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The map label for this gene is murD

Identifier: 66767702

GI number: 66767702

Start: 1660894

End: 1662300

Strand: Direct

Name: murD

Synonym: XC_1375

Alternate gene names: 66767702

Gene position: 1660894-1662300 (Clockwise)

Preceding gene: 66767701

Following gene: 66767703

Centisome position: 32.26

GC content: 69.23

Gene sequence:

>1407_bases
GTGCGAATTTCGCAGCTTGAGGGCAAGGCGGTTGCGTTGTGGGGATGGGCGCGCGAAGGGCGCGCCGCCTACCGCGCGCT
GCGCCAACAGCTGCCTGCGCAGCCGCTGACGGTCTTTTGTAACGCCGAAGAGGCACGCGACGTTGCCGCATTGGCAGACC
CCGCGTTGCAGGTGCAGACCGAAGCGAGCGCACAGGCGCTGGCCGCGTTCGAGGTCGTGATCAAGTCACCGGGCATCAGC
CCGTACCGTGAGGAGGCACGCGCCGCCGCCGCGCAGGGCGCGCGCTTCATCGGCGGCACCGCGCTGTGGTTTGCCGAACA
CGCGCAGCCGGACGGCTACGTGCCGGGCGCGATCTGCGTGACCGGCACCAAGGGCAAGAGCACCACCACCGCGCTGCTGG
CGCATCTGTTGCGCGCCGACGGCCATCGCACCGCGCTGGTCGGCAATATCGGCCAGCCGTTGCTGGAAGTGCTCTCCCCA
CAGCCGCCGCCTGCGTACTGGGCGATCGAGCTGTCCAGCTACCAGACCGGTGAGGTGGGGCGCAGTGGCGCACGTCCGGA
GTTGGCGCTGGTGCTGAACCTGTTTCCCGAGCATCTGGACTGGCACGGCAGCGAAGCGGCGTATGTGCGCGACAAGCTGG
CGCTGGTGACTGACGGCCGCCCGCGCATCGCGCTGCTCAATGCCGCAGACCCGCATCTGGCGCAGCTGCAGCTGCCTGAG
AGCGAGGTGCGCTGGTTCAATCATCCCGACGGCTGGCACCTGCGTGGCGACGTGGTCTATCGCGGCCAACAACCGATCTT
CGATACCGCCAACGTGCCGCTGCCGGGGGAACACAACCGGCGCAACCTGTGCGCCGTGCTGGCGGCAGTGGAGGCCCTGG
GCCTGGATGCGGCGGCACTGGCGCCGGCGGCACTGACCTTTCGTCCGTTGCCCAACCGGCTGCAGTGGCTCGGCAGCGTG
GACGGCATCGCATACGTCAACGATTCCATCAGCACCACACCGCATGCCTCGCTGGCGGCCTTGGCCTGCTTCGCGCAGCA
GCGCGTGGCGTTGCTGGTGGGTGGGCACGACCGTGGGCTGGACTGGCAGGAATTCGCCGCGCACATGGCGCAGCAGGCGC
CGCTGGAAATCGTCACCATGGGCGCCAACGGCCCGCGCATCCATGCGTTGCTGGCACCACTGGCGCAGAGCGCCGGCTTT
GGCCTGCATGCCGCCGATGACCTGGCGCATGCGATGCAGCTGGCACGCAGCGCGCTCGGTGCACAGGGCGGGGTGCTGCT
GCTGTCGCCCGGCGCGCCCAGCTTTGGCGTGTACAGCGATTACGTGGCGCGTGGCCGCCACTTTGCGCAGCTGGCGGGGT
TCGATCCTGCGGCGATCAGTGCGATTCCCGGCCTGGGTGTGCAGTAG

Upstream 100 bases:

>100_bases
GAGATCCAACCGCAGCTGGAGCAGGACGCACTGCAGCTGCAGCCCCTGATGACCATCGATGGCGAGCACCGCGTTCCTTC
GGCGCTGTGGGAGCGCGTGC

Downstream 100 bases:

>100_bases
CTCCAAGCTGTCTTTCACGTTGCGCGTTTCGGGTTGCAAGCCTTCGGTCTGTTTTGTCGGAATGGATGGCGGGCCGGCTA
CGCGCTGCGCGCCGTCACCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRISQLEGKAVALWGWAREGRAAYRALRQQLPAQPLTVFCNAEEARDVAALADPALQVQTEASAQALAAFEVVIKSPGIS
PYREEARAAAAQGARFIGGTALWFAEHAQPDGYVPGAICVTGTKGKSTTTALLAHLLRADGHRTALVGNIGQPLLEVLSP
QPPPAYWAIELSSYQTGEVGRSGARPELALVLNLFPEHLDWHGSEAAYVRDKLALVTDGRPRIALLNAADPHLAQLQLPE
SEVRWFNHPDGWHLRGDVVYRGQQPIFDTANVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAALTFRPLPNRLQWLGSV
DGIAYVNDSISTTPHASLAALACFAQQRVALLVGGHDRGLDWQEFAAHMAQQAPLEIVTMGANGPRIHALLAPLAQSAGF
GLHAADDLAHAMQLARSALGAQGGVLLLSPGAPSFGVYSDYVARGRHFAQLAGFDPAAISAIPGLGVQ

Sequences:

>Translated_468_residues
MRISQLEGKAVALWGWAREGRAAYRALRQQLPAQPLTVFCNAEEARDVAALADPALQVQTEASAQALAAFEVVIKSPGIS
PYREEARAAAAQGARFIGGTALWFAEHAQPDGYVPGAICVTGTKGKSTTTALLAHLLRADGHRTALVGNIGQPLLEVLSP
QPPPAYWAIELSSYQTGEVGRSGARPELALVLNLFPEHLDWHGSEAAYVRDKLALVTDGRPRIALLNAADPHLAQLQLPE
SEVRWFNHPDGWHLRGDVVYRGQQPIFDTANVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAALTFRPLPNRLQWLGSV
DGIAYVNDSISTTPHASLAALACFAQQRVALLVGGHDRGLDWQEFAAHMAQQAPLEIVTMGANGPRIHALLAPLAQSAGF
GLHAADDLAHAMQLARSALGAQGGVLLLSPGAPSFGVYSDYVARGRHFAQLAGFDPAAISAIPGLGVQ
>Mature_468_residues
MRISQLEGKAVALWGWAREGRAAYRALRQQLPAQPLTVFCNAEEARDVAALADPALQVQTEASAQALAAFEVVIKSPGIS
PYREEARAAAAQGARFIGGTALWFAEHAQPDGYVPGAICVTGTKGKSTTTALLAHLLRADGHRTALVGNIGQPLLEVLSP
QPPPAYWAIELSSYQTGEVGRSGARPELALVLNLFPEHLDWHGSEAAYVRDKLALVTDGRPRIALLNAADPHLAQLQLPE
SEVRWFNHPDGWHLRGDVVYRGQQPIFDTANVPLPGEHNRRNLCAVLAAVEALGLDAAALAPAALTFRPLPNRLQWLGSV
DGIAYVNDSISTTPHASLAALACFAQQRVALLVGGHDRGLDWQEFAAHMAQQAPLEIVTMGANGPRIHALLAPLAQSAGF
GLHAADDLAHAMQLARSALGAQGGVLLLSPGAPSFGVYSDYVARGRHFAQLAGFDPAAISAIPGLGVQ

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=393, Percent_Identity=26.972010178117, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_XANC8 (Q4UWX9)

Other databases:

- EMBL:   CP000050
- RefSeq:   YP_242464.1
- ProteinModelPortal:   Q4UWX9
- STRING:   Q4UWX9
- GeneID:   3382313
- GenomeReviews:   CP000050_GR
- KEGG:   xcb:XC_1375
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   LWVDDSK
- ProtClustDB:   PRK04690
- BioCyc:   XCAM314565:XC_1375-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49586; Mature: 49586

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISQLEGKAVALWGWAREGRAAYRALRQQLPAQPLTVFCNAEEARDVAALADPALQVQT
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEC
EASAQALAAFEVVIKSPGISPYREEARAAAAQGARFIGGTALWFAEHAQPDGYVPGAICV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRADGHRTALVGNIGQPLLEVLSPQPPPAYWAIELSSYQTGEVG
ECCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPELALVLNLFPEHLDWHGSEAAYVRDKLALVTDGRPRIALLNAADPHLAQLQLPE
CCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCH
SEVRWFNHPDGWHLRGDVVYRGQQPIFDTANVPLPGEHNRRNLCAVLAAVEALGLDAAAL
HHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAALTFRPLPNRLQWLGSVDGIAYVNDSISTTPHASLAALACFAQQRVALLVGGHDRGL
CCHHEEECCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
DWQEFAAHMAQQAPLEIVTMGANGPRIHALLAPLAQSAGFGLHAADDLAHAMQLARSALG
CHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
AQGGVLLLSPGAPSFGVYSDYVARGRHFAQLAGFDPAAISAIPGLGVQ
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MRISQLEGKAVALWGWAREGRAAYRALRQQLPAQPLTVFCNAEEARDVAALADPALQVQT
CCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCEEEC
EASAQALAAFEVVIKSPGISPYREEARAAAAQGARFIGGTALWFAEHAQPDGYVPGAICV
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRADGHRTALVGNIGQPLLEVLSPQPPPAYWAIELSSYQTGEVG
ECCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCC
RSGARPELALVLNLFPEHLDWHGSEAAYVRDKLALVTDGRPRIALLNAADPHLAQLQLPE
CCCCCCCEEHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCH
SEVRWFNHPDGWHLRGDVVYRGQQPIFDTANVPLPGEHNRRNLCAVLAAVEALGLDAAAL
HHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHH
APAALTFRPLPNRLQWLGSVDGIAYVNDSISTTPHASLAALACFAQQRVALLVGGHDRGL
CCHHEEECCCCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCC
DWQEFAAHMAQQAPLEIVTMGANGPRIHALLAPLAQSAGFGLHAADDLAHAMQLARSALG
CHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
AQGGVLLLSPGAPSFGVYSDYVARGRHFAQLAGFDPAAISAIPGLGVQ
CCCCEEEECCCCCCCCCHHHHHHCCCHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA