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The map label for this gene is pilR [H]

Identifier: 66767852

GI number: 66767852

Start: 1837158

End: 1838252

Strand: Reverse

Name: pilR [H]

Synonym: XC_1528

Alternate gene names: 66767852

Gene position: 1838252-1837158 (Counterclockwise)

Preceding gene: 66767853

Following gene: 66767851

Centisome position: 35.7

GC content: 59.91

Gene sequence:

>1095_bases
GTGGAGGCAACCTTGGCAAGAACCCGAATACTCATTGTCGATGACGAAGACAGCATTCGTTTTGGCATGCGAGACTTCCT
GGAGTCGCGGGGCTATGGCGTTGTAGATGCTGACAGTTGCCAACGCGCGCGCGAACTCTTTCAAGCTTCGCCACCGGATG
TTGCGGTCATTGATTACCGTCTGCATGACGGCAGCGCCATCGACCTGCTCCGTGACTTTCGGCAAATCGATGCAGATGTG
CCGATGATTGTCTTGACGGCGTATGGGTCGATCGACCTGGCAGTGCAAGCCGTCAAGGAGGGCGCAGAGCAGTTCTTGAC
CAAGCCCATCGAAATGCCTGCGCTGCATGTGATCCTCAAGCGCCTGCTGGCCACACGACGGCTGCAGCGACAGCAGCAGG
TGGTAGCGACCCGTGACAGGCGGGAGCGGGTCAACCCACTGTCTGGCGGCAGCCCTGCAATCCGGGACCTGGCTGAGCAG
GCGAGCAAAGTAATGCACACCGACAGCCCGATCCTCATCCTGGGCGAAACCGGCACGGGCAAGAGCCTGCTCGCCAAATG
GCTGCACCATCATGGCATGCGCGCAGACGAGGCCTTCGTTGATCTCAATTGCGCGGGCCTGTCGCCCGATTTTCTGGAGA
CGGAACTATTCGGCCACGAGAAAGGCGCATTTACAGGTGCGACCGCGAGCAAGCAGGGAATGCTCGAGATCGCCGATGGC
GGCACAGTCTTCCTCGATGAGATCGGTGATGTCGACCCCCGCGTCCAACCCAAACTGCTCAAGGTGCTGGAGGAAAAGCG
CTTCCGTCGTCTGGGCGCAGTACGGGAACGCGAAGTCGACATCCGGCTGATTGCAGCCACGCATCATGATCTTGCCAGCA
AGGTGAAAGAAGGCACATTTCGGAGTGATCTGTACTTCCGCATCAGCAGCATTCCATTGACGATGCCTGCGTTGCGTGAT
CGCAAGGAAGATATCGTGCCGCTTGCACGCACGCTCCTCGCACGCTCGTTGGCCGACCCCAGCCGTACGCCGCAGTTGAC
CGATGACGCCGCGCTGGCGCTGCGCGAGTATGCGTGGCCCGGCAATATCCGTTAG

Upstream 100 bases:

>100_bases
CATCGACGTGCACAGCGAACGTCGGGGCAAGGCAGACAGCCCGCAGATTCTGTTGATGCGTGCGCAGCTGGCGTTTTAAG
CCCGTACCGCACAGCCCACC

Downstream 100 bases:

>100_bases
CTCCGTAATGTGCTTGAGCGCGCAGCATTGCTCTGCAATGACAACACCATCACCCGGCGCGACCTTCGCTTTGAGTCGAC
ATCGGCACAGGATGCACACG

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEATLARTRILIVDDEDSIRFGMRDFLESRGYGVVDADSCQRARELFQASPPDVAVIDYRLHDGSAIDLLRDFRQIDADV
PMIVLTAYGSIDLAVQAVKEGAEQFLTKPIEMPALHVILKRLLATRRLQRQQQVVATRDRRERVNPLSGGSPAIRDLAEQ
ASKVMHTDSPILILGETGTGKSLLAKWLHHHGMRADEAFVDLNCAGLSPDFLETELFGHEKGAFTGATASKQGMLEIADG
GTVFLDEIGDVDPRVQPKLLKVLEEKRFRRLGAVREREVDIRLIAATHHDLASKVKEGTFRSDLYFRISSIPLTMPALRD
RKEDIVPLARTLLARSLADPSRTPQLTDDAALALREYAWPGNIR

Sequences:

>Translated_364_residues
MEATLARTRILIVDDEDSIRFGMRDFLESRGYGVVDADSCQRARELFQASPPDVAVIDYRLHDGSAIDLLRDFRQIDADV
PMIVLTAYGSIDLAVQAVKEGAEQFLTKPIEMPALHVILKRLLATRRLQRQQQVVATRDRRERVNPLSGGSPAIRDLAEQ
ASKVMHTDSPILILGETGTGKSLLAKWLHHHGMRADEAFVDLNCAGLSPDFLETELFGHEKGAFTGATASKQGMLEIADG
GTVFLDEIGDVDPRVQPKLLKVLEEKRFRRLGAVREREVDIRLIAATHHDLASKVKEGTFRSDLYFRISSIPLTMPALRD
RKEDIVPLARTLLARSLADPSRTPQLTDDAALALREYAWPGNIR
>Mature_364_residues
MEATLARTRILIVDDEDSIRFGMRDFLESRGYGVVDADSCQRARELFQASPPDVAVIDYRLHDGSAIDLLRDFRQIDADV
PMIVLTAYGSIDLAVQAVKEGAEQFLTKPIEMPALHVILKRLLATRRLQRQQQVVATRDRRERVNPLSGGSPAIRDLAEQ
ASKVMHTDSPILILGETGTGKSLLAKWLHHHGMRADEAFVDLNCAGLSPDFLETELFGHEKGAFTGATASKQGMLEIADG
GTVFLDEIGDVDPRVQPKLLKVLEEKRFRRLGAVREREVDIRLIAATHHDLASKVKEGTFRSDLYFRISSIPLTMPALRD
RKEDIVPLARTLLARSLADPSRTPQLTDDAALALREYAWPGNIR

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=364, Percent_Identity=39.2857142857143, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1790437, Length=362, Percent_Identity=39.2265193370166, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790299, Length=367, Percent_Identity=38.6920980926431, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1788905, Length=357, Percent_Identity=37.5350140056022, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI87082117, Length=215, Percent_Identity=47.4418604651163, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1789087, Length=219, Percent_Identity=44.2922374429224, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1787583, Length=245, Percent_Identity=42.4489795918367, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789233, Length=234, Percent_Identity=40.1709401709402, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=42.4778761061947, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI87082152, Length=222, Percent_Identity=43.6936936936937, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1786524, Length=258, Percent_Identity=39.1472868217054, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=39.5833333333333, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87081858, Length=217, Percent_Identity=33.6405529953917, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786911, Length=122, Percent_Identity=39.344262295082, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787375, Length=104, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 40563; Mature: 40563

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATLARTRILIVDDEDSIRFGMRDFLESRGYGVVDADSCQRARELFQASPPDVAVIDYR
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEE
LHDGSAIDLLRDFRQIDADVPMIVLTAYGSIDLAVQAVKEGAEQFLTKPIEMPALHVILK
ECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RLLATRRLQRQQQVVATRDRRERVNPLSGGSPAIRDLAEQASKVMHTDSPILILGETGTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KSLLAKWLHHHGMRADEAFVDLNCAGLSPDFLETELFGHEKGAFTGATASKQGMLEIADG
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
GTVFLDEIGDVDPRVQPKLLKVLEEKRFRRLGAVREREVDIRLIAATHHDLASKVKEGTF
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEHHHHHHHHHHCCCC
RSDLYFRISSIPLTMPALRDRKEDIVPLARTLLARSLADPSRTPQLTDDAALALREYAWP
CCCCEEEEECCCEECHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
GNIR
CCCC
>Mature Secondary Structure
MEATLARTRILIVDDEDSIRFGMRDFLESRGYGVVDADSCQRARELFQASPPDVAVIDYR
CCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEEE
LHDGSAIDLLRDFRQIDADVPMIVLTAYGSIDLAVQAVKEGAEQFLTKPIEMPALHVILK
ECCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RLLATRRLQRQQQVVATRDRRERVNPLSGGSPAIRDLAEQASKVMHTDSPILILGETGTG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KSLLAKWLHHHGMRADEAFVDLNCAGLSPDFLETELFGHEKGAFTGATASKQGMLEIADG
HHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
GTVFLDEIGDVDPRVQPKLLKVLEEKRFRRLGAVREREVDIRLIAATHHDLASKVKEGTF
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEHHHHHHHHHHCCCC
RSDLYFRISSIPLTMPALRDRKEDIVPLARTLLARSLADPSRTPQLTDDAALALREYAWP
CCCCEEEEECCCEECHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
GNIR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]