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The map label for this gene is ampR [H]

Identifier: 66767446

GI number: 66767446

Start: 1355266

End: 1356141

Strand: Reverse

Name: ampR [H]

Synonym: XC_1118

Alternate gene names: 66767446

Gene position: 1356141-1355266 (Counterclockwise)

Preceding gene: 66767449

Following gene: 66767443

Centisome position: 26.34

GC content: 70.21

Gene sequence:

>876_bases
ATGCCCAGACCGCGTCTCCCGCTCAATGCCCTGCGCGCGTTCGAGGCCGCCGCACGCCATCAGAACCTGACCCGCGCGGC
CGGCGAGCTGTGCGTCAGCCAGGCCGCGCTCAGCCACCAGATCAAGGCATTGGAGCAACAGCTGGGCACCAGCCTGTTCC
ACCGCCTGCCGCGCGGGGTCGCGCTCACCGACGAGGGCGCCGCGCTGGCGCCGGTGCTGGGCGACGCATTCGACCGCATT
GCCGCCACGCTGGAGCGCTTTGCCGGCGGCCGCTATCGCGAAGTGCTCAGCTTGGGCGTGGTGGCCACGTTTGCCACCGG
CTGGTTGCTGCCGCGCGTGGCCGCCTTCCACGCGGCACATCCGGACATCGAACTGCGCCTGTCGACCCACAACAATCGGG
TGGATCTGGCCGGCGAAGGGCTGGATCTGGCAATCCGCTTCGGCGACGGCGACTGGCAGGGGCAGATTGCGCATGCCGTG
ATGGAGGCACCGTTTGCGCCGGTATGCGCCCCGGCGATGGCGCGTGCCTTGCACACGCCTGCCGATCTGGCCCACGTGCC
GTTGCTGCGTTCGTACCGGCCGGACGAATGGGCACAGTGGTTTCGTGCTGCCGGCACCTCGGAGGTGCCTGCGCGTGGCG
CGATGTTCGATTCGTCGCTGGCACTGGCCAGTGCGGCCGCGGCTGGCGCTGGTGTGGCGTTGTTGCCGCTGCGCTTGTTT
GCCCAGGACCTGGGCGCGGGGCGATTGGCCTGCCCATTCCCGATCGAGATCGATGCGGGGCGCTACTGGTTGACCCGGTT
GCGCTCGCGCCCGGAAAGCGACGCCGAAGCCCGGCTGCGCGCCTGGCTGCTGGCCGAACAGCAGCGCGCCGATTGA

Upstream 100 bases:

>100_bases
GGCGCTGCCTCCTTGAGAGAGCCCGCAGTATCGGGGCCGGCGCGCTGGCCGACCACCGCAATGTTTCGATCCCTGGCATG
AATTGAACTAATCTCCTGGC

Downstream 100 bases:

>100_bases
GCAGCGTCGCCGCGTGCCACGTTCAACGGCGCGCGGACGCCTTCCCCACCTGCAACGCAAAGTGGCGAAACGCTTCGTAC
TGCGCCCTGTCGGCGAACCA

Product: transcriptional activator ampR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPRPRLPLNALRAFEAAARHQNLTRAAGELCVSQAALSHQIKALEQQLGTSLFHRLPRGVALTDEGAALAPVLGDAFDRI
AATLERFAGGRYREVLSLGVVATFATGWLLPRVAAFHAAHPDIELRLSTHNNRVDLAGEGLDLAIRFGDGDWQGQIAHAV
MEAPFAPVCAPAMARALHTPADLAHVPLLRSYRPDEWAQWFRAAGTSEVPARGAMFDSSLALASAAAAGAGVALLPLRLF
AQDLGAGRLACPFPIEIDAGRYWLTRLRSRPESDAEARLRAWLLAEQQRAD

Sequences:

>Translated_291_residues
MPRPRLPLNALRAFEAAARHQNLTRAAGELCVSQAALSHQIKALEQQLGTSLFHRLPRGVALTDEGAALAPVLGDAFDRI
AATLERFAGGRYREVLSLGVVATFATGWLLPRVAAFHAAHPDIELRLSTHNNRVDLAGEGLDLAIRFGDGDWQGQIAHAV
MEAPFAPVCAPAMARALHTPADLAHVPLLRSYRPDEWAQWFRAAGTSEVPARGAMFDSSLALASAAAAGAGVALLPLRLF
AQDLGAGRLACPFPIEIDAGRYWLTRLRSRPESDAEARLRAWLLAEQQRAD
>Mature_290_residues
PRPRLPLNALRAFEAAARHQNLTRAAGELCVSQAALSHQIKALEQQLGTSLFHRLPRGVALTDEGAALAPVLGDAFDRIA
ATLERFAGGRYREVLSLGVVATFATGWLLPRVAAFHAAHPDIELRLSTHNNRVDLAGEGLDLAIRFGDGDWQGQIAHAVM
EAPFAPVCAPAMARALHTPADLAHVPLLRSYRPDEWAQWFRAAGTSEVPARGAMFDSSLALASAAAAGAGVALLPLRLFA
QDLGAGRLACPFPIEIDAGRYWLTRLRSRPESDAEARLRAWLLAEQQRAD

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=285, Percent_Identity=35.0877192982456, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=32.2916666666667, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=33.2167832167832, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787128, Length=256, Percent_Identity=28.90625, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI145693193, Length=269, Percent_Identity=28.996282527881, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787589, Length=237, Percent_Identity=27.8481012658228, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=26.551724137931, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=34.2657342657343, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=256, Percent_Identity=24.609375, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1787601, Length=296, Percent_Identity=23.3108108108108, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=27.3858921161826, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31378; Mature: 31247

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPRLPLNALRAFEAAARHQNLTRAAGELCVSQAALSHQIKALEQQLGTSLFHRLPRGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALTDEGAALAPVLGDAFDRIAATLERFAGGRYREVLSLGVVATFATGWLLPRVAAFHAAH
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIELRLSTHNNRVDLAGEGLDLAIRFGDGDWQGQIAHAVMEAPFAPVCAPAMARALHTP
CCEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
ADLAHVPLLRSYRPDEWAQWFRAAGTSEVPARGAMFDSSLALASAAAAGAGVALLPLRLF
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AQDLGAGRLACPFPIEIDAGRYWLTRLRSRPESDAEARLRAWLLAEQQRAD
HHHHCCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRPRLPLNALRAFEAAARHQNLTRAAGELCVSQAALSHQIKALEQQLGTSLFHRLPRGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALTDEGAALAPVLGDAFDRIAATLERFAGGRYREVLSLGVVATFATGWLLPRVAAFHAAH
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIELRLSTHNNRVDLAGEGLDLAIRFGDGDWQGQIAHAVMEAPFAPVCAPAMARALHTP
CCEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
ADLAHVPLLRSYRPDEWAQWFRAAGTSEVPARGAMFDSSLALASAAAAGAGVALLPLRLF
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
AQDLGAGRLACPFPIEIDAGRYWLTRLRSRPESDAEARLRAWLLAEQQRAD
HHHHCCCCCCCCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]