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The map label for this gene is gcvA [H]

Identifier: 66767816

GI number: 66767816

Start: 1795458

End: 1796450

Strand: Reverse

Name: gcvA [H]

Synonym: XC_1490

Alternate gene names: 66767816

Gene position: 1796450-1795458 (Counterclockwise)

Preceding gene: 66767818

Following gene: 66767815

Centisome position: 34.89

GC content: 69.59

Gene sequence:

>993_bases
ATGCATCTGCGTCCTACCTTGCTGCCTGCACTCGGCGTCTTCGCCGCAGCCGCGCGCCACCAGAACTTCGCCCATGCGGC
GGAAGAACTGCACCTCACCGCCAGCGCGGTGAGCCATCACGTGCGCAAGCTCGAGGCACTGCTCGGTGCAACCTTGTTTC
AGCGGCTGCCGCGCGGCGTAATGCTCACCGCCGAAGGACGCCAGCTGGCCGATGCCGCCAGCGCGGCGCTGGCCGAGATC
GCCGCGGTGGCGGGCAATCTGCACCCGCGCGAAGACGCCATCCCGCTGCGCGTGACCACGCTGCGCTCGCTGTCGTATTG
CTGGTTGTTGCCGCGCCTGCCGCGGTTCTGCCGCCTGCACCCGCATATCCGCCTGGACCTGCAATCGGACCCGGCATTCT
CGCGCTTCGAGGAAGGCGGGCCGGAAGTGGGCATCCGCTACGGCCAAGGCGCCTGGCCGGGGTTGACCTCGCACCACCTG
ATGGACGACGAACTGTTCCCGGTGGCCTCGCCCTCGTTGCCCGAAGTGGCCGCGTTGCAGACGCCGGCGCAGATTGCGCA
ACTGCCGCTGTTGTCGGACATGTCGCCGCAGGGCTGGCGCGACTGGTTCCGCGCGGCGCAGGTGCGCGGCACCACGCTGC
CGCCGATGCACACCTTCAACGACAGCACCGATGCGATGCGCGCGGCCGTGTACGGACTGGGCGCGGTGCTGGCACGCAAG
CACATTGCCCAACCGTATCTGCAGCGCTATGAGCTGGTACGCCTGCCCGGCCCGACCTTGAAGGCGCGCTACGCCTACTA
CATCGTGCACCCCAGCCATCGCGCGCCCAGCCCGGCGGCCACCGTGTTCATCGACTGGCTCAAACGCGAAGCACTGGACG
AGCGCACGCCGATGCCGGCACTGCCCGCCGAACTGCTGGCATTGCCCACCGGCGAGACCGCGCACGCGCCGGACAGACGG
CGGCGATCGCGCCGTGCGCCGGCAACCGAGTGA

Upstream 100 bases:

>100_bases
TGGCGCGGGATCGAGATGGGGAGCGACAAAGCATCCGCGCACATCGCAGGCGCGACAAGCGCGTAGTATCGGGGCGAGCC
ACAAATCTCACTCATGCATC

Downstream 100 bases:

>100_bases
GCCTTCGCCGCAGGTAGTGCATCGCCATCTGCAACTGCGCCGCGATGTCTCCCGTTGTGCAAGCGATCCAATCAGTCTGC
CGCCCAGTGGCGCGTCAGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MHLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVMLTAEGRQLADAASAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRLHPHIRLDLQSDPAFSRFEEGGPEVGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALQTPAQIAQLPLLSDMSPQGWRDWFRAAQVRGTTLPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRAPSPAATVFIDWLKREALDERTPMPALPAELLALPTGETAHAPDRR
RRSRRAPATE

Sequences:

>Translated_330_residues
MHLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVMLTAEGRQLADAASAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRLHPHIRLDLQSDPAFSRFEEGGPEVGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALQTPAQIAQLPLLSDMSPQGWRDWFRAAQVRGTTLPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRAPSPAATVFIDWLKREALDERTPMPALPAELLALPTGETAHAPDRR
RRSRRAPATE
>Mature_330_residues
MHLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVMLTAEGRQLADAASAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRLHPHIRLDLQSDPAFSRFEEGGPEVGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALQTPAQIAQLPLLSDMSPQGWRDWFRAAQVRGTTLPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRAPSPAATVFIDWLKREALDERTPMPALPAELLALPTGETAHAPDRR
RRSRRAPATE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=33.2203389830508, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=36.3321799307958, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=33.7883959044369, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=26.0726072607261, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=29.7872340425532, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=25.7042253521127, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=24.5674740484429, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36430; Mature: 36430

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTAEGRQLADAASAALAEIAAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRLH
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCC
PHIRLDLQSDPAFSRFEEGGPEVGIRYGQGAWPGLTSHHLMDDELFPVASPSLPEVAALQ
CCEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
TPAQIAQLPLLSDMSPQGWRDWFRAAQVRGTTLPPMHTFNDSTDAMRAAVYGLGAVLARK
CHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRAPSPAATVFIDWLKREALDERTPMPA
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPAELLALPTGETAHAPDRRRRSRRAPATE
CCHHHHCCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MHLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
MLTAEGRQLADAASAALAEIAAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRLH
EEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCC
PHIRLDLQSDPAFSRFEEGGPEVGIRYGQGAWPGLTSHHLMDDELFPVASPSLPEVAALQ
CCEEEEECCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
TPAQIAQLPLLSDMSPQGWRDWFRAAQVRGTTLPPMHTFNDSTDAMRAAVYGLGAVLARK
CHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRAPSPAATVFIDWLKREALDERTPMPA
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPAELLALPTGETAHAPDRRRRSRRAPATE
CCHHHHCCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]