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The map label for this gene is eda [H]

Identifier: 66768294

GI number: 66768294

Start: 2385200

End: 2385859

Strand: Reverse

Name: eda [H]

Synonym: XC_1973

Alternate gene names: 66768294

Gene position: 2385859-2385200 (Counterclockwise)

Preceding gene: 66768295

Following gene: 77761180

Centisome position: 46.34

GC content: 68.79

Gene sequence:

>660_bases
ATGACGATTGCCCAGACCCAAAACACCGCTGAACAGCTGCTGCGCGACGCCGGCATCCTGCCGGTGGTGACCGTCGACAC
GCTGGACCAGGCGCGTCGCGTGGCCGACGCGCTGCTCGAAGGCGGCCTGCCTGCCATCGAACTGACCCTGCGCACGCCGG
TCGCGATCGAGGCGCTGGCGATGCTCAAGCGCGAACTGCCCAACATCGTGATCGGTGCCGGCACGGTGCTGAGCGAACGG
CAGCTGCGCCAGTCGGTGGATGCCGGTGCAGACTTCCTGGTCACGCCCGGTACGCCAGCGCCGCTGGCACGCCTGCTGGC
CGACGCGCCGATCCCGGCCGTGCCCGGCGCTGCCACCCCAACCGAACTGCTGACCCTGATGGGCTTGGGCTTCCGCGTGT
GCAAGCTGTTCCCGGCCACCGCTGTGGGCGGCCTGCAGATGCTCAAGGGCCTGGCCGGCCCGCTGTCTGAGCTCAAGCTC
TGCCCCACTGGCGGCATCAGCGAAGCCAATGCCGCCGAATTCCTGTCGCAGCCGAACGTGCTGTGCATCGGCGGCTCGTG
GATGGTGCCCAAGGACTGGCTGGCGCAAGGTCAGTGGGACAAGGTCAAGGCAAGCTCGGCCAAGGCTGCGTCAATCGTGC
GGCAGGTGCGTGCGGGCTGA

Upstream 100 bases:

>100_bases
GCCGATGCGGCCGCTGCCGCGGCGCCGCACGAGTCCAAGGACGCCTGATCCCCTTCCCTCTCTGCGATCGCCCAGGCGGT
CGCCGTCTCCAGGAGTTGCA

Downstream 100 bases:

>100_bases
TGTTCTGGGCTGCATCCCGCGTGTTGGGAAAGCCGAGTGGCTTGAGAAGGCCGGTTGGCTGACGAAAGTTGATTGGCTGA
GCAAAGCTGATCAGCTGGCG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIEALAMLKRELPNIVIGAGTVLSER
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISEANAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKASSAKAASIVRQVRAG

Sequences:

>Translated_219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIEALAMLKRELPNIVIGAGTVLSER
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISEANAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKASSAKAASIVRQVRAG
>Mature_218_residues
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIEALAMLKRELPNIVIGAGTVLSERQ
LRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLC
PTGGISEANAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKASSAKAASIVRQVRAG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=196, Percent_Identity=42.3469387755102, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI48994954, Length=193, Percent_Identity=28.4974093264249, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22775; Mature: 22644

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIEALA
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSERQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISEANAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKASSAKAASIVRQVRAG
EEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIEALA
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSERQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEECCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISEANAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKASSAKAASIVRQVRAG
EEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]