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The map label for this gene is gyrB [H]

Identifier: 68535067

GI number: 68535067

Start: 5501

End: 7525

Strand: Direct

Name: gyrB [H]

Synonym: jk0005

Alternate gene names: 68535067

Gene position: 5501-7525 (Clockwise)

Preceding gene: 68535066

Following gene: 68535068

Centisome position: 0.22

GC content: 61.78

Gene sequence:

>2025_bases
GTGGCTGAACCACAGAAGCAGAGCGACTACGGCGCGAGTTCAATTACGATCCTCGAGGGCCTGGAGGCCGTTCGCAAGCG
CCCCGGTATGTACATCGGTTCCACCGGCGAGCGCGGCCTGCACCACTTGGTGTGGGAGGTTGTGGACAACTCGGTTGACG
AGGCCATGGCGGGTTACGCCAAGGAAGTCAGCGTAACGCTGCTGGCCGACGGTGGCGTGGAGGTAATCGACGACGGCCGT
GGTATTCCGGTGGAAGATCACCCGACCGGCCCGCCGACTGTGCAGGTTGTTATGACGCAACTGCACGCGGGCGGCAAGTT
CGACTCGGACTCCTATGCCGTTTCCGGTGGTCTGCACGGCGTGGGCATCTCGGTGGTTAACGCCCTGTCCACCCGCATGG
AAACTGAGATTAAGCGCGATGGGTACCTGTGGCGTCAGAACTTCAACGAGGCAGTGCCCGAAGAGCTGCAGCAGGTGAAG
AAGGCCCGCGGCACCGGCACGAAGCAACGCTTTTGGCCGGACCCGGAGATTTTCGAGACCGTAGAGTTCAACTTCGACAC
TGTGGCCAAGCGCCTGCAGGAGATGGCCTTCCTAAACAAGGGTCTGACCATCAAGCTTGTCGACGAGCGCGAGAACCTCG
AGGACGATGAGGAGCTGGAGGCTGCGGCAGAGGATGCAGCGGCGCCGAAGTCCACTGAAGAAAAGGCTGCCGAGGCCGAG
AAGAAGAAGGGCCCACGTACCCGCGTCTTCCACTATCCGGAGGGCCTGAAGGACTATGTTGCCTACATCAACCGTAAGCG
CACTGCGATTCACCCGACCATCATCTCCTTCGACGTGGCCGAGGAGGATCACGAGGTAGAGGTCGCGCTGCAGTGGAACA
GTGGCTTTAGCCAGTCCGTCCACACCTTCGCCAACACCATCAACACCTTCGAGGGCGGTACGCATGAGGAAGGCTTCCGC
GCAGCGCTGACCTCCCTGATGAACCGCTATGCCCGCGACCACAAGCTGTTGAAGGCCAAGGATCCGAAGCTTAGCGGTGA
CGATGTGCGTGAAGGTCTCGCCGCAGTTATCTCCGTGCGCGTCGGTGATCCGCAGTTCGAGGGTCAGACGAAGACCAAGC
TTGGCAACACCGAGATCAAGGGCTTTGTTCAGAGGGCCGTCAATGAGCACCTGGCGGATTGGCTGGATGCCAACCCGGCG
GAGGCGAAGGCCATCGTGGACAAGGCAGTGGCTTCCGCCCACGCCCGCGATGCTGCCCGCAAGGCCCGCGATATGGTGCG
CCGCAAGTCCGCCTCCGATATTGGCGGTCTGCCGGGCAAGCTTGCCGATTGCCGCTCCCGTGACCCGAAGCTCAGCGAGC
TGTACATCGTGGAGGGTGACTCCGCAGGTGGCTCCGCGAAGTCCGGCCGCGATTCTATGTACCAGGCCATCCTGCCGTTG
CGCGGCAAGATCCTGAACGTGGAGAAGGCGCGCATGGATAAGGTTCTGAAGAACAACGAGGTCCAGGCCATCATCACGGC
TCTGGGTACCGGCATCCACGACGAGTTCGACATCAAGAAGCTGCGTTACCACAAGATCGTGCTGATGGCCGACGCCGACG
TGGATGGCCAGCACATCGCCACGCTACTGCTGACGCTGCTGTTCCGCTTTATGCGTCCGCTGGTGGAGGAAGGCTACGTG
TACCTTGCTCAGCCGCCGCTGTACAAGCTCAAGTGGGCCAAGGGAGAGCCGGGCTTTGCCTTCAGCGACCGCGAGCGCGA
TGAGCAACTCGAGGAGGGCCTGGCGAAGGGCCGGAAGATCAACAAGGATGACGGCATCCAGCGCTACAAGGGTCTAGGTG
AGATGAACGCCAAGGAGCTGTGGGAGACCACCATGGATCCGACGGTGCGCACCCTGCGCCGCGTGGACCTGGAGGATGCC
GCCGCCGCCGACGAGATCTTCAGCGTGCTGATGGGCGACGACGTTGTCGCCCGCCGCAGCTTCATCACCAAGAACGCACG
CGACGTGCGCTTCCTTGATGTTTAA

Upstream 100 bases:

>100_bases
GTTCAAATAGCTCAAACGGCCTGTCCAATGGCACCTTTAGGCCCGCGACGCGCTAGAATGGGGTGGTTATTTACGCGCGA
GAAAAGGAGTTTGACCCACC

Downstream 100 bases:

>100_bases
TCGAGTGCTGTGAGCGATTCGAAACACCAAGCGAAAACCACTCGTACGCAGCCACGCCGGGAGCCGACCCTGCTGCCGGT
AGAGATGGCCGATGGCACCA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MAEPQKQSDYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYAKEVSVTLLADGGVEVIDDGR
GIPVEDHPTGPPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRMETEIKRDGYLWRQNFNEAVPEELQQVK
KARGTGTKQRFWPDPEIFETVEFNFDTVAKRLQEMAFLNKGLTIKLVDERENLEDDEELEAAAEDAAAPKSTEEKAAEAE
KKKGPRTRVFHYPEGLKDYVAYINRKRTAIHPTIISFDVAEEDHEVEVALQWNSGFSQSVHTFANTINTFEGGTHEEGFR
AALTSLMNRYARDHKLLKAKDPKLSGDDVREGLAAVISVRVGDPQFEGQTKTKLGNTEIKGFVQRAVNEHLADWLDANPA
EAKAIVDKAVASAHARDAARKARDMVRRKSASDIGGLPGKLADCRSRDPKLSELYIVEGDSAGGSAKSGRDSMYQAILPL
RGKILNVEKARMDKVLKNNEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGQHIATLLLTLLFRFMRPLVEEGYV
YLAQPPLYKLKWAKGEPGFAFSDRERDEQLEEGLAKGRKINKDDGIQRYKGLGEMNAKELWETTMDPTVRTLRRVDLEDA
AAADEIFSVLMGDDVVARRSFITKNARDVRFLDV

Sequences:

>Translated_674_residues
MAEPQKQSDYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYAKEVSVTLLADGGVEVIDDGR
GIPVEDHPTGPPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRMETEIKRDGYLWRQNFNEAVPEELQQVK
KARGTGTKQRFWPDPEIFETVEFNFDTVAKRLQEMAFLNKGLTIKLVDERENLEDDEELEAAAEDAAAPKSTEEKAAEAE
KKKGPRTRVFHYPEGLKDYVAYINRKRTAIHPTIISFDVAEEDHEVEVALQWNSGFSQSVHTFANTINTFEGGTHEEGFR
AALTSLMNRYARDHKLLKAKDPKLSGDDVREGLAAVISVRVGDPQFEGQTKTKLGNTEIKGFVQRAVNEHLADWLDANPA
EAKAIVDKAVASAHARDAARKARDMVRRKSASDIGGLPGKLADCRSRDPKLSELYIVEGDSAGGSAKSGRDSMYQAILPL
RGKILNVEKARMDKVLKNNEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGQHIATLLLTLLFRFMRPLVEEGYV
YLAQPPLYKLKWAKGEPGFAFSDRERDEQLEEGLAKGRKINKDDGIQRYKGLGEMNAKELWETTMDPTVRTLRRVDLEDA
AAADEIFSVLMGDDVVARRSFITKNARDVRFLDV
>Mature_673_residues
AEPQKQSDYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYAKEVSVTLLADGGVEVIDDGRG
IPVEDHPTGPPTVQVVMTQLHAGGKFDSDSYAVSGGLHGVGISVVNALSTRMETEIKRDGYLWRQNFNEAVPEELQQVKK
ARGTGTKQRFWPDPEIFETVEFNFDTVAKRLQEMAFLNKGLTIKLVDERENLEDDEELEAAAEDAAAPKSTEEKAAEAEK
KKGPRTRVFHYPEGLKDYVAYINRKRTAIHPTIISFDVAEEDHEVEVALQWNSGFSQSVHTFANTINTFEGGTHEEGFRA
ALTSLMNRYARDHKLLKAKDPKLSGDDVREGLAAVISVRVGDPQFEGQTKTKLGNTEIKGFVQRAVNEHLADWLDANPAE
AKAIVDKAVASAHARDAARKARDMVRRKSASDIGGLPGKLADCRSRDPKLSELYIVEGDSAGGSAKSGRDSMYQAILPLR
GKILNVEKARMDKVLKNNEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGQHIATLLLTLLFRFMRPLVEEGYVY
LAQPPLYKLKWAKGEPGFAFSDRERDEQLEEGLAKGRKINKDDGIQRYKGLGEMNAKELWETTMDPTVRTLRRVDLEDAA
AADEIFSVLMGDDVVARRSFITKNARDVRFLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=713, Percent_Identity=24.8246844319776, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI19913408, Length=704, Percent_Identity=25.8522727272727, Blast_Score=117, Evalue=4e-26,
Organism=Escherichia coli, GI48994957, Length=574, Percent_Identity=52.6132404181185, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=41.5384615384615, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17535065, Length=679, Percent_Identity=24.300441826215, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI212645845, Length=590, Percent_Identity=25.4237288135593, Blast_Score=119, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI212645657, Length=208, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=692, Percent_Identity=25, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=666, Percent_Identity=25.6756756756757, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74778; Mature: 74647

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPQKQSDYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHH
KEVSVTLLADGGVEVIDDGRGIPVEDHPTGPPTVQVVMTQLHAGGKFDSDSYAVSGGLHG
HHEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGISVVNALSTRMETEIKRDGYLWRQNFNEAVPEELQQVKKARGTGTKQRFWPDPEIFET
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VEFNFDTVAKRLQEMAFLNKGLTIKLVDERENLEDDEELEAAAEDAAAPKSTEEKAAEAE
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
KKKGPRTRVFHYPEGLKDYVAYINRKRTAIHPTIISFDVAEEDHEVEVALQWNSGFSQSV
HHCCCCCEEEECCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCEEEEEEEECCCCHHHH
HTFANTINTFEGGTHEEGFRAALTSLMNRYARDHKLLKAKDPKLSGDDVREGLAAVISVR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEE
VGDPQFEGQTKTKLGNTEIKGFVQRAVNEHLADWLDANPAEAKAIVDKAVASAHARDAAR
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KARDMVRRKSASDIGGLPGKLADCRSRDPKLSELYIVEGDSAGGSAKSGRDSMYQAILPL
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
RGKILNVEKARMDKVLKNNEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGQHIA
CCCEECHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHEEEEEEEEEECCCCCHHHHH
TLLLTLLFRFMRPLVEEGYVYLAQPPLYKLKWAKGEPGFAFSDRERDEQLEEGLAKGRKI
HHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
NKDDGIQRYKGLGEMNAKELWETTMDPTVRTLRRVDLEDAAAADEIFSVLMGDDVVARRS
CCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
FITKNARDVRFLDV
HHHCCCCCEEEECC
>Mature Secondary Structure 
AEPQKQSDYGASSITILEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNSVDEAMAGYA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCHHHHHHHHHH
KEVSVTLLADGGVEVIDDGRGIPVEDHPTGPPTVQVVMTQLHAGGKFDSDSYAVSGGLHG
HHEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCH
VGISVVNALSTRMETEIKRDGYLWRQNFNEAVPEELQQVKKARGTGTKQRFWPDPEIFET
HHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VEFNFDTVAKRLQEMAFLNKGLTIKLVDERENLEDDEELEAAAEDAAAPKSTEEKAAEAE
HCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
KKKGPRTRVFHYPEGLKDYVAYINRKRTAIHPTIISFDVAEEDHEVEVALQWNSGFSQSV
HHCCCCCEEEECCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCEEEEEEEECCCCHHHH
HTFANTINTFEGGTHEEGFRAALTSLMNRYARDHKLLKAKDPKLSGDDVREGLAAVISVR
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEE
VGDPQFEGQTKTKLGNTEIKGFVQRAVNEHLADWLDANPAEAKAIVDKAVASAHARDAAR
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
KARDMVRRKSASDIGGLPGKLADCRSRDPKLSELYIVEGDSAGGSAKSGRDSMYQAILPL
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHH
RGKILNVEKARMDKVLKNNEVQAIITALGTGIHDEFDIKKLRYHKIVLMADADVDGQHIA
CCCEECHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHEEEEEEEEEECCCCCHHHHH
TLLLTLLFRFMRPLVEEGYVYLAQPPLYKLKWAKGEPGFAFSDRERDEQLEEGLAKGRKI
HHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
NKDDGIQRYKGLGEMNAKELWETTMDPTVRTLRRVDLEDAAAADEIFSVLMGDDVVARRS
CCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
FITKNARDVRFLDV
HHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7981652 [H]