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The map label for this gene is cobB2 [H]

Identifier: 68535127

GI number: 68535127

Start: 71411

End: 72226

Strand: Direct

Name: cobB2 [H]

Synonym: jk0065

Alternate gene names: 68535127

Gene position: 71411-72226 (Clockwise)

Preceding gene: 68535125

Following gene: 68535130

Centisome position: 2.9

GC content: 66.3

Gene sequence:

>816_bases
ATGGTCAACGTGATCGCCAGCCCGCAGTCCACCTCCGACGATCTCCAGCCGCTGAAGTTGGAGGATCTGCCGGAGGATCT
TGTCGCCGCAGTGCGCCGCGCCAAGTATGTGGAAGTGTTCACCGGGGCGGGCATGTCCGCCGATTCCGGTTTGGATACCT
TTCGCGACGCGCAGACCGGCATCTGGTCGCATGTAGATCCCCAGGCGATGGCCACTTTGGATTCTTGGGCGAAGGATCCC
GAGCCCATGCTTGCCTGGTATCTGTGGCGGGCGGTGCTATGCCGCCGCGCAGAGGCAAACGCGGGGCACCGGGCGATAGG
TGAGTGGGCGGAACGAGGGGGCGTCACCGTACATGTAACGACCCAGAACATCGACGACCTGCACGAGCGTGGCGGCAGTA
AGAACGTGGCGCACCTGCATGGGAGCCTGTTTAAATACCGCTGCAGCATATGCAGCAAACCCGCGCGGATGCCGGATCTG
CCCGAGAAGCAGGTGGAACGCGCAACGCCGCCGACGTGCTCGCTGTGCGGGAATCCGGTGCGACCGGGCGTGGTGTGGTT
CGGCGAACCGTTGCCACACCGAGAATGGGAGGCCGCCGAGGAGGCTATGCGGGCGGCGGATGTCGTGGTGATCATCGGCA
CGTCGGGAGTGGTGTGGCCCGCCGCAGGACTGCCCGCGATTGCGGCCCAGCGCGGCACGCCTATCGTGGAAATCTCGCCC
GCGCGCACCGACCTGACGGATATGTGCACGTGGTCGCTGCGCACGACCGCAGCGAACGGGGCGCCGCTGCTGGTGGAGGC
GGCGGAGGGCGCCTAG

Upstream 100 bases:

>100_bases
TCTACCTCCAGCGCGTCGAAACTTTTTCGTAGCCTACCAGGCAATCTTCTTTTACGCCGGCTAGGCTTTTGAGCTTAACT
ATTCCAGCTAGGGTGGGGGC

Downstream 100 bases:

>100_bases
AGCGGCGCTGAGTCAGCCCCGCTAGAGACCTACTACAGCCCCGCTAGTTCTGCGGATTCGGAGGAGCCGGCTGGCTATTC
TTCGGCATCAGGCCGGCGTT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 2 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MVNVIASPQSTSDDLQPLKLEDLPEDLVAAVRRAKYVEVFTGAGMSADSGLDTFRDAQTGIWSHVDPQAMATLDSWAKDP
EPMLAWYLWRAVLCRRAEANAGHRAIGEWAERGGVTVHVTTQNIDDLHERGGSKNVAHLHGSLFKYRCSICSKPARMPDL
PEKQVERATPPTCSLCGNPVRPGVVWFGEPLPHREWEAAEEAMRAADVVVIIGTSGVVWPAAGLPAIAAQRGTPIVEISP
ARTDLTDMCTWSLRTTAANGAPLLVEAAEGA

Sequences:

>Translated_271_residues
MVNVIASPQSTSDDLQPLKLEDLPEDLVAAVRRAKYVEVFTGAGMSADSGLDTFRDAQTGIWSHVDPQAMATLDSWAKDP
EPMLAWYLWRAVLCRRAEANAGHRAIGEWAERGGVTVHVTTQNIDDLHERGGSKNVAHLHGSLFKYRCSICSKPARMPDL
PEKQVERATPPTCSLCGNPVRPGVVWFGEPLPHREWEAAEEAMRAADVVVIIGTSGVVWPAAGLPAIAAQRGTPIVEISP
ARTDLTDMCTWSLRTTAANGAPLLVEAAEGA
>Mature_271_residues
MVNVIASPQSTSDDLQPLKLEDLPEDLVAAVRRAKYVEVFTGAGMSADSGLDTFRDAQTGIWSHVDPQAMATLDSWAKDP
EPMLAWYLWRAVLCRRAEANAGHRAIGEWAERGGVTVHVTTQNIDDLHERGGSKNVAHLHGSLFKYRCSICSKPARMPDL
PEKQVERATPPTCSLCGNPVRPGVVWFGEPLPHREWEAAEEAMRAADVVVIIGTSGVVWPAAGLPAIAAQRGTPIVEISP
ARTDLTDMCTWSLRTTAANGAPLLVEAAEGA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=231, Percent_Identity=38.5281385281385, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI6912664, Length=250, Percent_Identity=36.4, Blast_Score=140, Evalue=9e-34,
Organism=Homo sapiens, GI13787215, Length=243, Percent_Identity=37.4485596707819, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI6912662, Length=286, Percent_Identity=25.1748251748252, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI7657575, Length=215, Percent_Identity=27.906976744186, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI6912660, Length=219, Percent_Identity=29.6803652968037, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI13775602, Length=225, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI13775600, Length=225, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI63054862, Length=192, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7706712, Length=213, Percent_Identity=30.5164319248826, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI300797577, Length=240, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI308199517, Length=238, Percent_Identity=36.5546218487395, Blast_Score=121, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17541892, Length=208, Percent_Identity=29.8076923076923, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325242, Length=217, Percent_Identity=27.1889400921659, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648389, Length=202, Percent_Identity=30.1980198019802, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571445, Length=192, Percent_Identity=27.6041666666667, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137536, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24645650, Length=255, Percent_Identity=24.7058823529412, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 29251; Mature: 29251

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVIASPQSTSDDLQPLKLEDLPEDLVAAVRRAKYVEVFTGAGMSADSGLDTFRDAQTG
CCEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHC
IWSHVDPQAMATLDSWAKDPEPMLAWYLWRAVLCRRAEANAGHRAIGEWAERGGVTVHVT
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEE
TQNIDDLHERGGSKNVAHLHGSLFKYRCSICSKPARMPDLPEKQVERATPPTCSLCGNPV
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHCCCCC
RPGVVWFGEPLPHREWEAAEEAMRAADVVVIIGTSGVVWPAAGLPAIAAQRGTPIVEISP
CCCEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHCCCCEEEECC
ARTDLTDMCTWSLRTTAANGAPLLVEAAEGA
CCCCHHHHHHHHHEECCCCCCCEEEECCCCC
>Mature Secondary Structure
MVNVIASPQSTSDDLQPLKLEDLPEDLVAAVRRAKYVEVFTGAGMSADSGLDTFRDAQTG
CCEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHC
IWSHVDPQAMATLDSWAKDPEPMLAWYLWRAVLCRRAEANAGHRAIGEWAERGGVTVHVT
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEEE
TQNIDDLHERGGSKNVAHLHGSLFKYRCSICSKPARMPDLPEKQVERATPPTCSLCGNPV
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHCCCCC
RPGVVWFGEPLPHREWEAAEEAMRAADVVVIIGTSGVVWPAAGLPAIAAQRGTPIVEISP
CCCEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCEECCCCCCHHHHHCCCCEEEECC
ARTDLTDMCTWSLRTTAANGAPLLVEAAEGA
CCCCHHHHHHHHHEECCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]