Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 161986615

GI number: 161986615

Start: 2336710

End: 2338449

Strand: Direct

Name: leuA [H]

Synonym: jk2002

Alternate gene names: 161986615

Gene position: 2336710-2338449 (Clockwise)

Preceding gene: 68537096

Following gene: 68537098

Centisome position: 94.89

GC content: 64.37

Gene sequence:

>1740_bases
ATGCCGCACGTGCGCTACCAACCCTTCTTCGAAGAGGTAGAGGAAATCACCCTGCCGGACCGCACCTGGCCGGACAAGGT
CATCGACCGCGCCCCGCAGTGGTGCGCCGTGGACCTGCGCGACGGCAACCAGGCGCTGATCGACCCGATGAGCCCCGAGC
GCAAGCGTCGCATGTTCGAACTGCTGGTGGACATGGGCTACAAGGAGATCGAGGTCGGCTTCCCGTCTGCCTCCCAGACC
GACTTCAACTTCGTCCGCGAGATCATCGAGAAGAACATGATCCCCGACGACGTCACCATCCAGGTGCTGGTTCAGGCGCG
CGAGCACCTGATCCGCCGCACCTTCGAAGCCTGCGAGGGCGCGAAGAACGTCATCGTCCACTTCTACAACTCCACCTCGA
AGCTGCAGCGCAAGGTTGTGTTCCGCAAGGACAAGGAGGCCATCAAGAAGCTGGCCACCGACGCAGCCGAGCTGATTAAG
ACCATCGCCAAGGACTACCCAGACACGAACTGGCGCTGGGAGTACTCCCCCGAGTCCTACACCGGCACCGAGGTCGCCTA
CGCCAAGGAAGTCTGCGACGCAGTCGTCGAGGTAATGGACCCGACACCGGAGAACCCGATCATCCTGAACCTGCCCTCGA
CCGTCGAGATGATCACCCCAAACGTTTACGCGGACTCCATCGAGTGGATGCACCGCAACCTGAACCGCCGCGACTCCATC
ATCCTGTCGCTGCACCCGCACAACGACCGCGGCACCGGCGTGGCAGCTGCCGAGCTGGGCTACATGGCCGGCGCCGACCG
CATCGAAGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCTGCCTGGTCACCCTCGGTCTGAACATGCTGACCC
AGGGCGTGGACCCGCAGATCGACTTCTCTGACATTGACCAGGTCCGCCGCACCGTCGAGTACTGCAACCAGCTGCGCGTG
CCGGAGCGCCACCCTTACGGCGGCGACCTGGTGTTCACCGCATTCTCCGGCTCCCACCAGGATGCGATCAACAAGGGCCT
GGATGCCATCGCGGAGAAGATCGACCCGGACGCCACCGCCGAGGAAGTCACCCAGGAGGCCATGGTCGAGAAGACCTGGG
AGGTCCCCTACCTGCCGATCGACCCGAAGGACGTCGGCCGTTCCTACGAAGCCGTGATCCGCGTGAACTCCCAGTCCGGC
AAGGGCGGCGTGGCCTACATCATGAAGACCGACCACAACCTGCAGCTGCCGCGCCCGATGCAGGTGGAGTTCTCCTCCGT
CGTGCAGGCCGTCACCGATGCCGAGGGCGGCGAGGTGAACCCGAAGGCCATGTGGGACATCTTCTCCGGCGAGTACCTGG
ACCGCGTCGCCCCGATCGAGACCATCTCCCTGACCGTCGACGGCGGCCAGAACGAGGGCGAAGAGGCCAAGGTCACCGGC
CAGATCGAGTTCAACGGCGAAGGCAAGACTGTGCAGGGCCGCGGCAACGGTCCGATCAACGCATACTGCAACGCGCTGGA
AGAGCTGGGCTACGACGTGGAGGTGCAGGAGTACAGCCAGCACGCCCGCACCTCGGGCGACGACGCTGAGGCCGCCGCAT
ACGTCCTGGCCGAAATCAATGGGGCGAAGTACTGGGGCGTGGGCATCGCCAGCTCCATTACCTACGCTTCGCTGAAGGCC
ATTACTTCCGCGGTGAACCGTTCGCAGGATGCCGCCGCTGGGGTTTCCCAGGGAGTCTAG

Upstream 100 bases:

>100_bases
GTCTAACGACACTTTCATCTCCGCACCCGCCCGCATCAACACCCCGAACGGCGAAATCCCCGAAGGCCAGCCCGCCTGGA
ACAAGCAGCGCAACTCCTCC

Downstream 100 bases:

>100_bases
GGTCCCGGGAACCGCGCAGGCTGCCAGCGGGGCGCGGTTCCCCGCTGGGTAGTATGTAGTCCATGAACGAGAACGATCAC
TCGGCCGCCCCCGGCCCCGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MPHVRYQPFFEEVEEITLPDRTWPDKVIDRAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVDMGYKEIEVGFPSASQT
DFNFVREIIEKNMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSKLQRKVVFRKDKEAIKKLATDAAELIK
TIAKDYPDTNWRWEYSPESYTGTEVAYAKEVCDAVVEVMDPTPENPIILNLPSTVEMITPNVYADSIEWMHRNLNRRDSI
ILSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGLNMLTQGVDPQIDFSDIDQVRRTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAINKGLDAIAEKIDPDATAEEVTQEAMVEKTWEVPYLPIDPKDVGRSYEAVIRVNSQSG
KGGVAYIMKTDHNLQLPRPMQVEFSSVVQAVTDAEGGEVNPKAMWDIFSGEYLDRVAPIETISLTVDGGQNEGEEAKVTG
QIEFNGEGKTVQGRGNGPINAYCNALEELGYDVEVQEYSQHARTSGDDAEAAAYVLAEINGAKYWGVGIASSITYASLKA
ITSAVNRSQDAAAGVSQGV

Sequences:

>Translated_579_residues
MPHVRYQPFFEEVEEITLPDRTWPDKVIDRAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVDMGYKEIEVGFPSASQT
DFNFVREIIEKNMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSKLQRKVVFRKDKEAIKKLATDAAELIK
TIAKDYPDTNWRWEYSPESYTGTEVAYAKEVCDAVVEVMDPTPENPIILNLPSTVEMITPNVYADSIEWMHRNLNRRDSI
ILSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGLNMLTQGVDPQIDFSDIDQVRRTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAINKGLDAIAEKIDPDATAEEVTQEAMVEKTWEVPYLPIDPKDVGRSYEAVIRVNSQSG
KGGVAYIMKTDHNLQLPRPMQVEFSSVVQAVTDAEGGEVNPKAMWDIFSGEYLDRVAPIETISLTVDGGQNEGEEAKVTG
QIEFNGEGKTVQGRGNGPINAYCNALEELGYDVEVQEYSQHARTSGDDAEAAAYVLAEINGAKYWGVGIASSITYASLKA
ITSAVNRSQDAAAGVSQGV
>Mature_578_residues
PHVRYQPFFEEVEEITLPDRTWPDKVIDRAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVDMGYKEIEVGFPSASQTD
FNFVREIIEKNMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSKLQRKVVFRKDKEAIKKLATDAAELIKT
IAKDYPDTNWRWEYSPESYTGTEVAYAKEVCDAVVEVMDPTPENPIILNLPSTVEMITPNVYADSIEWMHRNLNRRDSII
LSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGLNMLTQGVDPQIDFSDIDQVRRTVEYCNQLRVP
ERHPYGGDLVFTAFSGSHQDAINKGLDAIAEKIDPDATAEEVTQEAMVEKTWEVPYLPIDPKDVGRSYEAVIRVNSQSGK
GGVAYIMKTDHNLQLPRPMQVEFSSVVQAVTDAEGGEVNPKAMWDIFSGEYLDRVAPIETISLTVDGGQNEGEEAKVTGQ
IEFNGEGKTVQGRGNGPINAYCNALEELGYDVEVQEYSQHARTSGDDAEAAAYVLAEINGAKYWGVGIASSITYASLKAI
TSAVNRSQDAAAGVSQGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=539, Percent_Identity=24.6753246753247, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=576, Percent_Identity=47.5694444444444, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=577, Percent_Identity=46.7937608318891, Blast_Score=529, Evalue=1e-151,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 64285; Mature: 64154

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVRYQPFFEEVEEITLPDRTWPDKVIDRAPQWCAVDLRDGNQALIDPMSPERKRRMFE
CCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVDMGYKEIEVGFPSASQTDFNFVREIIEKNMIPDDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSKLQRKVVFRKDKEAIKKLATDAAELIKTIAKDYPDTNWRWEYSPESY
CCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
TGTEVAYAKEVCDAVVEVMDPTPENPIILNLPSTVEMITPNVYADSIEWMHRNLNRRDSI
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGLNMLTQGVDPQI
EEEEECCCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSDIDQVRRTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAINKGLDAIAEKIDPDATA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCH
EEVTQEAMVEKTWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYIMKTDHNLQLPRPM
HHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSSVVQAVTDAEGGEVNPKAMWDIFSGEYLDRVAPIETISLTVDGGQNEGEEAKVTG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCEEEEEEEEECCCCCCCCEEEEE
QIEFNGEGKTVQGRGNGPINAYCNALEELGYDVEVQEYSQHARTSGDDAEAAAYVLAEIN
EEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHEEHEEECC
GAKYWGVGIASSITYASLKAITSAVNRSQDAAAGVSQGV
CCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
PHVRYQPFFEEVEEITLPDRTWPDKVIDRAPQWCAVDLRDGNQALIDPMSPERKRRMFE
CCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVDMGYKEIEVGFPSASQTDFNFVREIIEKNMIPDDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSKLQRKVVFRKDKEAIKKLATDAAELIKTIAKDYPDTNWRWEYSPESY
CCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
TGTEVAYAKEVCDAVVEVMDPTPENPIILNLPSTVEMITPNVYADSIEWMHRNLNRRDSI
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGLNMLTQGVDPQI
EEEEECCCCCCCCCHHHHHHHHCCCHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCC
DFSDIDQVRRTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAINKGLDAIAEKIDPDATA
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCH
EEVTQEAMVEKTWEVPYLPIDPKDVGRSYEAVIRVNSQSGKGGVAYIMKTDHNLQLPRPM
HHHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSSVVQAVTDAEGGEVNPKAMWDIFSGEYLDRVAPIETISLTVDGGQNEGEEAKVTG
CHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCEEEEEEEEECCCCCCCCEEEEE
QIEFNGEGKTVQGRGNGPINAYCNALEELGYDVEVQEYSQHARTSGDDAEAAAYVLAEIN
EEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHEEHEEECC
GAKYWGVGIASSITYASLKAITSAVNRSQDAAAGVSQGV
CCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA