTCCGATGGCACCGCCGTGGAAGGCAGTGCG
GACGATGCAGACAGCCTATCCCTGGTCTCTCTGGGTCAGGTGTGCCTCCCGCAGACTCTCGCAATCGTCGACCCGGAAAC
CGGCAAGGAGCTGACCGACGGCAACGTCGGTGAGATTTGGGTTCACGGCGACAACATGGCCGCGGGTTACCTAAACCGCC
CGGACGAGACCAAGGAGACGTTCCGCAACTCCCTGCCTACCGCCAACCGCCTGGATTCTGATAGCCGTGCGGGCGACGCT
TCAGAGGACAACTGGATGCGCACCGGTGACCTGGCCGTCATCGTCGATGGCCACCTGTTCATCACCGGCCGCCTGAAGGA
CCTCATCATCGTCGCAGGTCGTAACCACTACCCGCAGGACATTGAGGCCACCGCCGACGAAGCCACCGAGCAGACCGCCC
CGGCCGTCATCGCAGCCTTCGCCGTGGAGGCCGGTGGTTCCGCTAGCGGCGCCGCCGACGGTGAGTCTGTGGAGGGGCTC
GTCATCGTTGCTGAGCGTGACCCGGATGCCAGCGAGGACGGAGACGCGGAAGCGATCGAGGCCATGCGCGCGGCCGTTAC
CGCCACCCACGGCGTGCAGCCGTCTGACGTGCGCATCATCGCACCGGGCACCTTGCCGCGTTCTTCCGCCAACAAGATCG
CCCGCCGCGTGGCCGCGAAGGCGTATCTGGAGGGCACGCTGGGCGCTTAA

Upstream 100 bases:

>100_bases
AAGTGAAACTAAATGTTTATGGTGTTAGAGCTAAGGCCTTAGAGCTAGGTAGCTAACCCGATACCGTTTGAACCGAGTGG
AAAACTAGAGGAGACCCCGC

Downstream 100 bases:

>100_bases
TCCATAAAGCGACCTCGGCGCGTTAAAATGTAGCGCTACGGTGACTCACTCATGCCTTCATTAAGGCTTCCCGGTACGAA
AGGAAACGCGACCTTCCATG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MDITAAMGQFYDEQGDINVPEQLTLSGMCEMLYTMAQMEGTVDNVLIRHWDFSESREGTVHEITRAQVNTRIKAVCVRLQ
QVAKPGDRVAILANNSPEYMYAFLGAMYARMVPVPLYDPNEPGHTGHLTAVLGSSEPTVVLTNKRSATAVRKHFASTPTT
ERPRVLTVDALPDALADEWQNPMAAIMADPSLAPKSSEEAFLQYTSGSTRTPAGVVLTHKSIVTNVLQIFKAANLQSPLR
LSTWLPLHHDMGVILSAFVIILGLPFDLMSPRDFIQDPARWVRQLNRKNEEENVYTVVPNFALELAVRYADPAKVAELAD
LDLSAVDGIINGSEPVHHSSVQKFLETFGPYGFKKEAMRPSYGLAEATLLVSTPQTDERPLTKWFKREELSDGTAVEGSA
DDADSLSLVSLGQVCLPQTLAIVDPETGKELTDGNVGEIWVHGDNMAAGYLNRPDETKETFRNSLPTANRLDSDSRAGDA
SEDNWMRTGDLAVIVDGHLFITGRLKDLIIVAGRNHYPQDIEATADEATEQTAPAVIAAFAVEAGGSASGAADGESVEGL
VIVAERDPDASEDGDAEAIEAMRAAVTATHGVQPSDVRIIAPGTLPRSSANKIARRVAAKAYLEGTLGA

Sequences:

>Translated_629_residues
MDITAAMGQFYDEQGDINVPEQLTLSGMCEMLYTMAQMEGTVDNVLIRHWDFSESREGTVHEITRAQVNTRIKAVCVRLQ
QVAKPGDRVAILANNSPEYMYAFLGAMYARMVPVPLYDPNEPGHTGHLTAVLGSSEPTVVLTNKRSATAVRKHFASTPTT
ERPRVLTVDALPDALADEWQNPMAAIMADPSLAPKSSEEAFLQYTSGSTRTPAGVVLTHKSIVTNVLQIFKAANLQSPLR
LSTWLPLHHDMGVILSAFVIILGLPFDLMSPRDFIQDPARWVRQLNRKNEEENVYTVVPNFALELAVRYADPAKVAELAD
LDLSAVDGIINGSEPVHHSSVQKFLETFGPYGFKKEAMRPSYGLAEATLLVSTPQTDERPLTKWFKREELSDGTAVEGSA
DDADSLSLVSLGQVCLPQTLAIVDPETGKELTDGNVGEIWVHGDNMAAGYLNRPDETKETFRNSLPTANRLDSDSRAGDA
SEDNWMRTGDLAVIVDGHLFITGRLKDLIIVAGRNHYPQDIEATADEATEQTAPAVIAAFAVEAGGSASGAADGESVEGL
VIVAERDPDASEDGDAEAIEAMRAAVTATHGVQPSDVRIIAPGTLPRSSANKIARRVAAKAYLEGTLGA
>Mature_629_residues
MDITAAMGQFYDEQGDINVPEQLTLSGMCEMLYTMAQMEGTVDNVLIRHWDFSESREGTVHEITRAQVNTRIKAVCVRLQ
QVAKPGDRVAILANNSPEYMYAFLGAMYARMVPVPLYDPNEPGHTGHLTAVLGSSEPTVVLTNKRSATAVRKHFASTPTT
ERPRVLTVDALPDALADEWQNPMAAIMADPSLAPKSSEEAFLQYTSGSTRTPAGVVLTHKSIVTNVLQIFKAANLQSPLR
LSTWLPLHHDMGVILSAFVIILGLPFDLMSPRDFIQDPARWVRQLNRKNEEENVYTVVPNFALELAVRYADPAKVAELAD
LDLSAVDGIINGSEPVHHSSVQKFLETFGPYGFKKEAMRPSYGLAEATLLVSTPQTDERPLTKWFKREELSDGTAVEGSA
DDADSLSLVSLGQVCLPQTLAIVDPETGKELTDGNVGEIWVHGDNMAAGYLNRPDETKETFRNSLPTANRLDSDSRAGDA
SEDNWMRTGDLAVIVDGHLFITGRLKDLIIVAGRNHYPQDIEATADEATEQTAPAVIAAFAVEAGGSASGAADGESVEGL
VIVAERDPDASEDGDAEAIEAMRAAVTATHGVQPSDVRIIAPGTLPRSSANKIARRVAAKAYLEGTLGA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=567, Percent_Identity=25.0440917107584, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI44888818, Length=572, Percent_Identity=25.1748251748252, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI225735629, Length=572, Percent_Identity=23.9510489510489, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45827692, Length=572, Percent_Identity=23.9510489510489, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI225735625, Length=572, Percent_Identity=23.9510489510489, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1788107, Length=598, Percent_Identity=25.9197324414716, Blast_Score=113, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=563, Percent_Identity=23.2682060390764, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24654656, Length=581, Percent_Identity=24.2685025817556, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24581924, Length=338, Percent_Identity=24.5562130177515, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 68015; Mature: 68015

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITAAMGQFYDEQGDINVPEQLTLSGMCEMLYTMAQMEGTVDNVLIRHWDFSESREGTV
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCCCHH
HEITRAQVNTRIKAVCVRLQQVAKPGDRVAILANNSPEYMYAFLGAMYARMVPVPLYDPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
EPGHTGHLTAVLGSSEPTVVLTNKRSATAVRKHFASTPTTERPRVLTVDALPDALADEWQ
CCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHC
NPMAAIMADPSLAPKSSEEAFLQYTSGSTRTPAGVVLTHKSIVTNVLQIFKAANLQSPLR
CCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
LSTWLPLHHDMGVILSAFVIILGLPFDLMSPRDFIQDPARWVRQLNRKNEEENVYTVVPN
CEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
FALELAVRYADPAKVAELADLDLSAVDGIINGSEPVHHSSVQKFLETFGPYGFKKEAMRP
CEEEEEEECCCHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCC
SYGLAEATLLVSTPQTDERPLTKWFKREELSDGTAVEGSADDADSLSLVSLGQVCLPQTL
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEE
AIVDPETGKELTDGNVGEIWVHGDNMAAGYLNRPDETKETFRNSLPTANRLDSDSRAGDA
EEECCCCCCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SEDNWMRTGDLAVIVDGHLFITGRLKDLIIVAGRNHYPQDIEATADEATEQTAPAVIAAF
CCCCCEEECCEEEEEECEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEAGGSASGAADGESVEGLVIVAERDPDASEDGDAEAIEAMRAAVTATHGVQPSDVRII
HHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
APGTLPRSSANKIARRVAAKAYLEGTLGA
ECCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDITAAMGQFYDEQGDINVPEQLTLSGMCEMLYTMAQMEGTVDNVLIRHWDFSESREGTV
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCCCHH
HEITRAQVNTRIKAVCVRLQQVAKPGDRVAILANNSPEYMYAFLGAMYARMVPVPLYDPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
EPGHTGHLTAVLGSSEPTVVLTNKRSATAVRKHFASTPTTERPRVLTVDALPDALADEWQ
CCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHC
NPMAAIMADPSLAPKSSEEAFLQYTSGSTRTPAGVVLTHKSIVTNVLQIFKAANLQSPLR
CCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
LSTWLPLHHDMGVILSAFVIILGLPFDLMSPRDFIQDPARWVRQLNRKNEEENVYTVVPN
CEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCC
FALELAVRYADPAKVAELADLDLSAVDGIINGSEPVHHSSVQKFLETFGPYGFKKEAMRP
CEEEEEEECCCHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCC
SYGLAEATLLVSTPQTDERPLTKWFKREELSDGTAVEGSADDADSLSLVSLGQVCLPQTL
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEE
AIVDPETGKELTDGNVGEIWVHGDNMAAGYLNRPDETKETFRNSLPTANRLDSDSRAGDA
EEECCCCCCCCCCCCCCEEEEECCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SEDNWMRTGDLAVIVDGHLFITGRLKDLIIVAGRNHYPQDIEATADEATEQTAPAVIAAF
CCCCCEEECCEEEEEECEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
AVEAGGSASGAADGESVEGLVIVAERDPDASEDGDAEAIEAMRAAVTATHGVQPSDVRII
HHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEE
APGTLPRSSANKIARRVAAKAYLEGTLGA
ECCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]