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The map label for this gene is purN [H]

Identifier: 66767649

GI number: 66767649

Start: 1609359

End: 1610012

Strand: Reverse

Name: purN [H]

Synonym: XC_1322

Alternate gene names: 66767649

Gene position: 1610012-1609359 (Counterclockwise)

Preceding gene: 66767650

Following gene: 77761148

Centisome position: 31.27

GC content: 69.11

Gene sequence:

>654_bases
ATGAGCCTGCGCCTTGCAGTCCTGGCCTCCGGGCGCGGCAGTAATCTGCAGGCCATCCTCGATGCGATTGCCGCCGGGCA
GTTGGCGGCCGAGGTGGCCGGTGTGTTTTCCGACCGGGAACAGGCGCCGGCCTTGCAGAAAGTGGATGCGTCGCGACGCT
GGAGCGCCAGCCCACGCGCGTTCGCCGACCGCGCGGCGTTCGACAGTGCATTGGGCGACGCCATCGCCGCAGTGCAGCCG
GATTGGGTGATCTGCGCCGGCTACATGCGCATCCTGGGCGAGCCTCTGGTGCGCCGCTTCACCGGCCGCATGCTCAACAT
CCATCCCTCGCTGCTGCCCAAATACCGCGGCTTGCACACGCATGCGCGGGCGCTGGAGGCCGGCGATGCCGAGCACGGTG
CCAGCGTGCATCTGGTGGTGCCGGAACTGGATGCCGGCCCGGTGATCGCCCAGGCCCATGTGCCGGTGTTGCCGGACGAC
ACCGCCGAGTCACTGGCGGCGCGGGTGCTGGACCGCGAACACCCGCTGCTGCTGGCCACGCTGCGGTTACTGGCCAGCGG
CCGGGTCACCACGCCCGACGGGCGCGTGCATATCGACGGTCAGTGCCTGTTTACACCACTGCGACTAGAGTCCGCTGGCA
TCGCGCTCGTTTAG

Upstream 100 bases:

>100_bases
ACGCTGGTGGTTCTGCTGCGGCCGCAGGTCAAGGCGTTGTTTGTCACCAACGCGGCAGCCGCTTGAGCTGCGTCTCCGAT
ATAACGATGGGCGTGATTGT

Downstream 100 bases:

>100_bases
CGCGGACACGCGCCCCTCATTCCCTCTCGGACATCCTTGCGGCCCCGTGCCTCGCTCCCGCGCATGAAAGTCCTGCCCTT
CGCTGTCGCGTTGTTCACCA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSLRLAVLASGRGSNLQAILDAIAAGQLAAEVAGVFSDREQAPALQKVDASRRWSASPRAFADRAAFDSALGDAIAAVQP
DWVICAGYMRILGEPLVRRFTGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGPVIAQAHVPVLPDD
TAESLAARVLDREHPLLLATLRLLASGRVTTPDGRVHIDGQCLFTPLRLESAGIALV

Sequences:

>Translated_217_residues
MSLRLAVLASGRGSNLQAILDAIAAGQLAAEVAGVFSDREQAPALQKVDASRRWSASPRAFADRAAFDSALGDAIAAVQP
DWVICAGYMRILGEPLVRRFTGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGPVIAQAHVPVLPDD
TAESLAARVLDREHPLLLATLRLLASGRVTTPDGRVHIDGQCLFTPLRLESAGIALV
>Mature_216_residues
SLRLAVLASGRGSNLQAILDAIAAGQLAAEVAGVFSDREQAPALQKVDASRRWSASPRAFADRAAFDSALGDAIAAVQPD
WVICAGYMRILGEPLVRRFTGRMLNIHPSLLPKYRGLHTHARALEAGDAEHGASVHLVVPELDAGPVIAQAHVPVLPDDT
AESLAARVLDREHPLLLATLRLLASGRVTTPDGRVHIDGQCLFTPLRLESAGIALV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=191, Percent_Identity=40.8376963350785, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI209869995, Length=191, Percent_Identity=40.8376963350785, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI209869993, Length=191, Percent_Identity=40.8376963350785, Blast_Score=137, Evalue=8e-33,
Organism=Escherichia coli, GI1788846, Length=205, Percent_Identity=45.8536585365854, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI1787483, Length=193, Percent_Identity=31.0880829015544, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1788589, Length=117, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=175, Percent_Identity=32, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320616, Length=209, Percent_Identity=30.622009569378, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=43.6170212765958, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23016; Mature: 22885

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLAVLASGRGSNLQAILDAIAAGQLAAEVAGVFSDREQAPALQKVDASRRWSASPRA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCHH
FADRAAFDSALGDAIAAVQPDWVICAGYMRILGEPLVRRFTGRMLNIHPSLLPKYRGLHT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHH
HARALEAGDAEHGASVHLVVPELDAGPVIAQAHVPVLPDDTAESLAARVLDREHPLLLAT
HHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LRLLASGRVTTPDGRVHIDGQCLFTPLRLESAGIALV
HHHHHCCCEECCCCEEEECCEEEEEEEEECCCCEECC
>Mature Secondary Structure 
SLRLAVLASGRGSNLQAILDAIAAGQLAAEVAGVFSDREQAPALQKVDASRRWSASPRA
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCHH
FADRAAFDSALGDAIAAVQPDWVICAGYMRILGEPLVRRFTGRMLNIHPSLLPKYRGLHT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHCCHHH
HARALEAGDAEHGASVHLVVPELDAGPVIAQAHVPVLPDDTAESLAARVLDREHPLLLAT
HHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHH
LRLLASGRVTTPDGRVHIDGQCLFTPLRLESAGIALV
HHHHHCCCEECCCCEEEECCEEEEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]