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The map label for this gene is truB

Identifier: 66767931

GI number: 66767931

Start: 1929318

End: 1930244

Strand: Direct

Name: truB

Synonym: XC_1607

Alternate gene names: 66767931

Gene position: 1929318-1930244 (Clockwise)

Preceding gene: 66767930

Following gene: 66767932

Centisome position: 37.47

GC content: 68.82

Gene sequence:

>927_bases
GTGAGACCCCGCATCACCTTCCGTCCGCTGCATGGCATTTTGTTGCTCGACAAGCCGGCCGGACTCAGCTCGAACAATGC
ACTGCAGGCCGCCCGCCGGCTGCTGCGTGCCGAGAAGGGCGGCCACACCGGCAGCCTGGATCCGCTGGCCACCGGCCTGC
TGCCGTTGTGCTTTGGCGAGGCCACCAAGATTGCCGGCCTGTTGCTGGGCTCGGCCAAGGCCTACGACGCCGAGATCGTG
CTGGGCGTCACCACCGATACCGACGATGCCGATGGGCAGCCGCTGCGTGAGCGCTCGGTACCTGCGCTCAGCGAGGCTGC
ATTGCAGGCGGCGCTGGCGCCGTTCATCGGCCGCATCCAGCAGCAGGCGCCGATCTATTCGGCGCTCAAGCAGGGCGGCG
AGCCGCTGTACGCCAAGGCGCGCCGTGGCGAGGTCATCGAGGCGCCGGTGCGCGAGGTCGAGGTGCATGCCATCACGTTG
ACCAGCTATGCGTCGCCGCGGTTGCGGTTGCGCGTGACCTGCGGCTCCGGCACCTATATCCGTAGCCTGGCGCGCGACCT
TGGCGAGGTGCTGGGCTGTGGCGCGCACATCGCCGCGCTGCGCCGCGTGTGGGTGGAGCCGTTCCGCACGCCAGAGATGA
TCACCCTGGAAGCGCTCACCGCGCTGGTCGAATCCGGCGCCGATGCGGCGCAATTGCTGCTGCCAGTGGCCGCCGGGCTG
AGCGATTTTGCGCAGATCACGCTCGACGCCACCTTGGCGGCGCGCTTTCGCATGGGGCAGCGGTTGCGCGATCCGGCATT
CCCGGAAGGGCAGGTTGCGGTGTTCGATGCGGACGGCAGCCCAGCCGGGCTGGGCCTTGTCGACGCGGACGGACGGCTGT
CGCCGCAGCGCCTATTCAACGGTCTGAACGCCGCTGCAGCTTGTTAA

Upstream 100 bases:

>100_bases
GCAGGATGCCGAGCCGCGCTGAGCGACGGCAGCACGTGGCCGCCGCTCCGCGCGGCCTTGACCATGCGGGCCGCATCGCG
CCCGAGCGAGCGTTGATCCC

Downstream 100 bases:

>100_bases
GGTTTAGTCAACTTGTTCCCGGGGCCGCGCGACGTTACAATTTTGCGGCCCCGATCGGGGCACCCTCAGCGCCAAGCGCG
CACATTCCTAGCAAACGGCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRPRITFRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERSVPALSEAALQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGEVIEAPVREVEVHAITL
TSYASPRLRLRVTCGSGTYIRSLARDLGEVLGCGAHIAALRRVWVEPFRTPEMITLEALTALVESGADAAQLLLPVAAGL
SDFAQITLDATLAARFRMGQRLRDPAFPEGQVAVFDADGSPAGLGLVDADGRLSPQRLFNGLNAAAAC

Sequences:

>Translated_308_residues
MRPRITFRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERSVPALSEAALQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGEVIEAPVREVEVHAITL
TSYASPRLRLRVTCGSGTYIRSLARDLGEVLGCGAHIAALRRVWVEPFRTPEMITLEALTALVESGADAAQLLLPVAAGL
SDFAQITLDATLAARFRMGQRLRDPAFPEGQVAVFDADGSPAGLGLVDADGRLSPQRLFNGLNAAAAC
>Mature_308_residues
MRPRITFRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGEATKIAGLLLGSAKAYDAEIV
LGVTTDTDDADGQPLRERSVPALSEAALQAALAPFIGRIQQQAPIYSALKQGGEPLYAKARRGEVIEAPVREVEVHAITL
TSYASPRLRLRVTCGSGTYIRSLARDLGEVLGCGAHIAALRRVWVEPFRTPEMITLEALTALVESGADAAQLLLPVAAGL
SDFAQITLDATLAARFRMGQRLRDPAFPEGQVAVFDADGSPAGLGLVDADGRLSPQRLFNGLNAAAAC

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35.9090909090909, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503337, Length=195, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI215599015, Length=195, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI2367200, Length=307, Percent_Identity=43.9739413680782, Blast_Score=230, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI17553978, Length=191, Percent_Identity=27.7486910994764, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=173, Percent_Identity=32.3699421965318, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=192, Percent_Identity=31.7708333333333, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364189, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364187, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364185, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364183, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI62471759, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17975520, Length=194, Percent_Identity=28.8659793814433, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_XANC8 (Q4UWA0)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_242693.1
- ProteinModelPortal:   Q4UWA0
- SMR:   Q4UWA0
- STRING:   Q4UWA0
- GeneID:   3380062
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_1607
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q4UWA0
- ProtClustDB:   PRK04642
- BioCyc:   XCAM314565:XC_1607-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32487; Mature: 32487

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRITFRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGQPLRERSVPALSEAALQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGEVIEAPVREVEVHAITLTSYASPRLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEECCCCCCEECCCHHHEEEEEEEEECCCCCCEEEEEEECCCHHH
RSLARDLGEVLGCGAHIAALRRVWVEPFRTPEMITLEALTALVESGADAAQLLLPVAAGL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCH
SDFAQITLDATLAARFRMGQRLRDPAFPEGQVAVFDADGSPAGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHC
GLNAAAAC
CCHHHHCC
>Mature Secondary Structure
MRPRITFRPLHGILLLDKPAGLSSNNALQAARRLLRAEKGGHTGSLDPLATGLLPLCFGE
CCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
ATKIAGLLLGSAKAYDAEIVLGVTTDTDDADGQPLRERSVPALSEAALQAALAPFIGRIQ
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QQAPIYSALKQGGEPLYAKARRGEVIEAPVREVEVHAITLTSYASPRLRLRVTCGSGTYI
HCCHHHHHHHHCCCCEEECCCCCCEECCCHHHEEEEEEEEECCCCCCEEEEEEECCCHHH
RSLARDLGEVLGCGAHIAALRRVWVEPFRTPEMITLEALTALVESGADAAQLLLPVAAGL
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCH
SDFAQITLDATLAARFRMGQRLRDPAFPEGQVAVFDADGSPAGLGLVDADGRLSPQRLFN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHC
GLNAAAAC
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA