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The map label for this gene is tagA [H]

Identifier: 68535136

GI number: 68535136

Start: 80309

End: 80989

Strand: Direct

Name: tagA [H]

Synonym: jk0074

Alternate gene names: 68535136

Gene position: 80309-80989 (Clockwise)

Preceding gene: 68535135

Following gene: 68535139

Centisome position: 3.26

GC content: 63.58

Gene sequence:

>681_bases
ATGGAAGGCCAACTTCTCACCACCAGCACCGGGCAGCTCGTCGATGAATCGGGGGTCGCGCGCCCGCCGTGGGCCTACCG
CAGCGAGATTGAGCTGGACTACTACGACAACGAGTGGGGCCGTCCGGTCATTACCGAGCATGGGTTGCTGGAGCGCATCG
CCCTCGAAGGTTTCCAATCCGGGCTGTCGTGGGCCACCATTCTGCGCAAGCGGCGGGACTTTCGCTCTGTCTTCTTTCTT
TTTGACGCCCAGCGCATCGCCGAAATGAGCGAGCCCCAAAGGCGTGCCGCTTTGCAGGACGAACGCTTGATCCGGAATGC
CCGCAAGCACGAGTCACTCTACGAAAACGCCAAGGCGACGCTGGAGCTGCGCCGAGCGGAGGAATTTCAGAAACTACCGG
AGGATTCGCCCGCGCGCGAGGTTCTGGGCGGGGCGGCCAACCGGTTAGCACCGGGGCTGCCGGTGCTCGTATGGTCGTTC
ACCCCGGCGGAGCACTCCCGACCGACATCGCTGGATGGGGTGCCTTCGTCCTCGCCGGAATCGAGGGCACTGGCGAAAGA
ACTGAAGCGCCGGGGGTTCCAGTTTGTCGGTCCCACTACCTGCTACGCGCTAATGCAAGCCATCGGCATGGTCGATGACC
GAGTGGTGGAGCCGCCCGCGGAAGCTAACCCTCTTGGGTAG

Upstream 100 bases:

>100_bases
CTCGCGGGCGCACTCCAAATTCCGCACTTCAGATCCCCGTACGCCAGACTTCATGTCACATGTTTGTCGCCTGTGTCTGG
CAGTAGTTGAAAGGAACCCG

Downstream 100 bases:

>100_bases
CCTAATCCGGGTAGCCCAGTCCGGGTAAGCCTGTGCTTTAGAGGCTTTAAAGGCTTAGAGTCGCGCGGCTAGCTCCACGG
CCTGGCGGATGGCACGCTTG

Product: DNA-3-methyladenine glycosidase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MEGQLLTTSTGQLVDESGVARPPWAYRSEIELDYYDNEWGRPVITEHGLLERIALEGFQSGLSWATILRKRRDFRSVFFL
FDAQRIAEMSEPQRRAALQDERLIRNARKHESLYENAKATLELRRAEEFQKLPEDSPAREVLGGAANRLAPGLPVLVWSF
TPAEHSRPTSLDGVPSSSPESRALAKELKRRGFQFVGPTTCYALMQAIGMVDDRVVEPPAEANPLG

Sequences:

>Translated_226_residues
MEGQLLTTSTGQLVDESGVARPPWAYRSEIELDYYDNEWGRPVITEHGLLERIALEGFQSGLSWATILRKRRDFRSVFFL
FDAQRIAEMSEPQRRAALQDERLIRNARKHESLYENAKATLELRRAEEFQKLPEDSPAREVLGGAANRLAPGLPVLVWSF
TPAEHSRPTSLDGVPSSSPESRALAKELKRRGFQFVGPTTCYALMQAIGMVDDRVVEPPAEANPLG
>Mature_226_residues
MEGQLLTTSTGQLVDESGVARPPWAYRSEIELDYYDNEWGRPVITEHGLLERIALEGFQSGLSWATILRKRRDFRSVFFL
FDAQRIAEMSEPQRRAALQDERLIRNARKHESLYENAKATLELRRAEEFQKLPEDSPAREVLGGAANRLAPGLPVLVWSF
TPAEHSRPTSLDGVPSSSPESRALAKELKRRGFQFVGPTTCYALMQAIGMVDDRVVEPPAEANPLG

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=198, Percent_Identity=36.8686868686869, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 25408; Mature: 25408

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQLLTTSTGQLVDESGVARPPWAYRSEIELDYYDNEWGRPVITEHGLLERIALEGFQS
CCCCEECCCCCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
GLSWATILRKRRDFRSVFFLFDAQRIAEMSEPQRRAALQDERLIRNARKHESLYENAKAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELRRAEEFQKLPEDSPAREVLGGAANRLAPGLPVLVWSFTPAEHSRPTSLDGVPSSSPE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
SRALAKELKRRGFQFVGPTTCYALMQAIGMVDDRVVEPPAEANPLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEGQLLTTSTGQLVDESGVARPPWAYRSEIELDYYDNEWGRPVITEHGLLERIALEGFQS
CCCCEECCCCCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
GLSWATILRKRRDFRSVFFLFDAQRIAEMSEPQRRAALQDERLIRNARKHESLYENAKAT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELRRAEEFQKLPEDSPAREVLGGAANRLAPGLPVLVWSFTPAEHSRPTSLDGVPSSSPE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCH
SRALAKELKRRGFQFVGPTTCYALMQAIGMVDDRVVEPPAEANPLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]