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The map label for this gene is pleD [H]

Identifier: 66768087

GI number: 66768087

Start: 2146873

End: 2148117

Strand: Reverse

Name: pleD [H]

Synonym: XC_1766

Alternate gene names: 66768087

Gene position: 2148117-2146873 (Counterclockwise)

Preceding gene: 66768088

Following gene: 66768086

Centisome position: 41.72

GC content: 65.06

Gene sequence:

>1245_bases
GTGCTGCTGGTGGAGAACTCGCGCACGTTCACCAGCATGCTGCGCGAGGCCATCGAGCAACGCGTGGAGCTGCCGGTCAC
GGTCGTATCGACCCTGGCCGAAGCCGCGCGCGTGCTGGACGAAGAAAGTGGCTGGTTCCTGGTCTTGACCGGACTGGTGC
TGGTGGACGGCGACCGCGACAAGGTGGTGGAGTTCTTCCTCTCGCGCAACCTGCCCACCGTGGTGGTCAGTGGCGTCTAC
GACGAAGACCTGCGCCAGCGCGTGCTGCAACAGCAGATCATCGATTACGTCCTCAAGAACGCGCCAGGCAGCATCGAATA
CCTCGCCTGGCTGGTGCAGCGTCTGGAGCGCAACCGCCGCATTGGCGCGCTGGTGGTGGACGACTCGTTGTCTGCACGCA
CCTATGCTGCGGCGTTGTTGTCGATGTATGGCTACCGCGTGGTGCTGGCTGCCGACGGCGCTGCCGGGCTGCAGGCGATC
GAACGCGACCCCGGCATCCGCCTGACCATCGTCGACCAGGAAATGCCCGGCATGGAAGGGGTGGAATTCACCCGCCGGCT
GCGTGCGATCCGCTCGCGCGACAAGGTGGCGGTGATCGGCATCTCCGGCAACAGCGATTCGTCGCTGATCCCGCGTTTTC
TCAAGAACGGCGCCAATGACTTCCTGCGCAAGCCGTTCTCGCGCGAAGAATTCTTCTGCCGCGTCTCGCAGAACGTGGAC
CAGCTGGAGCTGATCGGCACGCTGCAGGACCTGGCCACCCGTGACTTCCTCACCGGCCTGCCCAACAGGCGCTATTTCCT
GGAGCAGAGCCAGCGGGTGATTCCCAAGTTGCTCTCGGAAGACCAGACCGTCACCGCGGCGATGCTGGATATCGACCACT
TCAAGCACATCAACGACACCTGGGGCCATGAAGCCGGCGACCAGGCGCTGCGTGCGGTGGCAGCCTCGGTGTCCGGGCAC
GCCCGCCCGCAGGATCTGGTGGCCCGCTTCGGCGGGGAAGAATTCTGCCTGCTGGTTCCGGGGCTGGATGCCGCCAACGC
CACGGGTTATTTCGAAACCTTGCGCGAGCGCATCAGTGCCCTGCGCGTGCGTATCGGCGACGAGACGCTGAGCATGACCG
TGAGCATCGGCGTGTGCATCGCCACCCAGCGCGACCAGTCGCTGCATCACCTGCTGGGCGAAGCAGACAAATACCTCTAC
CTGGCCAAGGCCGGCGGCCGCAACCAGGTGCGCCTGGCCAGCTGA

Upstream 100 bases:

>100_bases
ACCGAGCCGTTAGCTGACCTGACGGTTCGATCCAGACGGCTGACCGATGTCCCCAGGCACCCTACATCGCAACAGTGAAA
GCGAAGTCCTGCCGCAGCGC

Downstream 100 bases:

>100_bases
GCCGCAGCGCTGGCTTGATCCAGATCAACGCGCATGGCGCGCGTGATGCCTATCGTGCGCCCATCGCATGGGGCAAGGCA
TATCGATGGCACTGCTGATC

Product: transcriptional regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLLVENSRTFTSMLREAIEQRVELPVTVVSTLAEAARVLDEESGWFLVLTGLVLVDGDRDKVVEFFLSRNLPTVVVSGVY
DEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIGALVVDDSLSARTYAAALLSMYGYRVVLAADGAAGLQAI
ERDPGIRLTIVDQEMPGMEGVEFTRRLRAIRSRDKVAVIGISGNSDSSLIPRFLKNGANDFLRKPFSREEFFCRVSQNVD
QLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVIPKLLSEDQTVTAAMLDIDHFKHINDTWGHEAGDQALRAVAASVSGH
ARPQDLVARFGGEEFCLLVPGLDAANATGYFETLRERISALRVRIGDETLSMTVSIGVCIATQRDQSLHHLLGEADKYLY
LAKAGGRNQVRLAS

Sequences:

>Translated_414_residues
MLLVENSRTFTSMLREAIEQRVELPVTVVSTLAEAARVLDEESGWFLVLTGLVLVDGDRDKVVEFFLSRNLPTVVVSGVY
DEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIGALVVDDSLSARTYAAALLSMYGYRVVLAADGAAGLQAI
ERDPGIRLTIVDQEMPGMEGVEFTRRLRAIRSRDKVAVIGISGNSDSSLIPRFLKNGANDFLRKPFSREEFFCRVSQNVD
QLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVIPKLLSEDQTVTAAMLDIDHFKHINDTWGHEAGDQALRAVAASVSGH
ARPQDLVARFGGEEFCLLVPGLDAANATGYFETLRERISALRVRIGDETLSMTVSIGVCIATQRDQSLHHLLGEADKYLY
LAKAGGRNQVRLAS
>Mature_414_residues
MLLVENSRTFTSMLREAIEQRVELPVTVVSTLAEAARVLDEESGWFLVLTGLVLVDGDRDKVVEFFLSRNLPTVVVSGVY
DEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRRIGALVVDDSLSARTYAAALLSMYGYRVVLAADGAAGLQAI
ERDPGIRLTIVDQEMPGMEGVEFTRRLRAIRSRDKVAVIGISGNSDSSLIPRFLKNGANDFLRKPFSREEFFCRVSQNVD
QLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVIPKLLSEDQTVTAAMLDIDHFKHINDTWGHEAGDQALRAVAASVSGH
ARPQDLVARFGGEEFCLLVPGLDAANATGYFETLRERISALRVRIGDETLSMTVSIGVCIATQRDQSLHHLLGEADKYLY
LAKAGGRNQVRLAS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=35.3293413173653, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=35.5029585798817, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082007, Length=169, Percent_Identity=35.5029585798817, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=38.3333333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=34.5454545454545, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=192, Percent_Identity=30.7291666666667, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787802, Length=206, Percent_Identity=32.0388349514563, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787541, Length=197, Percent_Identity=30.4568527918782, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=31.4465408805031, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788381, Length=159, Percent_Identity=29.559748427673, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=149, Percent_Identity=30.2013422818792, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081977, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46109; Mature: 46109

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLVENSRTFTSMLREAIEQRVELPVTVVSTLAEAARVLDEESGWFLVLTGLVLVDGDRD
CEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCHH
KVVEFFLSRNLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRR
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
IGALVVDDSLSARTYAAALLSMYGYRVVLAADGAAGLQAIERDPGIRLTIVDQEMPGMEG
EEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCH
VEFTRRLRAIRSRDKVAVIGISGNSDSSLIPRFLKNGANDFLRKPFSREEFFCRVSQNVD
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCH
QLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVIPKLLSEDQTVTAAMLDIDHFKHINDT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
WGHEAGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGLDAANATGYFETLRERISA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
LRVRIGDETLSMTVSIGVCIATQRDQSLHHLLGEADKYLYLAKAGGRNQVRLAS
HHHHCCCCCEEEEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCEEECC
>Mature Secondary Structure
MLLVENSRTFTSMLREAIEQRVELPVTVVSTLAEAARVLDEESGWFLVLTGLVLVDGDRD
CEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCHH
KVVEFFLSRNLPTVVVSGVYDEDLRQRVLQQQIIDYVLKNAPGSIEYLAWLVQRLERNRR
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
IGALVVDDSLSARTYAAALLSMYGYRVVLAADGAAGLQAIERDPGIRLTIVDQEMPGMEG
EEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCH
VEFTRRLRAIRSRDKVAVIGISGNSDSSLIPRFLKNGANDFLRKPFSREEFFCRVSQNVD
HHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCCH
QLELIGTLQDLATRDFLTGLPNRRYFLEQSQRVIPKLLSEDQTVTAAMLDIDHFKHINDT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
WGHEAGDQALRAVAASVSGHARPQDLVARFGGEEFCLLVPGLDAANATGYFETLRERISA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHH
LRVRIGDETLSMTVSIGVCIATQRDQSLHHLLGEADKYLYLAKAGGRNQVRLAS
HHHHCCCCCEEEEEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA