BacMap

Definition	Xanthomonas campestris pv. campestris str. 8004 chromosome, complete genome.
Accession	NC_007086
Length	5,148,708

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The map label for this gene is dkgB [H]

Identifier: 66766623

GI number: 66766623

Start: 331709

End: 332512

Strand: Reverse

Name: dkgB [H]

Synonym: XC_0279

Alternate gene names: 66766623

Gene position: 332512-331709 (Counterclockwise)

Preceding gene: 66766624

Following gene: 66766622

Centisome position: 6.46

GC content: 64.18

Gene sequence:

>804_bases
ATGACTGTCCCCGCATTTGGCCTCGGTACGTTTCGCCTCAAGGACCAGGTTGTCATCGACTCGGTGCGCAACGCACTGGC
GCTGGGGTATCGCGCCATCGATACCGCACAGATCTACGACAACGAAGCGCAGGTTGGCCAGGCCATTGCCGATGCCGGCG
TGCCGCGCGATCAGCTGTTTGTGACCACCAAGATCTGGGTGGACCGGTTCAGCCGCGACACGCTGCAACCCAGCCTGCAG
GAAAGCCTGCGCAAGCTCGGCACCGATTATGTCGACCTCACCCTGATCCACTGGCCCTCGCCCAAGGACCAGGTGCCGAT
GCGCGAGTATCTGGAGGCGCTGGCCGATGCCAAGGCGCAGGGGCTGACCCGCGCGATCGGTGTCTCCAACTTCACCATTG
CGCTCACGCGCCAGGCAATCGAGATCCTCGGCGCCGATGCGATCGCCACCAACCAGATCGAAGTGCATCCGTACCTGCAG
AACCGCACGCTGATCGCGTTCCTGCGCGAGCAGGGCATCCATGTCACCTCGTACATGACCCTGGCCGTGGGCGAAGTGCT
CAAGGATCCGGTAATCCAGGCGATCGCCAGGCGCCACGACGCCACGCCGGCGCAGGTCACGCTGGCCTGGGCCCTGCAGC
AGGGCTATTCGGTAATTCCGTCCTCGACCAAGCGGGCGAATCTGGAAAGCAACCTCAAGGCGCAGAGCCTGCACCTGAGT
GAGCAGGACATGGCCGAGATCGCCGCGCTGGACCGTGGCGAACGCCTGGCCAACCCGAAAGCGATTGCGCCCGACTGGGA
CTGA

Upstream 100 bases:

>100_bases
AACTTCGGCAAGGCCGTGGTCGCACTCGACTGAGTCGCGCTGCCATACCTGCATCCGTATCACTGCTTCATTTACCACCT
CCCAACACGCAAGGAACCTC

Downstream 100 bases:

>100_bases
GCCTGACTGCGACACACGCGAGGGTTTGCATCCGTACCGGCACGCTGCCGGTGCGGGTTTTGCGGTACTGCTGCACCGCT
CGTCGCCATGCGCTGGCTGC

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTVPAFGLGTFRLKDQVVIDSVRNALALGYRAIDTAQIYDNEAQVGQAIADAGVPRDQLFVTTKIWVDRFSRDTLQPSLQ
ESLRKLGTDYVDLTLIHWPSPKDQVPMREYLEALADAKAQGLTRAIGVSNFTIALTRQAIEILGADAIATNQIEVHPYLQ
NRTLIAFLREQGIHVTSYMTLAVGEVLKDPVIQAIARRHDATPAQVTLAWALQQGYSVIPSSTKRANLESNLKAQSLHLS
EQDMAEIAALDRGERLANPKAIAPDWD

Sequences:

>Translated_267_residues
MTVPAFGLGTFRLKDQVVIDSVRNALALGYRAIDTAQIYDNEAQVGQAIADAGVPRDQLFVTTKIWVDRFSRDTLQPSLQ
ESLRKLGTDYVDLTLIHWPSPKDQVPMREYLEALADAKAQGLTRAIGVSNFTIALTRQAIEILGADAIATNQIEVHPYLQ
NRTLIAFLREQGIHVTSYMTLAVGEVLKDPVIQAIARRHDATPAQVTLAWALQQGYSVIPSSTKRANLESNLKAQSLHLS
EQDMAEIAALDRGERLANPKAIAPDWD
>Mature_266_residues
TVPAFGLGTFRLKDQVVIDSVRNALALGYRAIDTAQIYDNEAQVGQAIADAGVPRDQLFVTTKIWVDRFSRDTLQPSLQE
SLRKLGTDYVDLTLIHWPSPKDQVPMREYLEALADAKAQGLTRAIGVSNFTIALTRQAIEILGADAIATNQIEVHPYLQN
RTLIAFLREQGIHVTSYMTLAVGEVLKDPVIQAIARRHDATPAQVTLAWALQQGYSVIPSSTKRANLESNLKAQSLHLSE
QDMAEIAALDRGERLANPKAIAPDWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=291, Percent_Identity=33.6769759450172, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI5174391, Length=291, Percent_Identity=33.6769759450172, Blast_Score=162, Evalue=2e-40,
Organism=Homo sapiens, GI223468663, Length=288, Percent_Identity=32.6388888888889, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=32.1917808219178, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI24497585, Length=292, Percent_Identity=32.1917808219178, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI45446745, Length=289, Percent_Identity=31.8339100346021, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI4503285, Length=289, Percent_Identity=31.8339100346021, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI310109920, Length=289, Percent_Identity=31.8339100346021, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI310109922, Length=272, Percent_Identity=31.9852941176471, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=31.023102310231, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI24497583, Length=300, Percent_Identity=32, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=30.8474576271186, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=31.8518518518519, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI4502049, Length=290, Percent_Identity=33.448275862069, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI300116273, Length=272, Percent_Identity=30.8823529411765, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=28.5198555956679, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=38.4615384615385, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=38.4615384615385, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=29.8701298701299, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=67.0411985018727, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI87082198, Length=261, Percent_Identity=37.9310344827586, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1788081, Length=270, Percent_Identity=30.3703703703704, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787674, Length=256, Percent_Identity=30.859375, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788070, Length=319, Percent_Identity=26.9592476489028, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081735, Length=272, Percent_Identity=27.2058823529412, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48994888, Length=278, Percent_Identity=26.2589928057554, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17561298, Length=269, Percent_Identity=31.9702602230483, Blast_Score=153, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17550248, Length=284, Percent_Identity=32.3943661971831, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17537075, Length=293, Percent_Identity=34.4709897610922, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17552492, Length=268, Percent_Identity=30.2238805970149, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71998625, Length=262, Percent_Identity=32.4427480916031, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17537077, Length=289, Percent_Identity=32.5259515570934, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=31.9727891156463, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=31.3588850174216, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17561300, Length=263, Percent_Identity=30.0380228136882, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538386, Length=269, Percent_Identity=30.8550185873606, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=273, Percent_Identity=31.1355311355311, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=29.553264604811, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324694, Length=292, Percent_Identity=35.2739726027397, Blast_Score=178, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6320576, Length=279, Percent_Identity=37.9928315412186, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=30.0653594771242, Blast_Score=142, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322556, Length=280, Percent_Identity=29.6428571428571, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6319625, Length=290, Percent_Identity=31.7241379310345, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320079, Length=249, Percent_Identity=28.1124497991968, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24662789, Length=297, Percent_Identity=34.6801346801347, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=32.7586206896552, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=34.3333333333333, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=31.2302839116719, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI20129731, Length=281, Percent_Identity=32.3843416370107, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24663317, Length=287, Percent_Identity=30.3135888501742, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356425, Length=286, Percent_Identity=30.0699300699301, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45553081, Length=283, Percent_Identity=29.6819787985866, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=31.8339100346021, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24662781, Length=288, Percent_Identity=31.9444444444444, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=28.6821705426357, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29454; Mature: 29323

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPAFGLGTFRLKDQVVIDSVRNALALGYRAIDTAQIYDNEAQVGQAIADAGVPRDQLF
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCHHEE
VTTKIWVDRFSRDTLQPSLQESLRKLGTDYVDLTLIHWPSPKDQVPMREYLEALADAKAQ
EEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
GLTRAIGVSNFTIALTRQAIEILGADAIATNQIEVHPYLQNRTLIAFLREQGIHVTSYMT
HHHHHHCCCCEEEHHHHHHHHHHCCCCEECCEEEEEEECCCCHHEEHHHHCCCCHHHHHH
LAVGEVLKDPVIQAIARRHDATPAQVTLAWALQQGYSVIPSSTKRANLESNLKAQSLHLS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHCCCHHHCCCC
EQDMAEIAALDRGERLANPKAIAPDWD
HHHHHHHHHHCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVPAFGLGTFRLKDQVVIDSVRNALALGYRAIDTAQIYDNEAQVGQAIADAGVPRDQLF
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCHHEE
VTTKIWVDRFSRDTLQPSLQESLRKLGTDYVDLTLIHWPSPKDQVPMREYLEALADAKAQ
EEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
GLTRAIGVSNFTIALTRQAIEILGADAIATNQIEVHPYLQNRTLIAFLREQGIHVTSYMT
HHHHHHCCCCEEEHHHHHHHHHHCCCCEECCEEEEEEECCCCHHEEHHHHCCCCHHHHHH
LAVGEVLKDPVIQAIARRHDATPAQVTLAWALQQGYSVIPSSTKRANLESNLKAQSLHLS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHCCCHHHCCCC
EQDMAEIAALDRGERLANPKAIAPDWD
HHHHHHHHHHCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]