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The map label for this gene is ptsI [H]

Identifier: 66767631

GI number: 66767631

Start: 1594990

End: 1596720

Strand: Reverse

Name: ptsI [H]

Synonym: XC_1304

Alternate gene names: 66767631

Gene position: 1596720-1594990 (Counterclockwise)

Preceding gene: 66767632

Following gene: 66767630

Centisome position: 31.01

GC content: 67.94

Gene sequence:

>1731_bases
ATGAGTCTGCGCTTGCGCGGACATGCCGCCTCGCGCGGTAACGCATTGGGGCGCGCCCGCGTCCGCCAGAGCCACACGCC
GGAGGTGGCCGAGCAACGCGTGCCGGCCAACCAGGTCGAAACGCAGCTGCAGCGTCTGCACGCGGCCATTGCCGCCGCAC
GCACCGAGATGCAGCAACTGCGCGACCGCCTGCATGGCGCGCTGGCCCGCGAAGTGGGCGAATTCCTCGAACTGCATGCC
CTGTTGCTAGACGACCCGGAACTGCTGCAGGGGCTAGACGATTTGATCCGCACGCACCGCTACAGCGCCGACTACGCGCT
GCGCGTGCAACGCGATCGTCTGGCGGCGGTGTTCGACGGCATGGAAGATGCCTACCTGCGCAGCCGCATGGACGATCTGG
ACCATGTGATCGGGCGCATCCATGCGTTTTTGCACAAGCGCGCGCCGGACCTCAAGGGCGTGGCCGGCGAGATCCTGGTG
TGCGACAACGTGGCACCGTCGGAGCTGGCGCAGCTGCAGGCGCAAGGCGTGGTCGGCATCGTCACCACTGCCGGCAGTGC
GCTCTCGCATAGCGCGATCCTGGCGCGCAGCCTGCACCTGCCCTTGGTGGTCGGCGTGGCCGACGCGGTGCAGCGGGTCA
ACGATGGCGACGTGCTGATCGTCGATGCCGGCTCCGGCCAGGTCATCGTCGACCCCACGCCCGAGCATCTGCGCGACTAC
CGCGAGCGCCTGCGCACGCTGGCCAAGGAACAACGCGAACTGGGCCGGTTGCGCTCCAAGCCGACCCGCACGCGCGACAA
CGTCGACATCACCCTGCTGGCCAATGCCGAGTCGCTGGAAGACGTGGCCAAGGCGCATGCGCTGGGCGCGAGCGGGCTGG
GGCTGTACCGCACCGAATTCCTGTTCCTGCAGCGCAACGAGCTGCCGGACGAAGACGAGCAGTTCCACACTTACCGCGAC
ACCGTGCTGGGCATGAGCGGCCGGCCGGTGACCATCCGTACGCTGGATCTGGGCGCGGACAAGGCCGACCGCACCGGGCT
CACCCTGAGCGATGAGGACAACCCGGCGCTGGGCCTGCGCGGGGTGCGCCTGTCGCTTGCCCGGCCGGCGGTGGCCCAGG
CACAGCTGCGCGCGATCCTGCGCGCGTCCGGCTACGGCCCGGTGCGCATCCTGGTGCCGATGGTCAGCGGGCGCGAGGAA
ATCCTGTTGGTGCGCAAACAGCTGAAAAAGCTCACCGCGCAGCTGCGCGACGAAGGCCACGAGATCGCCGAGCACATTCC
GCTCGGCGCGATGATCGAGGTGCCGGCGGCGGCATTGGCGCTGGACTGCTTCATCGACGACATCGACTTTTTGTCGATCG
GCACCAACGACCTGGTGCAATACCTGCTGGCGGTGGACCGCAACAACGAAGCGCTAGGCGAGCTGTATTCGCCGTTGCAT
CCGGCGGTGCTGCGATTGATTGCGCAGGTCATCGCCACCGGGCGGGCGCACAACAAGCCGGTGGCCGTGTGTGGCGAAAT
CGCCGGCGACCCGCGCTTCGTGGCGATGCTGCTGGCGTTGGGCCTCACCGAGTTCAGCCTGCATCCGGCCACCTTGCTGG
AAGTACGCCGCGCGGTGCGCGACCTGCATCTGGGCCAGTTGCGCGTGCACGGCGAAAAACTGCTGCGCGCGCGCGACCGC
AAGAGCATCGAAAAATGGCTGGCAAGCGTGGCGCAAGGCCTGCTGCACTGA

Upstream 100 bases:

>100_bases
TTGGTGGACCTGTTCGAGCGCCGCTTCGACGAGGACAGCTGAGCATGCGCTGCGTCGGTGCCGTGCTGACCGACACGGCG
CGGCTGCGCCAAGGCCGCGC

Downstream 100 bases:

>100_bases
GTTGCGCCACACCCGCGCCCGCAGCGCGCGGGCACCGCTGCACCTGCACTGTATGTTTCGTCACCGTGCGCAAGCGATAA
TGCCCTGCTGATCCAGCACC

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSLRLRGHAASRGNALGRARVRQSHTPEVAEQRVPANQVETQLQRLHAAIAAARTEMQQLRDRLHGALAREVGEFLELHA
LLLDDPELLQGLDDLIRTHRYSADYALRVQRDRLAAVFDGMEDAYLRSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILV
CDNVAPSELAQLQAQGVVGIVTTAGSALSHSAILARSLHLPLVVGVADAVQRVNDGDVLIVDAGSGQVIVDPTPEHLRDY
RERLRTLAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVAKAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRD
TVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREE
ILLVRKQLKKLTAQLRDEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
PAVLRLIAQVIATGRAHNKPVAVCGEIAGDPRFVAMLLALGLTEFSLHPATLLEVRRAVRDLHLGQLRVHGEKLLRARDR
KSIEKWLASVAQGLLH

Sequences:

>Translated_576_residues
MSLRLRGHAASRGNALGRARVRQSHTPEVAEQRVPANQVETQLQRLHAAIAAARTEMQQLRDRLHGALAREVGEFLELHA
LLLDDPELLQGLDDLIRTHRYSADYALRVQRDRLAAVFDGMEDAYLRSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILV
CDNVAPSELAQLQAQGVVGIVTTAGSALSHSAILARSLHLPLVVGVADAVQRVNDGDVLIVDAGSGQVIVDPTPEHLRDY
RERLRTLAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVAKAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRD
TVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREE
ILLVRKQLKKLTAQLRDEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
PAVLRLIAQVIATGRAHNKPVAVCGEIAGDPRFVAMLLALGLTEFSLHPATLLEVRRAVRDLHLGQLRVHGEKLLRARDR
KSIEKWLASVAQGLLH
>Mature_575_residues
SLRLRGHAASRGNALGRARVRQSHTPEVAEQRVPANQVETQLQRLHAAIAAARTEMQQLRDRLHGALAREVGEFLELHAL
LLDDPELLQGLDDLIRTHRYSADYALRVQRDRLAAVFDGMEDAYLRSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVC
DNVAPSELAQLQAQGVVGIVTTAGSALSHSAILARSLHLPLVVGVADAVQRVNDGDVLIVDAGSGQVIVDPTPEHLRDYR
ERLRTLAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVAKAHALGASGLGLYRTEFLFLQRNELPDEDEQFHTYRDT
VLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLRGVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEI
LLVRKQLKKLTAQLRDEGHEIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLHP
AVLRLIAQVIATGRAHNKPVAVCGEIAGDPRFVAMLLALGLTEFSLHPATLLEVRRAVRDLHLGQLRVHGEKLLRARDRK
SIEKWLASVAQGLLH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=553, Percent_Identity=36.8896925858951, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI1788726, Length=576, Percent_Identity=31.9444444444444, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1789193, Length=536, Percent_Identity=33.7686567164179, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI48994992, Length=411, Percent_Identity=32.8467153284672, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1787994, Length=427, Percent_Identity=25.0585480093677, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI226510935, Length=188, Percent_Identity=27.6595744680851, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63285; Mature: 63153

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLRGHAASRGNALGRARVRQSHTPEVAEQRVPANQVETQLQRLHAAIAAARTEMQQL
CCEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDRLHGALAREVGEFLELHALLLDDPELLQGLDDLIRTHRYSADYALRVQRDRLAAVFDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCC
MEDAYLRSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVCDNVAPSELAQLQAQGVVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
VTTAGSALSHSAILARSLHLPLVVGVADAVQRVNDGDVLIVDAGSGQVIVDPTPEHLRDY
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHH
RERLRTLAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVAKAHALGASGLGLYRTEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHE
LFLQRNELPDEDEQFHTYRDTVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLR
EEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCC
GVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEILLVRKQLKKLTAQLRDEGH
CEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
HHHHHCCCCCHHHCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCC
PAVLRLIAQVIATGRAHNKPVAVCGEIAGDPRFVAMLLALGLTEFSLHPATLLEVRRAVR
HHHHHHHHHHHHCCCCCCCCCEEEHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
DLHLGQLRVHGEKLLRARDRKSIEKWLASVAQGLLH
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLRLRGHAASRGNALGRARVRQSHTPEVAEQRVPANQVETQLQRLHAAIAAARTEMQQL
CEEECCCCCCCCCHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDRLHGALAREVGEFLELHALLLDDPELLQGLDDLIRTHRYSADYALRVQRDRLAAVFDG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCC
MEDAYLRSRMDDLDHVIGRIHAFLHKRAPDLKGVAGEILVCDNVAPSELAQLQAQGVVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEE
VTTAGSALSHSAILARSLHLPLVVGVADAVQRVNDGDVLIVDAGSGQVIVDPTPEHLRDY
EECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCHHHHHHH
RERLRTLAKEQRELGRLRSKPTRTRDNVDITLLANAESLEDVAKAHALGASGLGLYRTEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHE
LFLQRNELPDEDEQFHTYRDTVLGMSGRPVTIRTLDLGADKADRTGLTLSDEDNPALGLR
EEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCCCC
GVRLSLARPAVAQAQLRAILRASGYGPVRILVPMVSGREEILLVRKQLKKLTAQLRDEGH
CEEHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
EIAEHIPLGAMIEVPAAALALDCFIDDIDFLSIGTNDLVQYLLAVDRNNEALGELYSPLH
HHHHHCCCCCHHHCCHHHHHHHHHHCCCHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCC
PAVLRLIAQVIATGRAHNKPVAVCGEIAGDPRFVAMLLALGLTEFSLHPATLLEVRRAVR
HHHHHHHHHHHHCCCCCCCCCEEEHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHH
DLHLGQLRVHGEKLLRARDRKSIEKWLASVAQGLLH
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]