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The map label for this gene is pglA [H]

Identifier: 77761171

GI number: 77761171

Start: 2243107

End: 2244825

Strand: Reverse

Name: pglA [H]

Synonym: XC_1849

Alternate gene names: 77761171

Gene position: 2244825-2243107 (Counterclockwise)

Preceding gene: 66768171

Following gene: 66768163

Centisome position: 43.6

GC content: 64.57

Gene sequence:

>1719_bases
ATGCAACCGCACCCTCGCCTTCCCGCGCTCGCGCCACTCTCGCTCGCACTCCTCGGCCTTGCCACGTTTCAGGCCAGCGC
AGCACGCCCGCATGGGCTAGACGTTGCCGATACCGCGCACAGCATCTCCACCAGTTGGGGTGTCGTGCAGCAGCCCGCGC
TCCCCAGCCAGGTCTGCACAACGCTCAAGGCTGCGCTGGTGCCGGTTGCCGGCTCGCTGGATGCGCTCGACCAGGATCCC
ACCCAATCCAAACGCGACACCGCACGCCTGCAGGCCGCCATCGACGATTGCCCTGCCGGCAGCGCGGTGCGTCTGGTGGT
CGGCGATGGCGGAGAATCCGGCCTGTTGAGCGGCCCGCTCACCATCAAGAGCGGCGTGACGTTGTGGATCGATCGCGGCG
TGACCTTGTTCGGCTCGCGCAATCCGCAGGATTACGACAATGGCCTGGGCACCTGCGGCACCGCCACCACCGACAAGGCC
AAGTCCTGCAACCCGTTGATCCACGTCGCCGACACTGCCAAGAGCGCCATCGTGGGCGCCGGCAAGATCGACGGCCGCGG
TGGCAGCACGCTCACCGCGGGACCGAATGCCGGCAAGGCCAGCTGGTGGGATCTGGCCTATCTCAACGTCACCAAGGGCC
TGAGCCAGCACGTGCCACGGCTGCTGCAGATCGATGACAGCACCGACTTCACGCTGTACGACATCACCCTGGAAAACTCG
CCCAATTTCCACGTCACCACCGACAACGTGGTGGGCCTGACCGCCTGGGGCATCAAGATCCTGGCACCTAGCCTGGTGTA
TTCGCGCCCCGGCTACCACTGCCCTGCCGGCAGCACCCCGGACGTCAATCCGCACGCCAGCTGCTTCACCCCGGAAACCG
CCAAGAACACCGACGGCTTCGACCCTGGCCAATCCAAGAACGTGCTGGCCAAGAACACCGACGGCTTCGACCCTGGCCAA
TCCAAGAACGTGCTGCTGGCGTACTCGTATATCGGTACCGGCGATGACGGCGTGGCGATCAAGGCGCATGCCAAAAACAA
GCGCAGCATCGCCTCGGAAAACATGCTGTTCGCCTACAACCAGTTCTACTACACGCACGGTTTTTCGCTCGGCAGCGAAA
CCGATTCGGGGATGCGCCATATCGCCGTGCGCGGCTTGAGCATCGACGGCTTCAACGCCAACGACGTGCAGCGCGATCCC
TACTCGGCCAATGGCCTGCGTATCAAGTCCGATGCTTCGCGCGGCGGCCAGGTCTACGACATCAGATTCGAGAACATCTG
CATGCGCGGGGTTGCGCGGCCGCTGGTATTCGACGCCAACTACGCCAACCCGGCCACACGCGACCAGTTGCCGCGGTTCA
GCGGCATTGCACTGACCAATGTGCACTCCCTGGGTTCCAAGGCCTTCGGTGGCGGCGAATTGAGCTTCTACGGCTATCGC
GATGCGACCACCACCCTGCCCATCACCATGTCGCTGGACAACGTGGTGCTCGACGGCGGCCCGGTCGCCTTTGCGCAGCC
GCATTTTGGTGGACCGGCCAGCAACCCGGGCGCGGTGCACTTCACCTTCAAAGGCGGGCCGGTGAGCTTCTTTGATCAGC
TCACCGAGTCGGCGGCCAACGATGTGCAGTTGCAGGGCCGGCCCGGGCCGGGCGCGCCGTTGCAATGCGACGATGCCTTC
ATTGCGTACCACAGCGTGCTGCCCGACTCACCGATCTAA

Upstream 100 bases:

>100_bases
AGCGTGGCGTGCCATCGCAGCGACCTGCGCTTATTCCTTGCAGCACGCATCGCATCCCTTTTGTCACCTCTCTCCCACTT
CCCTCCCCCCGGAACCGCCG

Downstream 100 bases:

>100_bases
GCAACGCGGTGTCTGCATGCCTGTGGCGCTGCTGTGGCCGGCCTGGCCGCAGCAGCGGTTATTCATTCGGCGCCGCTGGC
TGCGCGCCAGCTGTGCCAGA

Product: polygalacturonase

Products: NA

Alternate protein names: PGA; Pectinase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MQPHPRLPALAPLSLALLGLATFQASAARPHGLDVADTAHSISTSWGVVQQPALPSQVCTTLKAALVPVAGSLDALDQDP
TQSKRDTARLQAAIDDCPAGSAVRLVVGDGGESGLLSGPLTIKSGVTLWIDRGVTLFGSRNPQDYDNGLGTCGTATTDKA
KSCNPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGKASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
PNFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHASCFTPETAKNTDGFDPGQSKNVLAKNTDGFDPGQ
SKNVLLAYSYIGTGDDGVAIKAHAKNKRSIASENMLFAYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNANDVQRDP
YSANGLRIKSDASRGGQVYDIRFENICMRGVARPLVFDANYANPATRDQLPRFSGIALTNVHSLGSKAFGGGELSFYGYR
DATTTLPITMSLDNVVLDGGPVAFAQPHFGGPASNPGAVHFTFKGGPVSFFDQLTESAANDVQLQGRPGPGAPLQCDDAF
IAYHSVLPDSPI

Sequences:

>Translated_572_residues
MQPHPRLPALAPLSLALLGLATFQASAARPHGLDVADTAHSISTSWGVVQQPALPSQVCTTLKAALVPVAGSLDALDQDP
TQSKRDTARLQAAIDDCPAGSAVRLVVGDGGESGLLSGPLTIKSGVTLWIDRGVTLFGSRNPQDYDNGLGTCGTATTDKA
KSCNPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGKASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
PNFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHASCFTPETAKNTDGFDPGQSKNVLAKNTDGFDPGQ
SKNVLLAYSYIGTGDDGVAIKAHAKNKRSIASENMLFAYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNANDVQRDP
YSANGLRIKSDASRGGQVYDIRFENICMRGVARPLVFDANYANPATRDQLPRFSGIALTNVHSLGSKAFGGGELSFYGYR
DATTTLPITMSLDNVVLDGGPVAFAQPHFGGPASNPGAVHFTFKGGPVSFFDQLTESAANDVQLQGRPGPGAPLQCDDAF
IAYHSVLPDSPI
>Mature_572_residues
MQPHPRLPALAPLSLALLGLATFQASAARPHGLDVADTAHSISTSWGVVQQPALPSQVCTTLKAALVPVAGSLDALDQDP
TQSKRDTARLQAAIDDCPAGSAVRLVVGDGGESGLLSGPLTIKSGVTLWIDRGVTLFGSRNPQDYDNGLGTCGTATTDKA
KSCNPLIHVADTAKSAIVGAGKIDGRGGSTLTAGPNAGKASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
PNFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHASCFTPETAKNTDGFDPGQSKNVLAKNTDGFDPGQ
SKNVLLAYSYIGTGDDGVAIKAHAKNKRSIASENMLFAYNQFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNANDVQRDP
YSANGLRIKSDASRGGQVYDIRFENICMRGVARPLVFDANYANPATRDQLPRFSGIALTNVHSLGSKAFGGGELSFYGYR
DATTTLPITMSLDNVVLDGGPVAFAQPHFGGPASNPGAVHFTFKGGPVSFFDQLTESAANDVQLQGRPGPGAPLQCDDAF
IAYHSVLPDSPI

Specific function: Contributes to the wilt disease production on tomato [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 60174; Mature: 60174

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPHPRLPALAPLSLALLGLATFQASAARPHGLDVADTAHSISTSWGVVQQPALPSQVCT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEECCCCCHHHHH
TLKAALVPVAGSLDALDQDPTQSKRDTARLQAAIDDCPAGSAVRLVVGDGGESGLLSGPL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCE
TIKSGVTLWIDRGVTLFGSRNPQDYDNGLGTCGTATTDKAKSCNPLIHVADTAKSAIVGA
EECCCEEEEEECCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEC
GKIDGRGGSTLTAGPNAGKASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
CEECCCCCCEEECCCCCCCCCEEEEEEEEHHHHHHHHCCEEEEECCCCCEEEEEEEECCC
PNFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHASCFTPETAKNTDGF
CCEEEECCCEEEEEECCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DPGQSKNVLAKNTDGFDPGQSKNVLLAYSYIGTGDDGVAIKAHAKNKRSIASENMLFAYN
CCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCHHHCCCCEEEEEE
QFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNANDVQRDPYSANGLRIKSDASRGGQVYD
EEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEE
IRFENICMRGVARPLVFDANYANPATRDQLPRFSGIALTNVHSLGSKAFGGGELSFYGYR
EEHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCEEEEEHHHCCCCCCCCCCEEEEEEC
DATTTLPITMSLDNVVLDGGPVAFAQPHFGGPASNPGAVHFTFKGGPVSFFDQLTESAAN
CCCEEEEEEEEECCEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
DVQLQGRPGPGAPLQCDDAFIAYHSVLPDSPI
CEEEECCCCCCCCCEECCCEEEEECCCCCCCC
>Mature Secondary Structure
MQPHPRLPALAPLSLALLGLATFQASAARPHGLDVADTAHSISTSWGVVQQPALPSQVCT
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEECCCCCHHHHH
TLKAALVPVAGSLDALDQDPTQSKRDTARLQAAIDDCPAGSAVRLVVGDGGESGLLSGPL
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCE
TIKSGVTLWIDRGVTLFGSRNPQDYDNGLGTCGTATTDKAKSCNPLIHVADTAKSAIVGA
EECCCEEEEEECCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEC
GKIDGRGGSTLTAGPNAGKASWWDLAYLNVTKGLSQHVPRLLQIDDSTDFTLYDITLENS
CEECCCCCCEEECCCCCCCCCEEEEEEEEHHHHHHHHCCEEEEECCCCCEEEEEEEECCC
PNFHVTTDNVVGLTAWGIKILAPSLVYSRPGYHCPAGSTPDVNPHASCFTPETAKNTDGF
CCEEEECCCEEEEEECCHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DPGQSKNVLAKNTDGFDPGQSKNVLLAYSYIGTGDDGVAIKAHAKNKRSIASENMLFAYN
CCCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCHHHCCCCEEEEEE
QFYYTHGFSLGSETDSGMRHIAVRGLSIDGFNANDVQRDPYSANGLRIKSDASRGGQVYD
EEEEEECCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEE
IRFENICMRGVARPLVFDANYANPATRDQLPRFSGIALTNVHSLGSKAFGGGELSFYGYR
EEHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCCCEEEEEHHHCCCCCCCCCCEEEEEEC
DATTTLPITMSLDNVVLDGGPVAFAQPHFGGPASNPGAVHFTFKGGPVSFFDQLTESAAN
CCCEEEEEEEEECCEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
DVQLQGRPGPGAPLQCDDAFIAYHSVLPDSPI
CEEEECCCCCCCCCEECCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193922 [H]